TACC3 expression as a prognostic factor in aggressive types of adult T-cell leukemia/lymphoma patients.
We examined tissue microarrays of histological samples from 92 cases of aggressive ATLL and assessed clinical features, including TACC3 protein expression levels. RESULTS: Compared with TACC3-low, TACC3-high ATLL patients were significantly older (P 
Source: International Journal of Laboratory Hematology - August 3, 2020 Category: Hematology Authors: Moritsubo M, Miyoshi H, Matsuda K, Yoshida N, Nakashima K, Yanagida E, Yamada K, Takeuchi M, Suzuki T, Muta H, Umeno T, Furuta T, Seto M, Ohshima K Tags: Int J Lab Hematol Source Type: research

Mutation profile and prognostic relevance in elderly patients with de novo acute myeloid leukemia treated with decitabine-based chemotherapy.
CONCLUSION: This study comprehensively analyzed the prognostic implications of gene mutations in elderly AML patients under decitabine-based treatment modality. Identification of genetic biomarkers to predict the subgroup of elderly AML patients who can benefit from decitabine-based regimens might have an immediate clinical utility to optimize the treatment of elderly AML patients. PMID: 32730663 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology)
Source: International Journal of Laboratory Hematology - July 30, 2020 Category: Hematology Authors: Ni J, Hong J, Long Z, Li Q, Xia R, Zeng Q Tags: Int J Lab Hematol Source Type: research

Peripheral blood morphologic findings in patients with COVID-19.
PMID: 32730694 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology)
Source: International Journal of Laboratory Hematology - July 30, 2020 Category: Hematology Authors: Sadigh S, Massoth LR, Christensen BB, Stefely JA, Keefe J, Sohani AR Tags: Int J Lab Hematol Source Type: research

Development of DNA controls for detection of β-thalassemia mutations commonly found in Asian.
CONCLUSION: The β-globin DNA controls established here provide efficient alternatives to a conventional DNA source from peripheral blood, which is more difficult to obtain. They also provide a platform for future development of β-globin plasmid controls with other mutations, which can also be suitable for other DNA-based approaches. PMID: 32706939 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology)
Source: International Journal of Laboratory Hematology - July 24, 2020 Category: Hematology Authors: Munkongdee T, Nualkaew T, Buasuwan N, Hinna N, Paiboonsukwong K, Sripichai O, Svasti S, Winichagoon P, Fucharoen S, Jearawiriyapaisarn N Tags: Int J Lab Hematol Source Type: research

Does age-adjusted D-dimer have a role in assessment of VTE recurrence rates? Comment.
PMID: 32701216 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology)
Source: International Journal of Laboratory Hematology - July 23, 2020 Category: Hematology Authors: Thomas W, Mindlina I, MacDonald S, Besser M Tags: Int J Lab Hematol Source Type: research

Reflections on the value of the adjective absolute in reference to hematologic indices like absolute neutrophil count.
PMID: 32697424 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology)
Source: International Journal of Laboratory Hematology - July 22, 2020 Category: Hematology Authors: Prakash ES Tags: Int J Lab Hematol Source Type: research

Reference values for extended red blood cell parameters on the Abbott Cell-Dyn sapphire system in Korean adult population.
PMID: 32692909 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology)
Source: International Journal of Laboratory Hematology - July 21, 2020 Category: Hematology Authors: Woo KS, An GD, Lim HH, Han JY Tags: Int J Lab Hematol Source Type: research

International Council for Standardization in Haematology (ICSH) recommendations for laboratory measurement of ADAMTS13.
Abstract This guidance document was prepared on behalf of the International Council for Standardization in Haematology (ICSH), by the ADAMTS13 Assay Working Group, which comprises an international group of both clinical and laboratory experts. The document provides recommendations on best practice for the performance of ADAMTS13 assays in clinical laboratories. ADAMTS13 assays support the differential diagnosis of thrombotic microangiopathies and have utility in the management of thrombotic thrombocytopenic purpura (TTP). There are three types of assay: activity, antigen and autoantibody/inhibitor assays. Methods ...
Source: International Journal of Laboratory Hematology - July 16, 2020 Category: Hematology Authors: Mackie I, Mancini I, Muia J, Kremer Hovinga J, Nair S, Machin S, Baker R Tags: Int J Lab Hematol Source Type: research

Serum folate and cytokines in heterozygous β-thalassemia.
CONCLUSIONS: Mandatory flour fortification with FA in Brazil may be insufficient for β-TmN, since they have higher tHcy and lower serum total folate than controls. Furthermore, β-TmN have higher IL-6 levels than β-TmU. UMFA was not associated with inflammatory cytokine levels. PMID: 32662566 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology)
Source: International Journal of Laboratory Hematology - July 14, 2020 Category: Hematology Authors: Paniz C, Lucena MR, Bertinato JF, Dos Santos MNN, Gomes GW, Figueiredo MS, Sonati MF, Blaia-D Avila VLN, Green R, Guerra-Shinohara EM Tags: Int J Lab Hematol Source Type: research

IgE monoclonal gammopathy: The clinical relevance to perform the immunofixation using IgE antisera.
PMID: 32662590 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology)
Source: International Journal of Laboratory Hematology - July 14, 2020 Category: Hematology Authors: Brivio R, Cappellani A, Carpenedo M, Minolfi V, Intra J, Romano R, Spinoni N, Brambilla P Tags: Int J Lab Hematol Source Type: research

Analysis of hematological indexes of COVID-19 patients from fever clinics in Suzhou, China.
PMID: 32657537 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology)
Source: International Journal of Laboratory Hematology - July 13, 2020 Category: Hematology Authors: Kong J, Wang T, Di Z, Shi B, Yu X, Huang C, Yang Y, Sun H, Yuan D, Wu D, Fu J Tags: Int J Lab Hematol Source Type: research

Comparative sequencing data analysis of Ion Torrent and MinION sequencing platforms using a clinical diagnostic haematology panel.
Abstract INTRODUCTION: Currently, two second-generation sequencing platforms-Ion Torrent and Illumina are being widely used for clinical testing and reporting of human samples. However, third-generation long read platforms like single molecule (PacBio) or direct sequencing (Nanopore) are gaining widespread interest in clinical genomics. AIMS AND OBJECTIVES: The current study attempts to analyse and compare MinION sequencing data with Ion Torrent data, using a haematology ampliseq panel, to shed light on its current standing in reporting of clinical diagnostic samples. METHODOLOGY: A custom targeted Next-...
Source: International Journal of Laboratory Hematology - July 12, 2020 Category: Hematology Authors: Singh A, Bhatia P Tags: Int J Lab Hematol Source Type: research

The traceability of commercial plasma calibrators to the plasma International Standards for factor VIII and factor IX.
This study assessed the relationship between the plasma IS for factors IX (FIX) and VIII (FVIII) and some commonly used commercial calibrators. Calibrators from seven manufacturers were assayed for FIX and FVIII activity by one-stage clotting assay (OSCA) using different activated partial thromboplastin time (APTT) reagents and deficient plasmas, or chromogenic assay (CA). Results were calculated relative to the 4th IS Factors II,VII,IX,X, Plasma or the 6th IS Factor VIII/VWF, Plasma. Results for each calibrator were similar across the APTT reagents and deficient plasmas used. All calibrators showed a recovery of 90%-111% ...
Source: International Journal of Laboratory Hematology - July 8, 2020 Category: Hematology Authors: Wilmot HV, Rakowski K, Gray E Tags: Int J Lab Hematol Source Type: research

A new approach for diagnosing hematological malignancies using monocytosis workflow optimization and abnormal lymphocyte/blast flag of Sysmex XN series of blood count analyzers.
CONCLUSION: Automated validation support systems and softwares developed especially for these systems make it possible to classify patients with their non-specific findings, as a result both contributing to the reduction of laboratory workload and costs and assisting laboratory specialists and clinicians with adding value to laboratory results. PMID: 32639667 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology)
Source: International Journal of Laboratory Hematology - July 8, 2020 Category: Hematology Authors: Kocaturk E, Kusku Kiraz Z, Uskudar Teke H, Demir SS, Alatas IO Tags: Int J Lab Hematol Source Type: research

Use of Sysmex XN-10 red blood cell parameters for screening of hereditary red blood cell diseases and iron deficiency anaemia.
CONCLUSION: Based on the XN-10 RBC and reticulocyte parameters, we propose a two-step decision tree delivering a good prediction and classification of hereditary RBC diseases. These results can be used to optimize additional reticulocyte analysis and microscopy review. PMID: 32639680 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology)
Source: International Journal of Laboratory Hematology - July 8, 2020 Category: Hematology Authors: Nivaggioni V, Bouriche L, Coito S, Le Floch AS, Ibrahim-Kosta M, Leonnet C, Arnoux I, Loosveld M Tags: Int J Lab Hematol Source Type: research

Performance of the Sysmex White Precursor Channel to discover circulating leukemic blast cells.
CONCLUSIONS: WPC blast alarm flags are imprecise and inaccurate, especially when blast counts are low. However, the XN20 will alarm samples with other flags so that most samples containing blast cells will be manually reviewed after all. Hence, the presented flag types should not bias the decisions of manual reviewers. PMID: 32639686 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology)
Source: International Journal of Laboratory Hematology - July 8, 2020 Category: Hematology Authors: Sejrup J, Pedersen DM, Phillipsen JP, Nielsen JØ, Koch SPR, Smith J Tags: Int J Lab Hematol Source Type: research

Molecular and clinical profile of congenital fibrinogen disorders in Iran, identification of two novel mutations.
CONCLUSIONS: In Iran, with its high rate of consanguinity, autosomal recessive afibrinogenemia with severe clinical presentations is relatively common due to heterogeneous molecular defects. PMID: 32639687 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology)
Source: International Journal of Laboratory Hematology - July 8, 2020 Category: Hematology Authors: Tavasoli B, Safa M, Dorgalaleh A, Ghasemi JB, Rezaei Makhouri F, Rezvani MR, Ahmadi A, Tabibian S, Jazebi M, Baghaipor MR, Zaker F Tags: Int J Lab Hematol Source Type: research

Comparison between coagulation factor VIII quantified with one-stage activity assay and with mass spectrometry in haemophilia A patients: Proof of principle.
CONCLUSIONS: Despite good overall correlation between the two methods, relative differences were large, especially for samples with anti-FVIII antibodies or exogenous FVIII products. These differences may have clinical impact. More research is needed to determine the value of FVIII plasma concentration in comparison with FVIII activity. PMID: 32633067 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology)
Source: International Journal of Laboratory Hematology - July 6, 2020 Category: Hematology Authors: Donners AAMT, van Maarseveen EM, Weetink YRJ, El Amrani M, Fischer K, Rademaker CMA, Egberts TCG, Huisman A, Musson REA Tags: Int J Lab Hematol Source Type: research

Recognizing the mobilization of neutrophils with banded nuclei early after trauma.
PMID: 32633074 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology)
Source: International Journal of Laboratory Hematology - July 6, 2020 Category: Hematology Authors: Hellebrekers P, Hesselink L, Huisman A, Ten Berg M, Koenderman L, Leenen LPH, Hietbrink F Tags: Int J Lab Hematol Source Type: research

Keeping the bloodline of haematology medical education flowing during a pandemic.
PMID: 32621530 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology)
Source: International Journal of Laboratory Hematology - July 4, 2020 Category: Hematology Authors: Cheong MA, Chee YL, Ng HJ, Jen WY Tags: Int J Lab Hematol Source Type: research

Pitfalls in CALR exon 9 mutation detection: A single-center experience in 571 positive patients.
CONCLUSION: Our observations showed that every mutation should be verified by direct Sanger sequencing, and we show that sometimes it may be necessary to study germline DNA and to complement with NGS analysis to precisely interpret the molecular alterations. PMID: 32614523 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology)
Source: International Journal of Laboratory Hematology - July 2, 2020 Category: Hematology Authors: Verger E, Maslah N, Schlageter MH, Chomienne C, Kiladjian JJ, Giraudier S, Cassinat B Tags: Int J Lab Hematol Source Type: research

Peripheral lymphocyte subset alterations in COVID-19 patients.
PMID: 32608572 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology)
Source: International Journal of Laboratory Hematology - July 1, 2020 Category: Hematology Authors: Chan SSW, Christopher D, Tan GB, Chong VCL, Fan BE, Lin CY, Ong KH Tags: Int J Lab Hematol Source Type: research

Manifestations of blood coagulation and its relation to clinical outcomes in severe COVID-19 patients: Retrospective analysis.
Abstract INTRODUCTION: Characteristics of blood coagulation and its relation to clinical outcomes in COVID-19 patients are still rarely reported. We aimed to investigate the blood coagulation function and its influences on clinical outcomes of patients with syndrome coronavirus 2 (SARS-CoV-2) infection. METHODS: A total of 71 severe patients with confirmed SARS-CoV-2 infection who were treated in Wuhan First Hospital from February 12 to March 20, 2020, were enrolled. The blood coagulation data in these patients and in 61 healthy controls were collected. The patients with COVID-19 were divided into two groups:...
Source: International Journal of Laboratory Hematology - June 27, 2020 Category: Hematology Authors: Zhang Y, He L, Chen H, Lu S, Xiong Y, Liu J, Zheng Y, Wang S, Liu L Tags: Int J Lab Hematol Source Type: research

Improving performance of recently introduced flow cytometry-based approach of malignant cell screening in serous cavity effusion.
CONCLUSIONS: Serous cavity effusion will be increasingly analyzed using the automated hematology analyzer BF mode in the future because of its rapidness and convenience. The combined application of HFC with scattergram rules is a feasible and useful approach to screen malignant cells in serous cavity effusion. PMID: 32573949 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology)
Source: International Journal of Laboratory Hematology - June 23, 2020 Category: Hematology Authors: Sun J, Ding S, Zhu L, Liu Y, Jiang Q, Song S, Chen W, Li S, Ma P Tags: Int J Lab Hematol Source Type: research

Evaluation of DOAC Filter, a new device to remove direct oral anticoagulants from plasma samples.
CONCLUSION: Directs oral anticoagulants Filter efficiently removes DOACs from plasma and achieves concentrations below DOAC-specific assays LoD, except in the case of one apixaban sample. The integrity of plasma is respected, and the cartridge seems not to impact LA diagnosis. PMID: 32567799 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology)
Source: International Journal of Laboratory Hematology - June 22, 2020 Category: Hematology Authors: Sevenet PO, Cucini V, Hervé T, Depasse F, Carlo A, Contant G, Mathieu O Tags: Int J Lab Hematol Source Type: research

Screening on platelet LncRNA expression profile discloses novel residual platelet reactivity biomarker.
CONCLUSION: There is a considerable amount of lncRNAs in platelets, and the downregulated expression of ENST00000433442 is an independent risk factor for high residual platelet reactivity in CHD patients under DAPT. PMID: 32567809 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology)
Source: International Journal of Laboratory Hematology - June 22, 2020 Category: Hematology Authors: Yan S, Liu X, Ke X, Xian Z, Peng C, Wang X, Chen M Tags: Int J Lab Hematol Source Type: research

Application of HbA2 levels and red cell indices-based new model in the differentiation of thalassemia traits from iron deficiency in hypochromic microcytic anemia Cases.
Abstract INTRODUCTION: Thalassemia traits and iron deficiency anemia are the most common types of hypochromic microcytic anemia with similar clinical and laboratory features. It is vital to establish a new screening model based on HbA2 levels and red cell indices for the differentiation of TT from IDA in hypochromic microcytic anemia cases. METHOD: The data comprised of the red blood cell indices and HbA2 prenatal diagnostic test results of 810 individuals who were identified to conform to the following criteria: MCV 
Source: International Journal of Laboratory Hematology - June 21, 2020 Category: Hematology Authors: Zhou Y, Zhang J, Wang C, Zhou L, Zhou L, Ou D, Peng D Tags: Int J Lab Hematol Source Type: research

Systematic review about etiologic association to the leukoerythroblastic reaction.
CONCLUSION: The literature on LER is scarce and heterogeneous. The etiological factors of LER are diverse, and its presence in malignant disease is an indicator of disease progression and an adverse prognosis suggesting poor survival. In those cases where LER had neither hematological nor solid neoplasms, its manifestation, prognosis and its impact on our daily clinical practice are unknown. PMID: 32562368 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology)
Source: International Journal of Laboratory Hematology - June 20, 2020 Category: Hematology Authors: Tabares Calvache E, Tabares Calvache AD, Faulhaber GAM Tags: Int J Lab Hematol Source Type: research

Two homozygous missense mutations in ITGB3 gene as a cause of Glanzmann Thrombasthenia in four consanguineous Pakistani pedigrees.
CONCLUSION: This study provides an initial yet important account of clinical and genetic characterization of GT in local patients which may spark further studies to help molecular diagnosis, optimal disease management, and genetic counseling based prevention efforts. PMID: 32558238 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology)
Source: International Journal of Laboratory Hematology - June 19, 2020 Category: Hematology Authors: Ali T, Gul S, Amar A, Shakoor M, Farhan S, Mohsin S, Khaliq S Tags: Int J Lab Hematol Source Type: research

Assessment of Reticulocyte hemoglobin content in infants in intensive care unities.
PMID: 32558240 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology)
Source: International Journal of Laboratory Hematology - June 19, 2020 Category: Hematology Authors: Dzirba TA, Moss MF, Dionisio LM Tags: Int J Lab Hematol Source Type: research

Prevalence of myeloperoxidase deficiency determined using an ADVIA 2120i.
PMID: 32543762 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology)
Source: International Journal of Laboratory Hematology - June 16, 2020 Category: Hematology Authors: Gleghorn DM, Thomas W Tags: Int J Lab Hematol Source Type: research

Cross-evaluation of five slidemakers and three automated image analysis systems: The pitfalls of automation?
This study evaluates the integrated performance of slidemakers and digital microscopes commonly available on the market. METHODS: We compared the percentage of neutrophils for five slidemakers (two Siemens Advia Autoslides, a SysmexSP-10 and SP-50 and an Abbot Alinity hs) and a Horiba Hemaprep to the corresponding haematology analyser data (Siemens Advia 2120i, Sysmex XN and Abbot Alinity hq). Differential leucocyte counting (DLC) was performed on three different CellaVision digital microscopes (DM96, DM1200 and DI-60) and manually. The quality of the smears was assessed using a CellaVision SmearChecker. RESULTS:...
Source: International Journal of Laboratory Hematology - June 15, 2020 Category: Hematology Authors: Saad Albichr I, Sottiaux JY, Hotton J, De Laveleye M, Dupret P, Detry G Tags: Int J Lab Hematol Source Type: research

Hemoglobin-bound platelets correlate with the increased platelet activity in hemoglobin E/ β-thalassemia.
CONCLUSION: HbE/β-thal patients have increased Hb-bound platelets, which is associated with increased baseline platelet activation and reactivity. PMID: 32539231 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology)
Source: International Journal of Laboratory Hematology - June 15, 2020 Category: Hematology Authors: Chamchoi A, Srihirun S, Paiboonsukwong K, Sriwantana T, Kongkaew P, Fucharoen S, Pattanapanyasat K, Sibmooh N Tags: Int J Lab Hematol Source Type: research

Comparing the area under the curve and peak height methods in the calculation of FLT3-ITD allelic ratio.
PMID: 32533751 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology)
Source: International Journal of Laboratory Hematology - June 13, 2020 Category: Hematology Authors: Di Ciaccio P, Kearney A, Ling S Tags: Int J Lab Hematol Source Type: research

Signet ring cell carcinocythaemia in an advanced gastric carcinoma.
J, Merino A PMID: 32501616 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology)
Source: International Journal of Laboratory Hematology - June 5, 2020 Category: Hematology Authors: Rakislova N, Cuatrecasas M, Molina A, Rodrigo M, Moreira L, Laguna J, Rodríguez M, Pérez FM, Carlos Laguna J, Merino A Tags: Int J Lab Hematol Source Type: research

Differential expression of miRNAs and their target genes: Exploring a new perspective of acquired aplastic anemia pathogenesis.
CONCLUSION: This is the first study from the Indian subcontinent demonstrating the presence of altered miRNA expression in the bone marrow samples of aAA patients, suggesting their role in the pathogenesis of the disease. A comprehensive study focusing on the effect of these miRNA-mRNA interactions is likely to open new avenues of management. PMID: 32490599 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology)
Source: International Journal of Laboratory Hematology - June 3, 2020 Category: Hematology Authors: Srivastava J, Chaturvedi CP, Rahman K, Gupta R, Sharma A, Chandra D, Singh MK, Gupta A, Yadav S, Nityanand S Tags: Int J Lab Hematol Source Type: research

Assessment of time-dependent white blood cells degeneration induced by blood storage on automated parameters and morphology examination.
PMID: 32475017 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology)
Source: International Journal of Laboratory Hematology - May 31, 2020 Category: Hematology Authors: Naoum FA, Martin FHO, Valejo MR, Oliveira MGL Tags: Int J Lab Hematol Source Type: research

Mutant specific anti calreticulin antibody (CAL2) immunohistochemistry as a screening test for calreticulin (CALR) mutation testing.
Abstract BACKGROUND: About 50 different CALR frameshift mutations have been identified in BCR-ABL1 negative MPN, all leading to the development of common new protein C terminus. Antibody targeting this terminal epitope can be useful to identify this driver mutation using immunohistochemistry. MATERIALS AND METHODS: CALR mutation analysis was carried out in 51 JAK2V617F negative cases, PMF (n = 22) and ET (n = 29). PCR followed by fragment analysis was performed for molecular detection of CALR mutation. Bone marrow biopsy specimens of corresponding patients were subjected to IHC using mutat...
Source: International Journal of Laboratory Hematology - May 28, 2020 Category: Hematology Authors: Rahman K, Chandra D, Singh MK, Gupta R, Sharma A, Paul P, Kumar S, Sharma S, Nityanand S Tags: Int J Lab Hematol Source Type: research

P-glycoprotein and multidrug resistance-associated protein-1 expression in acute myeloid leukemia: Biological and prognosis implications.
CONCLUSIONS: The results showed that the detection of MDR phenotype by flow cytometry can be a molecular marker for prognosis of patients with AML. PMID: 32452631 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology)
Source: International Journal of Laboratory Hematology - May 26, 2020 Category: Hematology Authors: da Silveira Júnior LS, Soares VL, Jardim da Silva AS, Gil EA, Pereira de Araújo MDG, Merces Gonçalves CA, Paiva AS, Moura de Oliveira TM, Oliveira GHM, Kramer Cavacanti E Silva DG, de Araújo Moura Lemos TM, Moretti Rebecchi IM, de Farias Sales VS, Luc Tags: Int J Lab Hematol Source Type: research

FLAER-negative CD15+ neutrophils can be used for the simplified screening of suspected PNH cases.
CONCLUSION: This simple two-color evaluation of FLAER-negative neutrophils is a highly effective screening test for PNH. Although this approach is not intended to replace the multicolor diagnostic procedure, it could minimize the number of patients requiring a conventional multicolor flow cytometric assay. PMID: 32449605 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology)
Source: International Journal of Laboratory Hematology - May 25, 2020 Category: Hematology Authors: Semchenkova A, Kashpor S, Verzhbitskaya T, Borisov V, Illarionova O, Fechina L, Maschan A, Novichkova G, Popov A Tags: Int J Lab Hematol Source Type: research

Brief communication: Distribution of bone marrow cell subsets and hemodilution in patients with acute leukemia.
gue M PMID: 32449827 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology)
Source: International Journal of Laboratory Hematology - May 25, 2020 Category: Hematology Authors: Gener G, Espasa A, Raya M, Vergara S, Juncà J, Sorigue M Tags: Int J Lab Hematol Source Type: research

Involvement of ADAMTS13 and von Willebrand factor in thromboembolic events in patients infected with SARS-CoV-2.
PMID: 32441844 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology)
Source: International Journal of Laboratory Hematology - May 22, 2020 Category: Hematology Authors: Huisman A, Beun R, Sikma M, Westerink J, Kusadasi N Tags: Int J Lab Hematol Source Type: research

Evaluation of a new thromboplastin reagent STA-NeoPTimal on a STA R Max analyzer for the measurement of prothrombin time, international normalized ratio and extrinsic factor levels.
CONCLUSION: The STA-NeoPTimal showed comparable performance to ReadiPlasTin, making it suitable for VKA control, detection of factors II, V, VII, X deficiency and assessment of liver disease coagulopathy. However, for patients receiving VKA, some significant differences were observed. We confirmed the inability of the PT assay to detect residual DOAC concentrations. Finally, burned patients results showed that recombinant thromboplastins were less sensitive to factor deficiencies in comparison to extraction thromboplastins. PMID: 32426926 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology)
Source: International Journal of Laboratory Hematology - May 19, 2020 Category: Hematology Authors: Mullier F, Paridaens MS, Evrard J, Baudar J, Guldenpfennig M, Devroye C, Miller L, Chatelain B, Lessire S, Jacqmin H Tags: Int J Lab Hematol Source Type: research

Evaluation of automated capillary complete blood counts for routine clinical decision making in a large cohort of hematological patients, using Mindray BC-3000 Plus Auto and Sysmex XE-5000 hematology analyzers.
CONCLUSION: Capillary blood and VB counts in hematological patients were equivalent for most basic hematological parameters. Hb, ANC, and Plt CB counts revealed clinically significant performance, indicating that they can reliably substitute VB sampling in the day work. PMID: 32426940 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology)
Source: International Journal of Laboratory Hematology - May 19, 2020 Category: Hematology Authors: Oudatzis G, Tsagarakis NJ, Paterakis G, Vasileiou P, Xenou E, Maraki P, Pangrati T, Angelopoulou MK, Vassilakopoulos TP, Konstantopoulos K Tags: Int J Lab Hematol Source Type: research

Search for a practical approach for detection of clopidogrel resistance: Comparison of light transmission aggregometry and INNOVANCE ® PFA P2Y cartridge and correlation with CYP2C19 variants.
Search for a practical approach for detection of clopidogrel resistance: Comparison of light transmission aggregometry and INNOVANCE® PFA P2Y cartridge and correlation with CYP2C19 variants. Int J Lab Hematol. 2020 May 19;: Authors: Heireman L, Monteyne T, Hemelsoet D, van Schaik RHN, Devreese KMJ PMID: 32427413 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology)
Source: International Journal of Laboratory Hematology - May 19, 2020 Category: Hematology Authors: Heireman L, Monteyne T, Hemelsoet D, van Schaik RHN, Devreese KMJ Tags: Int J Lab Hematol Source Type: research

Prevalence of globin gene modifiers encountered in fetuses during antenatal diagnosis of hemoglobinopathies.
CONCLUSION: Many fetuses had one or two gene modifiers. However, the impact of these on ameliorating the severity of the disease could not be evaluated as all the fetuses with β thalassemia major or sickle cell disease were terminated. Parents having heterozygous fetuses with α gene triplication should be followed up periodically after birth for better management of these babies. PMID: 32412692 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology)
Source: International Journal of Laboratory Hematology - May 15, 2020 Category: Hematology Authors: Mehta P, Sawant P, Gorivale M, Nadkarni A, Colah R, Mukherjee MB Tags: Int J Lab Hematol Source Type: research

Evans syndrome in a female with erythrophagocytosis by monocytes in peripheral blood smear.
PMID: 32383790 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology)
Source: International Journal of Laboratory Hematology - May 8, 2020 Category: Hematology Authors: Lian X, Liu C, Hou X, Zhang W, Hao J Tags: Int J Lab Hematol Source Type: research

Significance of CD10 protein expression in the diagnostics of follicular lymphoma: A comparison of conventional immunohistochemistry with flow cytometry supported by the establishment of BCL2 and BCL6 rearrangements.
CONCLUSIONS: Immunohistochemistry staining is a comparable method to FCM assessment in the evaluation of CD10 expression and diagnosis of FL. Fine-needle aspiration biopsy/FCM (FNAB/FCM) could be a useful tool for verifying FL diagnosis and CD10 detection. Despite its heterogeneity, FL has a characteristic immunophenotype. BCL2R and BCL6R FL cases differ mainly in levels of BCL2 and CD10 with CD43 co-expression; BCL2(+)higher by FCM correlates with BCL2R. Moreover, FNAB plays an important role in material provision for supportive karyotyping and BCL2R, BCL6R assessed by FISH. PMID: 32364682 [PubMed - as supplied by pu...
Source: International Journal of Laboratory Hematology - May 4, 2020 Category: Hematology Authors: Szumera-Ciećkiewicz A, Rymkiewicz G, Sokół K, Paszkiewicz-Kozik E, Borysiuk A, Poleszczuk J, Bachnio K, Bystydzienski Z, Woroniecka R, Grygalewicz B, Kotarska M, Stańczak M, Owczarek D, Pytlak B, Prochorec-Sobieszek M, Walewski J Tags: Int J Lab Hematol Source Type: research

The antiphosphatidylserine/prothrombin detection in solid phase assay is largely dependent on the type of samples.
PMID: 32359016 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology)
Source: International Journal of Laboratory Hematology - May 2, 2020 Category: Hematology Authors: Kumano O, Ieko M, Yoshida M, Naito S, Ohmura K, Takahashi N Tags: Int J Lab Hematol Source Type: research

Identification of acquired PIGA mutations and additional variants by next-generation sequencing in paroxysmal nocturnal hemoglobinuria.
CONCLUSION: The detection of PIGA mutations and additional variants by targeted NGS not only shed light on the genetic characteristics of PNH, but also provided an important reference value in the diagnosis of PNH at molecular level. PMID: 32359022 [PubMed - as supplied by publisher] (Source: International Journal of Laboratory Hematology)
Source: International Journal of Laboratory Hematology - May 2, 2020 Category: Hematology Authors: Li J, Lin Y, Chen L, Qin L, Tan H, Zou J, Zhang D, