Monodispersed Brush-like Conjugated Polyelectrolyte Nanoparticles with Efficient and Visualized SiRNA Delivery for Gene Silencing.

Monodispersed Brush-like Conjugated Polyelectrolyte Nanoparticles with Efficient and Visualized SiRNA Delivery for Gene Silencing. Biomacromolecules. 2013 Sep 16; Authors: Jiang R, Lu XM, Yang M, Deng W, Fan Q, Huang W Abstract RNA interference is supposed to be one of the most powerful technologies for suppression of genes and treatment of diverse human diseases while the safely delivery and visualization of siRNA were still challenging. In this text, a novel type of monodispersed conjugated polymer nanoparticles PFNBr with brush-like molecular structure was introduced into siRNA delivery system. The nanoparticles in the delivery system simply and conveniently acquired dual functions which can not only carry high amount of siRNA to penetrate intracellularly for knockdown targeted mRNA but also act as signal agents for siRNA tracking and cellular imaging. Resulted from the high density side chains with positive charges and more extended conformation of the spatial structure, PFNBr nanoparticles as nanocarrier for siRNA provided outstanding capture ability (1 mol polymer to more than 32.5 mol siRNA) and enhanced protection of siRNA molecules from degradation. As results of the outstanding carrying capacity of PFNBr, here the concentration of carrier in the working platform was lowered from the level of μmol/L to nmol/L compared with other conjugated polymer delivery systems. And meanwhile, this system acquired high gene silence efficiency and ...
Source: Biomacromolecules - Category: Biochemistry Authors: Tags: Biomacromolecules Source Type: research