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Total 2565 results found since Jan 2013.

Synergistic effect of siRNA ‐mediated silencing of TGF‐β R1 and TGF‐β R2 genes on the proliferation and apoptosis of penile urethral epithelial cells in hypospadiac male rats
This study ultimately demonstrated that the silencing of TGF‐β R1 and TGF‐β R2 genes could promote cell proliferation and inhibit apoptosis of penile UECs in hypospadiac male rats. This article is protected by copyright. All rights reserved
Source: Journal of Cellular Biochemistry - August 4, 2017 Category: Biochemistry Authors: Chao Wang, Yan ‐Yan Qiao Tags: Article Source Type: research

Preparation of siRNA encapsulated nanoliposomes suitable for siRNA delivery by simply discontinuous mixing.
In this study, we prepared nanoliposomes encapsulating siRNA by simply discontinuous mixing of lipids in ethanol/ether/water mixture and acidic siRNA solution without use of special equipment. The simple mixing siRNA/liposomal particles (siRNA/SMLs) prepared using ethanol/ether/water (3:1:1) mixture showed 120.4 ± 20.2 nm particle size, 0.174 ± 0.033 polydispersity and 86.5 ± 2.76% siRNA encapsulation rate. In addition, the SMLs almost completely protected the encapsulated siRNA from RNase A digestion. Coupling of anti-human epidermal growth factor receptor (EGFR) Fab' to siRNA/SMLs enhanced EGFR-specific ...
Source: Biochimica et Biophysica Acta - February 28, 2018 Category: Biochemistry Authors: Mokhtarieh AA, Lee J, Kim S, Lee MK Tags: Biochim Biophys Acta Source Type: research

Preparation of siRNA encapsulated nanoliposomes suitable for siRNA delivery by simply discontinuous mixing
In this study, we prepared nanoliposomes encapsulating siRNA by simply discontinuous mixing of lipids in ethanol/ether/water mixture and acidic siRNA solution without use of special equipment. The simple mixing siRNA/liposomal particles (siRNA/SMLs) prepared using ethanol/ether/water (3:1:1) mixture showed 120.4 ± 20.2 nm particle size, 0.174 ± 0.033 polydispersity and 86.5 ± 2.76% siRNA encapsulation rate. In addition, the SMLs almost completely protected the encapsulated siRNA from RNase A digestion. Coupling of anti-human epidermal growth factor receptor (EGFR) Fab′ to siRNA/SMLs enhanced EGFR-specifi...
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - March 6, 2018 Category: Biochemistry Source Type: research

Antibody-modified lipid nanoparticles for selective delivery of siRNA to tumors expressing membrane-anchored form of HB-EGF.
Abstract An Fab' antibody against heparin-binding epidermal growth factor-like growth factor (HB-EGF) was applied to achieve advanced tumor-targeted delivery of siRNA. Lipid nanoparticles (LNP) encapsulating siRNA (LNP-siRNA) were prepared, pegylated, and surface modified with Fab' fragments of anti-HB-EGF antibody (αHB-EGF LNP-siRNA). αHB-EGF LNP-siRNA showed high-binding affinity to recombinant human HB-EGF in a Biacore assay. In addition, αHB-EGF LNP-siRNA selectively associated with cells expressing HB-EGF in vitro. Confocal microscopic images showed that siRNA formulated in αHB-EGF LNP-siRNA was efficient...
Source: Biochemical and Biophysical Research communications - May 19, 2014 Category: Biochemistry Authors: Okamoto A, Asai T, Kato H, Ando H, Minamino T, Mekada E, Oku N Tags: Biochem Biophys Res Commun Source Type: research

siRNA blocking the RAS signalling pathway and inhibits the growth of oesophageal squamous cell carcinoma in nude mice
The aim of this study was to study RAS‐siRNA blocking RAS pathway and suppressing cell growth in human oesophageal squamous cell carcinoma in nude mice. The methods in this study was to construct RAS‐siRNA expression vector, establish 40 oesophageal squamous cell carcinoma xenograft animal models and divided them into five groups: control group, siRNA control group, RAS‐siRNA group, paclitaxel group and RAS‐siRNA and paclitaxel group. We observed tumour growth in nude mice, studied histology by HE staining, tumour growth inhibition by TUNEL assay and detected the RAS, MAPK and cyclin D1 protein expression by immuno...
Source: Cell Biochemistry and Function - November 28, 2014 Category: Biochemistry Authors: Xinjie Wang, Yuling Zheng, Qingxia Fan, Xudong Zhang, Yonggang Shi Tags: Research Article Source Type: research

