Wa-VP4* as a candidate rotavirus vaccine induced homologous and heterologous virus neutralizing antibody responses in mice, pigs, and cynomolgus monkeys
Vaccine. 2024 Apr 25:S0264-410X(24)00483-3. doi: 10.1016/j.vaccine.2024.04.056. Online ahead of print.ABSTRACTGroup A rotavirus (RVA) is the primary etiological agent of acute gastroenteritis (AGE) in children under 5 years of age. Despite the global implementation of vaccines, rotavirus infections continue to cause over 120,000 deaths annually, with a majority occurring in developing nations. Among infants, the P[8] rotavirus strain is the most prevalent and can be categorized into four distinct lineages. In this investigation, we expressed five VP4(aa26-476) proteins from different P[8] lineages of human rotavirus in E. ...
Source: Vaccine - April 26, 2024 Category: Allergy & Immunology Authors: Guoxing Luo Yuanjun Zeng Roufang Sheng Zhishan Zhang Cao Li Han Yang Yaling Chen Feibo Song Shiyin Zhang Tingdong Li Shengxiang Ge Jun Zhang Ningshao Xia Source Type: research
Wa-VP4* as a candidate rotavirus vaccine induced homologous and heterologous virus neutralizing antibody responses in mice, pigs, and cynomolgus monkeys
Vaccine. 2024 Apr 25:S0264-410X(24)00483-3. doi: 10.1016/j.vaccine.2024.04.056. Online ahead of print.ABSTRACTGroup A rotavirus (RVA) is the primary etiological agent of acute gastroenteritis (AGE) in children under 5 years of age. Despite the global implementation of vaccines, rotavirus infections continue to cause over 120,000 deaths annually, with a majority occurring in developing nations. Among infants, the P[8] rotavirus strain is the most prevalent and can be categorized into four distinct lineages. In this investigation, we expressed five VP4(aa26-476) proteins from different P[8] lineages of human rotavirus in E. ...
Source: Vaccine - April 26, 2024 Category: Allergy & Immunology Authors: Guoxing Luo Yuanjun Zeng Roufang Sheng Zhishan Zhang Cao Li Han Yang Yaling Chen Feibo Song Shiyin Zhang Tingdong Li Shengxiang Ge Jun Zhang Ningshao Xia Source Type: research
Viruses, Vol. 16, Pages 660: Histo-Blood Group Antigen-Producing Bacterial Cocktail Reduces Rotavirus A, B, and C Infection and Disease in Gnotobiotic Piglets
In this study, germ-free piglets were colonized with HBGA+ or HBGA- bacterial cocktail and infected with RVA/RVB/RVC of different genotypes. Diarrhea severity, virus shedding, immunoglobulin A (IgA) Ab titers, and cytokine levels were evaluated. Overall, colonization with HBGA+ bacteria resulted in reduced diarrhea severity and virus shedding compared to the HBGA- bacteria. Consistent with our hypothesis, the reduced severity of RV disease and infection was not associated with significant alterations in immune responses. Additionally, colonization with HBGA+ bacteria conferred beneficial effects irrespective of the piglet ...
Source: Viruses - April 24, 2024 Category: Virology Authors: Sergei A. Raev Maryssa K. Kick Maria Chellis Joshua O. Amimo Linda J. Saif Anastasia N. Vlasova Tags: Communication Source Type: research
Evaluation of different genomic regions of rotavirus B and rotavirus C for development of real-time RT ‒PCR assays
The causative agents of diarrhea, rotavirus B (RVB) and rotavirus C (RVC) are common in adults and patients of all age groups, respectively. Due to the Rotavirus A (RVA) vaccination program, a significant decr... (Source: Virology Journal)
Source: Virology Journal - April 24, 2024 Category: Virology Authors: Madhuri S. Joshi, Manohar S. Shinde and Mallika Lavania Tags: Methodology Source Type: research
Effect of Non-Rotavirus Enteric Infections on Vaccine Efficacy in a ROTASIIL Clinical Trial
This study examined the relative proportion of enteric pathogens associated with severe gastroenteritis (GE) among children younger than 2 years in a phase III efficacy trial of the ROTASIIL® vaccine in India, evaluated the impact of co-infections on vaccine efficacy (VE), and characterized the association between specific pathogens and the clinical profile of severe GE. Stored stool samples collected from cases of severe GE in the phase III trial were tested by quantitative polymerase chain reaction using TaqMan™ Array Cards. Etiology was attributed by calculating the adjusted attributable fraction (AF) for each pathog...
Source: The American Journal of Tropical Medicine and Hygiene - April 16, 2024 Category: Tropical Medicine Authors: Dilip Abraham Prasanna Samuel Premkumar James A Platts-Mills Tushar Tewari Niranjan Bhat Revathi Rajendiran Hemavathi Gunalan Gagandeep Kang Source Type: research
Effect of Non-Rotavirus Enteric Infections on Vaccine Efficacy in a ROTASIIL Clinical Trial
This study examined the relative proportion of enteric pathogens associated with severe gastroenteritis (GE) among children younger than 2 years in a phase III efficacy trial of the ROTASIIL® vaccine in India, evaluated the impact of co-infections on vaccine efficacy (VE), and characterized the association between specific pathogens and the clinical profile of severe GE. Stored stool samples collected from cases of severe GE in the phase III trial were tested by quantitative polymerase chain reaction using TaqMan™ Array Cards. Etiology was attributed by calculating the adjusted attributable fraction (AF) for each pathog...
Source: Am J Trop Med Hyg - April 16, 2024 Category: Infectious Diseases Authors: Dilip Abraham Prasanna Samuel Premkumar James A Platts-Mills Tushar Tewari Niranjan Bhat Revathi Rajendiran Hemavathi Gunalan Gagandeep Kang Source Type: research
Effect of Non-Rotavirus Enteric Infections on Vaccine Efficacy in a ROTASIIL Clinical Trial
This study examined the relative proportion of enteric pathogens associated with severe gastroenteritis (GE) among children younger than 2 years in a phase III efficacy trial of the ROTASIIL® vaccine in India, evaluated the impact of co-infections on vaccine efficacy (VE), and characterized the association between specific pathogens and the clinical profile of severe GE. Stored stool samples collected from cases of severe GE in the phase III trial were tested by quantitative polymerase chain reaction using TaqMan™ Array Cards. Etiology was attributed by calculating the adjusted attributable fraction (AF) for each pathog...
Source: The American Journal of Tropical Medicine and Hygiene - April 16, 2024 Category: Tropical Medicine Authors: Dilip Abraham Prasanna Samuel Premkumar James A Platts-Mills Tushar Tewari Niranjan Bhat Revathi Rajendiran Hemavathi Gunalan Gagandeep Kang Source Type: research
Effect of Non-Rotavirus Enteric Infections on Vaccine Efficacy in a ROTASIIL Clinical Trial
This study examined the relative proportion of enteric pathogens associated with severe gastroenteritis (GE) among children younger than 2 years in a phase III efficacy trial of the ROTASIIL® vaccine in India, evaluated the impact of co-infections on vaccine efficacy (VE), and characterized the association between specific pathogens and the clinical profile of severe GE. Stored stool samples collected from cases of severe GE in the phase III trial were tested by quantitative polymerase chain reaction using TaqMan™ Array Cards. Etiology was attributed by calculating the adjusted attributable fraction (AF) for each pathog...
Source: Am J Trop Med Hyg - April 16, 2024 Category: Infectious Diseases Authors: Dilip Abraham Prasanna Samuel Premkumar James A Platts-Mills Tushar Tewari Niranjan Bhat Revathi Rajendiran Hemavathi Gunalan Gagandeep Kang Source Type: research
Effect of Non-Rotavirus Enteric Infections on Vaccine Efficacy in a ROTASIIL Clinical Trial
This study examined the relative proportion of enteric pathogens associated with severe gastroenteritis (GE) among children younger than 2 years in a phase III efficacy trial of the ROTASIIL® vaccine in India, evaluated the impact of co-infections on vaccine efficacy (VE), and characterized the association between specific pathogens and the clinical profile of severe GE. Stored stool samples collected from cases of severe GE in the phase III trial were tested by quantitative polymerase chain reaction using TaqMan™ Array Cards. Etiology was attributed by calculating the adjusted attributable fraction (AF) for each pathog...
Source: The American Journal of Tropical Medicine and Hygiene - April 16, 2024 Category: Tropical Medicine Authors: Dilip Abraham Prasanna Samuel Premkumar James A Platts-Mills Tushar Tewari Niranjan Bhat Revathi Rajendiran Hemavathi Gunalan Gagandeep Kang Source Type: research
Effect of Non-Rotavirus Enteric Infections on Vaccine Efficacy in a ROTASIIL Clinical Trial
This study examined the relative proportion of enteric pathogens associated with severe gastroenteritis (GE) among children younger than 2 years in a phase III efficacy trial of the ROTASIIL® vaccine in India, evaluated the impact of co-infections on vaccine efficacy (VE), and characterized the association between specific pathogens and the clinical profile of severe GE. Stored stool samples collected from cases of severe GE in the phase III trial were tested by quantitative polymerase chain reaction using TaqMan™ Array Cards. Etiology was attributed by calculating the adjusted attributable fraction (AF) for each pathog...
