Lassa Fever Research This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

The Perspectives of Healthcare Professionals on the Strategies, Challenges, and Community Responses to Health System Response and Interventions Towards Lassa Fever Infections and Mortality in Sierra Leone
(Source: Risk Management and Healthcare Policy)
Source: Risk Management and Healthcare Policy - May 7, 2024 Category: Health Management Tags: Risk Management and Healthcare Policy Source Type: research

Development of a non-infectious control for viral hemorrhagic fever PCR assays
by Matthew A. Knox, Collette Bromhead, David TS Hayman Assay validation is an essential component of disease surveillance testing, but can be problematic in settings where access to positive control material is limited and a safety risk for handlers. Here we describe a single non-infectious synthetic control that can help develop and validate the PCR based detection of the viral causes of Crimean-Congo hemorrhagic fever, Ebola virus disease, Lassa fever, Marburg virus disease and Rift Valley fever. We designed non-infectious synthetic DNA oligonucleotide sequences incorporating primer binding sites suitable for five assay...
Source: PLoS Neglected Tropical Diseases - April 22, 2024 Category: Tropical Medicine Authors: Matthew A. Knox Source Type: research

Viruses, Vol. 16, Pages 635: Pathogenic and Apathogenic Strains of Lymphocytic Choriomeningitis Virus Have Distinct Entry and Innate Immune Activation Pathways
. Lukashevich Lymphocytic choriomeningitis virus (LCMV) and Lassa virus (LASV) share many genetic and biological features including subtle differences between pathogenic and apathogenic strains. Despite remarkable genetic similarity, the viscerotropic WE strain of LCMV causes a fatal LASV fever-like hepatitis in non-human primates (NHPs) while the mouse-adapted Armstrong (ARM) strain of LCMV is deeply attenuated in NHPs and can vaccinate against LCMV-WE challenge. Here, we demonstrate that internalization of WE is more sensitive to the depletion of membrane cholesterol than ARM infection while ARM infection is more rel...
Source: Viruses - April 19, 2024 Category: Virology Authors: Dylan M. Johnson Nittaya Khakhum Min Wang Nikole L. Warner Jenny D. Jokinen Jason E. Comer Igor S. Lukashevich Tags: Article Source Type: research

YF17D-based vaccines - standing on the shoulders of a giant
Eur J Immunol. 2024 Apr 3:e2250133. doi: 10.1002/eji.202250133. Online ahead of print.ABSTRACTLive-attenuated yellow fever vaccine (YF17D) was developed in the 1930s as the first ever empirically derived human vaccine. Ninety years later, it is still a benchmark for vaccines made today. YF17D triggers a particularly broad and polyfunctional response engaging multiple arms of innate, humoral and cellular immunity. This unique immunogenicity translates into an extraordinary vaccine efficacy and outstanding longevity of protection, possibly by single-dose immunization. More recently, progress in molecular virology and synthet...
Source: European Journal of Immunology - April 4, 2024 Category: Allergy & Immunology Authors: Lorena Sanchez-Felipe Yeranddy A Alpizar Ji Ma Lotte Coelmont Kai Dallmeier Source Type: research

Spatio-temporal spread and evolution of Lassa virus in West Africa
Lassa fever is a hemorrhagic disease caused by Lassa virus (LASV), which has been classified by the World Health Organization as one of the top infectious diseases requiring prioritized research. Previous stud... (Source: BMC Infectious Diseases)
Source: BMC Infectious Diseases - March 14, 2024 Category: Infectious Diseases Authors: Xia Wang, Xianwei Ye, Ruihua Li, Xiaodong Zai, Mingda Hu, Shaoyan Wang, Hongguang Ren, Yuan Jin, Junjie Xu and Junjie Yue Tags: Research Source Type: research

