A retrospective observational study of 1000 consecutive patients tested with the FilmArray ® Meningitis/Encephalitis panel: clinical diagnosis at discharge and microbiological findings
In this study, we investigated performance of FAME-p compared to comparator assays (CA), and for the first time, clinical diagnosis at discharge (CDD). 1000 consecutive patients with a cerebrospinal fluid (CSF) sample analyzed with FAME-p were identified. As CA, culture, polymerase chain reaction and cryptococcal antigen test were used. Medical records of patients were obtained. A CDD of CNS infection was made in 139 of 1000 CSF samples. FAME-p was positive in 66 samples with 44 viral and 22 bacterial agents. Thirteen FAME-p findings were not confirmed by CA, with four discrepant results remaining after comparison with the...
Source: Herpes - February 18, 2024 Category: Infectious Diseases Authors: Torgny Sunnerhagen Johan Wid én Sahar Handhal G ülşen Özkaya Şahin Source Type: research
Colonization of Raphanus sativus by human pathogenic microorganisms
In this study, we tested the susceptibility of radish (Raphanus sativus) to colonization by different HPMOs, including Escherichia coli PCM 2561, Salmonella enterica subsp. enterica PCM 2565, Listeria monocytogenes PCM 2191 and Bacillus cereus PCM 1948. We hypothesized that host plant roots containing bactericidal compounds are less prone to HPMO colonization than shoots and leaves. We also determined the effect of selected pathogens on radish growth to check host plant–microbe interactions. We found that one-week-old radish is susceptible to colonization by selected HPMOs, as the presence of the tested HPMOs was demonst...
Source: Frontiers in Microbiology - February 15, 2024 Category: Microbiology Source Type: research
Molecular Characteristics and Virulence Profile of Clinical Listeria monocytogenes Isolates in Northern Taiwan, 2009 –2019
Foodborne Pathogens and Disease, Ahead of Print. (Source: Foodborne Pathogens and Disease)
Source: Foodborne Pathogens and Disease - February 12, 2024 Category: Food Science Authors: Tsui-Ping Liu Lee-Chung Lin Shih-Cheng Chang Yu-Hsiang Ou Jang-Jih Lu Source Type: research
Evaluation of Enterococcus faecium NRRL B-2354 as a surrogate for Listeria monocytogenes during chlorine and peroxyacetic acid interventions in simulated apple dump tank water
This study validated the suitability of Enterococcus faecium NRRL B-2354 as a surrogate for Listeria monocytogenes during sanitizer interventions in dump tank water systems. E. faecium NRRL B-2354 inoculated on apples exhibited statistically equivalent susceptibility to L. monocytogenes when exposed to chlorine-based sanitizers (25-100 ppm free chlorine (FC)) and peroxyacetic acid (PAA, 20-80 ppm) in simulated dump tank water (SDTW) with 1000 ppm chemical oxygen demand (COD), resulting in 0.2-0.9 and 1.1-1.7 log CFU/apple reduction, respectively. Increasing the contact time did not affect sanitizer efficacies against E. fa...
Source: International Journal of Food Microbiology - February 11, 2024 Category: Food Science Authors: Yuan Su Xiaoye Shen Andrew Liu Mei-Jun Zhu Source Type: research
Evaluation of Enterococcus faecium NRRL B-2354 as a surrogate for Listeria monocytogenes during chlorine and peroxyacetic acid interventions in simulated apple dump tank water
This study validated the suitability of Enterococcus faecium NRRL B-2354 as a surrogate for Listeria monocytogenes during sanitizer interventions in dump tank water systems. E. faecium NRRL B-2354 inoculated on apples exhibited statistically equivalent susceptibility to L. monocytogenes when exposed to chlorine-based sanitizers (25-100 ppm free chlorine (FC)) and peroxyacetic acid (PAA, 20-80 ppm) in simulated dump tank water (SDTW) with 1000 ppm chemical oxygen demand (COD), resulting in 0.2-0.9 and 1.1-1.7 log CFU/apple reduction, respectively. Increasing the contact time did not affect sanitizer efficacies against E. fa...
Source: International Journal of Food Microbiology - February 11, 2024 Category: Food Science Authors: Yuan Su Xiaoye Shen Andrew Liu Mei-Jun Zhu Source Type: research
Evaluation of Enterococcus faecium NRRL B-2354 as a surrogate for Listeria monocytogenes during chlorine and peroxyacetic acid interventions in simulated apple dump tank water
This study validated the suitability of Enterococcus faecium NRRL B-2354 as a surrogate for Listeria monocytogenes during sanitizer interventions in dump tank water systems. E. faecium NRRL B-2354 inoculated on apples exhibited statistically equivalent susceptibility to L. monocytogenes when exposed to chlorine-based sanitizers (25-100 ppm free chlorine (FC)) and peroxyacetic acid (PAA, 20-80 ppm) in simulated dump tank water (SDTW) with 1000 ppm chemical oxygen demand (COD), resulting in 0.2-0.9 and 1.1-1.7 log CFU/apple reduction, respectively. Increasing the contact time did not affect sanitizer efficacies against E. fa...
Source: International Journal of Food Microbiology - February 11, 2024 Category: Food Science Authors: Yuan Su Xiaoye Shen Andrew Liu Mei-Jun Zhu Source Type: research
Corrigendum to "A stochastic approach for modelling the in-vitro effect of osmotic stress on growth dynamics and persistent cell formation in Listeria monocytogenes" [Int. J. Food Microbiol. 413 (2024) 110586]
Int J Food Microbiol. 2024 Feb 7:110614. doi: 10.1016/j.ijfoodmicro.2024.110614. Online ahead of print.NO ABSTRACTPMID:38331628 | DOI:10.1016/j.ijfoodmicro.2024.110614 (Source: International Journal of Food Microbiology)
Source: International Journal of Food Microbiology - February 8, 2024 Category: Food Science Authors: Luca Nalbone Salvatore Forgia Graziella Ziino Giorgia Sorrentino Filippo Giarratana Alessandro Giuffrida Source Type: research
Survival and growth behavior of common foodborne pathogens in falafel paste under different storage temperatures
Int J Food Microbiol. 2024 Mar 2;413:110609. doi: 10.1016/j.ijfoodmicro.2024.110609. Epub 2024 Feb 1.ABSTRACTFalafel is a popular breakfast food in the Middle East that has been recently involved in several outbreaks of foodborne illnesses. The aim of the study was to explore the growth behavior of Salmonella enterica, Escherichia coli O157:H7, Shigella sonnie, Shigella flexneri, Listeria monocytogenes and Staphylococcus aureus in falafel paste (FP) under different storage temperatures (4, 10, or 24 °C) for 14 days. FP (pH = 6.2, aw = 0.96) was inoculated with 5.0 to 6.0 log CFU/g of each of the pathogens separately. Salm...
Source: International Journal of Food Microbiology - February 8, 2024 Category: Food Science Authors: Murad A Al-Holy Amin N Olaimat Anas A Al-Nabulsi Hamzah Al-Qadiri Mahmoud H Abughoush Tareq M Osaili Mutamed Ayyash Maysa Alawneh Barbara A Rasco Source Type: research
Transfer of Salmonella, Escherichia coli O157:H7 and Listeria monocytogenes from contaminated soilless substrate and seeds to microgreens
This study suggests a high risk of pathogen population transferring to microgreens in case of seed or soilless substrate contamination when pathogen growth or survival is supported in plant nutrient solution.PMID:38325258 | DOI:10.1016/j.ijfoodmicro.2024.110612 (Source: International Journal of Food Microbiology)
Source: International Journal of Food Microbiology - February 7, 2024 Category: Food Science Authors: Sefa I şık B ülent Çetin Zeynal Topalcengiz Source Type: research
Degradation of < em > Listeria monocytogenes < /em > biofilm by phages belonging to the genus < em > Pecentumvirus < /em >
Appl Environ Microbiol. 2024 Feb 5:e0106223. doi: 10.1128/aem.01062-23. Online ahead of print.ABSTRACTListeria monocytogenes is a pathogenic foodborne bacterium that is a significant cause of mortality associated with foodborne illness and causes many food recalls attributed to a bacteriological cause. Their ability to form biofilms contributes to the persistence of Listeria spp. in food processing environments. When growing as biofilms, L. monocytogenes are more resistant to sanitizers used in the food industry, such as benzalkonium chloride (BAC), as well as to physical stresses like desiccation and starvation. Lytic pha...
