A novel metagenomic approach uncovers phage genes as markers for increased disinfectant tolerance in mixed Listeria monocytogenes communities
In this study, we applied a novel metagenomic approach to detect genes associated with disinfectant tolerance in mixed L. monocytogenes planktonic communities. Two communities, consisting of 71 and 80 isolates each, were treated with the food industry disinfectants benzalkonium chloride (BC, 1.75 mg/L) or peracetic acid (PAA, 38 mg/L). The communities were subjected to metagenomic sequencing and differences in individual gene abundances between biocide-free control communities and biocide-treated communities were determined. A significant increase in the abundance of Listeria phage-associated genes was observed in both com...
Source: Infection, Genetics and Evolution - March 11, 2024 Category: Genetics & Stem Cells Authors: Agnete Kirstine Karlsmose Mirena Ivanova Martin Laage Kragh Jette Sejer Kjeldgaard Saria Otani Christina Aaby Svendsen Bojan Papi ć Irena Zdovc Taurai Tasara Roger Stephan Even Heir Solveig Langsrud Trond M øretrø Paw Dalgaard Annette Fagerlund Lisbeth Source Type: research
A novel metagenomic approach uncovers phage genes as markers for increased disinfectant tolerance in mixed Listeria monocytogenes communities
In this study, we applied a novel metagenomic approach to detect genes associated with disinfectant tolerance in mixed L. monocytogenes planktonic communities. Two communities, consisting of 71 and 80 isolates each, were treated with the food industry disinfectants benzalkonium chloride (BC, 1.75 mg/L) or peracetic acid (PAA, 38 mg/L). The communities were subjected to metagenomic sequencing and differences in individual gene abundances between biocide-free control communities and biocide-treated communities were determined. A significant increase in the abundance of Listeria phage-associated genes was observed in both com...
Source: Infection, Genetics and Evolution - March 11, 2024 Category: Genetics & Stem Cells Authors: Agnete Kirstine Karlsmose Mirena Ivanova Martin Laage Kragh Jette Sejer Kjeldgaard Saria Otani Christina Aaby Svendsen Bojan Papi ć Irena Zdovc Taurai Tasara Roger Stephan Even Heir Solveig Langsrud Trond M øretrø Paw Dalgaard Annette Fagerlund Lisbeth Source Type: research
Recurrent, ICD-associated L. monocytogenes bacteraemia with multiple septic pulmonary embolisms over a 2-year period
ConclusionThe confirmation of relapses through cgMLST analysis highlights the persistence ofListeria monocytogenes and the potential for recurrence even after apparent resolution of symptoms in patients with foreign material. It emphasises the necessity for a comprehensive assessment to identify and mitigate the risk of relapses, thereby ensuring optimal management and outcomes. (Source: Infection)
Source: Infection - March 6, 2024 Category: Infectious Diseases Source Type: research
Cross-regulation of Listeria monocytogenes and the host ubiquitin system in listeriosis
Eur J Cell Biol. 2024 Feb 28;103(2):151401. doi: 10.1016/j.ejcb.2024.151401. Online ahead of print.ABSTRACTThe facultative intracellular bacterium Listeria (L.) monocytogenes may cause severe diseases in humans and animals. The control of listeriosis/L. monocytogenes requires the concerted action of cells of the innate and adaptive immune systems. In this regard, cell-intrinsic immunity of infected cells, activated by the immune responses, is crucial for the control and elimination intracellular L. monocytogenes. Both the immune response against L. monocytogenes and cell intrinsic pathogen control are critically regulated ...
Source: European Journal of Cell Biology - March 5, 2024 Category: Cytology Authors: Yuan Zhuang Johanna B Fischer Gopala Nishanth Dirk Schl üter Source Type: research
Cross-regulation of Listeria monocytogenes and the host ubiquitin system in listeriosis
Eur J Cell Biol. 2024 Feb 28;103(2):151401. doi: 10.1016/j.ejcb.2024.151401. Online ahead of print.ABSTRACTThe facultative intracellular bacterium Listeria (L.) monocytogenes may cause severe diseases in humans and animals. The control of listeriosis/L. monocytogenes requires the concerted action of cells of the innate and adaptive immune systems. In this regard, cell-intrinsic immunity of infected cells, activated by the immune responses, is crucial for the control and elimination intracellular L. monocytogenes. Both the immune response against L. monocytogenes and cell intrinsic pathogen control are critically regulated ...
Source: European Journal of Cell Biology - March 5, 2024 Category: Cytology Authors: Yuan Zhuang Johanna B Fischer Gopala Nishanth Dirk Schl üter Source Type: research
Cross-regulation of Listeria monocytogenes and the host ubiquitin system in listeriosis
Eur J Cell Biol. 2024 Feb 28;103(2):151401. doi: 10.1016/j.ejcb.2024.151401. Online ahead of print.ABSTRACTThe facultative intracellular bacterium Listeria (L.) monocytogenes may cause severe diseases in humans and animals. The control of listeriosis/L. monocytogenes requires the concerted action of cells of the innate and adaptive immune systems. In this regard, cell-intrinsic immunity of infected cells, activated by the immune responses, is crucial for the control and elimination intracellular L. monocytogenes. Both the immune response against L. monocytogenes and cell intrinsic pathogen control are critically regulated ...
Source: European Journal of Cell Biology - March 5, 2024 Category: Cytology Authors: Yuan Zhuang Johanna B Fischer Gopala Nishanth Dirk Schl üter Source Type: research
Cross-regulation of Listeria monocytogenes and the host ubiquitin system in listeriosis
Eur J Cell Biol. 2024 Feb 28;103(2):151401. doi: 10.1016/j.ejcb.2024.151401. Online ahead of print.ABSTRACTThe facultative intracellular bacterium Listeria (L.) monocytogenes may cause severe diseases in humans and animals. The control of listeriosis/L. monocytogenes requires the concerted action of cells of the innate and adaptive immune systems. In this regard, cell-intrinsic immunity of infected cells, activated by the immune responses, is crucial for the control and elimination intracellular L. monocytogenes. Both the immune response against L. monocytogenes and cell intrinsic pathogen control are critically regulated ...
Source: European Journal of Cell Biology - March 5, 2024 Category: Cytology Authors: Yuan Zhuang Johanna B Fischer Gopala Nishanth Dirk Schl üter Source Type: research
Cross-regulation of Listeria monocytogenes and the host ubiquitin system in listeriosis
Eur J Cell Biol. 2024 Feb 28;103(2):151401. doi: 10.1016/j.ejcb.2024.151401. Online ahead of print.ABSTRACTThe facultative intracellular bacterium Listeria (L.) monocytogenes may cause severe diseases in humans and animals. The control of listeriosis/L. monocytogenes requires the concerted action of cells of the innate and adaptive immune systems. In this regard, cell-intrinsic immunity of infected cells, activated by the immune responses, is crucial for the control and elimination intracellular L. monocytogenes. Both the immune response against L. monocytogenes and cell intrinsic pathogen control are critically regulated ...
Source: European Journal of Cell Biology - March 5, 2024 Category: Cytology Authors: Yuan Zhuang Johanna B Fischer Gopala Nishanth Dirk Schl üter Source Type: research
Revisiting old friends: updates on the role of two-component signaling systems in Listeria monocytogenes survival and pathogenesis
Infection and Immunity, Ahead of Print. (Source: Infection and Immunity)
Source: Infection and Immunity - March 4, 2024 Category: Infectious Diseases Authors: Xiomarie Alejandro-NavarretoNancy E. Freitag1Department of Microbiology and Immunology, University of Illinois at Chicago, Chicago, Illinois, USA2Department of Pharmaceutical Sciences, University of Illinois at Chicago, Chicago, Illinois, USA Karen M. Ott Source Type: research
A selection process based on the robustness of anti-Listeria monocytogenes activity reveals two strains of Carnobacterium maltaromaticum with biopreservation properties in cheese
In this study, the anti-Listeria monocytogenes EGDelux properties of a collection of 77 Carnobacterium maltaromaticum strains were investigated by high throughput competition assays under varying conditions of co-culture inoculation level, time interval between inoculation with C. maltaromaticum and L. monocytogenes, pH, and NaCl, resulting in 1309 different combinations of C. maltaromaticum strains and culture conditions. This screening led to the selection of two candidate strains with potent and robust anti-L. monocytogenes activities. Deferred growth inhibition assays followed by halo measurements, and liquid co-cultur...
