Survival of O157 and non-O157 shiga toxin-producing Escherichia coli in Korean style kimchi
Food Microbiol. 2024 Aug;121:104526. doi: 10.1016/j.fm.2024.104526. Epub 2024 Mar 30.ABSTRACTKorean style kimchi contaminated with Shiga toxin-producing Escherichia coli (STEC) O157:H7 was the cause of an outbreak in Canada from December 2021 to January 2022. To determine if this STEC O157:H7 has greater potential for survival in kimchi than other STEC, the outbreak strain and six other STEC strains (O26:H11, O91:H21, O103:H2, O121:H19, and two O157:H7) were inoculated individually at 6 to 6.5 log CFU/g into commercially sourced kimchi and incubation at 4 °C. At intervals of seven days inoculated and control kimchi was pl...
Source: Food Microbiology - April 18, 2024 Category: Food Science Authors: Alexander Gill Tanis McMahon Christina Ferrato Linda Chui Source Type: research

Survival of O157 and non-O157 shiga toxin-producing Escherichia coli in Korean style kimchi
Food Microbiol. 2024 Aug;121:104526. doi: 10.1016/j.fm.2024.104526. Epub 2024 Mar 30.ABSTRACTKorean style kimchi contaminated with Shiga toxin-producing Escherichia coli (STEC) O157:H7 was the cause of an outbreak in Canada from December 2021 to January 2022. To determine if this STEC O157:H7 has greater potential for survival in kimchi than other STEC, the outbreak strain and six other STEC strains (O26:H11, O91:H21, O103:H2, O121:H19, and two O157:H7) were inoculated individually at 6 to 6.5 log CFU/g into commercially sourced kimchi and incubation at 4 °C. At intervals of seven days inoculated and control kimchi was pl...
Source: Food Microbiology - April 18, 2024 Category: Food Science Authors: Alexander Gill Tanis McMahon Christina Ferrato Linda Chui Source Type: research

Survival of O157 and non-O157 shiga toxin-producing Escherichia coli in Korean style kimchi
Food Microbiol. 2024 Aug;121:104526. doi: 10.1016/j.fm.2024.104526. Epub 2024 Mar 30.ABSTRACTKorean style kimchi contaminated with Shiga toxin-producing Escherichia coli (STEC) O157:H7 was the cause of an outbreak in Canada from December 2021 to January 2022. To determine if this STEC O157:H7 has greater potential for survival in kimchi than other STEC, the outbreak strain and six other STEC strains (O26:H11, O91:H21, O103:H2, O121:H19, and two O157:H7) were inoculated individually at 6 to 6.5 log CFU/g into commercially sourced kimchi and incubation at 4 °C. At intervals of seven days inoculated and control kimchi was pl...
Source: Food Microbiology - April 18, 2024 Category: Food Science Authors: Alexander Gill Tanis McMahon Christina Ferrato Linda Chui Source Type: research

Survival of O157 and non-O157 shiga toxin-producing Escherichia coli in Korean style kimchi
Food Microbiol. 2024 Aug;121:104526. doi: 10.1016/j.fm.2024.104526. Epub 2024 Mar 30.ABSTRACTKorean style kimchi contaminated with Shiga toxin-producing Escherichia coli (STEC) O157:H7 was the cause of an outbreak in Canada from December 2021 to January 2022. To determine if this STEC O157:H7 has greater potential for survival in kimchi than other STEC, the outbreak strain and six other STEC strains (O26:H11, O91:H21, O103:H2, O121:H19, and two O157:H7) were inoculated individually at 6 to 6.5 log CFU/g into commercially sourced kimchi and incubation at 4 °C. At intervals of seven days inoculated and control kimchi was pl...
Source: Food Microbiology - April 18, 2024 Category: Food Science Authors: Alexander Gill Tanis McMahon Christina Ferrato Linda Chui Source Type: research

Survival of O157 and non-O157 shiga toxin-producing Escherichia coli in Korean style kimchi
Food Microbiol. 2024 Aug;121:104526. doi: 10.1016/j.fm.2024.104526. Epub 2024 Mar 30.ABSTRACTKorean style kimchi contaminated with Shiga toxin-producing Escherichia coli (STEC) O157:H7 was the cause of an outbreak in Canada from December 2021 to January 2022. To determine if this STEC O157:H7 has greater potential for survival in kimchi than other STEC, the outbreak strain and six other STEC strains (O26:H11, O91:H21, O103:H2, O121:H19, and two O157:H7) were inoculated individually at 6 to 6.5 log CFU/g into commercially sourced kimchi and incubation at 4 °C. At intervals of seven days inoculated and control kimchi was pl...
Source: Food Microbiology - April 18, 2024 Category: Food Science Authors: Alexander Gill Tanis McMahon Christina Ferrato Linda Chui Source Type: research

