Construction of a Recombinant Phage-vaccine Capable of Reducing the Growth Rate of an Established LL2 Tumor Model.
In conclusion, EM-L2 displaying phage particles could be deemed as an encouraging strategy in contemporary cancer immunotherapy. PMID: 29908541 [PubMed - in process] (Source: Iranian Journal of Allergy, Asthma and Immunology)
Source: Iranian Journal of Allergy, Asthma and Immunology - June 1, 2018 Category: Allergy & Immunology Authors: Asadi-Ghalehni M, Rasaee MJ, Namvar Asl N, Khosravani M, Rajabibazl M, Khalili S, Modjtahedi H, Sadroddiny E Tags: Iran J Allergy Asthma Immunol Source Type: research

miR-1224 Expression Is Increased in Human Macrophages after Infection with Bacillus Calmette-Gu érin (BCG).
This study highlighted the potential roles of miRNAs in host responses upon mycobacterium infection. PMID: 29908542 [PubMed - in process] (Source: Iranian Journal of Allergy, Asthma and Immunology)
Source: Iranian Journal of Allergy, Asthma and Immunology - June 1, 2018 Category: Allergy & Immunology Authors: Alipoor SD, Mortaz E, Tabarsi P, Marjani M, Varahram M, Folkerts G, Garssen J, Adcock IM Tags: Iran J Allergy Asthma Immunol Source Type: research

T Helper 22 Pathway Evaluation in Type 1 Diabetes and Its Complications.
Abstract A subset of CD4+ T cells named T helper (Th)22 cells play some pathogenic roles in some autoimmune disorders such as type 1 diabetes (T1D). We aimed to study the correlation between the circulatory number of these cells and serum levels of its related cytokines with T1D as well as diabetic complications including metabolic control, atherosclerosis, and nephropathy. Fortynine patients with T1D and 43 healthy controls underwent the assessment of circulatory number of Th22 cells (by flow cytometry), serum level of Th22 related cytokines including Interleukin-22 (IL-22), Interleukin-10 (IL-10), Transforming g...
Source: Iranian Journal of Allergy, Asthma and Immunology - June 1, 2018 Category: Allergy & Immunology Authors: Akbari H, Ghardashi M, Soleimani A, Mohammadi H, Nikoueinejad H Tags: Iran J Allergy Asthma Immunol Source Type: research

Scrutinizing the Expression and Blockade of Inhibitory Molecules Expressed on T Cells from Acute Myeloid Leukemia Patients.
In conclusion, our data indicate that CD4+ and CD8+ T cells from AML patients appeared to be exhausted and blockade of some immune checkpoints can improve the proliferation of those cells. PMID: 29908544 [PubMed - in process] (Source: Iranian Journal of Allergy, Asthma and Immunology)
Source: Iranian Journal of Allergy, Asthma and Immunology - June 1, 2018 Category: Allergy & Immunology Authors: Abdolmaleki M, Mojtabavi N, Zavvar M, Vaezi M, Noorbakhsh F, Nicknam MH Tags: Iran J Allergy Asthma Immunol Source Type: research

Expression of TNF- and HNRNPL-related Immunoregulatory Long Non-coding RNA (THRIL) in Acute Myeloid Leukemia: Is There Any Correlation?
In conclusion, our study showed that THRIL cannot be used as an informative biomarker for AML diagnosis, however, our results need to be clarify by evolution of more cases. PMID: 29908545 [PubMed - in process] (Source: Iranian Journal of Allergy, Asthma and Immunology)
Source: Iranian Journal of Allergy, Asthma and Immunology - June 1, 2018 Category: Allergy & Immunology Authors: Sayad A, Hajifathali A, Omrani MD, Arsang-Jang S, Hamidieh AA, Taheri M Tags: Iran J Allergy Asthma Immunol Source Type: research

Sublingual Immunotherapy with Sal k1 Expressing Lactococcus lactis Down-regulates Th2 Immune Responses in Balb/c Mice.
This study revealed that Th2 immune responses are reduced in allergic mice after feeding them with allergen expressing probiotic bacteria as a SLIT approach. Since the safety of this procedure was previously approved, thus, it seems that a similar protocol using human based probiotics could be applied for Salsola kali sensitive patients. PMID: 29908546 [PubMed - in process] (Source: Iranian Journal of Allergy, Asthma and Immunology)
Source: Iranian Journal of Allergy, Asthma and Immunology - June 1, 2018 Category: Allergy & Immunology Authors: Ghasemi Z, Varasteh AR, Moghadam M, Jalali SA, Anissian A, Sankian M Tags: Iran J Allergy Asthma Immunol Source Type: research

Oral Immunotherapy to Wheat in Allergic Asthmatic Female: A Case Report.
We present a case of a 32-yearold female patient, who worked for 5 years in wheat and bakery products industry, who developed an occupational asthma and chronic urticaria after flour inhalation or ingestion of foods that containit. The patient underwent wheat  OIT, that  was well-tolerated with no severe reaction during treatment. We may conclude that wheat OIT is a safe therapy and may induce symptoms improvement in allergic asthma and urticaria in patients with wheat allergy. Wheat OIT may induce tolerance to allergic patients. PMID: 29908547 [PubMed - in process] (Source: Iranian Journal of Allergy, Asthma and Immunology)
Source: Iranian Journal of Allergy, Asthma and Immunology - June 1, 2018 Category: Allergy & Immunology Authors: Muntean IA, Bocsan IC, Deleanu D Tags: Iran J Allergy Asthma Immunol Source Type: research

FEF25-75 and Asthma in Clinical Practice.
Abstract No Abstract. PMID: 29908548 [PubMed - in process] (Source: Iranian Journal of Allergy, Asthma and Immunology)
Source: Iranian Journal of Allergy, Asthma and Immunology - June 1, 2018 Category: Allergy & Immunology Authors: Ciprandi G, Gallo F, Cirillo I Tags: Iran J Allergy Asthma Immunol Source Type: research

What Immunological Defects Predispose to Non-tuberculosis Mycobacterial Infections?
Abstract Nontuberculous mycobacteria (NTM) are categorized as one of the large and diverse groups of environmental organisms which are abundant in water and soil.  NTM cause a variety of diseases in humans that mainly affect the lung. A predisposition to pulmonary NTM is evident in patients with parenchymal structural diseases including bronchiectasis, emphysema, tuberculosis (TB), cystic fibrosis (CF), rheumatologic lung diseases and other chronic diseases with pulmonary manifestations. Lung infections are not the only consequences of being infected by NTM as they can also infect skin and soft tissue and may...
Source: Iranian Journal of Allergy, Asthma and Immunology - April 1, 2018 Category: Allergy & Immunology Authors: Mortaz E, Moloudizargari M, Varahram M, Movassaghi M, Garssen J, Kazempour Dizagie M, Mirsaeidi M, Adcock IM Tags: Iran J Allergy Asthma Immunol Source Type: research

Effects of Nerve Growth Factor shRNA Inhibition on Asthma Phenotypes in a Mouse Model of Asthma.
In this study, we aimed at investigating the effect of NGF inhibition on AHR and other asthma phenotypes in a mouse model of asthma. 12 mice in each group were injected with lentiviral vectors expressing non-targeting shRNA (sham shRNA), targeting NGF (shRNA-1 and shRNA-2), or normal saline for control before the asthma models were established. Peak inspiratory pressure (PIP), NGF levels in bronchoalveolar lavage fluid (BALF), and bronchoconstriction in response to acetylcholine (ACh) were measured. Immunohistochemistry semi-quantitative analysis of muscarinic acetylcholine receptor M3 (mAChR M3) and alpha-smooth muscle ac...
Source: Iranian Journal of Allergy, Asthma and Immunology - April 1, 2018 Category: Allergy & Immunology Authors: Wu J, Chen B, Li W, Xiao Y Tags: Iran J Allergy Asthma Immunol Source Type: research

IgE Sensitization to Inhalant Allergens and Its Association with Allergic Diseases in Adults.
The objective of this study was to assess IgE-mediated sensitization to inhalant allergens in allergic and non-allergic adults as well as the evaluation of its association with allergic diseases. This cross-sectional study was conducted from 2013 to 2016 in 604 allergic and 102 non-allergic adults selected from blood donor volunteers in Tehran, Iran. After taking informed consent, a standard questionnaire was filled to determine asthma, allergic rhinitis and/or conjunctivitis and atopic dermatitis in participants. Specific IgE assay to common inhalant allergens was performed for all subjects. Logistic regression analysis w...
Source: Iranian Journal of Allergy, Asthma and Immunology - April 1, 2018 Category: Allergy & Immunology Authors: Shokouhi Shoormasti R, Fazlollahi MR, Kazemnejad A, Tayebi B, Nadali F, Sharif Shoushtari M, Khandan Alamdari S, Moslemi M, Movehdi M, Mari A, Pourpak Z, Moin M Tags: Iran J Allergy Asthma Immunol Source Type: research

Production of Recombinant Protein of Salsola Kali (Sal k1) Pollen Allergen in Lactococcus Lactis.
This study demonstrated that L. lactis may be used as a promising live delivery system for recombinant Sal k1 protein without altering its immunoreactivity; however, its efficacy in the context of the immune system is suggested to be pursued in future studies. PMID: 29757586 [PubMed - in process] (Source: Iranian Journal of Allergy, Asthma and Immunology)
Source: Iranian Journal of Allergy, Asthma and Immunology - April 1, 2018 Category: Allergy & Immunology Authors: Ghasemi Z, Varasteh AR, Moghadam M, Sedghi F, Jabbariazad F, Sankian M Tags: Iran J Allergy Asthma Immunol Source Type: research

Development and Evaluation of a Novel ELISA for Detection of Antibodies against HTLV-I Using Chimeric Peptides.
Abstract We aimed to develope a peptide-based indirect ELISA to detect antibodies against Human T-lymphotropic virus type I (HTLV-I). Two chimeric peptides (CP-1 and CP-2) were designed using linear immunodominant epitopes of gp-46-I, and gp21-I proteins, according to the sequence from Uniprot database. These peptides were studied initially in the ELISA using infected sera. The most promising peptideCP-1, was used to develop a peptide ELISA for detection of HTLV-I infected sera. The optimal conditions for CP-1ELISA were: the optimum coating buffer was 100mM NaHCO3, pH 9.6; coating peptide concentration was 10 &mic...
Source: Iranian Journal of Allergy, Asthma and Immunology - April 1, 2018 Category: Allergy & Immunology Authors: Mosadeghi P, Heydari-Zarnagh H Tags: Iran J Allergy Asthma Immunol Source Type: research

Association of Regulatory T Cells with Diabetes Type-1 and Its Renal and Vascular Complications Based on the Expression of Forkhead Box Protein P3 (FoxP3), Helios and Neurophilin-1.
Abstract In recent years, it has been recognized that regulatory T cells (Tregs) play a critical role in maintaining immune tolerance. Moreover, the expression of two markers named Helios and neurophilin-1 (NRP-1) has been highlighted in such cells. Helios, an intracellular transcription marker, largely differentiates twomost operative sub group of Tregs, namely naturally occurring (nTreg) and induced (iTreg) Tregs, and NRP-1 is reckoned as a membranous activity marker of Tregs. We aimed to count peripheral mononuclear cells expressing such markers in a group of type 1 diabetes patients to elucidate the possible r...
Source: Iranian Journal of Allergy, Asthma and Immunology - April 1, 2018 Category: Allergy & Immunology Authors: Khamechian T, Irandoust B, Mohammadi H, Nikoueinejad H, Akbari H Tags: Iran J Allergy Asthma Immunol Source Type: research

The Effect of Differentially Designed Fusion Proteins to Elicit Efficient Anti-human Thyroid Stimulating Hormone Immune Responses.
Abstract The production of human thyroid stimulating hormone (hTSH) immunoassays requires specific antibodies against hTSH which is a cumbersome process. Therefore, producing specific polyclonal antibodies against engineered recombinant fusion hTSH antigens would be of great significance. The best immunogenic region of the hTSH was selected based on in silico analyses and equipped with two different fusions. Standard methods were used for protein expression, purification, verification, structural evaluation, and immunizations of the white New Zealand rabbits. Ultimately, immunized serums were used for antibody tit...
Source: Iranian Journal of Allergy, Asthma and Immunology - April 1, 2018 Category: Allergy & Immunology Authors: Mard-Soltani M, Rasaee MJ, Khalili S, Sheikhi AK, Hedayati M, Ghaderi-Zefrehi H, Alasvand M Tags: Iran J Allergy Asthma Immunol Source Type: research

Imbalance of Peripheral Blood T Helper Type 17 Responses in Patients with Vitiligo.
Abstract There is growing evidence to suggest that Th cells play pivotal roles in a variety of chronic inflammatory diseases, including vitiligo. However, the exact role of different subsets of Th cells in the pathogenesis of vitiligo is still a question. The purpose of present study was to determine the mRNA expression level of Th17 master transcription factor retinoic acid receptor-related orphan receptors gamma (RORɣt) and cytokine mRNA and protein expression profiles of Th17 cells. 22 patients with vitiligo and 22 normal subjects were enrolled in the study. Gene expression profiles of freshly isolated periphe...
Source: Iranian Journal of Allergy, Asthma and Immunology - April 1, 2018 Category: Allergy & Immunology Authors: Behfarjam F, Mansouri P, Jadali Z Tags: Iran J Allergy Asthma Immunol Source Type: research

Evaluating Serum Levels of IL-33, IL-36, IL-37 and Gene Expression of IL-37 in Patients with Psoriasis Vulgaris.
Abstract Serum levels of interleukin (IL)-33, IL-36 and IL-37 have been reported to be up-regulated in various T helper (Th)1/Th17 mediated autoimmune/inflammatory diseases. Although IL-33 and IL-36 expression are increased in skin lesions of patients with psoriasis, their serum levels in such patients have not yet been adequately studied. We aimed to evaluate serum level of IL-33, IL-36 and IL-37 cytokines and IL-37 gene expression in patients with autoimmune/inflammatory disease of psoriasis and to explore their correlation with disease severity. Such evaluation further clarifies disease pathogenesis and may be ...
Source: Iranian Journal of Allergy, Asthma and Immunology - April 1, 2018 Category: Allergy & Immunology Authors: Sehat M, Talaei R, Dadgostar E, Nikoueinejad H, Akbari H Tags: Iran J Allergy Asthma Immunol Source Type: research

The Profile of Toll-like Receptor 2 (TLR2), TLR4 and Their Cytosolic Downstream Signaling Pathway in Common Variable Immunodeficiency (CVID) Patients.
Abstract Common variable immunodeficiency (CVID) is the most common clinical primary antibody deficiency, characterized by increased susceptibility to recurrent bacterial infections. Since Toll-like receptors (TLRs) play an important role in the maturation and differentiation of B-cells, TLRs' defect can be involved in the pathogenesis of CVID. Therefore, we evaluated the expression of TLR2 and TLR4 and their signaling pathway; also their association with autoimmunity, B-cell subtypes and response to pneumovax-23 were assessed in CVID patients. Sixteen CVID patients were enrolled in the study. Flow cytometry was u...
Source: Iranian Journal of Allergy, Asthma and Immunology - April 1, 2018 Category: Allergy & Immunology Authors: Sharifi L, Aghamohammadi A, Rezaei N, Yazdani R, Mahmoudi M, Amiri MM, Masoumi F, Bokaie S, Tavasolian P, Sanaei R, Moshiri M, Tavakolinia N, Alinia T, Azizi G, Mirshafiey A Tags: Iran J Allergy Asthma Immunol Source Type: research

Severe Combined Immunodeficiency: A Case Series and Review from a Tertiary Pediatric Hospital.
This study, we described 10 patients (6 male and 4 female) with SCID who were admitted to Mofid Children Hospital, Tehran, Iran, from 2006 to 2013. We reviewed patients' clinical manifestation, laboratory data, family history and outcome. The mean age at the time of diagnosis was 131.8 days. One patient had non-consanguineous parents. Seven patients received BCG vaccine before the diagnosis of SCID, three of them showed disseminated BCG infection. One patient presented with invasive pulmonary aspergillosis. Flow cytometric analysis showed T⁻B⁺NK⁻ in three patients, T⁻B⁻NK⁺ in five patients, T⁻B⁻NK⁻ in one...
Source: Iranian Journal of Allergy, Asthma and Immunology - April 1, 2018 Category: Allergy & Immunology Authors: Fallah S, Mesdaghi M, Mansouri M, Babaei D, Karimi A, Fahimzad SA, Armin S, Rafiei Tabatabaei S, Azma R, Khanbabaee G, Bashardoost B, Amirmoeini M, Sadr S, Jalilianhasanpour R, Ghanaei R, Rezaei N, Chavoshzadeh Z Tags: Iran J Allergy Asthma Immunol Source Type: research

Glucocorticoid Receptor Nuclear Translocation in CD4 T Cells from Severe and Moderate Asthmatic Patients Treated with Fluticasone/Vilanterol.
Abstract Different phenotypes of asthma from mild to severe are categorized based on diverse clinical features. A guideline for the recognition and treatment of asthma has been provided by Global Initiative for Asthma (GINA). To control symptoms and prevent asthma exacerbation in most patients combinational therapy with inhaled corticosteroids (ICS) and a long acting B2-adrenreceptor agonist (LABA) are recommended. Understanding asthma phenotypes would be helpful to improve asthma diagnosis and treatment. The aim of this study was to verify glucocorticoid receptor glcococorticoid receptor (GR) nuclear translocatio...
Source: Iranian Journal of Allergy, Asthma and Immunology - February 1, 2018 Category: Allergy & Immunology Authors: Alizadeh Z, Mazinani M, Mortaz E, Fazlollahi MR, Adcock I, Moein M Tags: Iran J Allergy Asthma Immunol Source Type: research

Regulatory Effects of Estradiol on Peripheral Blood Mononuclear Cells Activation in Patients with Asthma.
This study was designed to clarify whether estradiol may contribute to enhanced T helper (Th) 17-associated cytokines production by peripheral blood mononuclear cells (PBMC) in asthmatic patients and healthy individuals. PBMCs from patients with asthma and healthy donors were cultured with 17-β estradiol (E2) and phytohemagglutinin (PHA). The quantitative real-time polymerase chain reaction (qRT-PCR) was used to measure IL-6, IL-17, IL-23 and TGF-β. We observed a significant increased IL-17, IL-23 and TGF-β expression in PBMCs of patients compared to the healthy individuals. In addition, our findin...
Source: Iranian Journal of Allergy, Asthma and Immunology - February 1, 2018 Category: Allergy & Immunology Authors: Ahmadi-Vasmehjani A, Baharlou R, Atashzar MR, Raofi R, Jafari M, Razavi FS Tags: Iran J Allergy Asthma Immunol Source Type: research

Clinical Course and Factors Associated with Asthma Control in Children under Control-based Asthma Management: A Prospective Study.
Abstract In the current study, we sought to track the clinical course of children under control-based asthma management and focused on respiratory pathogens monitoring. We prospectively explored influencing factors for asthma control. 121 children with uncontrolled asthma between 3-14 years of age were recruited. Common respiratory pathogens were detected with pharyngeal swabs and serum aeroallergen-specific IgE was measured. Numeric asthma control scores, airway resistance and fractional concentrations of exhaled nitric oxide (FENO) were evaluated. A proper control-based asthma management plan was established by ...
Source: Iranian Journal of Allergy, Asthma and Immunology - February 1, 2018 Category: Allergy & Immunology Authors: Gao Q, Huang H, Zhu K, Liu X, Hou X, Guan H, Liu Y, Xin D, Xiang L, Shen K, Ni X Tags: Iran J Allergy Asthma Immunol Source Type: research

Effects of Connexin 43 Inhibition in an Ovalbumin-induced Mouse Model of Asthma.
Abstract Connexion 43 (Cx43), a gap junction protein, is expressed abundantly in the airway and has been implicated in the pathogenesis of asthma. However, the effects of blocking Cx43 in asthma remain unclear. We investigated the therapeutic effects of two specific Cx43 inhibitors (Gap26 and Gap27) on the development of allergic airway disease in mice. Allergic asthma was induced in BALB/c mice by sensitization and challenge with ovalbumin (OVA). Different doses of Cx43 inhibitors were administered by aerosol inhalation 1 h after OVA challenge on days 21 and 23. Airway hyperresponsiveness (AHR), lung pathology, m...
Source: Iranian Journal of Allergy, Asthma and Immunology - February 1, 2018 Category: Allergy & Immunology Authors: Huang JQ, Chen XY, Huang F, Fan JM, Shi XW, Ju YK Tags: Iran J Allergy Asthma Immunol Source Type: research

Regulatory Effect of Xiaoqinglong Decoction on Thymic Stromal Lymphopoietin (TSLP) Inflammation Promoter in Mice with Cold Asthma.
In this study, the role of the TSLP pathway in the onset of asthma and the protective mechanism of XQL were investigated. A total of 50 female mice were randomly divided into the following groups: the blank group (A), the model group (B), the XQL group (C), the dexamethasone group (D), and the XQL + dexamethasone group (E). Asthma was induced with ovalbumin, and corresponding drug intervention was carried out for 7 days, after which serum and lung tissue end points were analyzed. Serum interleukin 1β (IL-1β), tumor necrosis factor-α (TNF-α), nuclear factor κB (NF-κB), and TSLP levels ...
Source: Iranian Journal of Allergy, Asthma and Immunology - February 1, 2018 Category: Allergy & Immunology Authors: Song G, Zhang Y, Zhao K, Sun M, Cheng P, Wang J Tags: Iran J Allergy Asthma Immunol Source Type: research

Myeloid-derived Suppressor Cells Elimination by 5-Fluorouracil Increased Dendritic Cell-based Vaccine Function and Improved Immunity in Tumor Mice.
Abstract Myeloid-derived suppressor cells (MDSCs) are capable of suppressing the immune response. 5-Fluorouracil (5-FU) compared to other chemotherapy drugs have shown considerable decreases in the number of MDSCs without visible effects on T, B and natural killer cells, as well as dendritic cells (DCs). DC-based vaccines considered to be appropriate candidates for cancer immunotherapy. However, due to the presence of various factors like MDSCs in tumor microenvironment, DC vaccine cannot effectively perform its function. The purpose of this study was to evaluate the effect of low doses of 5-FU on the efficacy of ...
Source: Iranian Journal of Allergy, Asthma and Immunology - February 1, 2018 Category: Allergy & Immunology Authors: Khosravianfar N, Hadjati J, Namdar A, Boghozian R, Hafezi M, Ashourpour M, Kheshtchin N, Banitalebi M, Mirzaei R, Razavi SA Tags: Iran J Allergy Asthma Immunol Source Type: research

Indoleamine 2, 3-dioxygenase Up-regulates Hypoxia-inducible Factor-1 α Expression by Degrading L-tryptophan but Not Its Activity in Human Alloreactive T-cells.
Indoleamine 2, 3-dioxygenase Up-regulates Hypoxia-inducible Factor-1α Expression by Degrading L-tryptophan but Not Its Activity in Human Alloreactive T-cells. Iran J Allergy Asthma Immunol. 2018 Feb;17(1):56-67 Authors: Eleftheriadis T, Pissas G, Liakopoulos V, Stefanidis I Abstract Indoleamine 2, 3-dioxygenase (IDO) suppresses T-cell function at least in part by altering cell metabolism. Hypoxia-inducible factor-1 (HIF-1) increases upon T-cell activation and alters cell metabolism favoring their differentiation to effector cells. The effect of IDO on HIF-1α expression and activity was eva...
Source: Iranian Journal of Allergy, Asthma and Immunology - February 1, 2018 Category: Allergy & Immunology Authors: Eleftheriadis T, Pissas G, Liakopoulos V, Stefanidis I Tags: Iran J Allergy Asthma Immunol Source Type: research

Th1-Th17 Ratio as a New Insight in Rheumatoid Arthritis Disease.
Abstract The Th17, Th1 and dual Th17/Th1 cells are important players in rheumatoid arthritis (RA) disease. To assess their roles, the frequency and impact of these cells were investigated in patients with different disease activity. In 14 new cases and 41 established RA patients in comparison with 22 healthy controls, the percentages of Th17, Th1 and dual Th17/Th1 cells were determined by flow-cytometry and their correlations were investigated with disease activity score (DAS28). Moreover, serum levels of IL-6 and IL-17 as inducer and functional cytokines for Th17 were investigated. Finally, serum levels of anti c...
Source: Iranian Journal of Allergy, Asthma and Immunology - February 1, 2018 Category: Allergy & Immunology Authors: Bazzazi H, Aghaei M, Memarian A, Asgarian-Omran H, Behnampour N, Yazdani Y Tags: Iran J Allergy Asthma Immunol Source Type: research

The Correlation between the Numerical Status of Th22 Cells and Serum Level of IL-22 with Severity of Ulcerative Colitis.
This study aimed to evaluate the circulatory frequency of T-helper 22 (Th22), a subset of CD4+ T cells, and serum level of its signature cytokine, IL-22, in patients with UC. Blood samples from 30 patients with UC and 30 controls (n=30) were tested for IL-22 level and circulatory Th22-cell count by ELISA and Flow cytometric analysis, respectively. Our results revealed higher serum level of IL-22 as well as circulatory frequency of Th22 cells in patients with UC compared to those in healthy controls. Notably, effective factors on severity of the disease were age, Th22, IL-22, ESR and CRP. We conclude tha...
Source: Iranian Journal of Allergy, Asthma and Immunology - February 1, 2018 Category: Allergy & Immunology Authors: Arj A, Razavizadeh M, Mohammadi H, Nikoueinejad H, Akbari H Tags: Iran J Allergy Asthma Immunol Source Type: research

Good's Syndrome-Association of the Late Onset Combined Immunodeficiency with Thymoma: Review of Literature and Case Report.
Abstract Good's syndrome, the adult onset hypogammaglobulinemia associated with thymoma has been explained about six decades ago. It generally presents with recurrent infections and several paraneoplastic syndromes including myasthenia gravis, pure red cell aplasia, connective tissue disorders, superior vena cava, Horner's syndrome, lichen planus and inflammatory bowel disease. Lack of B cell, dysfunction of T cell, CD4+ T cell lymphopenia, reversed CD4/CD8+ T cell ratio, autoantibodies against Th17 related cytokines have been respected as the pathogenesis of the immune dysregulation this syndrome. A 57-year-old m...
Source: Iranian Journal of Allergy, Asthma and Immunology - February 1, 2018 Category: Allergy & Immunology Authors: Tavakol M, Mahdaviani SA, Ghaemi MR, Vaezi M, Dorudinia A, Jamaati H, Velayati AA Tags: Iran J Allergy Asthma Immunol Source Type: research

A Case of Anaphylaxis to Intramuscular but Not to Oral Application of Thiamine (Vitamin B1).
We report a 78 year-old non-atopic female with polyneuropathy who started to receive monthly intramuscular injections of thiamine hydrochloride. She had an anaphylaxis after the fourth injection. Skin prick test (SPT) with pure commercially available aqueous preparations was positive for thiamine hydrochloride. A titrated, single blinded, placebo-controlled oral provocation test with thiamine hydrochloride was well tolerated. The patient was then diagnosed as compartment allergy with hypersensitivity to parenteral but not to oral thiamine. Because in our patient, oral intake of thiamine has never been report...
Source: Iranian Journal of Allergy, Asthma and Immunology - February 1, 2018 Category: Allergy & Immunology Authors: Aurich S, Simon JC, Treudler R Tags: Iran J Allergy Asthma Immunol Source Type: research

Angioedema Due to Lamiaceae Allergy.
We present a 13-year-old male childallergic to three different plants (Salvia officinalis, Mentha piperita and Origanum onites L.) of Lamiaceae family. The patient developed angioedema 20-30 minutes after eating chicken meat with cheddar cheese. There was no history of allergy. Oral food challenge (OFC) with both cheddar cheese and chicken meat was negative. Skin tests for inhalant allergens were negative. 3 weeks later, the patient was admitted with angioedema after drinking sage tea. OFC with sage was applied and angioedema was observed. It was recognized that the first trigger, chicken meat...
Source: Iranian Journal of Allergy, Asthma and Immunology - February 1, 2018 Category: Allergy & Immunology Authors: Yazıcı S, Nacaroglu HT, Bahçeci Erdem S, Karaman S, Can D Tags: Iran J Allergy Asthma Immunol Source Type: research

Generation and Characterization of Siglec-F-Specific Monoclonal Antibodies.
Abstract Siglec-F (SF) is a surface glycoprotein expressed by mouse eosinophils and induces caspase- and mitochondria-dependent apoptosis after engagement with its cognate ligand or specific antibodies. This targeting eosinophils by monoclonal antibodies may help diverse diseases associated with increased frequency of eosinophils including allergy and asthma. In this paper, production of murine and rat monoclonal antibodies (mAbs) against Siglec-F has been addressed. Balb/c mice were immunized with siglec-F1 (SF1) and siglec-F2 (SF2) synthetic peptides conjugated to a carrier protein. Rats were immunized with Chin...
Source: Iranian Journal of Allergy, Asthma and Immunology - December 1, 2017 Category: Allergy & Immunology Authors: Shahmohammadi-Farid S, Ghods R, Jeddi-Tehrani M, Bayat AA, Mojtabavi N, Razavi A, Zarnani AH Tags: Iran J Allergy Asthma Immunol Source Type: research

Association Study of CD226 and CD247 Genes Single Nucleotide Polymorphisms in Iranian Patients with Systemic Sclerosis.
This study aimed to evaluate the possible association between CD226 and CD247 genes SNPs and risk of systemic sclerosis (SSc) in Iranian population. Study participants were 455 SSc patients and 455 age, sex and ethnic -matched healthy individuals. Genotyping of rs2056626 and rs763361 at CD247 and CD226 genes, respectively, was carried out using TaqMan MGB-based allelic discrimination real-time PCR. Neither alleles nor genotypes of both SNPs showed significant association with the risk of SSc. Furthermore, association analysis of the genotypes with clinical manifestations of the disease revealed that rs763361 variants were ...
Source: Iranian Journal of Allergy, Asthma and Immunology - December 1, 2017 Category: Allergy & Immunology Authors: Abbasi F, Mansouri R, Gharibdoost F, Aslani S, Mostafaei S, Kavosi H, Poursani S, Sobhani S, Mahmoudi M Tags: Iran J Allergy Asthma Immunol Source Type: research

Characteristics, Etiology and Treatment of Pediatric and Adult Anaphylaxis in Iran.
This study investigated 136 individuals, 64 males (47%) between 6 months and 68 years old, as well as 72 others (52.94%) under 18 years of age (pediatric). The following were the most common organs involved: Skin 86.02% (pediatric 91.66% vs adult 79.68%), respiratory tract 51.47% (pediatric 43.05% vs adult 60.93%), cardiovascular 50.73% (pediatric 54.16% vs adult 46.87%), gastrointestinal 20.58% (pediatric 27.7% vs adult 12.5% ) and neurologic system 5.88% (only in adults). The following were the most identified causing foods 69 (50.37%)[42 pediatric (children) and 27 adults], drugs 34( 25%)[14 pediatric and 20 adults], id...
Source: Iranian Journal of Allergy, Asthma and Immunology - December 1, 2017 Category: Allergy & Immunology Authors: Nabavi M, Lavavpour M, Arshi S, Bemanian MH, Esmaeilzadeh H, Molatefi R, Rekabi M, Ahmadian J, Eslami N, Shokri S, Darabi K, Sedighi GR, Fallahpour M Tags: Iran J Allergy Asthma Immunol Source Type: research

Association between Caregiver Exposure to Toxics during Pregnancy and Childhood-onset Asthma: A Case-control Study.
Abstract The aim of this study was to evaluate the associations between caregiver-reported use of medications, alcohol, cigarette and/or waterpipe (WP), and exposure to pesticides/detergents during pregnancy with childhood-onset asthma. The study design consisted of a case-control study, conducted between December 2015 and April 2016, recruited 1503 children, aged between 3-16 years old. A questionnaire assessed the sociodemographic characteristics (age, gender, education level of both parents), the family history of asthma, and other known risk factors of asthma (heating system at home, child history of recurrent...
Source: Iranian Journal of Allergy, Asthma and Immunology - December 1, 2017 Category: Allergy & Immunology Authors: Hallit S, Raherison C, Waked M, Salameh P Tags: Iran J Allergy Asthma Immunol Source Type: research

Attenuating Effect of Long-term Culture of Umbilical Cord Vein Mesenchymal Stromal Cells on Pulmonary Fibrosis in C57BL/6 Mice.
Abstract In recent studies, mesenchymal stromal cells (MSCs) have been increasingly employed to treat various diseases like pulmonary fibrosis (PF). There are very few MSCs in tissues so in order to obtain their sufficient numbers for therapeutic applications, their in vitro expansion is necessary. The aim of this study was to investigate the effects of long-term culture of the human umbilical cord vein MSCs (hUCV-MSCs) on pulmonary fibrosis in mice. MSCs were first isolated from human umbilical cord vein and cultured up to 18 passages. In C57BL/6 mice, 15 min after belomycin instillation, UCV-MSCs at passages (P)...
Source: Iranian Journal of Allergy, Asthma and Immunology - December 1, 2017 Category: Allergy & Immunology Authors: Moradi M, Rezaee MA, Mohammadi M, Rezaie MJ, Jalili A, Rahmani MR Tags: Iran J Allergy Asthma Immunol Source Type: research

Imbalance of Th17/Treg in the Pathogenesis of Mice with Paraquat-induced Acute Lung Injury.
Abstract Recent studies suggest that imbalances in the ratios of CD4+ T helper cell subsets, T helper-17 (Th17) and regulatory T (Treg) cells play a crucial role in the pathogenesis of acute lung injury (ALI). However, studies of the imbalance of Th17/Treg in paraquat (PQ)-induced ALI have not been reported. Therefore, we investigated whether the ratio of Th17/Treg cells in a mouse model of PQ-induced ALI contributes to pathogenesis of ALI. Male Kunming mice were randomly treated with saline (control group) or PQ (PQ-poisoned (PQP) group); mice were sacrificed at either 12 hours (PQP-12h) or 24 hours (PQP-24h and ...
Source: Iranian Journal of Allergy, Asthma and Immunology - December 1, 2017 Category: Allergy & Immunology Authors: Yang X, Zhang JH, Zhang JF, Lin H, Chen W, Xiang L, Li CQ Tags: Iran J Allergy Asthma Immunol Source Type: research

Studying the Serum as Well as Serous Level of IL-17 and IL-23 in Patients with Serous Otitis Media.
This study shows an elevated presence of IL-17 and IL-23, as pro inflammatory cytokines, in OME. These finding may represent the contribution of such cytokines in the pathogenesis of OME. Blocking such molecules may yield new non-surgical therapeutics. PMID: 29338158 [PubMed - in process] (Source: Iranian Journal of Allergy, Asthma and Immunology)
Source: Iranian Journal of Allergy, Asthma and Immunology - December 1, 2017 Category: Allergy & Immunology Authors: Yeghaneh Moghaddam A, Talaei R, Nikoueinejad H, Akbari H Tags: Iran J Allergy Asthma Immunol Source Type: research

