Th9 Cells as a New Player in Inflammatory Skin Disorders.
Abstract CD4+T cells are composed of different subpopulations that differ in developmental pathways, surface markers, and their products. Among the catalog of these cells is Th9 cell subset that has a great capacity of Interleukin (IL)-9 production. They could be involved in the pathogenic or protective immune responses. Therefore, it is important to know how Th9 cells and cytokines influence the function of the human immune system as multitasking machinery, both in isolation or after the interaction with other surrounding cells. Since an important characteristic of Th9 cells is their tropism for skin, this articl...
Source: Iranian Journal of Allergy, Asthma and Immunology - April 1, 2019 Category: Allergy & Immunology Authors: Jadali Z Tags: Iran J Allergy Asthma Immunol Source Type: research

Recent Advances in Gene Therapy and Modeling of Chronic Granulomatous Disease.
Abstract The Chronic granulomatous disease (CGD) is a primary immunodeficiency that characterized by mutations in phagocyte nicotinamide adenine dinucleotide phosphate (NADPH) oxidase, resulting in deficient antimicrobial activity of phagocytic cells and recurrent childhood infections. Hematopoietic stem cell transplantation (HSCT) is a curative option for patients with human leukocyte antigen (HLA) matched donor, when conventional cares and therapies fail. However, in many cases when the patients have not an HLA-matched donor, they need to a method to recapitulate the function of the affected gene within the pati...
Source: Iranian Journal of Allergy, Asthma and Immunology - April 1, 2019 Category: Allergy & Immunology Authors: Jafarian A, Shokri G, Shokrollahi Barough M, Moin M, Pourpak Z, Soleimani M Tags: Iran J Allergy Asthma Immunol Source Type: research

Determining Laboratory Reference Values of TREC and KREC in Different Age Groups of Iranian Healthy Individuals.
In this study, we aimed to explore the effects of demographic variables including age, gender, weight, height and ethnicity on these two episomal DNA molecules. Second, for the first time in our country, we determined the reference values of TREC and KREC copy numbers in different age groups of Iranian healthy individuals as a threshold for identifying T cell and B cell lymphopenia. The TREC and KREC copy numbers were evaluated in 251 dried blood spot (DBS) samples from healthy volunteers (age range: 0-60 years). Six primary immunodeficiency (PID) patients were included as disease controls. TREC and KREC copies were m...
Source: Iranian Journal of Allergy, Asthma and Immunology - April 1, 2019 Category: Allergy & Immunology Authors: Shakerian L, Pourpak Z, Shamlou S, Domsgen E, Kazemnejad A, Dalili H, Nourizadeh M Tags: Iran J Allergy Asthma Immunol Source Type: research

High Glucose-reduced Apoptosis in Human Breast Cancer Cells Is Mediated by Activation of NF- κB.
In this study, MCF-7 derived from human breast adenocarcinoma, were cultured in DMEM with two different concentrations of glucose for 48 h (5.5 mM as normal glucose (NG) condition and 25 mM as HG condition). We used Zingiber officinale extraction for the inhibition of NF-κB. Cell proliferation assay was done by direct counting, cell viability by MTT method, bcl-2 by Immunocytochemistry, apoptosis by Hoechst/PI double staining and vascular endothelium growth factor (VEGF) by ELISA. Results showed that HG increased lactate production, significantly. HG increased cell proliferation, cell viability, VEGF secretion, and b...
Source: Iranian Journal of Allergy, Asthma and Immunology - April 1, 2019 Category: Allergy & Immunology Authors: Nasir Kansestani A, Mansouri K, Hemmati S, Zare ME, Moatafaei A Tags: Iran J Allergy Asthma Immunol Source Type: research

T Cell Subsets Profiling in Unexplained Infertile Women with Successful and Unsuccessful in Vitro Fertilization Outcome: Focus on the Effect of Seminal Plasma.
This study was conducted on 20 UI couples (ten with successful and ten with unsuccessful IVF outcome) and 10 fertile couples as the control group. Four color flow cytometry technique was used to detect Th cell subsets in the peripheral blood mononuclear cells (PBMC) with or without stimulation by SP. Results indicated that the frequencies of IL-17+ and Foxp3+ T cells after incubation with SP was significantly increased in couples with unsuccessful IVF outcome as compared to successful and healthy groups (p
Source: Iranian Journal of Allergy, Asthma and Immunology - April 1, 2019 Category: Allergy & Immunology Authors: Kanannejad Z, Namavar Jahromi B, Gharesi-Fard B Tags: Iran J Allergy Asthma Immunol Source Type: research

Downregulation of miR-542-3p Contributes to Apoptosis Resistance in Dermal Fibroblasts from Systemic Sclerosis Patients via Survivin Overexpression.
Abstract Systemic sclerosis (SSc) is characterized by excessive production of collagens by fibroblasts that leads to vast fibrosis. Resistance to apoptosis is one of the possible underlying mechanisms of fibrosis in these patients. Survivinis involved in inhibition of apoptosis and aberrantly functions in SSc. Since dysregulation of survivin-targeting microRNAs (miRNAs) has frequently been observed in cancer and some autoimmune disorders, this study aimed to investigate their expression status in dermal fibroblasts from SSc patients. DiffuseSSc patients were selected according to American College of Rheumatology c...
Source: Iranian Journal of Allergy, Asthma and Immunology - April 1, 2019 Category: Allergy & Immunology Authors: Vahidi Manesh P, Farazmand A, Gharibdoost F, Vanaki N, Mostafaei S, Kavosi H, Mahmoudi MB, Mahmoudi M Tags: Iran J Allergy Asthma Immunol Source Type: research

Gelatinases Increase in Bleomycin-induced Systemic Sclerosis Mouse Model.
Abstract Systemic sclerosis is a fibrotic autoimmune disease in which aberrant remodeling of the extracellular matrix in organs disturbs their functionalities. The aim of this study was to investigate the expression of gelatinases on systemic sclerosis. Consequently, a mouse model of systemic sclerosis was employed and the gelatinolytic activity of gelatinases was evaluated on the fibrotic tissues of this model. Two groups of ten mice were considered in this work: a group of systemic sclerosis model and control group. For the generation of systemic sclerosis model, mice received bleomycin, while the control group ...
Source: Iranian Journal of Allergy, Asthma and Immunology - April 1, 2019 Category: Allergy & Immunology Authors: Vafashoar F, Mousavizadeh K, Poormoghim H, Tavasoli A, Musavi Shabestari T, Javadmoosavi SA, Mojtabavi N Tags: Iran J Allergy Asthma Immunol Source Type: research

Chitin Micro Particles Regulate Splenocytes Immune Response in Experimental Autoimmune Encephalomyelitis.
Abstract Contrasting studies are reported on the induction of IL-10 and IFN-γ via chitin microparticles (CMPs) during immune stimulation. Our previous studies have shown marked protection among CMP treated Leishmania-infected mice via regulated IL-10/IFN-γ response, at the present study, once more, examined the inconsistent responses regarding the immunologic response of CMPS. To verify whether CMPs could indeed up-regulate IL-10/IFN-γ axis, isolated spleen cells from the myelin oligodendrocyte glycoprotein (MOG) induced experimental autoimmune encephalomyelitis (EAE) mice were cultured in the pr...
Source: Iranian Journal of Allergy, Asthma and Immunology - April 1, 2019 Category: Allergy & Immunology Authors: Mami S, Yeganeh F, Farahani E, Anissian A, Haji Molla Hoseini M Tags: Iran J Allergy Asthma Immunol Source Type: research

Evaluating Serum Levels of Pentraxin-3, von Willebrand Factor and C-X-C Motif Chemokine Ligand 13 as Inflammatory Markers of Unstable Angina Pectoris.
This study aimed at evaluating the diagnostic as well as prognostic value of serum inflammatory markers of pentraxin-3 (PTX-3), Von Willebrand Factor (vWf) and C-X-C Motif Chemokine Ligand 13 (CXCL13) in such patients. Out of sixty-nine patients, thirty-nine had USAP, thirty had stable angina pectoris (SAP), and thirty-nine were healthy controls. For all participants, serum PTX-3, vWf and CXCL13 levels were measured using ELISA. For each patient with USAP, the Thrombolysis in Myocardial Infarction (TIMI) and the scores of Global Registry of Acute Coronary Events (GRACE) were calculated to determine the severity of the dise...
Source: Iranian Journal of Allergy, Asthma and Immunology - April 1, 2019 Category: Allergy & Immunology Authors: Raygan F, Mohammadi H, Etminan A, Sehat M, Nikoueinejad H Tags: Iran J Allergy Asthma Immunol Source Type: research

Inhibition of Airway Contraction and Inflammation by Pomalidomide in a Male Wistar Rat Model of Ovalbumin-induced Asthma.
Abstract Asthma is a chronic inflammatory disease of the airways of the lungs. Pomalidomide (POM) a therapy for multiple myeloma has been stated to have an anti-inflammatory effect. The main goal of the present study was to assess its possible effect on airway contraction and inflammation in a rat model of ovalbumin-induced asthma. Different groups of rats received saline or pomalidomide (0.4, 0.8 mg/kg) or dexamethasone (0.6 mg/kg). The asthma was induced by ovalbumin (OVA). Trachea contraction was assayed by organ bath system. Airway histology was assessed using hematoxylin and eosin method. Serum Tumor necrosis...
Source: Iranian Journal of Allergy, Asthma and Immunology - April 1, 2019 Category: Allergy & Immunology Authors: Ghaderi M, Oryan S, Yousofvand N, Eidi A Tags: Iran J Allergy Asthma Immunol Source Type: research

Is the Inflammasome Pathway Active in the Peripheral Blood of Sulfur Mustard-exposed Patients?
This study aims to investigate the inflammasome pathway and their inflammatory cytokine (i.e IL-1 and IL-18) in the peripheral blood of mustard lung patients as well as chronic obstructive pulmonary disease (COPD) patients. This research was conducted as a cross-sectional analytical study on 15 SM patients and was compared with 15 COPD patients and 15 healthy controls. The real-time polymerase chain reaction was used to assess gene expression levels of inflammasome components (NLRP1, NLRP3, NLRC4, and ASC), inflammatory cytokines (IL-1β, IL-18, and IL-1βR), and IL-37 as an anti-inflammatory cytokine. Finally, the...
Source: Iranian Journal of Allergy, Asthma and Immunology - April 1, 2019 Category: Allergy & Immunology Authors: Etehad Asnaf S, Sabetghadam M, Jaafarinejad H, Halabian R, Parvin S, Vahedi E, Pazoki N, Salimian J Tags: Iran J Allergy Asthma Immunol Source Type: research

A Rare Case of Hyper IgE Syndrome with Vocal Cords Involvement.
Abstract Hyperimmunoglobulin E syndrome (HIGE) is considered as a phagocytic or a newly classified complex and heterogeneous primary immunodeficiency disease with symptoms such as increased levels of immunoglobulin E, eczema, and, recurrent lung and skin infections. In this paper, we have presented a rare case of this syndrome. A 9-year-old Iranian girl presented with a history of pruritic maculopapular rash who was eventually diagnosed as a case of HIGE. In her recent admission, she had dysphonia, stridor and huge cauliflower cutaneous lesions on her neck, finger and vocal cords, which did not respond to intraven...
Source: Iranian Journal of Allergy, Asthma and Immunology - April 1, 2019 Category: Allergy & Immunology Authors: Sharafian S, Movahedi M, Kalantari A, Parvaneh N, Gharagozlou M Tags: Iran J Allergy Asthma Immunol Source Type: research

The Review of in Vitro and in Vivo Studies over the Glycyrrhizic Acid as Natural Remedy Option for Treatment of Allergic Asthma.
Abstract Allergic asthma is the most common type of allergy which have become increasingly prevalent in all around the world. Airway eosinophilic inflammation is a major feature of allergic asthma. Glycyrrhiza uralensis (licorice) is one of the regular herbs in traditional Chinese medicine (TCM) as it has many effects on the immune system such as anti-inflammatory and immune regulatory activity; antiviral and antitumor effects. This review focuses on the "licorice" components, mainly glycyrrhizic acid (GA) and derivatives structure that evaluate its effects on the allergic asthma. We performed searching ...
Source: Iranian Journal of Allergy, Asthma and Immunology - February 1, 2019 Category: Allergy & Immunology Authors: Fouladi S, Masjedi M, Ganjalikhani Hakemi M, Eskandari N Tags: Iran J Allergy Asthma Immunol Source Type: research

