Time calibration studies for the Timepix3 hybrid pixel detector in electron microscopy
Ultramicroscopy. 2023 Dec 2;257:113889. doi: 10.1016/j.ultramic.2023.113889. Online ahead of print.ABSTRACTDirect electron detection is currently revolutionizing many fields of electron microscopy due to its lower noise, its reduced point-spread function, and its increased quantum efficiency. More specifically to this work, Timepix3 is a hybrid-pixel direct electron detector capable of outputting temporal information of individual hits in its pixel array. Its architecture results in a data-driven detector, also called event-based, in which individual hits trigger the data off the chip for readout as fast as possible. The p...
Source: Ultramicroscopy - December 6, 2023 Category: Laboratory Medicine Authors: Yves Auad Jassem Baaboura Jean-Denis Blazit Marcel Tenc é Odile St éphan Mathieu Kociak Luiz H G Tizei Source Type: research
Correlating electrochemical stimulus to structural change in liquid electron microscopy videos using the structural dissimilarity metric
In this study, DSSIM analysis is applied to two in-situ LCTEM videos to demonstrate how to implement this algorithm and interpret the results. We show DSSIM analysis can be used as a visualization tool for qualitative data analysis by highlighting structural changes which are easily missed when viewing the raw data. Furthermore, we demonstrate how DSSIM analysis can serve as a quantitative metric and efficiently convert 3-dimensional microscopy videos to 1-dimenional plots which makes it easy to interpret and compare events occurring at different timepoints in a video. In the analyses presented here, DSSIM is used to direc...
Source: Ultramicroscopy - December 6, 2023 Category: Laboratory Medicine Authors: Justin T Mulvey Katen P Iyer Tom às Ortega Jovany G Merham Yevheniy Pivak Hongyu Sun Allon I Hochbaum Joseph P Patterson Source Type: research
Weighted Burgers Vector analysis of orientation fields from high-angular resolution electron backscatter diffraction
Ultramicroscopy. 2023 Nov 24;257:113893. doi: 10.1016/j.ultramic.2023.113893. Online ahead of print.ABSTRACTThe Weighted Burgers Vector (WBV) method can extract information about dislocation types and densities present in distorted crystalline materials from electron backscatter diffraction (EBSD) maps, using no assumptions about which slip systems might be present. Furthermore, high-angular resolution EBSD (HR-EBSD) uses a cross-correlation procedure to increase the angular precision of EBSD measurements by an order of magnitude compared to conventional EBSD. However, the WBV technique has not previously been applied to H...
Source: Ultramicroscopy - December 6, 2023 Category: Laboratory Medicine Authors: Joe Gardner David Wallis Lars N Hansen John Wheeler Source Type: research
Time calibration studies for the Timepix3 hybrid pixel detector in electron microscopy
Ultramicroscopy. 2023 Dec 2;257:113889. doi: 10.1016/j.ultramic.2023.113889. Online ahead of print.ABSTRACTDirect electron detection is currently revolutionizing many fields of electron microscopy due to its lower noise, its reduced point-spread function, and its increased quantum efficiency. More specifically to this work, Timepix3 is a hybrid-pixel direct electron detector capable of outputting temporal information of individual hits in its pixel array. Its architecture results in a data-driven detector, also called event-based, in which individual hits trigger the data off the chip for readout as fast as possible. The p...
Source: Ultramicroscopy - December 6, 2023 Category: Laboratory Medicine Authors: Yves Auad Jassem Baaboura Jean-Denis Blazit Marcel Tenc é Odile St éphan Mathieu Kociak Luiz H G Tizei Source Type: research
Noise-dependent bias in quantitative STEM-EMCD experiments revealed by bootstrapping
Ultramicroscopy. 2023 Nov 24;257:113891. doi: 10.1016/j.ultramic.2023.113891. Online ahead of print.ABSTRACTElectron magnetic circular dichroism (EMCD) is a powerful technique for estimating element-specific magnetic moments of materials on nanoscale with the potential to reach atomic resolution in transmission electron microscopes. However, the fundamentally weak EMCD signal strength complicates quantification of magnetic moments, as this requires very high precision, especially in the denominator of the sum rules. Here, we employ a statistical resampling technique known as bootstrapping to an experimental EMCD dataset to...
Source: Ultramicroscopy - December 3, 2023 Category: Laboratory Medicine Authors: Hasan Ali Jan Rusz Daniel E B ürgler Roman Adam Claus M Schneider Cheuk-Wai Tai Thomas Thersleff Source Type: research
Noise-dependent bias in quantitative STEM-EMCD experiments revealed by bootstrapping
Ultramicroscopy. 2023 Nov 24;257:113891. doi: 10.1016/j.ultramic.2023.113891. Online ahead of print.ABSTRACTElectron magnetic circular dichroism (EMCD) is a powerful technique for estimating element-specific magnetic moments of materials on nanoscale with the potential to reach atomic resolution in transmission electron microscopes. However, the fundamentally weak EMCD signal strength complicates quantification of magnetic moments, as this requires very high precision, especially in the denominator of the sum rules. Here, we employ a statistical resampling technique known as bootstrapping to an experimental EMCD dataset to...
Source: Ultramicroscopy - December 3, 2023 Category: Laboratory Medicine Authors: Hasan Ali Jan Rusz Daniel E B ürgler Roman Adam Claus M Schneider Cheuk-Wai Tai Thomas Thersleff Source Type: research
Noise-dependent bias in quantitative STEM-EMCD experiments revealed by bootstrapping
Ultramicroscopy. 2023 Nov 24;257:113891. doi: 10.1016/j.ultramic.2023.113891. Online ahead of print.ABSTRACTElectron magnetic circular dichroism (EMCD) is a powerful technique for estimating element-specific magnetic moments of materials on nanoscale with the potential to reach atomic resolution in transmission electron microscopes. However, the fundamentally weak EMCD signal strength complicates quantification of magnetic moments, as this requires very high precision, especially in the denominator of the sum rules. Here, we employ a statistical resampling technique known as bootstrapping to an experimental EMCD dataset to...
Source: Ultramicroscopy - December 3, 2023 Category: Laboratory Medicine Authors: Hasan Ali Jan Rusz Daniel E B ürgler Roman Adam Claus M Schneider Cheuk-Wai Tai Thomas Thersleff Source Type: research
Accurate magnification determination for cryoEM using gold
Ultramicroscopy. 2023 Nov 15;256:113883. doi: 10.1016/j.ultramic.2023.113883. Online ahead of print.ABSTRACTDetermining the correct magnified pixel size of single-particle cryoEM micrographs is necessary to maximize resolution and enable accurate model building. Here we describe a simple and rapid procedure for determining the absolute magnification in an electron cryomicroscope to a precision of <0.5%. We show how to use the atomic lattice spacings of crystals of thin and readily available test specimens, such as gold, as an absolute reference to determine magnification for both room temperature and cryogenic imaging. ...
Source: Ultramicroscopy - November 26, 2023 Category: Laboratory Medicine Authors: Joshua L Dickerson Erin Leahy Mathew J Peet Katerina Naydenova Christopher J Russo Source Type: research
Accurate magnification determination for cryoEM using gold
Ultramicroscopy. 2023 Nov 15;256:113883. doi: 10.1016/j.ultramic.2023.113883. Online ahead of print.ABSTRACTDetermining the correct magnified pixel size of single-particle cryoEM micrographs is necessary to maximize resolution and enable accurate model building. Here we describe a simple and rapid procedure for determining the absolute magnification in an electron cryomicroscope to a precision of <0.5%. We show how to use the atomic lattice spacings of crystals of thin and readily available test specimens, such as gold, as an absolute reference to determine magnification for both room temperature and cryogenic imaging. ...
Source: Ultramicroscopy - November 26, 2023 Category: Laboratory Medicine Authors: Joshua L Dickerson Erin Leahy Mathew J Peet Katerina Naydenova Christopher J Russo Source Type: research
Accurate magnification determination for cryoEM using gold
Ultramicroscopy. 2023 Nov 15;256:113883. doi: 10.1016/j.ultramic.2023.113883. Online ahead of print.ABSTRACTDetermining the correct magnified pixel size of single-particle cryoEM micrographs is necessary to maximize resolution and enable accurate model building. Here we describe a simple and rapid procedure for determining the absolute magnification in an electron cryomicroscope to a precision of <0.5%. We show how to use the atomic lattice spacings of crystals of thin and readily available test specimens, such as gold, as an absolute reference to determine magnification for both room temperature and cryogenic imaging. ...
