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Condition: Polycystic Ovary Syndrome

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Total 20 results found since Jan 2013.

Spexin role in human granulosa cells physiology and PCOS: expression and negative impact on steroidogenesis and proliferation
Biol Reprod. 2023 Sep 2:ioad108. doi: 10.1093/biolre/ioad108. Online ahead of print.ABSTRACTSpexin (SPX) is a novel neuropeptide and adipokine negatively correlated with obesity and insulin resistance. A recent study investigated expression and regulatory function of SPX in the hypothalamus and pituitary; however, the effect on ovarian function is still unknown. The aim of this study was to characterize the expression of SPX and its receptors, galanin receptors 2 and 3 (GALR2/3) in the human ovary and to study its in vitro effect on granulosa cells (GC) function. Follicular fluid (FF) and GC were obtained from normal weigh...
Source: Reproductive Biology - September 2, 2023 Category: Reproduction Medicine Authors: Patrycja Kurowska Monika Dawid Julia Oprocha Natalia Repekta Lo ïse Serra Anthony Estienne Piotr Pawlicki Ma łgorzata Kotula-Balak Fabrice Gu érif Joelle Dupont Agnieszka Rak Source Type: research

Carnosol inhibits KGN cells oxidative stress and apoptosis and attenuates polycystic ovary syndrome phenotypes in mice through Keap1-mediated Nrf2/HO-1 activation
In this study, we established hyperandrogenic PCOS models both in vitro and in vivo. In the human ovarian granulosa cell line, KGN cells, decreased viability and mitochondrial membrane potential, and upregulated reactive oxygen species (ROS) level and apoptosis induced by DHT were partly reversed by carnosol. Western blotting results showed that carnosol treatment inhibited the DHT-activated mitochondrial apoptotic pathway by activating nuclear factor-erythroid 2-related factor (Nrf2)/heme oxygenase 1 (HO-1). Knockdown of Nrf2 by transfecting with siRNA or inhibiting HO-1 by zinc protoporphyrin (ZnPP) blocked the protectiv...
Source: Cell Research - February 14, 2023 Category: Cytology Authors: Rui Ji Fangyuan Jia Xin Chen Yue Gao Jing Yang Source Type: research

SET improved oocyte maturation by serine/threonine protein phosphatase 2A and inhibited oocyte apoptosis in mouse oocytes
Reprod Biol. 2022 Jun 18;22(3):100668. doi: 10.1016/j.repbio.2022.100668. Online ahead of print.ABSTRACTSET is a multifunctional protein involved in a variety of molecular processes such as cell apoptosis and cell-cycle regulation. In ovaries SET is predominantly expressed in theca cells and oocytes. In polycystic ovary syndrome (PCOS) patients the expression of SET was increased than healthy people. The current study was designed to determine whether SET plays a role in oocyte maturation and apoptosis, which may provide clues for the underlying pathological mechanism of follicular development in PCOS patients. Oocytes at ...
Source: Reproductive Biology - June 21, 2022 Category: Reproduction Medicine Authors: Lingling Gao Siying Wang Jianbo Xu Dan Lu Yugui Cui Source Type: research

Growth differentiation factor-11 downregulates steroidogenic acute regulatory protein expression through ALK5-mediated SMAD3 signaling pathway in human granulosa-lutein cells
CONCLUSIONS: This study increases the understanding of the biological function of GDF-11 and provides important insights into the regulation of ovarian steroidogenesis.PMID:35183204 | DOI:10.1186/s12958-022-00912-7
Source: Reproductive Biology - February 20, 2022 Category: Reproduction Medicine Authors: Qiongqiong Jia Boqun Liu Xuan Dang Yanjie Guo Xiaoyu Han Tinglin Song Jung-Chien Cheng Lanlan Fang Source Type: research

Pituitary P62 deficiency leads to female infertility by impairing luteinizing hormone production
Exp Mol Med. 2021 Aug 27. doi: 10.1038/s12276-021-00661-4. Online ahead of print.ABSTRACTP62 is a protein adaptor for various metabolic processes. Mice that lack p62 develop adult-onset obesity. However, investigations on p62 in reproductive dysfunction are rare. In the present study, we explored the effect of p62 on the reproductive system. P62 deficiency-induced reproductive dysfunction occurred at a young age (8 week old). Young systemic p62 knockout (p62-/-) and pituitary-specific p62 knockout (p62flox/flox αGSUcre) mice both presented a normal metabolic state, whereas they displayed infertility phenotypes (attenuated...
Source: Molecular Medicine - August 28, 2021 Category: Molecular Biology Authors: Xing Li Ling Zhou Guiliang Peng Mingyu Liao Linlin Zhang Hua Hu Ling Long Xuefeng Tang Hua Qu Jiaqing Shao Hongting Zheng Min Long Source Type: research