The Stimulatory Effects of Medicinal Plants on β2-adrenoceptors of Tracheal Smooth Muscle.
The Stimulatory Effects of Medicinal Plants on β2-adrenoceptors of Tracheal Smooth Muscle. Iran J Allergy Asthma Immunol. 2019 Feb;18(1):12-26 Authors: Shakeri F, Ghorani V, Saadat S, Gholamnezhad Z, Boskabady MH Abstract Medicinal plants have been identified and used as primary sources in prevention and treatment of pulmonary diseases (mainly obstructive pulmonary diseases) from ancient times due to various pharmacological activities. In this review, the stimulatory effects of extracts, some fractions and constituents of medicinal plants on β2-adrenoceptors which could be used as possi...
Source: Iranian Journal of Allergy, Asthma and Immunology - February 1, 2019 Category: Allergy & Immunology Authors: Shakeri F, Ghorani V, Saadat S, Gholamnezhad Z, Boskabady MH Tags: Iran J Allergy Asthma Immunol Source Type: research

The Levels of Serum Biomarkers in Stable Asthma Patients with Comorbidities.
, Kirdar S, Karul A Abstract The effects of comorbidities on systemic inflammation markers in stable asthmatics and the consequences of such effects have not been well evaluated. We aimed to evaluate the effect of comorbidities on clinical manifestations and systemic inflammation in asthmatic patients under control. The study group consisted of asthmatic patients who applied to our pulmonology outpatient clinic and volunteered to participate. 120 clinically stable asthma patients (71 females and 49 males) and 35 healthy controls (19 females and 16 males) with similar age, gender, and body mass index distributions ...
Source: Iranian Journal of Allergy, Asthma and Immunology - February 1, 2019 Category: Allergy & Immunology Authors: Ceylan E, Bulut S, Yilmaz M, Örün H, Karadağ F, Ömürlü İK, Kirdar S, Karul A Tags: Iran J Allergy Asthma Immunol Source Type: research

Beneficial Effects of Hydroalcoholic Extract of Saffron in Alleviating Experimental Autoimmune Diabetes in C57bl/6 Mice.
This study has evaluated the effects of hydroalcoholic extract of saffron on the clinical and immunological profile of experimental autoimmune diabetes in C57BL/6 mice. After the establishment of the AD, mice were treated orally with hydroalcoholic extract of saffron (500 mg/kg) for 3 weeks. The results with p
Source: Iranian Journal of Allergy, Asthma and Immunology - February 1, 2019 Category: Allergy & Immunology Authors: Faridi S, Delirezh N, Abtahi Froushani SM Tags: Iran J Allergy Asthma Immunol Source Type: research

Effect of High-fat Diet on Tracheal Responsiveness to Methacholine and Insulin Resistance Index in Ovalbumin-sensitized Male and Female Rats.
Abstract Epidemiological and clinical studies have demonstrated a close association between obesity and asthma. The current study investigated the effect of high-fat diet on tracheal responsiveness to methacholine and insulin resistance in ovalbumin (OVA) sensitized male and female rats. The rats were divided into eight groups (n=6 per group): female with the normal diet (F+ND), male with the normal diet (M+ND), female OVA-sensitized with the normal diet (F+SND), male OVA-sensitized with the normal diet (M+SND), female with high-fat diet (F+HFD), male with high-fat diet (M+HFD), female OVA-sensitized with high-fat...
Source: Iranian Journal of Allergy, Asthma and Immunology - February 1, 2019 Category: Allergy & Immunology Authors: Ghobadi H, Alipour MR, Keyhanmanesh R, Boskabady MH, Aslani MR Tags: Iran J Allergy Asthma Immunol Source Type: research

Expression Levels of Predominant Adipokines and Activations of STAT3, STAT6 in an Experimental Mice Model of Obese Asthma.
Abstract Obese asthma is a new asthma phenotype. The underlying mechanisms are not clearly understood. Leptin and adiponectin are two predominant adipokines produced by adipose tissue. Studies have demonstrated a role of leptin on regulating the Janus kinase/signal transducer and ativator of transcription protein (JAK/STAT) signaling pathway and STAT3, STAT6 were known to have essential role on inflammatory cytokines production. However, whether STAT3 and STAT6 are activated and related to leptin merit further investigation. The aim of this study was to investigate the expression levels of leptin/adiponectin ratio...
Source: Iranian Journal of Allergy, Asthma and Immunology - February 1, 2019 Category: Allergy & Immunology Authors: Chong L, Liu L, Zhu L, Li H, Shao Y, Zhang H, Yu G Tags: Iran J Allergy Asthma Immunol Source Type: research

The Increase of pFAK and THBS1 Protein and Gene Expression Levels in Vascular Smooth Muscle Cells by Histamine-treated M1 Macrophages.
Abstract Atherosclerosis is developed due to the formation of atheroma plaques in the coronary arteries. In this process, M1 macrophages and vascular smooth muscle cells (VSMCs) are the main functional cells. Inflammatory mediators such as histamine may inflame M1 macrophages. The aim of this study was to determine the effect of M1 macrophage secretion contents on the gene and protein expression levels of focal adhesion kinase (FAK), vasodilator-stimulated phosphoprotein (VASP), and thrombospondin1 (THBS1). Whole blood samples from the six healthy subjects (stenosis70%) were prepared and peripheral blood mononucle...
Source: Iranian Journal of Allergy, Asthma and Immunology - February 1, 2019 Category: Allergy & Immunology Authors: Khosravi M, Najafi M, Amirfarhangi A, Karimi M, Fattahi F, Shabani M Tags: Iran J Allergy Asthma Immunol Source Type: research

