Clinical Applications of Interleukin-37: A Key Player in the Immunopathogenesis of Immune Disorders.
Abstract Recently, the era of medicine has been encountered with the exponential growth of special seroimmunobiomarkers in clinical trials. Lately, Interleukin-37 (IL-37) has attracted a wide range of basic medical scientists' attention due to its controversial functions in physiologic or pathologic microenvironments. In this research, an updated overview of immunobiological functions and clinical applications of IL-37 in a wide range of diseases, are discussed in order to highlight the role of recent laboratory-based results of IL-37. Data of this systematic review article were collected from initial 237 articles...
Source: Iranian Journal of Allergy, Asthma and Immunology - June 23, 2020 Category: Allergy & Immunology Authors: Khosh E, Bahmaie N, Elahi R, Esmaeilzadeh A Tags: Iran J Allergy Asthma Immunol Source Type: research

Impact of OCT4 and Its Related Signaling Pathways on Gastrointestinal Cancers: Focusing on Targeted Therapy.
Abstract There are many pieces of evidence support the effect of cancer stem cells on the initiation and progression of cancer. However, related mechanisms involved in these phenomena are far more complicated to understand. The function of different stemness factorsin cancer stem cells (CSCs) and their complex associations at different levels of cancer have been reported. Therefore, it seems that focusing on one master factor would be more helpful to complete the puzzle of singling pathways in these cells. Octamer-binding transcription factor 4 (OCT4) also known as POU domain, class 5, transcription factor 1(POU5F...
Source: Iranian Journal of Allergy, Asthma and Immunology - June 23, 2020 Category: Allergy & Immunology Authors: Cheshomi H, Gholami O, Peyroshabani B, Rad A Tags: Iran J Allergy Asthma Immunol Source Type: research

Effect of Dextrose Prolotherapy, Platelet Rich Plasma and Autologous Conditioned Serum on Knee Osteoarthritis: A Randomized Clinical Trial.
In this study, the clinical efficiency of Dextrose prolotherapy, platelet-rich plasma (PRP) and autologous conditioned serum (ACS) injection on the level of pain and function in Knee OA were compared. A randomized clinical trial was directed on 92 knee OA patients. Patients were randomly divided into three groups: 30 were received dextrose prolotherapy once in a week for three weeks, 30 received autologous PRP for two times with seven days interval, and in the remaining 32 patients 2ml of ACS were injected two times every seven days. Study participants were measured through the Western Ontario and McMaster Universities (WO...
Source: Iranian Journal of Allergy, Asthma and Immunology - June 23, 2020 Category: Allergy & Immunology Authors: Pishgahi A, Abolhasan R, Shakouri SK, Soltani-Zangbar MS, Dareshiri S, Ranjbar Kiyakalayeh S, Khoeilar A, Zamani M, Motavalli Khiavi F, Pourabbas Kheiraddin B, Mehdizadeh A, Yousefi M Tags: Iran J Allergy Asthma Immunol Source Type: research

Immunoregulatory Effects of Krocina ™, a Herbal Medicine Made of Crocin, on Osteoarthritis Patients: A Successful Clinical Trial in Iran.
Immunoregulatory Effects of Krocina™, a Herbal Medicine Made of Crocin, on Osteoarthritis Patients: A Successful Clinical Trial in Iran. Iran J Allergy Asthma Immunol. 2020 Jun 23;19(3):253-263 Authors: Poursamimi J, Shariati-Sarabi Z, Tavakkol-Afshari J, Mohajeri SA, Ghoryani M, Mohammadi M Abstract Osteoarthritis (OA) is the major cause of joint pain and disability. This research was planned to examine the effects of Krocina™, aherbal medicine made of crocin, an ingredient of saffron, in patients with OA. Forty patients suffering from OA were enrolled in our study and randomly divided in...
Source: Iranian Journal of Allergy, Asthma and Immunology - June 23, 2020 Category: Allergy & Immunology Authors: Poursamimi J, Shariati-Sarabi Z, Tavakkol-Afshari J, Mohajeri SA, Ghoryani M, Mohammadi M Tags: Iran J Allergy Asthma Immunol Source Type: research

Construction and Functional Characterization of a Fully Human Anti-mesothelin Chimeric Antigen Receptor (CAR) Expressing T Cell.
Abstract Chimeric antigen receptor (CAR) T cell therapy is considered as an encouraging approach for the treatment of hematological malignancies. However, its efficacy in solid tumors has not been satisfying, mainly in the immunosuppressive network of the tumor microenvironment and paucity of appropriate target antigens. Mesothelin (MSLN) is a tumor-associated antigen (TAA) expressed in numerous types of solid tumors such as gastrointestinal, ovarian, and pancreatic tumors. Owing to high expression in tumor cells and low expression in normal tissues, MSLN-targeted therapies like monoclonal antibodies have been pre...
Source: Iranian Journal of Allergy, Asthma and Immunology - June 23, 2020 Category: Allergy & Immunology Authors: Jafarzadeh L, Masoumi E, Alishah K, Mirzaei HR, Jamali A, Fallah-Mehrjardi K, Rostamian H, Khakpoor-Koosheh M, Meshkani R, Noorbakhsh F, Hadjati J Tags: Iran J Allergy Asthma Immunol Source Type: research

Rational Design of Hypoallergenic Vaccines: Blocking IgE-binding to Polcalcin Using Allergen-specific IgG Antibodies.
Abstract Chenopodium album polcalcin (Che a 3) is characterized as a major cause of cross-reactivity inallergic patients to the Chenopodiaceae family. Therefore, the present study was conducted to develop a hypoallergenic Che a 3 derivatives as the candidate vaccine for type 1 allergy. Four derivatives were generated from Che a 3. The first was a mosaic peptide derivative computationally identified in Che a 3 which was coupled to keyhole limpet hemocyanin (KLH). The second one was a mutant Che a 3, and the other two derivatives included N- and C-terminal halves of Che a 3 that both coupled to KLH. The IgE-binding ...
Source: Iranian Journal of Allergy, Asthma and Immunology - June 23, 2020 Category: Allergy & Immunology Authors: Mohammadi M, Khamisipour G, Soltanpour F, Omrani F, Taheri B, Momenzadeh N, Fouladvand M Tags: Iran J Allergy Asthma Immunol Source Type: research

Increased miR-223-3p in Leukocytes Positively Correlated with IL-17A in Plasma of Asthmatic Patients.
Abstract Asthma is a common airway inflammation with an intricate underlying mechanism. The role played by circulating miRNAs in asthma remains unclear. In the present study, we aimed to investigate the role of miR-223-3p in leukocytes of asthma and identify the relationship between miR-223-3p and inflammatory cytokines in asthma. Using real-time polymerase chain reaction (RT-PCR), we detected miR-223-3p expression in peripheral blood leukocytes from 23 asthmatic patients and 20 healthy controls. The levels of IFN-γ (Th1 cytokine), IL-4 (Th2 cytokine), IL-17A (Th17 cytokine) in plasma were examined using enz...
Source: Iranian Journal of Allergy, Asthma and Immunology - June 23, 2020 Category: Allergy & Immunology Authors: Xu W, Wang Y, Wang C, Ma Y, He S, Kang Y, Yang J Tags: Iran J Allergy Asthma Immunol Source Type: research

A Randomized, Triple-blind Placebo-controlled Trial to Determine the Effect of Saffron on the Serum Levels of MMP-9 and TIMP-1 in Patients with Multiple Sclerosis.
This study aimed to determine the appropriate treatment option for multiple sclerosis (MS). Forty-three relapsing-remitting MS (RRMS) patients were randomly divided into two groups of 22 (group A, placebo) and 21 (group B, Saffron pill) individuals. Serum samples were collected from patients' blood before using the Saffron pills/placebo pills and then after 12 months. The serum level of MMP-9 and its inhibitor, as well as TIMP-1, were measured by ELISA kits. MMP-9 serum levels noticeably decreased in patients with MS following 12 months of treatment with Saffron pills (p=0.006) while the changes were not significant before...
Source: Iranian Journal of Allergy, Asthma and Immunology - June 23, 2020 Category: Allergy & Immunology Authors: Ghasemi Sakha F, Azimi Saeen A, Moazzeni SM, Etesam F, Vaezi G Tags: Iran J Allergy Asthma Immunol Source Type: research

Clinical and Genetic Study of X-linked Agammaglobulinemia Patients (The Benefit of Early Diagnosis).
This study aims to report the clinical and genetic features of five Iranian patients with XLA. Five male cases with recurrent bacterial infection entered this study based on clinical evaluation and Immunological screening tests. The levels of T-cell receptor excision circle (TREC) and kappa-deleting recombination excision circle (KREC) were also measured in dried blood spot (DBS) samples. Sanger sequencing was applied to PCR products of DNA samples of the patients for genetic studies. All patients were from unrelated families with a mean age of 6.7 years (2.5-11) at the time of diagnosis with 4.8 mean years of delay in dia...
Source: Iranian Journal of Allergy, Asthma and Immunology - June 23, 2020 Category: Allergy & Immunology Authors: Alizadeh Z, Dashti P, Mazinani M, Nourizadeh M, Shakerian L, Tajik S, Movahedi M, Mamishi S, Pourpak Z, Fazlollahi MR Tags: Iran J Allergy Asthma Immunol Source Type: research

A Rare Case with Quail Egg Allergy without Allergic Reactions to Oral Food Challenge with Hen's Egg White.
Abstract Hen's egg, as one of the most common reasons for IgE-mediated food hypersensitivity, affects both children and adults. Taking precautionary measures is suggested for the consumption of other birds' eggs for patients with allergy to hen's egg. This paper describes a rare patient with quail egg allergy, which manifested no allergic reactions after oral food challenge with hen's egg white. PMID: 32615665 [PubMed - in process] (Source: Iranian Journal of Allergy, Asthma and Immunology)
Source: Iranian Journal of Allergy, Asthma and Immunology - June 23, 2020 Category: Allergy & Immunology Authors: Ghobadi Dana V, Bemanian MH, Shokouhi Shoormasti R, Arshi S, Nabavi M Tags: Iran J Allergy Asthma Immunol Source Type: research

Delay in Diagnosis of Two Siblings with Severe Ocular Problems and Autoimmune Polyglandular Syndrome.
Abstract Autoimmune polyendocrine syndrome type 1 (APS1) is a scarce polyendocrinopathy with autosomal recessive inheritance results from defects in the human autoimmune regulatory (AIRE) gene. In addition to three major manifestations of APS1 including mucocutaneous candidiasis, hypoparathyroidism, and Addison's disease, ophthalmic problems such as keratoconjunctivitis, dry eye, iridocyclitis, and cataract can be seen in these patients. In this article, we introduced two siblings presented with nail dystrophia, severe photophobia, and keratitis since early childhood which genetic examination revealed single nucle...
Source: Iranian Journal of Allergy, Asthma and Immunology - June 23, 2020 Category: Allergy & Immunology Authors: Sharafian S, Tavakol M, Gharagozlou M, Parvaneh N Tags: Iran J Allergy Asthma Immunol Source Type: research

Response Comment on "Association between Interleukin-32 and Interleukin-17A Single Nucleotide Polymorphisms and Serum Levels with Polycystic Ovary Syndrome".
Response Comment on "Association between Interleukin-32 and Interleukin-17A Single Nucleotide Polymorphisms and Serum Levels with Polycystic Ovary Syndrome". Iran J Allergy Asthma Immunol. 2020 Jun 23;19(3):320-322 Authors: Gharesi-Fard B, Hesampour F Abstract No abstract! PMID: 32615668 [PubMed - in process] (Source: Iranian Journal of Allergy, Asthma and Immunology)
Source: Iranian Journal of Allergy, Asthma and Immunology - June 23, 2020 Category: Allergy & Immunology Authors: Gharesi-Fard B, Hesampour F Tags: Iran J Allergy Asthma Immunol Source Type: research

COVID-19 Pandemic: A Big Challenge in Iran and the World.
Abstract No abstract! PMID: 32534504 [PubMed - as supplied by publisher] (Source: Iranian Journal of Allergy, Asthma and Immunology)
Source: Iranian Journal of Allergy, Asthma and Immunology - May 17, 2020 Category: Allergy & Immunology Authors: Nourizadeh M, Rasaee MJ, Moin M Tags: Iran J Allergy Asthma Immunol Source Type: research

Sitagliptin Repositioning in SARS-CoV-2: Effects on ACE-2, CD-26, and Inflammatory Cytokine Storms in the Lung.
Abstract No Abstract. PMID: 32534505 [PubMed - as supplied by publisher] (Source: Iranian Journal of Allergy, Asthma and Immunology)
Source: Iranian Journal of Allergy, Asthma and Immunology - May 17, 2020 Category: Allergy & Immunology Authors: Dastan F, Abedini A, Shahabi S, Kiani A, Saffaei A, Zare A Tags: Iran J Allergy Asthma Immunol Source Type: research

Bradykinin as a Probable Aspect in SARS-Cov-2 Scenarios: Is Bradykinin Sneaking out of Our Sight?
Abstract The new virus SARS-CoV-2 is savagely spreading out over the world. The biologic studies show that the target receptor for the virus might be angiotensin-converting enzyme 2 (ACE2). This peptide is responsible for converting angiotensin II (Ang II), which is a profoundly active peptide, into Ang 1-7 with quite a balancing barbell function. It is emphasized that the direct target of the virus is ACE2 underlining the obvious difference with ACE. Nevertheless, we hypothesized that a back load build up effect on Ang II may usurp the ACE capacity and subsequently leave the bradykinin system unabated. We think t...
Source: Iranian Journal of Allergy, Asthma and Immunology - May 17, 2020 Category: Allergy & Immunology Authors: Ghahestani SM, Mahmoudi J, Hajebrahimi S, Sioofy-Khojine AB, Salehi-Pourmehr H, Sadeghi-Ghyassi F, Mostafaei H Tags: Iran J Allergy Asthma Immunol Source Type: research

Forty Years of Research and Treatment in Immunology and Allergy: In Honor of Professor Reza Farid Hosseini.
Abstract In light of various supports of prodigious figures in the field of immunology and allergy, the subject area has been faced a great leap during the last century. The current state of the discipline owes an abundant appreciation for the scholars motivated in escalating the true nature of the science, who left no stone unturned in improving the general common sense and understanding of the human knowledge in general, and immunology and allergy in particular. Professor Reza Farid Hosseini is among the dignitaries who invested his life and energy on weaving the tapestry of the immunology and allergy. He delive...
Source: Iranian Journal of Allergy, Asthma and Immunology - May 17, 2020 Category: Allergy & Immunology Authors: Jafari R, Moin M, Kashanchi F, Rajbar A Tags: Iran J Allergy Asthma Immunol Source Type: research

Crocus Sativus (Saffron): An Immunoregulatory Factor in the Autoimmune and Non-autoimmune Diseases.
Abstract It has been reported that patients with arthritis, osteoarthritis, atherosclerosis, coronary artery disease, brain ischemia, diabetes, and inflammatory bowel disease (IBD) suffer from pro-inflammatory and oxidant related responses. Therefore, anti-inflammatory and anti-oxidant therapies are used to improve the quality of life of the patients. Saffron is a herbal drug that has immunomodulatory and antioxidant properties. Hence, Saffron and its components have been proposed as therapeutic agents for the treatment of the diseases. Therefore, this review article was designed to collect recent information rega...
Source: Iranian Journal of Allergy, Asthma and Immunology - May 17, 2020 Category: Allergy & Immunology Authors: Poursamimi J, Shariati-Sarabi Z, Tavakkol-Afshari J, Mohajeri SA, Mohammadi M Tags: Iran J Allergy Asthma Immunol Source Type: research

Management of COVID-19 Virus Infection by Convalescent Plasma.
Abstract No Abstract. PMID: 32534509 [PubMed - as supplied by publisher] (Source: Iranian Journal of Allergy, Asthma and Immunology)
Source: Iranian Journal of Allergy, Asthma and Immunology - May 17, 2020 Category: Allergy & Immunology Authors: Cheraghali AM, Abolghasemi H, Eshghi P Tags: Iran J Allergy Asthma Immunol Source Type: research

In Vitro Effects of Sodium Benzoate on the Expression of T Cells-related Cytokines and Transcription Factors in Adjuvant-induced Arthritis Model.
In conclusion, NaB can potentially be considered as a useful therapeutic agent for the treatment of RA and other Th1 and Th17-mediated diseases. PMID: 32534510 [PubMed - as supplied by publisher] (Source: Iranian Journal of Allergy, Asthma and Immunology)
Source: Iranian Journal of Allergy, Asthma and Immunology - May 17, 2020 Category: Allergy & Immunology Authors: Bemani P, Amirghofran Z, Kamali-Sarvestani E Tags: Iran J Allergy Asthma Immunol Source Type: research

Curcumin Ameliorate Diabetes type 1 Complications through Decreasing Pro-inflammatory Cytokines in C57BL/6 Mice.
Abstract Type 1 diabetes is a chronic autoimmune disease of beta cells in the islets of Langerhans, which are responsible for making insulin. Even with insulin therapy, inflammatory complications will develop in the long term. The present study examines changes in serum levels of interleukin (IL)-6, IL-17, IL-10, tumor necrosis factor (TNF)-α, interferon (IFN)-γ, C-peptide, Insulin as well as fasting blood sugar (FBS) in control, diabetic and diabetic treated with curcumin groups. Thirty inbred C57BL /6 mice were randomly divided into three groups of 10 mice: group A consisted of healthy mice receiving...
Source: Iranian Journal of Allergy, Asthma and Immunology - May 17, 2020 Category: Allergy & Immunology Authors: Jafari Khataylou Y, Ahmadiafshar S, Rezaei R, Parsamanesh S, Hosseini G Tags: Iran J Allergy Asthma Immunol Source Type: research

Anti-inflammatory Effects of Matricaria chamomilla Extracts on BALB/c Mice Macrophages and Lymphocytes.
In this study, we aimed to report the amount of this compound by liquid chromatography-mass spectrometry (LC-MS) and measuring the total phenol content (TPC) in both the MC aqueous and alcoholic extracts. We also investigated the MC aqueous and ethanolic extracts effect on BALB/c separated macrophages and lymphocytes cell viability and macrophage nitric oxide production. Interferon-γ and interleukin-10 secretion were also measured in lymphocytes. We found that the amount of apigenin was 0.078 and 0.25 mg/g per each of dry aqueous and alcoholic extracts, respectively. Also, the total phenol content was 2.99% in aqueou...
Source: Iranian Journal of Allergy, Asthma and Immunology - May 17, 2020 Category: Allergy & Immunology Authors: Asadi Z, Ghazanfari T, Hatami H Tags: Iran J Allergy Asthma Immunol Source Type: research

COVID-19 Pandemic Hemoperfusion Therapy Versus Plasma Exchange Therapy in Intensive Care.
Abstract No abstract. PMID: 32534513 [PubMed - as supplied by publisher] (Source: Iranian Journal of Allergy, Asthma and Immunology)
Source: Iranian Journal of Allergy, Asthma and Immunology - May 17, 2020 Category: Allergy & Immunology Authors: Esmaeili Vardanjani A, Moayedi S, Golitaleb M Tags: Iran J Allergy Asthma Immunol Source Type: research

Pro-inflammatory Effects of Influenza Type A Virus PB1-F2 Protein-derived Peptide in Lipopolysaccharide-treated Macrophages.
Abstract Influenza A virus (IAV) has the potential to cause pandemics with considerable health and socio-economic burdens. A viral protein, polymerase basic 1- frame2 (PB1-F2), as a virulence factor, has pro-apoptotic activity and contributes to viral pathogenesis by delaying viral clearance and inducing inflammation. Macrophages are susceptible to IAV infection and produce high levels of inflammatory cytokines and chemokines. In the present study, the pro-inflammatory effects of PB1-F2 derived peptide was evaluated by measuring the expression of key inflammatory mediators in murine macrophage cell line J774.1. PB...
Source: Iranian Journal of Allergy, Asthma and Immunology - May 17, 2020 Category: Allergy & Immunology Authors: Kalantar K, Farzaneh Z, Gholijani N, Hosseini SY, Bani Hasan E Tags: Iran J Allergy Asthma Immunol Source Type: research

Evaluation of Inflammatory State in Migraineurs: A Case-control Study.
Abstract Due to inconclusive findings of previous researches, we aimed to evaluate inflammatory state biomarkers in episodic and chronic migraineurs (EM and CM patients) compared to headache-free controls in the current study. Seventy-one migraine patients and 19 age-sex-matched controls were recruited. After obtaining demographic data and recording headache characteristics, blood samples were gathered and analyzed to evaluate the serum levels of C-reactive protein (CRP), tumor necrosis factor(TNF)-α and interleukin (IL)-6. Serum levels of IL-6, CRP and TNF-α were significantly higher among subjects wi...
Source: Iranian Journal of Allergy, Asthma and Immunology - May 17, 2020 Category: Allergy & Immunology Authors: Togha M, Razeghi Jahromi S, Ghorbani Z, Ghaemi A, Rafiee P Tags: Iran J Allergy Asthma Immunol Source Type: research

Multiple Evanescent White Dot Syndrome: A Case Report and Experience with Corticosteroid Therapy.
Abstract Multiple evanescent white dot syndrome (MEWDS) is an inflammatory eye disease of the outer retina, retinal pigmented epithelium, choroid presenting with photopsia, loss of vision, and temporal scotoma. The patient was a 31-year-old female with a history of vision loss since 11 days ago (left eye). At presentation, best-corrected Snellen visual acuity was 20/140 in the Snellen chart. We decided to treat her with short time corticosteroid therapy (0.75 mg/kg/day prednisolone which was tapered in 3 weeks) for any possible rapid recovery of vision. The visual acuity of the involved eye was improved to 20/25 a...
Source: Iranian Journal of Allergy, Asthma and Immunology - May 17, 2020 Category: Allergy & Immunology Authors: Norooznezhad AH, Mohammadzadeh V, Kadivar S, Ghassemi F Tags: Iran J Allergy Asthma Immunol Source Type: research