Effect of rNA interference on Oatp3a1 gene expression on biological characteristics and immune factors of ovarian granulosa cells in rats with PCOS.
Authors: Yi W, Li X, Chen K, Li J, Chen K, Pan A Abstract Polycystic ovary syndrome (PCOS) is a common endocrinal metabolic disease, and its pathogenesis has not yet been thoroughly studied. The purpose of this experiment was to investigate the effect of RNA interference on Oatp3a1 gene expression on the biological viability and immune factors of ovarian granulosa cells in rats with PCOS. First, rats were intragastrically administered 1 mg/kg letrozole to successfully construct PCOS model. Western blot, qRT-PCR, CCK8 and flow cytometry were used to detect the gene expression, immune factor protein expression, cell ...
Source: American Journal of Translational Research - September 13, 2020 Category: Research Tags: Am J Transl Res Source Type: research

Melatonin ameliorates excessive PINK1/Parkin-mediated mitophagy by enhancing SIRT1 expression in granulosa cells of PCOS.
In conclusion, melatonin protects against mitochondrial injury in granulosa cells of PCOS by enhancing SIRT1 expression to inhibit excessive PINK1/Parkin-mediated mitophagy. PMID: 32343612 [PubMed - as supplied by publisher]
Source: American Journal of Physiology. Endocrinology and Metabolism - April 27, 2020 Category: Physiology Authors: Yi S, Zheng B, Zhu Y, Cai Y, Sun H, Zhou J Tags: Am J Physiol Endocrinol Metab Source Type: research

Loss of hsa_circ_0118530 inhibits human granulosa-like tumor cell line KGN cell injury by sponging miR-136.
In conclusion, knockdown of hsa_circ_0118530 repressed PCOS progression via sponging miR-136. PMID: 32220601 [PubMed - as supplied by publisher]
Source: Gene - March 23, 2020 Category: Genetics & Stem Cells Authors: Jia C, Wang Y, Yin C, Liu L, Zhou L, Ma Y Tags: Gene Source Type: research

LncRNA HCP5 promotes cell proliferation and inhibits apoptosis via miR-27a-3p/IGF-1 axis in human granulosa-like tumor cell line KGN
This study aimed to reveal the potential roles of long non-coding RNA HCP5 (lncRNA HCP5) and its potential molecular mechanism in polycystic ovarian syndrome (PCOS). The human granulosa-like tumor cell line KGN was used for assessing the effects of HCP5 in the proliferation and apoptosis of granulosa cells (GCs). The results showed that downregulation of HCP5 suppressed cell proliferation through arresting cell cycle progression at G1 phase, and induced the apoptosis via activating mitochondrial pathway, while overexpression of HCP5 played the opposite effects in KGN cells. We predicted and confirmed miR-27a-3p was a direc...
Source: Molecular and Cellular Endocrinology - December 29, 2019 Category: Endocrinology Source Type: research

Oxidative stress increases the 17,20-lyase-catalyzing activity of adrenal P450c17 through p38α in the development of hyperandrogenism
Publication date: Available online 23 January 2019Source: Molecular and Cellular EndocrinologyAuthor(s): Wenjiao Zhu, Bing Han, Mengxia Fan, Nan Wang, Hao Wang, Hui Zhu, Tong Cheng, Shuangxia Zhao, Huaidong Song, Jie QiaoAbstractUnexplained hyperandrogenic oligoanovulation is a main feature of polycystic ovary syndrome (PCOS). P450c17 phosphorylation selectively increases 17,20-lyase activity and androgen biosynthesis but minimally affects 17α-hydroxylase. Studies have recently identified mitogen-activated protein kinase 14 (MAPK14, p38α) as the kinase responsible for enhancing 17,20-lyase activity through P450c17 phosph...
Source: Molecular and Cellular Endocrinology - January 23, 2019 Category: Endocrinology Source Type: research

The role of MiR-324-3p in polycystic ovary syndrome (PCOS) via targeting WNT2B.
CONCLUSIONS: The expression of miR-324-3p in the ovary of PCOS rats is decreased significantly. Overexpression of miR-324-3p can reduce the proliferation and induce the apoptosis of granulosa cells via targeting of WNT2B. PMID: 29917177 [PubMed - in process]
Source: European Review for Medical and Pharmacological Sciences - June 20, 2018 Category: Drugs & Pharmacology Tags: Eur Rev Med Pharmacol Sci Source Type: research