Strong resistance to β‐cyfluthrin in a strain of the beetle Alphitobius diaperinus: a de novo transcriptome analysis

An RNA-Seq approach, enhanced by biochemical analyses, established that one of the main reasons for the high resistance to β-cyfluthrin in anAlphitobius diaperinus strain comes from high basal activities for detoxification enzymes. AbstractThe lesser mealworm,Alphitobius diaperinus, is an invasive tenebrionid beetle and a vector of pathogens. Due to the emergence of insecticide resistance and consequent outbreaks that generate significant phytosanitary and energy costs for poultry farmers, it has become a major insect pest worldwide. To better understand the molecular mechanisms behind this resistance, we studied a strain ofA. diaperinus from a poultry house in Brittany that was found to be highly resistant to theβ-cyfluthrin. The strain survivedβ-cyfluthrin exposures corresponding to more than 100 times the recommended dose. We used a comparativede novo RNA-Seq approach to explore genes expression in resistant versus sensitive strains. Ourde novo transcriptomic analyses showed that responses toβ-cyfluthrin likely involved a whole set of resistance mechanisms. Genes related to detoxification, metabolic resistance, cuticular hydrocarbon biosynthesis and proteolysis were found to be constitutively overexpressed in the resistant compared to the sensitive strain. Follow-up enzymatic assays confirmed that the resistant strain exhibited high basal activities for detoxification enzymes such as cytochrome P450 monooxygenase and glutathione-S-transferase. The in-depth analysis of ...
Source: Insect Science - Category: Biology Authors: Tags: ORIGINAL ARTICLE Source Type: research