MHC-I alleles mediate clearance and antibody response to the zoonotic Lassa virus in < i > Mastomys < /i > rodent reservoirs
by Ayodeji Olayemi, Dominik Werner Schmid, Ramona Fleischer, Kerstin Wilhelm, Alexander Christoph Heni, Nadine Mueller-Klein, Lavinia Haikukutu, Elisabeth Fichet-Calvet, Stephan G ünther, Simone Sommer West AfricanMastomys rodents are the primary reservoir of the zoonotic Lassa virus (LASV). The virus causes haemorrhagic Lassa fever and considerable mortality in humans. To date, the role ofMastomys immunogenetics in resistance to, and persistence of, LASV infections is largely unknown. Here, we investigated the role of Major Histocompatibility Complex class I (MHC-I) on LASV infection status (i.e., active vs. cleared inf...
Source: PLoS Neglected Tropical Diseases - February 29, 2024 Category: Tropical Medicine Authors: Ayodeji Olayemi Source Type: research

Lassa fever vaccine use cases and demand: Perspectives from select West African experts
Vaccine. 2024 Feb 17:S0264-410X(24)00203-2. doi: 10.1016/j.vaccine.2024.02.044. Online ahead of print.ABSTRACTLassa fever (LF) is a zoonotic viral hemorrhagic disease endemic to several West African countries. Approximately 300-500,000 cases occur annually across all ages with 10-20% case fatality rates. A LF vaccine is a recognized public health priority, with several candidates entering clinical trials. However, the perspectives of regional experts regarding critical vaccine properties, ideal delivery methods, and priority target populations remain unclear. Using a mixed methods approach with a standardized questionnaire...
Source: Vaccine - February 18, 2024 Category: Allergy & Immunology Authors: Lassan é Kaboré Clint Pecenka William P Hausdorff Source Type: research

An atlas of gross and histologic lesions and immunohistochemical immunoreactivity during the temporal progression of aerosolized Lassa virus induced hemorrhagic fever in cynomolgus macaques
Lassa virus (LASV) causes an acute multisystemic hemorrhagic fever in humans known as Lassa fever, which is endemic in several African countries. This manuscript focuses on the progression of disease in cynomolgus macaques challenged with aerosolized LASV and serially sampled for the development and progression of gross and histopathologic lesions. Gross lesions were first noted in tissues on day 6 and persisted throughout day 12. Viremia and histologic lesions were first noted on day 6 commencing with the pulmonary system and hemolymphatic system and progressing at later time points to include all systems. Immunoreactivit...
Source: Frontiers in cellular and infection microbiology - February 9, 2024 Category: Microbiology Source Type: research

Viruses, Vol. 16, Pages 266: The Importance of Lassa Fever and Its Disease Management in West Africa
The objective of this article is to revisit the importance and prevalence of LF in West Africa, with focus on Nigeria, and discuss current therapeutic approaches and ongoing vaccine development. In addition, we aim to emphasize the need for more scientific studies relating to LF-associated hearing loss, and to promote critical discussion about potential risks and benefits of vaccinating the population in endemic regions of West Africa. (Source: Viruses)
Source: Viruses - February 7, 2024 Category: Virology Authors: Rachel A. Reyna Kirsten E. Littlefield Nathan Shehu Tomoko Makishima Junki Maruyama Slobodan Paessler Tags: Review Source Type: research

Introduction of high containment laboratories in abroad
Uirusu. 2022;72(2):139-148. doi: 10.2222/jsv.72.139.ABSTRACTRecently, outbreaks of highly pathogenic viruses, such as those of Ebola and Lassa viruses, have become a global public health issue. Such viruses must be handled in biosafety level 4 (BSL-4) laboratories. Currently, 62 BSL-4 laboratories are in operation, under construction, or planned in 24 counties. In this review, I provide an overview of the current status and characteristics of BSL-4 facilities in abroad and introduce my research on the wild-type Ebola virus at the BSL-4 facility in the USA.PMID:38220158 | DOI:10.2222/jsv.72.139 (Source: Uirusu. Journal of Virology)
Source: Uirusu. Journal of Virology - January 14, 2024 Category: Virology Authors: Wakako Furuyama Asuka Nanbo Source Type: research