Cholesterol-grafted chitosan micelles as a nanocarrier system for drug-siRNA co-delivery to the lung cancer cells.
Abstract Combined delivery of a therapeutic small interfering RNA (siRNA) and a chemotherapeutic agent to cancer cells is promising as anticancer therapy, which could offer enhanced cell killing potential and low side effect. However, simultaneous delivery to tumor is challenging. In our study, cholesterol-modified low molecular weight chitosan (MW ~ 15 kDa) was employed as a self-assembled delivery system for both siRNA and a hydrophobic chemotherapeutic agent, curcumin to cancer cells. The siRNA/curcumin loaded nanoparticles (C-CCM/siRNA) were physico-chemically characterized for particle size (165 ± ...
Source: International Journal of Biological Macromolecules - June 25, 2018 Category: Biochemistry Authors: Muddineti OS, Shah A, Rompicharla SVK, Ghosh B, Biswas S Tags: Int J Biol Macromol Source Type: research

PEGylated DC-Chol/DOPE cationic liposomes containing KSP siRNA as a systemic siRNA delivery Carrier for ovarian cancer therapy.
Abstract Although siRNA-mediated downregulation technology has been highly successful in suppressing the expression of any disease-related gene, systemic delivery of siRNA for the clinical applications remains challenging, especially in the use of cancer therapy. DC-Chol/DOPE cationic liposomes as one of the most attractive vehicles for gene delivery have been widely exploited for transfection of siRNA into cells, but complexity of systemic delivery has allowed only their direct injection into local targets due to the formation of aggregations with negatively-charged blood components. Herein, we demonstrate the ef...
Source: Biochemical and Biophysical Research communications - July 23, 2018 Category: Biochemistry Authors: Lee J, Ahn HJ Tags: Biochem Biophys Res Commun Source Type: research

Surface modified HK:siRNA nanoplexes with enhanced pharmacokinetics and tumor growth inhibition.
Abstract We characterized in this study the pharmacokinetics and antitumor efficacy of histidine-lysine (HK):siRNA nanoplexes modified with PEG and a cyclic RGD (cRGD) ligand targeting αvβ3 and αvβ5 integrins. With non-invasive imaging, systemically administered surface modified HK:siRNA nanoplexes showed nearly 4-fold greater blood levels, 40% higher accumulation in tumor tissue, and 60% lower luciferase activity than unmodified HK:siRNA nanoplexes. We then determined whether the surface modified HK:siRNA nanoplex carrier was more effective in reducing MDA-MB-435 tumor growth with an siRNA targeting Raf-1. Re...
Source: Biomacromolecules - January 29, 2013 Category: Biochemistry Authors: Chou ST, Leng Q, Scaria P, Kahn JD, Tricoli LJ, Woodle M, Mixson AJ Tags: Biomacromolecules Source Type: research

Monodispersed Brush-like Conjugated Polyelectrolyte Nanoparticles with Efficient and Visualized SiRNA Delivery for Gene Silencing.
Abstract RNA interference is supposed to be one of the most powerful technologies for suppression of genes and treatment of diverse human diseases while the safely delivery and visualization of siRNA were still challenging. In this text, a novel type of monodispersed conjugated polymer nanoparticles PFNBr with brush-like molecular structure was introduced into siRNA delivery system. The nanoparticles in the delivery system simply and conveniently acquired dual functions which can not only carry high amount of siRNA to penetrate intracellularly for knockdown targeted mRNA but also act as signal agents for siRNA tra...
Source: Biomacromolecules - September 16, 2013 Category: Biochemistry Authors: Jiang R, Lu XM, Yang M, Deng W, Fan Q, Huang W Tags: Biomacromolecules Source Type: research