Source: Applied and Environmental Microbiology - February 5, 2024 Category: Microbiology Authors: Stevan Cuci ć Tim Ells Anilda Guri Andrew M Kropinski Cezar M Khursigara Hany Anany Source Type: research
Impacts of water activity on survival of Listeria innocua and Enterococcus faecium NRRL B-2354 in almonds during steam treatments
Int J Food Microbiol. 2024 Mar 2;413:110592. doi: 10.1016/j.ijfoodmicro.2024.110592. Epub 2024 Jan 20.ABSTRACTRaw almonds have been associated with Salmonella outbreaks and multiple recalls related to Listeria monocytogenes contamination. While steam treatment has been approved for pasteurizing both conventional and organic whole almonds, there is limited understanding of how water activity (aw) influences the effectiveness of steam treatments in decontaminating almonds. Hence, this study aimed to assess and compare the efficacy of steam treatments against Listeria innocua and Enterococcus faecium NRRL B-2354, the known no...
Source: International Journal of Food Microbiology - February 3, 2024 Category: Food Science Authors: Zi Hua Bhim Bahadur Thapa Frank Younce Juming Tang Mei-Jun Zhu Source Type: research
Profiling and source tracking of the microbial populations and resistome present in fish products
This study investigated the microbial composition, prevalence of Listeria spp., and resistome structures in three catfish processing facilities in the southeastern United States. The 16S rRNA gene sequencing revealed that the observed richness and Shannon diversity index increased significantly from fish to fillet. Beta diversity analysis showed distinct clustering of microbial communities between fish, environment, and fillet samples. Fast expectation-maximization microbial source tracking (FEAST) algorithm demonstrated that the microbiota presents in the processing environment contributed 48.2 %, 62.4 %, and 53.7 % to th...
Source: International Journal of Food Microbiology - February 2, 2024 Category: Food Science Authors: Xiran Li Hongye Wang Hisham Abdelrahman Anita Kelly Luke Roy Luxin Wang Source Type: research
Efficacy of disinfectant and bacteriophage mixture against planktonic and biofilm state of Listeria monocytogenes to control in the food industry
Int J Food Microbiol. 2024 Mar 2;413:110587. doi: 10.1016/j.ijfoodmicro.2024.110587. Epub 2024 Jan 20.ABSTRACTFresh produce and animal-based products contaminated with Listeria monocytogenes have been the main cause of listeriosis outbreaks for many years. The present investigation explored the potential of combination treatment of disinfectants with a bacteriophage cocktail to control L. monocytogenes contamination in the food industry. A mixture of 1 minimal inhibitory concentration (MIC) of disinfectants (sodium hypochlorite [NaOCl], hydrogen peroxide [H2O2], and lactic acid [LA]) and multiplicity of infection (MOI) 100...
Source: International Journal of Food Microbiology - February 1, 2024 Category: Food Science Authors: Kye-Hwan Byun Sang Ha Han Min Woo Choi Byoung-Hu Kim Sang-Do Ha Source Type: research
Molecules, Vol. 29, Pages 685: Antibacterial and Antibiofilm Effects of Photodynamic Treatment with Curcuma L. and Trans-Cinnamaldehyde against Listeria monocytogenes
In conclusion, Cur demonstrated excellent photosensitizing properties against Lm in both planktonic and biofilm states. The efficacy of Ca was lower than that of Cur. However, Ca bears potent antibiofilm effects, which vary depending on the surface on which Lm resides. Therefore, this study may help identify more effective plant-based compounds to combat L. monocytogenes in an environmentally sustainable manner. (Source: Molecules)
Source: Molecules - February 1, 2024 Category: Chemistry Authors: Aleksandra Zimi ńska Izabela Lipska Joanna Gajewska Anna Draszanowska Manuel Sim ões Magdalena A. Olszewska Tags: Article Source Type: research
GSE220480 Transcriptome Sequencing of mice eosinophils upon Listeria monocytogenes (L.m.) infection
Contributor : Jun ZhouSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusMature eosinophils were differentiated from mouse bone marrow progenitorsWe performed transcriptome sequencing on Listeria monocytogenes infected eosinophils and resting eosinophils to shed light on the transcriptional changes of cytokines and chemokines. (Source: GEO: Gene Expression Omnibus)
Source: GEO: Gene Expression Omnibus - January 31, 2024 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Mus musculus Source Type: research