Prognostic and Clinical Value of CD44 and CD133 in Esophageal Cancer: A Systematic Review and Meta-analysis.
In conclusion, CD44 overexpression was associated with a 5-year overall survival rate and thus this biomarker can be a suitable prognostic tool in esophageal cancer. PMID: 32372624 [PubMed - in process] (Source: Iranian Journal of Allergy, Asthma and Immunology)
Source: Iranian Journal of Allergy, Asthma and Immunology - April 16, 2020 Category: Allergy & Immunology Authors: Kamil Mohammed Al-Mosawi A, Cheshomi H, Hosseinzadeh A, M Matin M Tags: Iran J Allergy Asthma Immunol Source Type: research

Iranian Society of Asthma and Allergy Codes of Professional Ethics.
Abstract The advances in science and technology in recent decades, especially in medical sciences, have raised new ethical challenges. Hence, professional organizations in the field of medical science are trying to develop regulations in the field of medical ethics to help medical science professionals in making the best decisions in different circumstances and moral dilemmas. The organizations also try to monitor their performance using those regulations. On the other hand, due to the specialization of medical science as well as the complexity of communication between these disciplines, there is a growing need fo...
Source: Iranian Journal of Allergy, Asthma and Immunology - April 16, 2020 Category: Allergy & Immunology Authors: Shamsi-Gooshki E, Ahmadi Pishkuhi M, Mousavi MS, Raoofi A, Fazlollahi MR, Parsapour A, Moin M Tags: Iran J Allergy Asthma Immunol Source Type: research

Evaluating the Immunoreactivity of Ailanthus Altissima (The Tree of Heaven) Pollen Extract in Atopic Patients.
Abstract IgE-mediated hypersensitivity reaction to pollens is a common health problem in atopic patients. In this regard, the assessment of the allergenicity of highly pollinating plants would be demanding. Based on the increment of Ailanthus altissima (A. altissima) tree in some parts of Iran and considering its probable role in respiratory allergy, in this study, we aimed to investigate its IgE-immunoreactivity and in diagnostic applications. One hundred and twenty-five allergic rhinitis patients who were diagnosed as high IgE responders and demonstrated seasonal rhinitis or rhinoconjunctivitis, as well as 20 he...
Source: Iranian Journal of Allergy, Asthma and Immunology - April 16, 2020 Category: Allergy & Immunology Authors: Samei A, Fallahpour M, Bolouri MR, Mahmoudi AR, Nasri F, Seif F, Khoshmirsafa M, Batooli H, Shekarabi M, Falak R Tags: Iran J Allergy Asthma Immunol Source Type: research

Production and Characterization of Monoclonal Antibody against Vit v1: A Grape Allergen Belonging to Lipid Transfer Protein Family.
Abstract Allergy to non-specific lipidtransfer protein (nsLTP), the major allergen of grape (Vit v1), is considered as one of the most common fruit allergies in Iran. Therefore, a specific monoclonal antibody (mAb) can be used for the characterization and assessment of. Accordingly, this study aimed to generate and characterize a mAb against Vit v1 with a diagnostic purpose. To this end, Vit v1 allergen (9 kDa) was extracted using a modified Bjorksten extraction method. Natural Vit v1-immunized mouse splenocytes were fused with SP2/0Ag-14 myeloma cells for generating hybridoma cells. Specific antibody-secreting Hy...
Source: Iranian Journal of Allergy, Asthma and Immunology - April 16, 2020 Category: Allergy & Immunology Authors: Hosseinpour M, Mashayekhi K, Falak R, Jamalzehi S, Mohammadian Haftcheshmeh S, Mousavi MJ, Soleimani A, Koushki K, Sankian M, Soukhtanloo M Tags: Iran J Allergy Asthma Immunol Source Type: research

High-level Expression and One-step Purification of Chimeric Antigen Containing HTLV-I-II Diagnostic Epitopes in Escherichia coli.
In this study, our aim was to design a chimeric protein for the simultaneous detection of HTLV-I and HTLV-II antibodies. Immunodominant B cell linear epitopes of envelope and capsid proteins of HTLV-I/-II were selected and linked together; using a suitable amino acid linker and a chimeric antigen (CA). The codon-optimized synthetic DNA encoding the CA was subcloned into the pGS21aexpression vector and CA expressed as His-GST fused protein in E. coli BL21 (DE3) cells. Then the recombinant CA was purified, using the Ni-NTA (Nickle Nitrilotriacetic acid) affinity chromatography under native conditions. The Sodium do...
Source: Iranian Journal of Allergy, Asthma and Immunology - April 16, 2020 Category: Allergy & Immunology Authors: Bezhgi M, Fazilati M Tags: Iran J Allergy Asthma Immunol Source Type: research

Immunogenicity Evaluation of Recombinant Staphylococcus aureus Enterotoxin B (rSEB) and rSEB-loaded Chitosan Nanoparticles Following Nasal Administration.
Abstract Staphylococcal enterotoxin B (SEB), apotent superantigen, is responsible for many disorders caused by Staphylococcus aureus. With regard to the appearance of multidrug-resistant strains of the bacteria, there is a great need to develop an efficient vaccine against this pathogen. In the present study, the immunogenicity of recombinant SEB was evaluated following nasal administration to BABLB/c mice. Indeed, the rSEB protein was entrapped into chitosan nanoparticles and the immunogenicity of nano-formulation was investigated. SEB protein was expressed in E. coli BL21 (DE3) and purified by using a nickel col...
Source: Iranian Journal of Allergy, Asthma and Immunology - April 16, 2020 Category: Allergy & Immunology Authors: Hosseini SA, Nazarian S, Ebrahimi F, Hajizade A Tags: Iran J Allergy Asthma Immunol Source Type: research