Formulation of glutathione responsive anti-proliferative nanoparticles from thiolated Akt1 siRNA and disulfide-crosslinked PEI for efficient anti-cancer gene therapy
In this study, thiol-modified siRNA (SH-siRNA) was delivered by bioreducible polyethylenimine (ssPEI), to enhance physicochemical properties of polyplexes and function of siRNA through disulfide bonding between SH-siRNA and ssPEI. The ssPEI was utilized to deliver Akt1 SH-siRNA for suppression of Akt1 mRNA and blockage of Akt1 protein translation, resulting in reduced cellular proliferation and the induction of apoptosis. Disulfide bondings between the ssPEI and SH-siRNA through thiol groups in both were confirmed by DTT treatment. Complexation between ssPEI and Akt1SH-siRNA was enhanced and reduced surface charge of ssPEI...
Source: Colloids and Surfaces B: Biointerfaces - January 9, 2015 Category: Biochemistry Source Type: research

Transcutaneous iontophoretic delivery of STAT3 siRNA using layer-by-layer chitosan coated gold nanoparticles to treat melanoma
In conclusion, layer-by-layer chitosan coated AuNP can be developed as a carrier for iontophoretic delivery of STAT3 siRNA to treat melanoma. Graphical abstract
Source: Colloids and Surfaces B: Biointerfaces - June 16, 2016 Category: Biochemistry Source Type: research

pH-Responsive Dynaplexes as Potent Apoptosis Inductors by Intracellular Delivery of Survivin siRNA
Biomacromolecules. 2023 Jul 31. doi: 10.1021/acs.biomac.3c00424. Online ahead of print.ABSTRACTGene knockdown by siRNA offers an unrestricted choice of targets and specificity based on the principle of complementary Watson-Crick base pairing with mRNA. However, the negative charge, large molecular size, and susceptibility to enzymatic degradation of siRNA impede its successful transfection, hence limiting its potential for therapeutic use. The development of efficient and safe siRNA transfection agents is, therefore, critical for siRNA-based therapy. Herein, we developed a protein-based biodynamic polymer (biodynamer) that...
Source: Biomacromolecules - July 31, 2023 Category: Biochemistry Authors: Yun Liu Salma Ashmawy Lorenz Latta Agnes-Valencia Weiss Alexander F Kiefer Sarah Nasr Brigitta Loretz Anna K H Hirsch Sangeun Lee Claus-Michael Lehr Source Type: research

A biodegradable stearylated peptide with internal disulfide bonds for efficient delivery of siRNA in vitro and in vivo.
In this study, a stearyl-peptide (SHR) was synthesized using arginine, histidine, cysteine and stearyl moiety. Further, the stearyl-peptides were cross linked by disulfide bonds to obtain cross-linked polypeptides (SHRss) with different molecular weight (SHRss1, SHRss2, SHRss3, SHRss4). The SHRss could effectively condense small interfering RNA (siRNA) into polyplexes with a hydrodynamic size of 100-300 nm and zeta potential of 20-40 mV. Flow cytometry and confocal laser scanning microscope studies revealed high cellular uptake and rapid dissociation behavior of SHRss2/siRNA complexes. Long-lasting high concentration of si...
Source: Biomacromolecules - February 16, 2015 Category: Biochemistry Authors: Tai Z, Wang X, Tian J, Gao Y, Zhang L, Yao C, Wu X, Zhang W, Zhu Q, Gao S Tags: Biomacromolecules Source Type: research

Development of Guanidinium-rich Protein Mimics for Efficient siRNA Delivery into Human T Cells.
Abstract RNA interference is gaining attention as a means to explore new molecular pathways and for its potential as a therapeutic; however, its application in immortal and primary T cells is limited due to challenges with efficient delivery in these cells types. Herein, we report the development of guanidinium-rich protein transduction domain mimics (PTDMs) based on a ring-opening metathesis polymerization scaffold that delivers siRNA into Jurkat T cells and human peripheral blood mononuclear cells (hPBMCs). Homopolymer and block copolymer PTDMs with varying numbers of guanidinium moieties were designed and teste...
Source: Biomacromolecules - August 31, 2015 Category: Biochemistry Authors: deRonde BM, Torres JA, Minter LM, Tew GN Tags: Biomacromolecules Source Type: research