Ovariectomy Modifies TH2, and TH17 Balance in BALB/C Allergic Mice.
RAAD Abstract Asthma is a chronic inflammation of the airways affecting over 300 million people worldwide. As in the autoimmune diseases, it is well described that women are the most affected by asthma. The higher number of women presenting this pathology suggests the involvement of female sex hormones in the construction of the allergic immune response. Female Balb / c mice were used for the experiments. Thirty-eight animals were separated into four groups: OVX-Ova; Sham-Ova; OVX-Sal; Sham-Sal. Then animals underwent acute allergic induction protocol by Ovalbulmin (OVA). Ovariectomized animals showed greater num...
Source: Iranian Journal of Allergy, Asthma and Immunology - December 1, 2017 Category: Allergy & Immunology Authors: Souza MPA, Lima FMDS, Muniz IP, Pereira ÍS, Sousa LRO, Galantini MPL, Santos DPD, Figueiredo TB, Silva RAAD Tags: Iran J Allergy Asthma Immunol Source Type: research

Higher Activities of Hepatic Versus Splenic CD8+ T Cells in Responses to Adoptive T Cell Therapy and Vaccination of B6 Mice with MHC Class-1 Binding Antigen.
Abstract The liver has unique microenvironment which is known to induce tolerance of cytolytic CD8+ T cells to hepatic and extra hepatic antigens, resulting in persistence of infection of the liver by the hepatitis B and C viruses. However, under some conditions, functional immune responses can be elicited in the liver in particular to show preferential retention of activated CD8+ T cells. It is not clear whether this retention depends on the type of the exogenous immunostimulatory or the endogenous innate immune cells. The T cell receptor (TCR) transgenic OT-1 (CD8+) mouse model was used in which OT-1 cells were ...
Source: Iranian Journal of Allergy, Asthma and Immunology - December 1, 2017 Category: Allergy & Immunology Authors: Salem ML, E El Naggar R, A El Naggar S, A Mobasher M, H Mahmoud M, Badr G Tags: Iran J Allergy Asthma Immunol Source Type: research

Effect of Periodontal Treatment on the Crevicular Level of High-mobility Group Box 1 and Soluble Triggering Receptor Expressed on Myeloid Cells 1 in Patients with Chronic Periodontitis.
Abstract The present study aimed to compare the levels of high-mobility group box 1(HMGB1) and soluble triggering receptor expressed on myeloid cells (sTREM1) in the gingival crevicular fluid (GCF). This cross-sectional cohort trial investigated two groups of 22 eligible chronic periodontitis and 22 periodontally healthy individuals (student volunteers) both before and after the periodontal treatment.  GCF was collected from the deepest pockets with clinical attachment loss≥3 mm. Both groups received oral hygiene instructions, and scaling and root planning were performed in the test group. Enzyme-linked im...
Source: Iranian Journal of Allergy, Asthma and Immunology - December 1, 2017 Category: Allergy & Immunology Authors: Paknejad M, Sattari M, Akbari S, Mehrfard A, Aslroosta H Tags: Iran J Allergy Asthma Immunol Source Type: research

Endobronchial Lesion in Eosinophilic Granulomatosis with Polyangiitis.
Abstract Eosinophilic granulomatosis with polyangiitis is a systemic vasculitis. It could affect respiratory system, kidney, and central nervous system frequently; however, all body organs could be involved. Asthma and eosinophilic pneumonia are predominant manifestations in respiratory system. Bronchoalveolar lavage or lung biopsy may be used for diagnosis, but endobronchial lesion is not considered as a manifestation of eosinophilic granulomatosis with polyangiitis. Here we present a case of eosinophilic granulomatosis with polyangiitis with unusual endobronchial lesion which was confirmed by endobronchial biops...
Source: Iranian Journal of Allergy, Asthma and Immunology - December 1, 2017 Category: Allergy & Immunology Authors: Chen GL, Wu CH, Perng WC Tags: Iran J Allergy Asthma Immunol Source Type: research

Kounis Syndrome Induced by Oral Intake of Diclofenac Potassium.
Abstract An acute coronary syndrome (ACS) occurring during the course of an allergic reaction is called Kounis syndrome (KS). The second case of KS induced by diclofenac potassium (DP) is presented in this report. A 67-year-old man was brought to our emergency department with the possible diagnosis of anaphylactic shock by the ambulance staff. It emerged that widespread erythema and pruritus developed after taking DP. Then, he lost consciousness. Diffuse urticarial lesions were detected on physical examination at the emergency department. He complained of chest pain during his observation, and progressive ST segme...
Source: Iranian Journal of Allergy, Asthma and Immunology - December 1, 2017 Category: Allergy & Immunology Authors: Gunes H, Turan Sonmez F, Saritas A, Koksal Y Tags: Iran J Allergy Asthma Immunol Source Type: research

Effect of Nebulized 3% Hypertonic Saline with Salbutamol on Management of Acute Asthma in Outpatient Adults: A Double-blind, Randomized Clinical Trial in Emergency Department.
This study aimed to compare the efficacy of 3% hypertonic saline (HS) plus salbutamol with solely salbutamol on management of acute adults' asthma based on peak flow meter findings. In this double-blind randomized clinical trial, 340 adult patients with acute asthma attacks admitted to emergency department of Ahvaz Golestan and Emam hospitals were enrolled during 2014-2015. The patients were allocated randomly to intervention group (nebulized 2.5 mg of salbutamol and 2.5 mL of 3% HS solution for three consecutive 20-min periods) and control group (nebulized only salbutamol in the same dose and time of the intervention grou...
Source: Iranian Journal of Allergy, Asthma and Immunology - October 1, 2017 Category: Allergy & Immunology Authors: Forouzan A, Masoumi K, Delirrooyfard A, Asgari Darian A, Mokhtar Gandomani L Tags: Iran J Allergy Asthma Immunol Source Type: research