Source: International Journal of Food Microbiology - March 3, 2024 Category: Food Science Authors: Lamia Cherrat Alexis Dijamentiuk Sara M El Kheir C écile Mangavel Annelore Elfassy Anne-Marie Revol-Junelles Fr édéric Borges Source Type: research
Microbiological safety of dry-cured fish from the raw material to the end of processing
In this study total aerobic colony count and Enterobacteriaceae, as proxy of process hygiene criteria, and detection of Listeria monocytogenes and concentration of histamine, as food safety criteria, were investigated in Salmo salar (salmon), Xiphias gladius (swordfish) and Thunnus albacares (yellowfin tuna), before, during, and at the end of a dry-curing process, performed in a dedicated cabinet, at controlled temperature, relative humidity and ventilation, up to 240 h. The microbiological parameters were investigated in the tested fish products by culture methods and shotgun metagenomic, while the presence of histamine, ...
Source: International Journal of Food Microbiology - March 3, 2024 Category: Food Science Authors: Valentina Indio Federica Savini Fausto Gardini Federica Barbieri Laura Prandini Yitagele Terefe Mekonnen Federico Tomasello Federica Giacometti Alessandro Seguino Andrea Serraino Alessandra De Cesare Source Type: research
A selection process based on the robustness of anti-Listeria monocytogenes activity reveals two strains of Carnobacterium maltaromaticum with biopreservation properties in cheese
In this study, the anti-Listeria monocytogenes EGDelux properties of a collection of 77 Carnobacterium maltaromaticum strains were investigated by high throughput competition assays under varying conditions of co-culture inoculation level, time interval between inoculation with C. maltaromaticum and L. monocytogenes, pH, and NaCl, resulting in 1309 different combinations of C. maltaromaticum strains and culture conditions. This screening led to the selection of two candidate strains with potent and robust anti-L. monocytogenes activities. Deferred growth inhibition assays followed by halo measurements, and liquid co-cultur...
Source: International Journal of Food Microbiology - March 3, 2024 Category: Food Science Authors: Lamia Cherrat Alexis Dijamentiuk Sara M El Kheir C écile Mangavel Annelore Elfassy Anne-Marie Revol-Junelles Fr édéric Borges Source Type: research
Microbiological safety of dry-cured fish from the raw material to the end of processing
In this study total aerobic colony count and Enterobacteriaceae, as proxy of process hygiene criteria, and detection of Listeria monocytogenes and concentration of histamine, as food safety criteria, were investigated in Salmo salar (salmon), Xiphias gladius (swordfish) and Thunnus albacares (yellowfin tuna), before, during, and at the end of a dry-curing process, performed in a dedicated cabinet, at controlled temperature, relative humidity and ventilation, up to 240 h. The microbiological parameters were investigated in the tested fish products by culture methods and shotgun metagenomic, while the presence of histamine, ...
Source: International Journal of Food Microbiology - March 3, 2024 Category: Food Science Authors: Valentina Indio Federica Savini Fausto Gardini Federica Barbieri Laura Prandini Yitagele Terefe Mekonnen Federico Tomasello Federica Giacometti Alessandro Seguino Andrea Serraino Alessandra De Cesare Source Type: research
A selection process based on the robustness of anti-Listeria monocytogenes activity reveals two strains of Carnobacterium maltaromaticum with biopreservation properties in cheese
In this study, the anti-Listeria monocytogenes EGDelux properties of a collection of 77 Carnobacterium maltaromaticum strains were investigated by high throughput competition assays under varying conditions of co-culture inoculation level, time interval between inoculation with C. maltaromaticum and L. monocytogenes, pH, and NaCl, resulting in 1309 different combinations of C. maltaromaticum strains and culture conditions. This screening led to the selection of two candidate strains with potent and robust anti-L. monocytogenes activities. Deferred growth inhibition assays followed by halo measurements, and liquid co-cultur...
Source: International Journal of Food Microbiology - March 3, 2024 Category: Food Science Authors: Lamia Cherrat Alexis Dijamentiuk Sara M El Kheir C écile Mangavel Annelore Elfassy Anne-Marie Revol-Junelles Fr édéric Borges Source Type: research