Optimized Dose of Dendritic Cell-based Vaccination in Experimental Model of Tumor Using Artificial Neural Network.
In this study, a mathematical model based on an artificial neural network (ANN) was used to predict several patterns and dosage of matured DC administration for improved vaccination. The ANN model predicted that repeated co-injection of tumor antigen (TA)-loaded DCs matured with CpG (CpG-DC) and LML (List-DC) results in improved antitumor immune response as well as a reduction of immunosuppression in the tumor microenvironment. In the present study, we evaluated the ANN prediction accuracy about DC-based cancer vaccines pattern in the treatment of Wehi164 fibrosarcoma cancer-bearing mice. Our results showed that the admini...
Source: Iranian Journal of Allergy, Asthma and Immunology - April 16, 2020 Category: Allergy & Immunology Authors: Mirsanei Z, Habibi S, Kheshtchin N, Mirzaei R, Arab S, Zand B, Jadidi-Niaragh F, Safvati A, Sharif-Paghaleh E, Arabameri A, Asemani D, Hajati J Tags: Iran J Allergy Asthma Immunol Source Type: research

The Effects of Evening Primrose/Hemp Seed Oil Compared to Rapamycin on the Gene Expression of Immunological Parameters in Experimental Autoimmune Encephalomyelitis Splenocytes.
In conclusion, the EP/HS oil is likely to be involved in transcription of factors in favor of EAE improvement as well as participating in remyelination in the EAE spinal cord and that it suggests to be effective in therapeutic approaches for MS. PMID: 32372631 [PubMed - in process] (Source: Iranian Journal of Allergy, Asthma and Immunology)
Source: Iranian Journal of Allergy, Asthma and Immunology - April 16, 2020 Category: Allergy & Immunology Authors: Rezapour-Firouzi S, Mohammadian M, Sadeghzadeh M, Mehranfar S, Mazloomi E Tags: Iran J Allergy Asthma Immunol Source Type: research

Exome-first Approach Identified Novel Homozygous Dedicator of Cytokinesis 8 (DOCK8) Mutations in Three Unrelated Iranian Pedigrees Suspected with Hyper-IgE Syndrome.
Abstract The prevalence of primary immunodeficiency (PID) is rather high in Iran compared to the world average, mainly due to the high rate of consanguineous marriage. Despite that, little genetic information is available about primary immunodeficiencies in Iran. Autosomal recessive hyper IgE syndrome (AR-HIES) is a severe type of immunodeficiency, mainly caused by mutations in the dedicator of cytokinesis 8 (DOCK8). Rapid and precise diagnoses of patients suffering from AR-HIES can help to manage the patients and reach properly the treatment decision. However, in regions with low financial resources and limited e...
Source: Iranian Journal of Allergy, Asthma and Immunology - April 16, 2020 Category: Allergy & Immunology Authors: Aghebati-Maleki A, Shahani T, Momen T, Alyasin S, Changi-Ashtiani M, Biglari A, Shahrooei M, Javanian AS, Amini S, Bossuyt X, Rokni-Zadeh H Tags: Iran J Allergy Asthma Immunol Source Type: research

Anaphylaxis to Oatmeal and Psocid Crisps.
Abstract Occasionally, a seemingly straightforward history of food-induced anaphylaxis may prove to be misleading.  Both patients and their physicians have a tendency to attribute the cause of an allergic reaction to the most conspicuous ingredient that had been ingested while overlooking less likely causes.  Here, we describe a patient whose history pointed to oatmeal allergy, but skin prick tests to oats and serologic testing for oat-specific IgE were negative. Ultimately, we found that the oatmeal had been contaminated with an allergenic insect, Psocid of the order Psocoptera. PMID: 32372633 [Pub...
Source: Iranian Journal of Allergy, Asthma and Immunology - April 16, 2020 Category: Allergy & Immunology Authors: Babaei D, Vadas P Tags: Iran J Allergy Asthma Immunol Source Type: research

The CD45dim/CD123bright/HLADRneg BAT in the Anti-histamine Drug Allergy.
Abstract No Abstract. PMID: 32372634 [PubMed - in process] (Source: Iranian Journal of Allergy, Asthma and Immunology)
Source: Iranian Journal of Allergy, Asthma and Immunology - April 16, 2020 Category: Allergy & Immunology Authors: Chirumbolo S, Bjorklund G Tags: Iran J Allergy Asthma Immunol Source Type: research

Autologous Whole-blood Injections in Chronic Spontaneous Urticaria: Assessment of Efficacy Biomarkers.
Abstract No abstract. PMID: 32372635 [PubMed - in process] (Source: Iranian Journal of Allergy, Asthma and Immunology)
Source: Iranian Journal of Allergy, Asthma and Immunology - April 16, 2020 Category: Allergy & Immunology Authors: Kitsioulis NA, Zafiriou E, Xepapadaki P, Taka S, Papadopoulos NG, Roussaki-Schulze AV Tags: Iran J Allergy Asthma Immunol Source Type: research

Serum Levels of Interleukin 18 and Interleukin 10 in Iranian Patients with Bullous Pemphigoid and Their Correlation with BP180-NC16a and BP230.
Abstract No Abstract. PMID: 32245316 [PubMed - as supplied by publisher] (Source: Iranian Journal of Allergy, Asthma and Immunology)
Source: Iranian Journal of Allergy, Asthma and Immunology - February 1, 2020 Category: Allergy & Immunology Authors: Esmaili N, Mortazavi H, Ansari MS, Nasimi M, Ardalanrad Y, Teimourpour A Tags: Iran J Allergy Asthma Immunol Source Type: research

Evaluation of Serum Substance P Level in Chronic Urticaria and Correlation with Disease Severity.
In conclusion, Using SP antagonist drugs could be a potential treatment for chronic urticaria. PMID: 32245317 [PubMed - as supplied by publisher] (Source: Iranian Journal of Allergy, Asthma and Immunology)
Source: Iranian Journal of Allergy, Asthma and Immunology - February 1, 2020 Category: Allergy & Immunology Authors: Fadaee J, Khoshkhui M, Emadzadeh M, Hashemy SI, Farid Hosseini R, Jabbari Azad F, Ahanchian H, Lavi Arab F Tags: Iran J Allergy Asthma Immunol Source Type: research