Investigating the Association of ADAM33 Single Nucleotide Polymorphisms (SNPs) with Susceptibility to Allergic Asthma in Azerbaijan Population of Iran: A Case-control Study.
Abstract Asthma, affecting a growing number of populations, is a clinical condition with complex cellular and genetic factors. Single nucleotide polymorphisms (SNPs) in gene coding for molecules, which play major roles in the immunopathogenesis of asthma have been considered recently as genetic predisposing factors this disease. Possible association between two SNPs in a disintegrin and metalloprotease 33 (ADAM33), which participates in airway remodeling, and susceptibility to asthma was studied in this study. 190 patients with asthma and 180 healthy controls were enrolled in this case-control study. Using convent...
Source: Iranian Journal of Allergy, Asthma and Immunology - October 1, 2017 Category: Allergy & Immunology Authors: Zeinaly I, Sadeghi-Shabestrai M, Babaloo Z, Razavi A, Sajay-Asbaghi M, Sadigh-Eteghad S, Kazemi T Tags: Iran J Allergy Asthma Immunol Source Type: research

The Changes of Th17/Treg and Related Cytokines: IL-17, IL-23, IL-10, and TGF- β in Respiratory Syncytial Virus Bronchiolitis Rat Model.
The Changes of Th17/Treg and Related Cytokines: IL-17, IL-23, IL-10, and TGF-β in Respiratory Syncytial Virus Bronchiolitis Rat Model. Iran J Allergy Asthma Immunol. 2017 Oct;16(5):386-395 Authors: Gao M, Liu LX, Wu FL, Zhang X, Li YY, Shi T, Li DZ, Han TT Abstract Respiratory syncytial virus (RSV) is the leading cause of bronchiolitis and hospitalization that lead to high morbidity and mortality among young infants. T helper 17 (Th17) cells and regulatory T cells (Tregs) play essential roles in the pathogenesis of autoimmune, cancer, and inflammatory diseases. However, whether changes in T-cell ...
Source: Iranian Journal of Allergy, Asthma and Immunology - October 1, 2017 Category: Allergy & Immunology Authors: Gao M, Liu LX, Wu FL, Zhang X, Li YY, Shi T, Li DZ, Han TT Tags: Iran J Allergy Asthma Immunol Source Type: research

Increased Genes Expression Levels of Cytokines Related to Th17/Treg Cells in Peripheral Blood Mononuclear Cell Correlate with Clinical Severity in COPD and Mustard Gas-exposed Patients.
In this study, expression of cytokines genes levels related to Th17/Treg cells was determined in peripheral blood mononuclear (PBMC) of mustard lung patients and was compared with COPD patients and healthy controls (HC). Real time-polymerase chain reaction was used to assay genes expression levels of Th17 related cytokines (IL-17, IL-6 and TGF-β) and Treg related cytokines (IL-10, TGF-β). IL-17 gene expression level considerably was higher in SM patients (9.98±0.65, p
Source: Iranian Journal of Allergy, Asthma and Immunology - October 1, 2017 Category: Allergy & Immunology Authors: Farahani P, Halabian R, Vahedi E, Salimian J Tags: Iran J Allergy Asthma Immunol Source Type: research

Interleukin-37 Relieves Allergic Inflammation in a House Dust Mite Allergic Rhinitis Murine Model.
This study demonstrates that intranasal IL-37 can suppress Th2 and Th17 responses in an AR murine model. Furthermore, these data suggest that IL-10 is increased, but CD4+CD25+Foxp3+T cells are not correlated with the IL-37-induced mechanism. PMID: 29149780 [PubMed - in process] (Source: Iranian Journal of Allergy, Asthma and Immunology)
Source: Iranian Journal of Allergy, Asthma and Immunology - October 1, 2017 Category: Allergy & Immunology Authors: Kim DH, Kim SW, Kim SW, Kang JM Tags: Iran J Allergy Asthma Immunol Source Type: research

Macrophages from Behcet's Disease Patients Express Decreased Level of Aryl Hydrocarbon Receptor (AHR) mRNA.
In this study, we investigated the mRNA expression of AHR in the monocyte-derived and M1 macrophages in active BD patients in comparison to healthy controls. Isolated monocytes from 10 healthy controls and 10 active BD patients were differentiated to macrophages by macrophage-colony stimulating factor (M-CSF) for 7 days. Cells were then polarized to M1 macrophages by lipopolysaccharide (LPS) and interferon-γ (IFNγ) for 24h. Monocyte purity and macrophage markers expression were analyzed by flow cytometry. Analysis of AHR mRNA expression was performed by SYBR Green real-time PCR. Our results showed that AHR expr...
Source: Iranian Journal of Allergy, Asthma and Immunology - October 1, 2017 Category: Allergy & Immunology Authors: Palizgir MT, Akhtari M, Mahmoudi M, Mostafaei S, Rezaeimanesh A, Akhlaghi M, Shahram F Tags: Iran J Allergy Asthma Immunol Source Type: research

Frequency of Circulatory Regulatory Immune Cells in Iranian Patients with Type 1 Diabetes.
Abstract Type 1 diabetes (T1D) is the result of the autoimmune destruction of insulin-producing beta cells. Regulatory T cells (Tregs) and plasmacytoid dendritic cells (PDCs) act as mediators of peripheral tolerance. We investigated the possible alterations of such cells in peripheral blood of patients with T1D compared to normal individuals. This comparison may lead to a better understanding of the immunopathogenesis processes involved in T1D. 92 participants, including 49 patients with T1D and 43 healthy controls were studied. 3 mL of blood was taken from all participants. After isolating peripheral blood mononu...
Source: Iranian Journal of Allergy, Asthma and Immunology - October 1, 2017 Category: Allergy & Immunology Authors: Khamehchian T, Nemati E, Jazayeri M, Azimi S, Nikoueinejad H, Akbari H, Irandoust B Tags: Iran J Allergy Asthma Immunol Source Type: research