Atom counting based on Voronoi averaged STEM intensities using a crosstalk correction scheme
Ultramicroscopy. 2023 Oct 17;256:113867. doi: 10.1016/j.ultramic.2023.113867. Online ahead of print.ABSTRACTIf quantitative scanning transmission electron microscopy is used for very precise thickness measurements with atomic resolution, it is commonly referred to as »atom counting«. Due to scattering and the finite probe extent the signal recorded in one atomic column is dependent not only on its own height but also on the height of its neighbours. Especially for thicker specimens this crosstalk effect can have significant impact on the measured intensity. If it is not appropriately accounted for in the evaluation, it c...
Source: Ultramicroscopy - October 23, 2023 Category: Laboratory Medicine Authors: Florian F Krause Andreas Rosenauer Source Type: research
Atom counting based on Voronoi averaged STEM intensities using a crosstalk correction scheme
Ultramicroscopy. 2023 Oct 17;256:113867. doi: 10.1016/j.ultramic.2023.113867. Online ahead of print.ABSTRACTIf quantitative scanning transmission electron microscopy is used for very precise thickness measurements with atomic resolution, it is commonly referred to as »atom counting«. Due to scattering and the finite probe extent the signal recorded in one atomic column is dependent not only on its own height but also on the height of its neighbours. Especially for thicker specimens this crosstalk effect can have significant impact on the measured intensity. If it is not appropriately accounted for in the evaluation, it c...
Source: Ultramicroscopy - October 23, 2023 Category: Laboratory Medicine Authors: Florian F Krause Andreas Rosenauer Source Type: research
Atom counting based on Voronoi averaged STEM intensities using a crosstalk correction scheme
Ultramicroscopy. 2023 Oct 17;256:113867. doi: 10.1016/j.ultramic.2023.113867. Online ahead of print.ABSTRACTIf quantitative scanning transmission electron microscopy is used for very precise thickness measurements with atomic resolution, it is commonly referred to as »atom counting«. Due to scattering and the finite probe extent the signal recorded in one atomic column is dependent not only on its own height but also on the height of its neighbours. Especially for thicker specimens this crosstalk effect can have significant impact on the measured intensity. If it is not appropriately accounted for in the evaluation, it c...
Source: Ultramicroscopy - October 23, 2023 Category: Laboratory Medicine Authors: Florian F Krause Andreas Rosenauer Source Type: research
Atom counting based on Voronoi averaged STEM intensities using a crosstalk correction scheme
Ultramicroscopy. 2023 Oct 17;256:113867. doi: 10.1016/j.ultramic.2023.113867. Online ahead of print.ABSTRACTIf quantitative scanning transmission electron microscopy is used for very precise thickness measurements with atomic resolution, it is commonly referred to as »atom counting«. Due to scattering and the finite probe extent the signal recorded in one atomic column is dependent not only on its own height but also on the height of its neighbours. Especially for thicker specimens this crosstalk effect can have significant impact on the measured intensity. If it is not appropriately accounted for in the evaluation, it c...
Source: Ultramicroscopy - October 23, 2023 Category: Laboratory Medicine Authors: Florian F Krause Andreas Rosenauer Source Type: research
Optimizing experimental parameters for orbital mapping
Ultramicroscopy. 2023 Oct 18;256:113866. doi: 10.1016/j.ultramic.2023.113866. Online ahead of print.ABSTRACTA new material characterization technique is emerging for the transmission electron microscope (TEM). Using electron energy-loss spectroscopy, real space mappings of the underlying electronic transitions in the sample, so called orbital maps, can be produced. Thus, unprecedented insight into the electronic orbitals responsible for most of the electrical, magnetic and optical properties of bulk materials can be gained. However, the incredibly demanding requirements on spatial as well as spectral resolution paired with...
Source: Ultramicroscopy - October 22, 2023 Category: Laboratory Medicine Authors: Manuel Ederer Stefan L öffler Source Type: research
The effect of nanochannel length on in situ loading times of diffusion-propelled nanoparticles in liquid cell electron microscopy
Ultramicroscopy. 2023 Oct 5;255:113865. doi: 10.1016/j.ultramic.2023.113865. Online ahead of print.ABSTRACTLiquid cell transmission electron microscopy is a powerful tool for visualizing nanoparticle (NP) assemblies in liquid environments with nanometer resolution. However, it remains a challenge to control the NP concentration in the high aspect ratio liquid enclosure where the diffusion of dispersed NPs is affected by the exposed surface of the liquid cell walls. Here, we introduce a semi-empirical model based on the 1D diffusion equation, to predict the NP loading time as they pass through the nanochannel into the imagi...