Source: Am J Trop Med Hyg - April 16, 2024 Category: Infectious Diseases Authors: Dilip Abraham Prasanna Samuel Premkumar James A Platts-Mills Tushar Tewari Niranjan Bhat Revathi Rajendiran Hemavathi Gunalan Gagandeep Kang Source Type: research
Lactiplantibacillus plantarum surface-displayed VP6 (PoRV) protein can prevent PoRV infection in piglets
Int Immunopharmacol. 2024 Apr 13;133:112079. doi: 10.1016/j.intimp.2024.112079. Online ahead of print.ABSTRACTPorcine rotavirus (PoRV) poses a threat to the development of animal husbandry and human health, leading to substantial economic losses. VP6 protein is the most abundant component in virus particles and also the core structural protein of the virus. Firstly, this study developed an antibiotic-resistance-free, environmentally friendly expression vector, named asd-araC-PBAD-alr (AAPA). Then Recombinant Lactiplantibacillus plantarum (L. plantarum) strains induced by arabinose to express VP6 and VP6-pFc fusion proteins...
Source: International Immunopharmacology - April 14, 2024 Category: Allergy & Immunology Authors: Junhong Wang Haixu Wang Dongliang Zhang Fangyuan Liu Xiaoxu Li Ming Gao Mingyang Cheng Hongyu Bao Jiaxing Zhan Yan Zeng Chunfeng Wang Xin Cao Source Type: research
Lactiplantibacillus plantarum surface-displayed VP6 (PoRV) protein can prevent PoRV infection in piglets
Int Immunopharmacol. 2024 Apr 13;133:112079. doi: 10.1016/j.intimp.2024.112079. Online ahead of print.ABSTRACTPorcine rotavirus (PoRV) poses a threat to the development of animal husbandry and human health, leading to substantial economic losses. VP6 protein is the most abundant component in virus particles and also the core structural protein of the virus. Firstly, this study developed an antibiotic-resistance-free, environmentally friendly expression vector, named asd-araC-PBAD-alr (AAPA). Then Recombinant Lactiplantibacillus plantarum (L. plantarum) strains induced by arabinose to express VP6 and VP6-pFc fusion proteins...
Source: International Immunopharmacology - April 14, 2024 Category: Allergy & Immunology Authors: Junhong Wang Haixu Wang Dongliang Zhang Fangyuan Liu Xiaoxu Li Ming Gao Mingyang Cheng Hongyu Bao Jiaxing Zhan Yan Zeng Chunfeng Wang Xin Cao Source Type: research
Etiology of diarrheal hospitalizations following rotavirus vaccine implementation and association of enteric pathogens with malnutrition among under-five children in India
AbstractMalnourished children are at higher risk of mortality and morbidity following diarrheal illness and certain enteropathogens have been associated with malnutrition in children. Very few studies have comprehensively looked at the etiology of diarrhea in malnourished children and most have used conventional diagnostic methods with suboptimal sensitivity. We used a highly sensitive molecular approach against a broad range of pathogens causing diarrhea and examined their association with malnutrition. In addition, we looked at the pathogen diversity of pediatric diarrhea, three years after the nationwide rotavirus vacci...
Source: Gut Pathogens - April 10, 2024 Category: Microbiology Source Type: research
Spectroscopic Analysis of the Bacterially Expressed Head Domain of Rotavirus VP6
Biosci Rep. 2024 Apr 9:BSR20232178. doi: 10.1042/BSR20232178. Online ahead of print.ABSTRACTThe rotavirus capsid protein VP6 forms the middle of three protein layers and is responsible for many critical steps in the viral life cycle. VP6 as a structural protein can be used in various applications including as a subunit vaccine component. The head domain of VP6 (VP6H) contains key sequences that allow the protein to trimerize and that represent epitopes that are recognized by human antibodies in the viral particle. The domain is rich in beta-sheet secondary structures. Here VP6H was solubilised from bacterial inclusion bodi...
Source: Bioscience Reports - April 9, 2024 Category: Biomedical Science Authors: Milaan Simone Strachan Tshepo Mashapa Samantha Gildenhuys Source Type: research
Spectroscopic Analysis of the Bacterially Expressed Head Domain of Rotavirus VP6
Biosci Rep. 2024 Apr 9:BSR20232178. doi: 10.1042/BSR20232178. Online ahead of print.ABSTRACTThe rotavirus capsid protein VP6 forms the middle of three protein layers and is responsible for many critical steps in the viral life cycle. VP6 as a structural protein can be used in various applications including as a subunit vaccine component. The head domain of VP6 (VP6H) contains key sequences that allow the protein to trimerize and that represent epitopes that are recognized by human antibodies in the viral particle. The domain is rich in beta-sheet secondary structures. Here VP6H was solubilised from bacterial inclusion bodi...
Source: Bioscience Reports - April 9, 2024 Category: Biomedical Science Authors: Milaan Simone Strachan Tshepo Mashapa Samantha Gildenhuys Source Type: research