The Anti-tumoral Effect of β-D-Mannuronic Acid (M2000) as a Novel NSAID on Treg Cells Frequency and MMP-2, MMP-9, CCL22 and TGFβ1 Gene Expression in Pre-surgical Breast Cancer Patients.
The Anti-tumoral Effect of β-D-Mannuronic Acid (M2000) as a Novel NSAID on Treg Cells Frequency and MMP-2, MMP-9, CCL22 and TGFβ1 Gene Expression in Pre-surgical Breast Cancer Patients. Iran J Allergy Asthma Immunol. 2019 Feb;18(1):80-90 Authors: Kashefi S, Ahmadi H, Omranipour R, Mahmoodzadeh H, Jafarnezhad-Ansariha F, Tofighi Zavareh F, Mirshafiey A Abstract With respect to the role of chronic inflammation in the induction and progression of breast cancer (BC). The relationship between tumor and tumor microenvironment may be a hopeful strategy for BC therapy. According to the effect of &be...
Source: Iranian Journal of Allergy, Asthma and Immunology - February 1, 2019 Category: Allergy & Immunology Authors: Kashefi S, Ahmadi H, Omranipour R, Mahmoodzadeh H, Jafarnezhad-Ansariha F, Tofighi Zavareh F, Mirshafiey A Tags: Iran J Allergy Asthma Immunol Source Type: research

Association between Interleukin-32 and Interleukin-17A Single Nucleotide Polymorphisms and Serum Levels with Polycystic Ovary Syndrome.
In conclusion, IL-17A and IL-32 SNPs might be associated with predisposition to PCOS in Iranian women. PMID: 30848577 [PubMed - in process] (Source: Iranian Journal of Allergy, Asthma and Immunology)
Source: Iranian Journal of Allergy, Asthma and Immunology - February 1, 2019 Category: Allergy & Immunology Authors: Hesampour F, Namavar Jahromi B, Tahmasebi F, Gharesi-Fard B Tags: Iran J Allergy Asthma Immunol Source Type: research

The Role of Progesterone in Cellular Apoptosis of Skin and Lung in a Bleomycin-injured Mouse Model.
The objective of this study was to examine the effect of progesterone on cellular apoptosis of skin and lung tissues in a mouse model of scleroderma. Four group of mice were involved in this study with 10 mice in each. The fibrotic model was induced by daily subcutaneous injection of bleomycin for 28 days. One week after initiation of fibrosis induction, mice received subcutaneous progesterone alone or with bleomycin for 21 days. Control group received only Phosphate buffered saline PBS. After 28 days, under lethal anesthesia skin and lung tissues were harvested for histological assessment and hydroxyproline measurement. A...
Source: Iranian Journal of Allergy, Asthma and Immunology - February 1, 2019 Category: Allergy & Immunology Authors: Vafashoar F, Poormoghim H, Mousavizadeh K, Mousavi Shabestari T, Tavasoli A, Javadmoosavi SA, Mojtabavi N Tags: Iran J Allergy Asthma Immunol Source Type: research

Altered Expression of miR-326 in T Cell-derived Exosomes of Patients with Relapsing-remitting Multiple Sclerosis.
Abstract  Invasion of auto-reactive CD4+ T cells especially Th17 into central nervous system (CNS) is an underlying pathogenic mechanism in multiple sclerosis (MS). CD4+ T cells release exosomes which are enriched in microRNAs, reflective of cell's physiological or pathological condition. Thus exosomes could be potent agents to provide quantitative and qualitative information about involved cells in MS. We investigated the expression of pathogenic microRNAs in T cells-derived exosomes of MS patients or healthy controls. Conventional T cells (Tconv) derived from relapsing-remitting (RR) MS patients (n=10) and ...
Source: Iranian Journal of Allergy, Asthma and Immunology - February 1, 2019 Category: Allergy & Immunology Authors: Azimi M, Ghabaee M, Naser Moghadasi A, Izad M Tags: Iran J Allergy Asthma Immunol Source Type: research

Immunomodulatory Effects of Human Adipose Tissue-derived Mesenchymal Stem Cells on T Cell Subsets in Patients with Rheumatoid Arthritis.
This study was designed to investigate the effects of co-cultured Ad-MSCs on peripheral blood mononuclear cells (PBMCs) of RA patients and healthy individuals, through assessing transcription factors of T cell subsets. PBMCs from RA patients and healthy donors were co-cultured with Ad-MSCs with or without Phytohaemagglutinin (PHA). The quantitative real-time polymerase chain reaction (qRT-PCR) was used to measure the expression of T-box 21 (T-bet), GATA-binding protein-3 (GATA3), retinoid-related orphan receptor γt (ROR-γt) and forkhead box P3 (Foxp3). Based on the results, Ad-MSCs greatly upregulated...
Source: Iranian Journal of Allergy, Asthma and Immunology - February 1, 2019 Category: Allergy & Immunology Authors: Baharlou R, Rashidi N, Ahmadi-Vasmehjani A, Khoubyari M, Sheikh M, Erfanian S Tags: Iran J Allergy Asthma Immunol Source Type: research

Induction of Immune Response and Protective Immunity by a Local Isolated Varicella Virus in Animal Model: A Future Candidates for Vaccine Production.
Abstract Preparation of the indigenous varicella zoster vaccine could significantly reduce the disease burden of varicella zoster virus especially in immunosuppressed children. To achieve this goal, the varicella zoster virus was isolated from an 8 years boy infected with chicken pox. The virus was cultivated in sensitive cell line and determined varicella zoster. The adaptation and attenuation of virus was carried out after several passages in MRC-5 cell culture, Primary Guinea pig embryo fibroblast cell culture and again switching in MRC-5 cell culture. The challenged of vaccine dose was found 3LogCCID50. Follow...
Source: Iranian Journal of Allergy, Asthma and Immunology - December 31, 2018 Category: Allergy & Immunology Authors: Esna-Ashari F, Sabahi F, Ravanshad M, Mohammadi A Tags: Iran J Allergy Asthma Immunol Source Type: research

Expression Analysis of BDNF Gene and BDNF-AS Long Noncoding RNA in Whole Blood Samples of Multiple Sclerosis Patients: Not Always a Negative Correlation between Them.
Abstract Multiple sclerosis (MS) is a chronic inflammatory disorder of the central nervous system (CNS), in which axonal damage is a deteriorative factor. Brain-Derived Neurotrophic Factor (BDNF) is described as a neuronal-survival gene, also capable of exerting pleiotropic effects on the immune cells. Here, we aimed to investigate expression levels of BDNF and its antisense RNA, BDNF-AS, in Iranian MS patients. Our case-control study was based on collecting 50 whole blood samples of relapsing-remitting MS patients and 50 healthy controls. Then, expression analysis of BDNF and BDNF-AS was performed by Real-time qu...
Source: Iranian Journal of Allergy, Asthma and Immunology - December 4, 2018 Category: Allergy & Immunology Authors: Gharzi V, Gangi M, Sayad A, Mazdeh M, Arsang-Jang S, Taheri M Tags: Iran J Allergy Asthma Immunol Source Type: research

MHC Class II Deficiency with Normal CD4+ T Cell Counts: A Case Report.
Abstract Major histocompatibility complex (MHC) class II deficiency is a rare primary immunodeficiency disorder (PID) with less than 200 cases worldwide. Here, we report an 8 month-old girl with MHC class II deficiency with a novel homozygous mutation in RFXANK gene (NM_001278728: exon 5: c.495G>A: p.Trp165*) and normal CD4+ T cell counts, diagnosed by whole exome sequencing (WES) and negative HLA-DR proteins on peripheral blood mononuclear cell (PBMC) in flow cytometry. She was referred with pneumonia, prolonged fever, resistance to antibiotics (ceftriaxone, clindamycin, and vancomycin), and low serum immunogl...
Source: Iranian Journal of Allergy, Asthma and Immunology - December 4, 2018 Category: Allergy & Immunology Authors: Abolnezhadian F, Saeedi-Boroujeni A, Iranparast S Tags: Iran J Allergy Asthma Immunol Source Type: research

Demographic, Clinical, and Allergic Characteristics of Children with Eosinophilic Esophagitis in Isfahan, Iran.
Abstract Eosinophilic esophagitis (EoE) is a chronic immune-mediated disease isolated to the esophagus Food allergy is thought to play an important role in the pathophysiology of EOE. The aim of this study is to evaluate demographic features and sensitivity of patients with reference to common food allergens. Children with documented EoE were enlisted for this study. Skin prick test and patch test were done for all participants. Each test contained common food allergens. Other tests, such as evaluation of total IgE and eosinophil count, were also done. A total of 30 patients (the mean age of participants was 3&plu...
Source: Iranian Journal of Allergy, Asthma and Immunology - December 3, 2018 Category: Allergy & Immunology Authors: Momen T, Saneian H, Amini N Tags: Iran J Allergy Asthma Immunol Source Type: research

Increased Circulating T Follicular Helper Cells in Iranian Children with Type I Diabetes.
In this study, we examined the frequency of circulating CD4+CXCR5+ and CD4+CXCR5+ICOS+ (representing Tfh) cells as well as serum levels of anti-glutamic acid decarboxylase 65 (GAD65) and islet cell autoantibodies (ICA) in children with type I diabetes. We analyzed the percentage of Tfh cells within peripheral blood mononuclear cells in 20 children with T1D (≤300 days from disease onset; Mean age 6.8±4.6 years) and 18 healthy individuals (Mean age 8.8±2.2 years) using flow cytometry. Anti-glutamic acid decarboxylase (GAD) and islet-cell cytoplasmic autoantibodies (ICA) levels were determined by ELISA and in...
Source: Iranian Journal of Allergy, Asthma and Immunology - December 3, 2018 Category: Allergy & Immunology Authors: Arab M, Razzaghy-Azar M, Salehi Z, Keshavarz M, Nasli-Esfahani E, Shekarabi M, Izad M Tags: Iran J Allergy Asthma Immunol Source Type: research

CbpM and CbpG of Streptococcus Pneumoniae Elicit a High Protection in Mice Challenged with a Serotype 19F Pneumococcus.
This study aims to determine the immunogenicity of CbpM, CbpG and CbpL proteins of Streptococcus pneumoniae in a mice model. The genes were cloned into pET21a expression vector and the recombinant proteins were produced. Mice were immunized with the purified recombinant proteins. Subsequently, the mice were challenged with S. pneumoniae ATCC 49619 (2×106 CFU) and their survival and bacterial clearances were followed 24 hours after infection. The antibody responses of the mice were determined by ELISA assay. The opsonophagocytosis assay was performed using rabbit's sera. Passive immunization was carried out using two ...
Source: Iranian Journal of Allergy, Asthma and Immunology - December 3, 2018 Category: Allergy & Immunology Authors: Kazemian H, Afshar D, Garcia E, Pourmand MR, Jeddi-Tehrani M, Aminharati F, Shokri F, Yazdi MH Tags: Iran J Allergy Asthma Immunol Source Type: research

Direct Costs of Asthma in a Referral Public Children's Hospital in Tehran, Iran.
Abstract Asthma is one of the most common chronic diseases all over the world, which impacts socioeconomics of families and health care systems. We need to estimate the costs of asthma to set health policies and structural adjustments to control burden of asthma in societies. The aim of present study was to evaluate direct costs of asthma in children in Tehran-Iran. In a descriptive-analytical study, 100 children who were referred to Children's Medical Center of Tehran University of Medical sciences, from March 2014 to March 2015, were selected randomly and studied. In an interview with parents we evaluated direct...
Source: Iranian Journal of Allergy, Asthma and Immunology - December 3, 2018 Category: Allergy & Immunology Authors: Rostamzadeh N, Akbari Sari A, Gharagozlou M Tags: Iran J Allergy Asthma Immunol Source Type: research

Elevated Expression of Tim-3 and PD-1 Immune Checkpoint Receptors on T-CD4+ Lymphocytes of Patients with Asthma.
Abstract Asthma is a chronic disorder of the airways characterized by reversible airflow obstruction, inflammation and bronchial hyperresponsiveness. Different immune cells and molecules have been attributed to involve in pathogenesis of asthma. In the current case-control study, the expression of T cell Ig and mucin domain-containing molecule-3 (Tim-3) and programmed death-1 (PD-1) was studied on CD4+ T cells of patients with asthma and normal controls. The frequency of Tim-3+/PD-1+/CD4+ T cells was determined by a three color flow cytometry method in 37 patients with asthma and 32 healthy controls. To evaluate t...
Source: Iranian Journal of Allergy, Asthma and Immunology - December 2, 2018 Category: Allergy & Immunology Authors: Mosayebian A, Koohini Z, Hossein-Nataj H, Abediankenari S, Abedi S, Asgarian-Omran H Tags: Iran J Allergy Asthma Immunol Source Type: research

Altered Frequencies of CD4+ CD25+ Foxp3+ and CD8+ CD25+ Foxp3+ Regulatory T Cells in Pre-eclampsia.
Abstract Regulatory T cells are of utmost importance for tolerating the fetus. In some pregnancy complications such as pre-eclampsia, the frequency of CD4+CD25+Foxp3+ regulatory T cells is altered, but there is no consistency regarding the results. Besides, little is known about the frequency of CD8+CD25+Foxp3+ Treg cells in pregnancy complications. Therefore, we aimed to investigate the frequency of both CD4+ and CD8+ regulatory T cells in the peripheral blood of women afflicted by preeclampsia. Ten non-pregnant, ten healthy pregnant, and ten preeclamptic women participated in this study. Four colors flow cytomet...
Source: Iranian Journal of Allergy, Asthma and Immunology - December 2, 2018 Category: Allergy & Immunology Authors: Zare M, Doroudchi M, Gharesi-Fard B Tags: Iran J Allergy Asthma Immunol Source Type: research

Adenovirus-mediated Gene Therapy for Allergy.
Abstract Allergy poses a heavy health burden in modern society. Other than symptom-relieving medications, the only available treatment approach is allergen-specific immunotherapy, which in spite of offering a potential cure, requires a long treatment duration with multiple doses of allergen administration and carries a risk of anaphylaxis. Gene therapy has shown advantages in experimental studies for treatment of tumors, genetic diseases, chronic infections, and allergy. To date, adenovirus has been the most extensively used gene transfer vector, and offers high efficiency and safety. Here, we review studies of ad...
Source: Iranian Journal of Allergy, Asthma and Immunology - December 1, 2018 Category: Allergy & Immunology Authors: Ma S, Guan J Tags: Iran J Allergy Asthma Immunol Source Type: research

Investigating the Association of Orosomucoid 1-like 3 (ORMDL3) Gene Polymorphism (rs12603332) with Susceptibility to Allergic Asthma in Iranian Northwestern Azeri Population.
In this study, we aimed to investigate the possible association of ORMDL3 single nucleotide polymorphism (SNP) (rs12603332) with susceptibility to allergic asthma in Iranian Northwestern Azeri population. 193 asthmatic patients and 185 normal individuals were included. Genomic DNA was extracted and genotyping was performed by standard restriction fragment length polymorphism-polymerase chain reaction RFLP-PCR method using BstUI restriction enzyme. Our results showed dominant presence of TC genotype and C allele in both patients (49.2% and 59.8%, respectively) and controls (48.6% and 60%, respectively). Frequency of genotyp...
Source: Iranian Journal of Allergy, Asthma and Immunology - December 1, 2018 Category: Allergy & Immunology Authors: Zeinaly I, Vossoughi N, Elieh Ali Komi D, Kazemi T, Babaloo Z, Razavi A, Sajay-Asbaghi M, Sadeghi-Shabestari M Tags: Iran J Allergy Asthma Immunol Source Type: research

Carbenoxolone Could Deteriorate Streptozotocin-induced Diabetes through Induction of Heat Shock Protein 70 and IFN- γ in C57BL/6 Mice.
Carbenoxolone Could Deteriorate Streptozotocin-induced Diabetes through Induction of Heat Shock Protein 70 and IFN-γ in C57BL/6 Mice. Iran J Allergy Asthma Immunol. 2018 Dec 01;17(6):564-573 Authors: Rasouli M, Jafari-Khataylou Y, Ashrafi-Helan J Abstract Type 1 diabetes (T1D), a spontaneous autoimmune disease, is associated with destruction of insulin-producing β-cells in the pancreas. Since some heat shock proteins (HSP), such as HSP70 exert a protective effect in both tissues and cells, the present study was conducted to elucidate the effects of carbenoxolone (CBX) as an HSP70 inducer on...
Source: Iranian Journal of Allergy, Asthma and Immunology - December 1, 2018 Category: Allergy & Immunology Authors: Rasouli M, Jafari-Khataylou Y, Ashrafi-Helan J Tags: Iran J Allergy Asthma Immunol Source Type: research

Evaluation of ERAP1 Gene Single Nucleotide Polymorphism in Impressing the Inflammatory Cytokine Profile of Ankylosing Spondylitis Patients.
This study replicated the association of polymorphisms in ERAP1 gene with the risk of AS in a population from Iranian. However, it did not directly determine the inflammatory profile of the AS patients. PMID: 30518188 [PubMed - in process] (Source: Iranian Journal of Allergy, Asthma and Immunology)
Source: Iranian Journal of Allergy, Asthma and Immunology - October 20, 2018 Category: Allergy & Immunology Authors: Mohammadi H, Babaie F, Hemmatzadeh M, Azizi G, Hajaliloo M, Ebrahimi AA, Kazemi T, Yousefi M, Rezaiemanesh A, Safarzadeh E, Baghbani E, Majidi J, Baradaran B Tags: Iran J Allergy Asthma Immunol Source Type: research

