Methyltransferase-like 3 (METTL3) Epigenetically Modulates Glutathione Peroxidase 4 (GPX4) Expression to Affect Asthma
In this study, we delved into the regulatory mechanisms governing asthma, focusing on Methyltransferase-like 3 (METTL3). Through an ovalbumin (OVA)-induced mouse model and interleukin-13 (IL-13)-induced cell model, we mimicked the in vivo and in vitro functions of METTL3 in asthma. Our research revealed that METTL3 expression significantly decreased in asthma-induced mice and IL-13-stimulated cells compared to the control group. Moreover, METTL3 overexpression enhanced bronchial epithelial cell viability and proliferation. Mechanistically, we observed elevated levels of total iron, Fe2+, malondialdehyde (MDA), lipid reacti...
Source: Iranian Journal of Allergy, Asthma and Immunology - March 13, 2024 Category: Allergy & Immunology Authors: Liangfeng Lin Xiaohao Hu Qiaoyu Li Linlin Huang Source Type: research

Mesenchymal Stem Cell-derived Exosome; An Interesting Nanocarrier to Improve Allergen-specific Intranasal Immunotherapy
In conclusion, the Exo-OVA improved the INIT efficacy compared to free OVA. Therefore, this formulation could be introduced as an effective approach for immunomodulatory purposes with a shorter treatment duration and reduced side effects.PMID:38477953 | DOI:10.18502/ijaai.v22i6.14645 (Source: Iranian Journal of Allergy, Asthma and Immunology)
Source: Iranian Journal of Allergy, Asthma and Immunology - March 13, 2024 Category: Allergy & Immunology Authors: Sajad Dehnavi Maryam Dadmanesh Negin Hosseini Rouzbahani Mahmood Karimi Ali Asadirad Mohammad Gholami Khodayar Ghorban Source Type: research

Curcumin and Its Semisynthetic Derivative F-Curcumin Ameliorate the Expression of Cytokines in Autoimmune Encephalomyelitis Mouse Models of Multiple Sclerosis
In conclusion, curcumin and F-curcumin significantly modulate cytokine gene expression during EAE induction, potentially alleviating inflammation in MS, with F-curcumin showing a more substantial effect.PMID:38477954 | DOI:10.18502/ijaai.v22i6.14646 (Source: Iranian Journal of Allergy, Asthma and Immunology)
Source: Iranian Journal of Allergy, Asthma and Immunology - March 13, 2024 Category: Allergy & Immunology Authors: Saeid Khosropour Elahe Shahvarooghi Hossein Rezaeizadeh Maryam Esmaeelzadeh Source Type: research

Exploring the Impact of Leishmania Major on Mesenchymal Stem Cells: Evaluating Differentiation, and Immunomodulatory Function
In this study, we examined how Leishmania major (L. major) promastigotes affect the properties of MSCs. MSCs were isolated from adipose tissue and categorized into two groups: one group left untreated and the other group exposed to L. major. Giemsa staining was employed to accurately quantify the number of parasites that entered the cells. After 72 hours, real-time polymerase chain reaction was utilized to assess the expression of TLRs. Additionally, the flow cytometry technique was used to evaluate the expression of surface markers on the MSCs. Our results showed that MSCs can engulf parasites and increase the expression ...
Source: Iranian Journal of Allergy, Asthma and Immunology - March 13, 2024 Category: Allergy & Immunology Authors: Elham Mashayekh Arezou Khosrojerdi Ahmad Zavaran Hosseini Sara Soudi Source Type: research

Macrophage Activation Syndrome as a Complication of Chronic Granulomatous Disease: A Case Report
We describe a 10-month-old patient with a fatal secondary HLH as a CGD primary manifestation. We carried out an autopsy and found noncaseating granulomas, an aspergilloma in the lung, and hemophagocytosis. We performed a DHR assay on the patient's mother and grandmother, showing a bimodal pattern conclusive of X-linked CGD. Thus, our definitive diagnosis was CGD complicated by macrophage activation syndrome. CGD is caused by phagocytes' inability to control pathogens, resulting in granulomas. Secondary HLH is a severe complication and could be characterized by the proliferation of macrophages and T lymphocytes and the prod...
Source: Iranian Journal of Allergy, Asthma and Immunology - March 13, 2024 Category: Allergy & Immunology Authors: Eduardo Liquidano-Perez Mariana Carmona Berr ón Rosa Itzel Carrillo Nieto Celso Tom ás Corcuera Delgado Lizbeth Blancas Galicia Selma Cecilia Scheffler Mendoza Source Type: research

A Case of Insidious Onset of Kimura Disease-associated Immunoglobulin A Nephropathy without Eosinophil Infiltration in the Renal Tissue
In this study, we report a case of KD combined with immunoglobulin A nephropathy that first presented as a mass in the inguinal region, followed by recurrent renal involvement. Previous reports suggested that renal involvement caused by KD was due to direct infiltration of eosinophils; however, in this case, no eosinophil infiltration was found in the renal tissue after renal biopsy. This observation reminds us to approach the case from an immune-related molecular perspective to investigate the exact cause of renal damage due to KD.PMID:38477957 | DOI:10.18502/ijaai.v22i6.14649 (Source: Iranian Journal of Allergy, Asthma and Immunology)
Source: Iranian Journal of Allergy, Asthma and Immunology - March 13, 2024 Category: Allergy & Immunology Authors: Linxin Liu Haoxian Gou Yongfa Liu Shuai Hu Xiaoli Yang Bo Li Source Type: research

Macrophage Activation Syndrome Secondary to Histoplasmosis in an Adult Female Carrier of X-linked Chronic Granulomatous Disease with Extreme Lyonization
Iran J Allergy Asthma Immunol. 2023 Dec 28;22(6):609-612. doi: 10.18502/ijaai.v22i6.14650.ABSTRACTnot applicable to a Letter to the Editor.PMID:38477958 | DOI:10.18502/ijaai.v22i6.14650 (Source: Iranian Journal of Allergy, Asthma and Immunology)
Source: Iranian Journal of Allergy, Asthma and Immunology - March 13, 2024 Category: Allergy & Immunology Authors: Ximena Le ón-Lara Susana Garc ía-Pavón Osorio Sara Espinosa Padilla Lizbeth Blancas-Galicia Source Type: research

The Effect of Oral Montelukast in Controlling Asthma Attacks in Children: A Randomized Double-blind Placebo Control Study
This study was conducted as a double-blind placebo-controlled clinical trial on 80 children aged 1-14 years with asthma who were admitted to the emergency department of Bahrami Children's Hospital (Tehran, Iran) during one year. Patients were randomly divided into case and control groups. In addition to the standard asthma attack treatment, Montelukast was prescribed in the case group and placebo in the control group for one week. Patients were evaluated in terms of asthma attack severity score and oxygen saturation percentage (SpO2) in room air as primary outcomes 1, 4, 8, 24 and 48 hours after admission. In the first 48 ...
Source: Iranian Journal of Allergy, Asthma and Immunology - December 12, 2023 Category: Allergy & Immunology Authors: Mohsen Jafari Masoomeh Sobhani Kambiz Eftekhari Armen Malekiantaghi Mohammad Gharagozlou Alireza Shafiei Source Type: research

Chronic Allergen Exposure Contributes to Steroid Resistance via Increased Phosphorylation of Glucocorticoid Receptors S226 and p38 MAPK in a Mouse Model of Asthma
This study aims to investigate whether chronic allergen exposure can induce steroid resistance and whether it is associated with p38 MAPK activation in asthma. A mouse model of asthma was prepared by sensitizing and challenging mice with chronic ovalbumin (OVA) exposure. Key features of allergic asthma, encompassing bronchial hyperresponsiveness, pathology of lung tissues, cytokine profiles of inflammation in bronchoalveolar lavage fluid (BALF), and serum immunoglobulin (Ig)E concentration were evaluated. Furthermore, suppressive effects of corticosteroid on the splenocytes under stimulation of lipopolysaccharides, glucoco...
Source: Iranian Journal of Allergy, Asthma and Immunology - December 12, 2023 Category: Allergy & Immunology Authors: Yan Zhou Limin Zhou Weizong Jin Chenhui Qiu Hualiang Jin Source Type: research

Amygdalin Improves Allergic Asthma via the Thymic Stromal Lymphopoietin-dendritic Cell-OX40 Ligand Axis in a Mouse Model
Iran J Allergy Asthma Immunol. 2023 Oct 29;22(5):430-439. doi: 10.18502/ijaai.v22i5.13993.ABSTRACTAsthma, characterized by persistent inflammation and increased sensitivity of the airway, is the most common chronic condition among children. Novel, safe, and reliable treatment strategies are the focus of current research on pediatric asthma. Amygdalin, mainly present in bitter almonds, has anti-inflammatory and immunoregulatory potential, but its effect on asthma remains uninvestigated. Here, the impact of amygdalin on the thymic stromal lymphopoietin (TSLP)-dendritic cell (DC)-OX40L axis was investigated. A BALB/c mouse mo...
Source: Iranian Journal of Allergy, Asthma and Immunology - December 12, 2023 Category: Allergy & Immunology Authors: Wen Cui Huan Zhou Ya-Zun Liu Yan Yang Yi-Zhong Hu Zhao-Peng Han Jian-Er Yu Zheng Xue Source Type: research

MicroRNA-29a-3p Accelerates Inflammatory Damage in Neonatal Pneumonia Via Targeting Kr üppel-like Factor 4
This study demonstrates originally that miR-29a-3p boosts inflammatory damage in NP via targeting KLF4, offering a basis for clinically diagnosing and treating NP.PMID:38085146 | DOI:10.18502/ijaai.v22i5.13994 (Source: Iranian Journal of Allergy, Asthma and Immunology)
Source: Iranian Journal of Allergy, Asthma and Immunology - December 12, 2023 Category: Allergy & Immunology Authors: Xiao Juan Xu Wei Liu ShiNa Liland Source Type: research

Immunoregulatory Effect of Calcitriol on Experimental Autoimmune Encephalomyelitis (EAE) Mice
This study aimed to assess calcitriol's impact on EAE manifestations and cytokine profile in mice. In this study, we recruited twenty-eight C57BL/6 mice and divided them into 4 groups: healthy controls, EAE, EAE with calcitriol treatment, and healthy mice with calcitriol treatment. CD4+ T cells were isolated from splenocytes using magnetic-activated cell sorting. Real-time polymerase chain reaction was employed to quantify the genes associated with Th9 cells (i.e., SPI1 encoding PU.1 and IL9 encoding interleukin [IL]-9). Moreover, the levels of IL-17 and transforming growth factor beta (TGF-β) were evaluated through enzym...
Source: Iranian Journal of Allergy, Asthma and Immunology - December 12, 2023 Category: Allergy & Immunology Authors: Behrouz Robat-Jazi Mona Oraei Sama Bitarafan Seyed Alireza Mesbah-Namin Ali Noori-Zadeh Fatemeh Mansouri Karim Parastouei Ali Anissian Mir Saeed Yekaninejad Ali Akbar Saboor Yaraghi Source Type: research

Cycle Threshold Values Predict COVID-19 Severity and Mortality but Are not Correlated with Laboratory Markers
Iran J Allergy Asthma Immunol. 2023 Oct 29;22(5):468-481. doi: 10.18502/ijaai.v22i5.13996.ABSTRACTMany studies have evaluated the possible utility of cycle threshold (Ct) values as a predictor of Coronavirus disease 2019 (COVID-19) severity and patient outcome. Given the inconsistent results, we aimed to evaluate the association between severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Ct values and disease severity, inflammatory markers, and outcomes in Iranian patients with COVID-19. A retrospective study of 528 patients with COVID-19 hospitalized from September 2020 to October 2021 was conducted. Demographic,...
Source: Iranian Journal of Allergy, Asthma and Immunology - December 12, 2023 Category: Allergy & Immunology Authors: Behnaz Esmaeili Hoda Khoshnevis Atefe Alirezaee Abbas Shakoori Zahra Pourpak Hamid Chegini Zahra Ahmadinejad Source Type: research

Expression Analysis of Long Noncoding RNA-MALAT1 and Interleukin-6 in Inflammatory Bowel Disease Patients
Iran J Allergy Asthma Immunol. 2023 Oct 29;22(5):482-494. doi: 10.18502/ijaai.v22i5.13997.ABSTRACT Inflammatory bowel disease (IBD) manifests as chronic inflammation within the gastrointestinal tract. The study focuses on a long noncoding RNA (lncRNA) known as Metastasis-associated lung adenocarcinoma transcript 1 (MALAT1). MALAT1's misregulation has been linked with various autoimmune diseases and regulates proinflammatory cytokines. The role of IL6 in immune-triggered conditions, including IBD, is another focal point. In this research, the expression of MALAT1 and IL6 in IBD patients was meticulously analyzed to uncover ...
Source: Iranian Journal of Allergy, Asthma and Immunology - December 12, 2023 Category: Allergy & Immunology Authors: Mohsen Nemati Bajestan Moein Piroozkhah Vahid Chaleshi Naser Elmi Ghiasi Negar Jamshidi Reza Mirfakhraie Hedieh Balaii Shabnam Shahrokh Hamid Asadzadeh Aghdaei Zahra Salehi Ehsan Nazemalhosseini Mojarad Source Type: research

PD-1/PD-L1 Interaction Regulates BCL2, KI67, BAX, and CASP3, Altering Proliferation, Survival, and Apoptosis in Acute Myeloid Leukemia
This study aims to elucidate the effect of PD-L1 stimulation on the proliferation, survival, and apoptosis of acute myeloid leukemia (AML) cell lines. Two human AML cell lines, HL-60 and THP-1 were cultured and treated with phorbol 12-myristate 13-acetate (PMA) to induce PD-L1overexpression. Post-treatment PD-L1 expression was confirmed via flow cytometry. Subsequently, cell surface PD-L1 was stimulated using a recombinant PD-1, 24 hours post-PMA treatment. The expression alterations in pivotal genes including BCL2, MKI67, BAX, and CASP3 were monitored using quantitative real-time polymerase chain reaction 24 and 48 hours ...
Source: Iranian Journal of Allergy, Asthma and Immunology - December 12, 2023 Category: Allergy & Immunology Authors: Mojdeh Soltani Mahshid Vosoughi Mazdak Ganjalikhani-Hakemi Hoorieh Shapoorian Pezhman Beshkar Nahid Eskandari Behrooz Ghezelbash Source Type: research