Source: Ultramicroscopy - November 26, 2023 Category: Laboratory Medicine Authors: Joshua L Dickerson Erin Leahy Mathew J Peet Katerina Naydenova Christopher J Russo Source Type: research
Accurate magnification determination for cryoEM using gold
Ultramicroscopy. 2023 Nov 15;256:113883. doi: 10.1016/j.ultramic.2023.113883. Online ahead of print.ABSTRACTDetermining the correct magnified pixel size of single-particle cryoEM micrographs is necessary to maximize resolution and enable accurate model building. Here we describe a simple and rapid procedure for determining the absolute magnification in an electron cryomicroscope to a precision of <0.5%. We show how to use the atomic lattice spacings of crystals of thin and readily available test specimens, such as gold, as an absolute reference to determine magnification for both room temperature and cryogenic imaging. ...
Source: Ultramicroscopy - November 26, 2023 Category: Laboratory Medicine Authors: Joshua L Dickerson Erin Leahy Mathew J Peet Katerina Naydenova Christopher J Russo Source Type: research
Accurate magnification determination for cryoEM using gold
Ultramicroscopy. 2023 Nov 15;256:113883. doi: 10.1016/j.ultramic.2023.113883. Online ahead of print.ABSTRACTDetermining the correct magnified pixel size of single-particle cryoEM micrographs is necessary to maximize resolution and enable accurate model building. Here we describe a simple and rapid procedure for determining the absolute magnification in an electron cryomicroscope to a precision of <0.5%. We show how to use the atomic lattice spacings of crystals of thin and readily available test specimens, such as gold, as an absolute reference to determine magnification for both room temperature and cryogenic imaging. ...
Source: Ultramicroscopy - November 26, 2023 Category: Laboratory Medicine Authors: Joshua L Dickerson Erin Leahy Mathew J Peet Katerina Naydenova Christopher J Russo Source Type: research
Accurate magnification determination for cryoEM using gold
Ultramicroscopy. 2023 Nov 15;256:113883. doi: 10.1016/j.ultramic.2023.113883. Online ahead of print.ABSTRACTDetermining the correct magnified pixel size of single-particle cryoEM micrographs is necessary to maximize resolution and enable accurate model building. Here we describe a simple and rapid procedure for determining the absolute magnification in an electron cryomicroscope to a precision of <0.5%. We show how to use the atomic lattice spacings of crystals of thin and readily available test specimens, such as gold, as an absolute reference to determine magnification for both room temperature and cryogenic imaging. ...
Source: Ultramicroscopy - November 26, 2023 Category: Laboratory Medicine Authors: Joshua L Dickerson Erin Leahy Mathew J Peet Katerina Naydenova Christopher J Russo Source Type: research
Interfacial excess of solutes across phase boundaries using atom probe microscopy
Ultramicroscopy. 2023 Nov 11;256:113885. doi: 10.1016/j.ultramic.2023.113885. Online ahead of print.ABSTRACTThree-dimensional elemental mapping in atom probe microscopy provides invaluable insights into the structure and composition of interfaces in materials. Quasi-atomic resolution facilitates access to the solute decoration of grain boundaries, advancing the knowledge on local segregation and depletion phenomena. More recent developments unlocked three-dimensional mapping of the interfacial excess across grain boundaries. Such detailed understanding of the local structure and composition of these interfaces enabled adva...
Source: Ultramicroscopy - November 25, 2023 Category: Laboratory Medicine Authors: F Theska S Primig Source Type: research
Momentum transfer resolved electron correlation microscopy
Ultramicroscopy. 2023 Nov 11;256:113886. doi: 10.1016/j.ultramic.2023.113886. Online ahead of print.ABSTRACTElectron correlation microscopy (ECM) characterizes local structural relaxation dynamics in fluctuating systems like supercooled liquids with nanometer spatial resolution. We have developed a new type of ECM technique that provides moderate resolution in momentum transfer or k space using five-dimensional scanning transmission electron microscopy. k-resolved ECM on a Pt57.5Cu14.7Ni5.3P22.5 metallic supercooled liquids measures rich spatial and momentum structure in the relaxation time data τ(r,k). Relaxation time ma...
Source: Ultramicroscopy - November 24, 2023 Category: Laboratory Medicine Authors: Shuoyuan Huang Paul M Voyles Source Type: research