Effect of Progesterone on Expression of MMP7 and MMP13 in Lungs of Female Mice.
In this study we investigated the impact of pregnancy doses of progesterone hormone on the expression of a couple of matrix metalloproteinase (MMPs), which are known to be involved in tissue remodeling of lungs in health and disease, namely MMP7 and 13. Pregnancy maintenance dose of progesterone was administered to female BALB/c mice for 21 and 28 days, the control group received PBS for the same days. After removal of the lungs and RNA extraction, quantitative real-time PCR was done using specific primers for MMP7 and MMP13. We found that progesterone can slightly (not significantly) decrease the expression of MMP13 ...
Source: Iranian Journal of Allergy, Asthma and Immunology - October 20, 2018 Category: Allergy & Immunology Authors: Izadi E, Vafashoar F, Jorbozedar P, Safari P, Assarehzadegan MA, Poormoghim H, Kuhpayezadeh J, Mojtabavi N Tags: Iran J Allergy Asthma Immunol Source Type: research

Effects of Viola tricolor Flower Hydroethanolic Extract on Lung Inflammation in a Mouse Model of Chronic Asthma.
Abstract Asthma is a chronic inflammatory disease of the lungs driven by T cell activation. Viola tricolor L. as a traditional medical herb could suppress activated T lymphocytes and has been used empirically for asthma remedy. In the present study, we investigated the anti-inflammatory effect of Viola tricolor and its underlying mechanism on asthma characteristics induced by ovalbumin (OVA) in mice. BALB/c mice were randomly divided into six groups: normal control, Ovalbumin (OVA) control, OVA mice treated with Viola tricolor (50, 100 and 200 mg/kg) and dexamethasone (3 mg/kg). All mice except normal controls wer...
Source: Iranian Journal of Allergy, Asthma and Immunology - October 14, 2018 Category: Allergy & Immunology Authors: Harati E, Bahrami M, Razavi A, Kamalinejad M, Mohammadian M, Rastegar T, Sadeghipour HR Tags: Iran J Allergy Asthma Immunol Source Type: research

Effect of Kaempferol on Cyclooxygenase 2 (Cox2) and Cytosolic Phospholipase A2 (cPLA2) Protein Expression in BALB/c Mice.
The objective of this study was to investigate whether kaempferol could reduce omega-6 and ovalbumin-mediated allergic reactions at lung and trachea in BALB/c mice. Mice were allocated into five groups: 1) control group (CON); 2) positive control group with orally administration of omega-6 (POS); 3) bovine serum albumin (BSA) sensitization group (with BSA injection and ovalbumin inhalation); 4) BSA+K10 group: BSA injection, 10 μg/g of kaempferol administration and ovalbumin inhalation; and 5) BSA+K20 group: BSA injection, 20 μg/g of kaempferol administration and ovalbumin inhalation. Results revealed that serum hista...
Source: Iranian Journal of Allergy, Asthma and Immunology - October 14, 2018 Category: Allergy & Immunology Authors: Kang DR, Belal SA, Choe HS, Shin DK, Shim KS Tags: Iran J Allergy Asthma Immunol Source Type: research

Assessment of the Inhibitory Effects of Ficin-hydrolyzed Gelatin Derived from Squid (Uroteuthis duvauceli) on Breast Cancer Cell Lines and Animal Model.
Abstract Marine novel natural products have been applied for cancer therapy. Enzyme-digested gelatin hydrolysates have proven to serve as promising sources of potent biologically active peptides. Potential anti-breast cancer properties of the extracted Ficin-digesterd gelatin hydrolysate from Indian squid (Uroteuthis duvauceli) was extensively characterized by cellular and animal models. Gelatin was extracted from squid skin, hydrolyzed by Ficin, and characterized by standard physico-chemical methods. Ficin-digested gelatin hydrolysate was used at various doses of 0-0.1 mg/mL for assessment of MCF-7 and MDA-MB-231...
Source: Iranian Journal of Allergy, Asthma and Immunology - October 14, 2018 Category: Allergy & Immunology Authors: Shahidi S, Jamili S, Ghavam Mostafavi P, Rezaie S, Khorramizadeh M Tags: Iran J Allergy Asthma Immunol Source Type: research

Evaluation of IL-17 Producing Memory Regulatory and Effector T Cells Expressing CD26 Molecule in Patients with Psoriasis.
In this study, we aimed to analyze IL-17 producing Treg cells using CD26. Memory T cells were isolated from 10 patients with psoriasis and 10 controls. Ex vivo stimulated IL-17 producing regulatory (Forkhead Box P3 (Foxp3)+CD25+CD26-/low) and effector (Foxp3+CD25+CD26hi) memory T cells were analyzed by flow cytometry. IL-23, IL-6, TNFα, TGFβ and IL-17 cytokine levels were also evaluated. No significant difference in IL-17+memory regulatory T cells was seen between patients and controls (p=0.19). A significant decrease in the percentage of IL-17 producing CD26hi effector memory T cells was observed in patien...
Source: Iranian Journal of Allergy, Asthma and Immunology - October 14, 2018 Category: Allergy & Immunology Authors: Esmaeili B, Mansouri P, Meysamie A, Izad M Tags: Iran J Allergy Asthma Immunol Source Type: research

Genetic Variation in Intergenic and Exonic miRNA Sequence and Risk of Multiple Sclerosis in the Isfahan Patients.
This study was conducted to investigate the association between mir155 rs767649, mir196a2 rs11614913 and mir23a rs3745453 polymorphism and the risk of multiple sclerosis in the Iranian MS patients in Isfahan. A population of 80 patients and the same number control were selected. After DNA extraction, genotyping was performed through tetra amplification refractory mutation system-PCR method (T ARMS PCR). The frequencies of TT, TC and CC genotypes of mir23a were 46, 35 and 20% in MS patients and 42, 14 and 24 in healthy subjects respectively. These results showed that individuals carrying the genotypes of rs3745453 TC had a ...
Source: Iranian Journal of Allergy, Asthma and Immunology - October 14, 2018 Category: Allergy & Immunology Authors: Golshani Z, Hojati Z, Sharifzadeh A, Shaygannejad V, Jafarinia M Tags: Iran J Allergy Asthma Immunol Source Type: research