Source: Ultramicroscopy - October 19, 2023 Category: Laboratory Medicine Authors: Peter Kunnas Niels de Jonge Joseph P Patterson Source Type: research
The effect of nanochannel length on in situ loading times of diffusion-propelled nanoparticles in liquid cell electron microscopy
Ultramicroscopy. 2023 Oct 5;255:113865. doi: 10.1016/j.ultramic.2023.113865. Online ahead of print.ABSTRACTLiquid cell transmission electron microscopy is a powerful tool for visualizing nanoparticle (NP) assemblies in liquid environments with nanometer resolution. However, it remains a challenge to control the NP concentration in the high aspect ratio liquid enclosure where the diffusion of dispersed NPs is affected by the exposed surface of the liquid cell walls. Here, we introduce a semi-empirical model based on the 1D diffusion equation, to predict the NP loading time as they pass through the nanochannel into the imagi...
Source: Ultramicroscopy - October 19, 2023 Category: Laboratory Medicine Authors: Peter Kunnas Niels de Jonge Joseph P Patterson Source Type: research
The effect of nanochannel length on in situ loading times of diffusion-propelled nanoparticles in liquid cell electron microscopy
Ultramicroscopy. 2023 Oct 5;255:113865. doi: 10.1016/j.ultramic.2023.113865. Online ahead of print.ABSTRACTLiquid cell transmission electron microscopy is a powerful tool for visualizing nanoparticle (NP) assemblies in liquid environments with nanometer resolution. However, it remains a challenge to control the NP concentration in the high aspect ratio liquid enclosure where the diffusion of dispersed NPs is affected by the exposed surface of the liquid cell walls. Here, we introduce a semi-empirical model based on the 1D diffusion equation, to predict the NP loading time as they pass through the nanochannel into the imagi...
Source: Ultramicroscopy - October 19, 2023 Category: Laboratory Medicine Authors: Peter Kunnas Niels de Jonge Joseph P Patterson Source Type: research
Scanning precession electron diffraction data analysis approaches for phase mapping of precipitates in aluminium alloys
Ultramicroscopy. 2023 Oct 6;255:113861. doi: 10.1016/j.ultramic.2023.113861. Online ahead of print.ABSTRACTMapping the spatial distribution of crystal phases with nm-scale spatial resolution is an important characterisation task in studies of multi-phase materials. One popular approach is to use scanning precession electron diffraction which enables semi-automatic phase mapping at the nanoscale by collecting a single precession electron diffraction pattern at every probe position over regions spanning up to a few micrometers. For a successful phase mapping each diffraction pattern must be correctly identified. In this work...
Source: Ultramicroscopy - October 18, 2023 Category: Laboratory Medicine Authors: E Thronsen T Bergh T I Thorsen E F Christiansen J Frafjord P Crout A T J van Helvoort P A Midgley R Holmestad Source Type: research
Scanning precession electron diffraction data analysis approaches for phase mapping of precipitates in aluminium alloys
Ultramicroscopy. 2023 Oct 6;255:113861. doi: 10.1016/j.ultramic.2023.113861. Online ahead of print.ABSTRACTMapping the spatial distribution of crystal phases with nm-scale spatial resolution is an important characterisation task in studies of multi-phase materials. One popular approach is to use scanning precession electron diffraction which enables semi-automatic phase mapping at the nanoscale by collecting a single precession electron diffraction pattern at every probe position over regions spanning up to a few micrometers. For a successful phase mapping each diffraction pattern must be correctly identified. In this work...
Source: Ultramicroscopy - October 18, 2023 Category: Laboratory Medicine Authors: E Thronsen T Bergh T I Thorsen E F Christiansen J Frafjord P Crout A T J van Helvoort P A Midgley R Holmestad Source Type: research
Self-supervised noise modeling and sparsity guided electron tomography volumetric image denoising
Ultramicroscopy. 2023 Oct 5;255:113860. doi: 10.1016/j.ultramic.2023.113860. Online ahead of print.ABSTRACTCryo-Electron Tomography (cryo-ET) is a revolutionary technique for visualizing macromolecular structures in near-native states. However, the physical limitations of imaging instruments lead to cryo-ET volumetric images with very low Signal-to-Noise Ratio (SNR) with complex noise, which has a side effect on the downstream analysis of the characteristics of observed macromolecules. Additionally, existing methods for image denoising are difficult to be well generalized to the complex noise in cryo-ET volumes. In this wo...
Source: Ultramicroscopy - October 16, 2023 Category: Laboratory Medicine Authors: Zhidong Yang Dawei Zang Hongjia Li Zhao Zhang Fa Zhang Renmin Han Source Type: research
Self-supervised noise modeling and sparsity guided electron tomography volumetric image denoising
Ultramicroscopy. 2023 Oct 5;255:113860. doi: 10.1016/j.ultramic.2023.113860. Online ahead of print.ABSTRACTCryo-Electron Tomography (cryo-ET) is a revolutionary technique for visualizing macromolecular structures in near-native states. However, the physical limitations of imaging instruments lead to cryo-ET volumetric images with very low Signal-to-Noise Ratio (SNR) with complex noise, which has a side effect on the downstream analysis of the characteristics of observed macromolecules. Additionally, existing methods for image denoising are difficult to be well generalized to the complex noise in cryo-ET volumes. In this wo...
Source: Ultramicroscopy - October 16, 2023 Category: Laboratory Medicine Authors: Zhidong Yang Dawei Zang Hongjia Li Zhao Zhang Fa Zhang Renmin Han Source Type: research
Real-time electron clustering in an event-driven hybrid pixel detector
Ultramicroscopy. 2023 Oct 5;255:113864. doi: 10.1016/j.ultramic.2023.113864. Online ahead of print.ABSTRACTEvent-driven hybrid pixel detectors with nanosecond time resolution have opened up novel pathways in modern ultrafast electron microscopy, for example in hyperspectral electron-energy loss spectroscopy or free-electron quantum optics. However, the impinging electrons typically excite more than one pixel of the device, and an efficient algorithm is therefore needed to convert the measured pixel hits to real single-electron events. Here we present a robust clustering algorithm that is fast enough to find clusters in a c...
Source: Ultramicroscopy - October 15, 2023 Category: Laboratory Medicine Authors: J Kuttruff J Holder Y Meng P Baum Source Type: research
Real-time electron clustering in an event-driven hybrid pixel detector
Ultramicroscopy. 2023 Oct 5;255:113864. doi: 10.1016/j.ultramic.2023.113864. Online ahead of print.ABSTRACTEvent-driven hybrid pixel detectors with nanosecond time resolution have opened up novel pathways in modern ultrafast electron microscopy, for example in hyperspectral electron-energy loss spectroscopy or free-electron quantum optics. However, the impinging electrons typically excite more than one pixel of the device, and an efficient algorithm is therefore needed to convert the measured pixel hits to real single-electron events. Here we present a robust clustering algorithm that is fast enough to find clusters in a c...
Source: Ultramicroscopy - October 15, 2023 Category: Laboratory Medicine Authors: J Kuttruff J Holder Y Meng P Baum Source Type: research
Modeling scanning near-field optical photons scattered from an atomic force microscope for quantum metrology
Ultramicroscopy. 2023 Oct 6;255:113863. doi: 10.1016/j.ultramic.2023.113863. Online ahead of print.ABSTRACTScattering scanning near-field optical microscopy (s-SNOM) is a promising technique for overcoming Abbe diffraction limit and substantially enhancing the spatial resolution in spectroscopic imaging. The s-SNOM works by exposing an atomic force microscope (AFM) tip to an optical electromagnetic (EM) field, while the tip is so close to a dielectric sample that the incident beam lies within the near-field regime and displays nonlinear behavior. We suggest replacing the incident EM field by photons generated by a single p...
Source: Ultramicroscopy - October 14, 2023 Category: Laboratory Medicine Authors: Soheil Khajavi Zahra Shaterzadeh-Yazdi Ali Eghrari Mohammad Neshat Source Type: research