Comparison of Diagnostic Tests with Oral Food Challenge in a Clinical Trial for Adult Patients with Sesame Anaphylaxis.
In conclusion, when the OFC is not possible, the SPP test with natural sesame seed may be applicable in patients with a convincing history instead of the artificial or commercial extracts of sesame used for SPT. Positive SPP is a good alternative diagnostic method for patients with sesame anaphylaxis. Also, the poor sensitivity of SPT and sIgE may indicate the poor discriminative capability of these tests. PMID: 32245318 [PubMed - as supplied by publisher] (Source: Iranian Journal of Allergy, Asthma and Immunology)
Source: Iranian Journal of Allergy, Asthma and Immunology - February 1, 2020 Category: Allergy & Immunology Authors: Salari F, Bemanian MH, Fallahpour M, Tavakol M, Shokri S, Baniadam L, Khoshmirsafa M, Seif F, Nabavi M, Arshi S Tags: Iran J Allergy Asthma Immunol Source Type: research

Activation of Invariant Natural Killer T Cell Subsets in C57BL/6J Mice by Different Injection Modes of α-galactosylceramide.
This study included healthy control, subcutaneous injection, and intraperitoneal injection groups (n=10 in each group). The subcutaneous and intraperitoneal injection groups were injected with α-Galcer (0.1 mg/kg weight), and then the changes in thymus, spleen, and liver iNKT cell frequencies and subsets were observed. The intraperitoneal injection of α-GalCer could increase the frequency of splenic iNKT cells, but the subcutaneous injection did not affect the frequency. Neither injection had any effect on the frequency of iNKT cells in the thymus and liver. The subcutaneous injection of α-GalCer increase...
Source: Iranian Journal of Allergy, Asthma and Immunology - February 1, 2020 Category: Allergy & Immunology Authors: Meng M, Chen S, Gao X, Liu H, Zhao H, Zhang J, Zhang J, Chen D Tags: Iran J Allergy Asthma Immunol Source Type: research

Effects of Cigarette Smoke and Opium on the Expression of CD9, CD36, and CD68 at mRNA and Protein Levels in Human Macrophage Cell Line THP-1.
Abstract Cigarette smoking and opium use are risk factors for coronary artery disease (CAD). It has been known that scavenger receptors such as CD36 and CD68 play critical roles in the pathogenesis of CAD. CD9, as a member of the tetraspanin, has been shown to interact with scavenger receptors. The aim of this study was to investigate the effects of these risk factors on expression levels of CD9, CD36, and CD68 on the THP-1 cell line. The THP-1 cell line treated with cigarette smoke extract (CSE( and opium, both individually and combinatory, in 24 h incubation. The protein and mRNA levels of CD9, CD36, and CD68 we...
Source: Iranian Journal of Allergy, Asthma and Immunology - February 1, 2020 Category: Allergy & Immunology Authors: Momeni-Moghaddam MA, Asadikaram G, Nematollahi MH, Esmaeili Tarzi M, Faramarz-Gaznagh S, Mohammadpour-Gharehbagh A, Kazemi Arababadi M Tags: Iran J Allergy Asthma Immunol Source Type: research

The Association between Inflammatory Cytokines and miRNAs with Slow Coronary Flow Phenomenon.
This study provided the potential role of miRNAs as biomarkers for SCF diagnosis as well as suitable markers for monitoring coronary artery disease (CAD) development in these patients. More investigations are still necessary to unravel the detailed essential mechanisms of circulating miRNA levels in patients with heart failure and SCF. PMID: 32245321 [PubMed - as supplied by publisher] (Source: Iranian Journal of Allergy, Asthma and Immunology)
Source: Iranian Journal of Allergy, Asthma and Immunology - February 1, 2020 Category: Allergy & Immunology Authors: Danaii S, Shiri S, Dolati S, Ahmadi M, Ghahremani-Nasab L, Amiri A, Kamrani A, Samadi Kafil H, Chakari-Khiavi F, Hojjat-Farsangi M, Malek Mahdavi A, Mehdizadeh A, Yousefi M Tags: Iran J Allergy Asthma Immunol Source Type: research

Comparing the Frequency of CD4+T Cells in Recurrent Spontaneous Abortion Women with and without Anti-thyroid Peroxidase (TPO).
In this study, peripheral blood samples were obtained from three groups of women (age: 20-35 years) including RSA anti-TPO positive (n=17), RSA anti-TPO negative (n=27), and fertile (n=29) groups. The frequency of T helper (Th) 1, Th2, Th17, and regulatory T cells (Tregs) and also, the proportions of Th1/Th2 and Th17/Treg were measured by flow cytometry and compared between groups in different menstrual phases. The findings indicated elevated levels of Th1 in anti-TPO+ RSA in comparison with those without anti-TPO (p-value: 0.004), exclusively in the luteal phase. Other T cell subsets were different only between RSA and co...
Source: Iranian Journal of Allergy, Asthma and Immunology - February 1, 2020 Category: Allergy & Immunology Authors: Basimi P, Akbari Asbagh F, Yekaninejad MS, Asadi M, Dabbagh A, Najafi Arbastan F, Rajaei S Tags: Iran J Allergy Asthma Immunol Source Type: research

Assessment of miR-181b-5p, miR-23a-3p, BCL-2, and IL-6 in Peripheral Blood Mononuclear Cells of Autistic Patients; Likelihood of Reliable Biomarkers.
Abstract Autism is a neurodevelopmental disorder that is recognized by stereotypic and repetitive behaviors after 2 years of old. Dysregulation of the immune system, especially inflammation which is mostly regulated by IL-6, imposes a deficit in CNS development. Along with this crucial biomarker, researchers have proposed BCL-2, micro RNA-23a-3p (miR-23a-3p), miR-181b-5p as other probable biomarkers involved in inflammation and apoptosis. The aim of the study was to evaluate the alteration in the expression of these biomarkers in a group of autism spectrum disorder (ASD) children. Peripheral blood mononuclear cell...
Source: Iranian Journal of Allergy, Asthma and Immunology - February 1, 2020 Category: Allergy & Immunology Authors: Atwan H, Assarehzadegan MA, Shekarabi M, Jazayeri SM, Barfi S, Shokouhi Shoormasti R, Chimeh N, Pouretemad HR, Tayebi B Tags: Iran J Allergy Asthma Immunol Source Type: research

In Vitro Effects of Curcumin on Transforming Growth Factor- β-mediated Non-Smad Signaling Pathway, Oxidative Stress, and Pro-inflammatory Cytokines Production with Human Vascular Smooth Muscle Cells.
In Vitro Effects of Curcumin on Transforming Growth Factor-β-mediated Non-Smad Signaling Pathway, Oxidative Stress, and Pro-inflammatory Cytokines Production with Human Vascular Smooth Muscle Cells. Iran J Allergy Asthma Immunol. 2020 Feb 01;19(1):84-93 Authors: Asadi A, Yaghobi Nezhad D, Rafie Javazm A, Khanicheragh P, Mashouri L, Shakeri F, Abbasi M, Afrazian MS, Niknam Z, Abazari O Abstract Transforming growth factor-β (TGF-β) induces pro-inflammatory cytokines expression including interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α) and these cytokines are associated w...
Source: Iranian Journal of Allergy, Asthma and Immunology - February 1, 2020 Category: Allergy & Immunology Authors: Asadi A, Yaghobi Nezhad D, Rafie Javazm A, Khanicheragh P, Mashouri L, Shakeri F, Abbasi M, Afrazian MS, Niknam Z, Abazari O Tags: Iran J Allergy Asthma Immunol Source Type: research

Inflammatory and T Helper 17/ Regulatory T Cells Related Cytokines Balance in Cutaneous Lupus Erythematosus (CLE).
In conclusion, inflammatory cytokines such as IL-6 involved in inflammation and differentiation of Th17 cells are probably responsible in part for Th17 activity in CLE. IL-17, IL-23, and IL-6/IL-6R (IL-6 receptor) inhibitors may be good treatments for CLE patients. So targeting these cytokines activity pathways can improve the CLE treatment strategy and may open a novel guideline for SLE and CLE treatment. PMID: 32245325 [PubMed - as supplied by publisher] (Source: Iranian Journal of Allergy, Asthma and Immunology)
Source: Iranian Journal of Allergy, Asthma and Immunology - February 1, 2020 Category: Allergy & Immunology Authors: Yazdani MR, Aflaki E, Gholijani N Tags: Iran J Allergy Asthma Immunol Source Type: research

A Novel Non-frameshift ADA Deletion Detected by Whole Exome Sequencing in an Iranian Family with Severe Combined Immunodeficiency.
In conclusion, we here describe a novel mutation in ADA, a well-known SCID gene. PMID: 32245326 [PubMed - as supplied by publisher] (Source: Iranian Journal of Allergy, Asthma and Immunology)
Source: Iranian Journal of Allergy, Asthma and Immunology - February 1, 2020 Category: Allergy & Immunology Authors: Talebi T, Biglari A, Shahroeei M, Changi-Ashtiani M, Dinmohammadi H, Navabi SS, Parvaneh N, Bossuyt X, Shahani T, Rokni-Zadeh H Tags: Iran J Allergy Asthma Immunol Source Type: research

Hsp70 in Cancer: Partner or Traitor to Immune System.
Abstract Heat shock protein 70.1 (Hsp70.1), also known as Hsp70, is a highly conserved member of the heat shock protein family that exists in all living organisms and determines the protein fate as molecular chaperones. Hsp70 basal expression is undetectable or low in most unstressed normal cells, however, its abundant presence in several types of human cancer cells is reported. Several studies support upregulated Hsp70 involved in tumor progression and drug resistance through modulation of cell death pathways and suppresses anticancer immune responses. However, numerous studies have confirmed that Hsp70 can also ...
Source: Iranian Journal of Allergy, Asthma and Immunology - December 4, 2019 Category: Allergy & Immunology Authors: Vostakolaei MA, Abdolalizadeh J, Hejazi MS, Kordi S, Molavi O Tags: Iran J Allergy Asthma Immunol Source Type: research

Analysis of Methylation and Expression Profile of Foxp3 Gene in Patients with Beh çet's Syndrome.
This study was performed to assess the role of the foxp3 gene in patients with Behçet's syndrome (BS). Venous blood samples were collected from all participants and peripheral blood mononuclear cells (PBMC) were extracted through Ficoll-Hypaque method. Genomic DNA was randomly sheared by sonication and immunoprecipitated with a monoclonal antibody. The status methylation of the foxp3 gene was estimated in 108 blood samples of active BS patients and healthy individuals (controls); using methylation DNA immunoprecipitation (MeDIP) technique. Expression analysis was carried out; using Real-time PCR. The expression of f...
Source: Iranian Journal of Allergy, Asthma and Immunology - December 3, 2019 Category: Allergy & Immunology Authors: Farhadi J, Nouri M, Khabbazi A, Samadi N, Babaloo Z, Azad M, Abolhasani S, Alipour S, Jadideslam G, Seydi Shirvani S, Sakhinia E Tags: Iran J Allergy Asthma Immunol Source Type: research