Increased Total Serum Immunoglobulin E in Children Developing Mycoplasma pneumoniae-related Extra-pulmonary Diseases.
Abstract Mycoplasma pneumoniae has been recognized to be involved in several extra-pulmonary diseases, but the underlying immunologic mechanisms are still largely unknown. Recently, we observed a significant elevation of serum Immunoglobulin E (IgE) in a small group of these children. Here, we assessed total serum IgE levels in children affected with Mycoplasma pneumoniae-related extra-pulmonary diseases. We prospectively collected the data of 162 children admitted to the hospital (because of respiratory infections or extra-pulmonary diseases) who were evaluated for Mycoplasma pneumoniae serology and total serum I...
Source: Iranian Journal of Allergy, Asthma and Immunology - October 9, 2018 Category: Allergy & Immunology Authors: Poddighe D, Comi EV, Brambilla I, Licari A, Bruni P, Marseglia GL Tags: Iran J Allergy Asthma Immunol Source Type: research

Sensitization and Geographical Distribution of Main Aeroallergens in Iran.
Abstract N0 Abstract. PMID: 30518181 [PubMed - in process] (Source: Iranian Journal of Allergy, Asthma and Immunology)
Source: Iranian Journal of Allergy, Asthma and Immunology - October 7, 2018 Category: Allergy & Immunology Authors: Teifoori F, Postigo I, Abtahi M, Dehghani M, Martinez J Tags: Iran J Allergy Asthma Immunol Source Type: research

Modulation of Macrophage Polarization for Bone Tissue Engineering Applications.
Abstract Innate immune cells play a crucial role in bone development and repair. Macrophages are the main effector cells in immune responses to implants and are indispensable for bone healing success. The heterogeneity and plasticity of macrophages make them a prime target for immune system modulation to enhance bone repair and regeneration. It is believed that the polarization of macrophage phenotype towards the anti-inflammatory M2, rather than the inflammatory M1 phenotype, promotes osteogenesis. Tissue-engineered bioimplants are potentially capable of producing signals to modulate macrophage polarization. Ther...
Source: Iranian Journal of Allergy, Asthma and Immunology - October 7, 2018 Category: Allergy & Immunology Authors: Jamalpoor Z, Asgari A, Lashkari MH, Mirshafiey A, Mohsenzadegan M Tags: Iran J Allergy Asthma Immunol Source Type: research

Modulation of Vascular Endothelial Growth Factor and Annexin A2 in Response to 4-(Methylnitrosamino)-1-(3-pyridyl)-1-Butanone -Induced Inflammation via Swimming Training.
Abstract The nitrosamine 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK; nicotine derived nitrosamine ketone) is one of the strongest carcinogens in tobacco which is involved in induction of lung cancer by changing the stimulation of vascular endothelial growth factor (VEGF) and annexin A2 expression. The aim of this study was to investigate the changes in resting levels of annexin A2 and VEGF in lung tissues of rats exposed NNK after 12 weeks of aerobic submaximal swimming training. For this purpose, 46 Wistar rats were randomly divided into five groups consist of training, training + NNK, NNK, saline and co...
Source: Iranian Journal of Allergy, Asthma and Immunology - October 7, 2018 Category: Allergy & Immunology Authors: Barzegari A, Mirdar S, Ranayi M Tags: Iran J Allergy Asthma Immunol Source Type: research

Neutrophil Extracellular Traps: Formation and Involvement in Disease Progression.
Abstract Neutrophils are the forerunner in innate immunity by defending the host organisms against infectious pathogens. During such process, neutrophils reach the site of inflammation/infection and eliminate the pathogens by phagocytosis as well as by forming the neutrophil extracellular traps (NETs). NETs trap and eradicate a number of microbes including bacteria, fungi, protozoa, viruses. NETs consist of DNA which is decorated with histones and granular proteins such as neutrophil elastase (NE), gelatinase, myeloperoxidase. NETosis (a process of NETs formation) is also involved in many inflammatory and autoimmu...
Source: Iranian Journal of Allergy, Asthma and Immunology - June 1, 2018 Category: Allergy & Immunology Authors: Kumar S, Gupta E, Kaushik S, Jyoti A Tags: Iran J Allergy Asthma Immunol Source Type: research

The Effects of Allium Cepa Extract on Tracheal Responsiveness, Lung Inflammatory Cells and Phospholipase A2 Level in Asthmatic Rats.
In this study, the effects of A. cepa aqueous-alcoholic extract on tracheal responsiveness, lung inflammatory cells and phospholipase A2 (PLA2) level in bronchoalveolar fluid (BALF) of asthmatic rats were examined. Wistar rats were randomly divided into control group (C), asthmatic group (A), asthmatic group (A) treated with A. cepa extract (AC, 0.175, 0.35, and 0.7 mg/mL) and dexamethasone (D, 1.25 μg/mL). The extract of A. cepa and dexamethasone were added to animal's drinking water during sensitization period. Tracheal responsiveness to methacholine and ovalbumin, lung inflammatory cells and PLA2 level in BALF were a...
Source: Iranian Journal of Allergy, Asthma and Immunology - June 1, 2018 Category: Allergy & Immunology Authors: Ghorani V, Marefati N, Shakeri F, Rezaee R, Boskabady M, Boskabady MH Tags: Iran J Allergy Asthma Immunol Source Type: research

Comparison of Fractional Exhaled Nitric Oxide in Elderly Patients with Asthma-chronic Obstructive Pulmonary Disease Overlap and Other Airway Inflammatory Diseases.
This study aimed to investigate the differences in FeNO levels of elderly patients with ACO, asthma, COPD, and chronic cough. We conducted a retrospective study analysing the data of stable outpatients from Pulmonary Department of the Second Clinical College, Jinan University. All participants (Age≥55 years) were divided into the ACO group (n=19), asthma group (n=16), COPD group (n=25), and chronic cough group (n=22). The clinical data such as peripheral eosinophil counts, serum high sensitivity C-reactive protein (hs-CRP), FeNO, and spirometry was collected, and the correlations between FeNO levels and systemic markers...
Source: Iranian Journal of Allergy, Asthma and Immunology - June 1, 2018 Category: Allergy & Immunology Authors: Shi F, Qiu C, Yu J, Yang Y, Li B, Feng M, Zhang T, Liu W Tags: Iran J Allergy Asthma Immunol Source Type: research