Survival of O157 and non-O157 shiga toxin-producing Escherichia coli in Korean style kimchi
Food Microbiol. 2024 Aug;121:104526. doi: 10.1016/j.fm.2024.104526. Epub 2024 Mar 30.ABSTRACTKorean style kimchi contaminated with Shiga toxin-producing Escherichia coli (STEC) O157:H7 was the cause of an outbreak in Canada from December 2021 to January 2022. To determine if this STEC O157:H7 has greater potential for survival in kimchi than other STEC, the outbreak strain and six other STEC strains (O26:H11, O91:H21, O103:H2, O121:H19, and two O157:H7) were inoculated individually at 6 to 6.5 log CFU/g into commercially sourced kimchi and incubation at 4 °C. At intervals of seven days inoculated and control kimchi was pl...
Source: Food Microbiology - April 18, 2024 Category: Food Science Authors: Alexander Gill Tanis McMahon Christina Ferrato Linda Chui Source Type: research

Survival of O157 and non-O157 shiga toxin-producing Escherichia coli in Korean style kimchi
Food Microbiol. 2024 Aug;121:104526. doi: 10.1016/j.fm.2024.104526. Epub 2024 Mar 30.ABSTRACTKorean style kimchi contaminated with Shiga toxin-producing Escherichia coli (STEC) O157:H7 was the cause of an outbreak in Canada from December 2021 to January 2022. To determine if this STEC O157:H7 has greater potential for survival in kimchi than other STEC, the outbreak strain and six other STEC strains (O26:H11, O91:H21, O103:H2, O121:H19, and two O157:H7) were inoculated individually at 6 to 6.5 log CFU/g into commercially sourced kimchi and incubation at 4 °C. At intervals of seven days inoculated and control kimchi was pl...
Source: Food Microbiology - April 18, 2024 Category: Food Science Authors: Alexander Gill Tanis McMahon Christina Ferrato Linda Chui Source Type: research

Survival of O157 and non-O157 shiga toxin-producing Escherichia coli in Korean style kimchi
Food Microbiol. 2024 Aug;121:104526. doi: 10.1016/j.fm.2024.104526. Epub 2024 Mar 30.ABSTRACTKorean style kimchi contaminated with Shiga toxin-producing Escherichia coli (STEC) O157:H7 was the cause of an outbreak in Canada from December 2021 to January 2022. To determine if this STEC O157:H7 has greater potential for survival in kimchi than other STEC, the outbreak strain and six other STEC strains (O26:H11, O91:H21, O103:H2, O121:H19, and two O157:H7) were inoculated individually at 6 to 6.5 log CFU/g into commercially sourced kimchi and incubation at 4 °C. At intervals of seven days inoculated and control kimchi was pl...
Source: Food Microbiology - April 18, 2024 Category: Food Science Authors: Alexander Gill Tanis McMahon Christina Ferrato Linda Chui Source Type: research

A rapid and sensitive one-pot platform integrating fluorogenic RNA aptamers and CRISPR-Cas13a for visual detection of monkeypox virus
In this study, we utilized a cost-effective and stable fluorogenic RNA aptamer (Mango III), specifically binding and illuminating the fluorophore TO3-3 PEG-Biotin Fluorophore (TO3), as a reporter for Cas13a trans-cleavage activity. We propose a comprehensive strategy integrating RNA aptamer, recombinase-aided amplification (RAA), and CRISPR-Cas13a systems for the molecular detection of MPXV target. Leveraging the inherent collateral cleavage properties of the Cas13a system, we established high-sensitivity and specificity assays to distinguish MPXV from other Orthopoxviruses (OPVs). A streamlined one-pot protocol was develo...
Source: Biosensors and Bioelectronics - April 18, 2024 Category: Biotechnology Authors: Xiao Wang Xiaobao Deng Yidun Zhang Weiyi Dong Qiao Rao Qingmei Huang Fei Tang Rong Shen Hongzhi Xu Zhen Jin Youzhi Tang Dan Du Source Type: research

Shifting spatial, temporal and demographic patterns of dengue incidence and associated meteorological factors in Jazan Region of Saudi Arabia from 2015-2020
CONCLUSION: Enhanced surveillance, outbreak forecasting, targeted control activities, and integrated prevention policies grounded in continuous evidence assessment can effectively address endemic dengue transmission in Jazan. This study provides key insights to optimize data-driven decision-making for dengue control in Saudi Arabia.PMID:38634364 | DOI:10.4103/JVBD.JVBD_15_24 (Source: Journal of Vector Borne Diseases)
Source: Journal of Vector Borne Diseases - April 18, 2024 Category: Infectious Diseases Authors: Ahmad Y Alqassim Mohammed Badedi Mohammed A Muaddi Abdullah A Alharbi Mohammad A Jareebi Anwar M Makeen Maged El-Setouhy Osama B Albasheer Abdullah Sabai Ahmed Sahly Source Type: research

Potential application of mesenchymal stromal cells as a new therapeutic approach in acute respiratory distress syndrome and pulmonary fibrosis
Respir Res. 2024 Apr 18;25(1):170. doi: 10.1186/s12931-024-02795-1.ABSTRACTWhile the COVID-19 outbreak and its complications are still under investigation, post-inflammatory pulmonary fibrosis (PF) has already been described as a long-term sequela of acute respiratory distress syndrome (ARDS) secondary to SARS-CoV2 infection. However, therapeutical strategies for patients with ARDS and PF are still limited and do not significantly extend lifespan. So far, lung transplantation remains the only definitive treatment for end-stage PF. Over the last years, numerous preclinical and clinical studies have shown that allogeneic mes...
Source: Cell Research - April 18, 2024 Category: Cytology Authors: Giulia Gazzaniga Marta Voltini Alessandro Carletti Elisa Lenta Federica Meloni Domenica Federica Briganti Maria Antonietta Avanzini Patrizia Comoli Mirko Belliato Source Type: research

A rapid and sensitive one-pot platform integrating fluorogenic RNA aptamers and CRISPR-Cas13a for visual detection of monkeypox virus
In this study, we utilized a cost-effective and stable fluorogenic RNA aptamer (Mango III), specifically binding and illuminating the fluorophore TO3-3 PEG-Biotin Fluorophore (TO3), as a reporter for Cas13a trans-cleavage activity. We propose a comprehensive strategy integrating RNA aptamer, recombinase-aided amplification (RAA), and CRISPR-Cas13a systems for the molecular detection of MPXV target. Leveraging the inherent collateral cleavage properties of the Cas13a system, we established high-sensitivity and specificity assays to distinguish MPXV from other Orthopoxviruses (OPVs). A streamlined one-pot protocol was develo...
Source: Biosensors and Bioelectronics - April 18, 2024 Category: Biotechnology Authors: Xiao Wang Xiaobao Deng Yidun Zhang Weiyi Dong Qiao Rao Qingmei Huang Fei Tang Rong Shen Hongzhi Xu Zhen Jin Youzhi Tang Dan Du Source Type: research

Survival of O157 and non-O157 shiga toxin-producing Escherichia coli in Korean style kimchi
Food Microbiol. 2024 Aug;121:104526. doi: 10.1016/j.fm.2024.104526. Epub 2024 Mar 30.ABSTRACTKorean style kimchi contaminated with Shiga toxin-producing Escherichia coli (STEC) O157:H7 was the cause of an outbreak in Canada from December 2021 to January 2022. To determine if this STEC O157:H7 has greater potential for survival in kimchi than other STEC, the outbreak strain and six other STEC strains (O26:H11, O91:H21, O103:H2, O121:H19, and two O157:H7) were inoculated individually at 6 to 6.5 log CFU/g into commercially sourced kimchi and incubation at 4 °C. At intervals of seven days inoculated and control kimchi was pl...
Source: Food Microbiology - April 18, 2024 Category: Food Science Authors: Alexander Gill Tanis McMahon Christina Ferrato Linda Chui Source Type: research

Shifting spatial, temporal and demographic patterns of dengue incidence and associated meteorological factors in Jazan Region of Saudi Arabia from 2015-2020
CONCLUSION: Enhanced surveillance, outbreak forecasting, targeted control activities, and integrated prevention policies grounded in continuous evidence assessment can effectively address endemic dengue transmission in Jazan. This study provides key insights to optimize data-driven decision-making for dengue control in Saudi Arabia.PMID:38634364 | DOI:10.4103/JVBD.JVBD_15_24 (Source: Journal of Vector Borne Diseases)
Source: Journal of Vector Borne Diseases - April 18, 2024 Category: Infectious Diseases Authors: Ahmad Y Alqassim Mohammed Badedi Mohammed A Muaddi Abdullah A Alharbi Mohammad A Jareebi Anwar M Makeen Maged El-Setouhy Osama B Albasheer Abdullah Sabai Ahmed Sahly Source Type: research

Strong resistance to β‐cyfluthrin in a strain of the beetle Alphitobius diaperinus: a de novo transcriptome analysis
An RNA-Seq approach, enhanced by biochemical analyses, established that one of the main reasons for the high resistance to β-cyfluthrin in anAlphitobius diaperinus strain comes from high basal activities for detoxification enzymes. AbstractThe lesser mealworm,Alphitobius diaperinus, is an invasive tenebrionid beetle and a vector of pathogens. Due to the emergence of insecticide resistance and consequent outbreaks that generate significant phytosanitary and energy costs for poultry farmers, it has become a major insect pest worldwide. To better understand the molecular mechanisms behind this resistance, we studied a strain...
Source: Insect Science - April 18, 2024 Category: Biology Authors: Gwenola Gouesbet, David Renault, St éphane A. P. Derocles, Hervé Colinet Tags: ORIGINAL ARTICLE Source Type: research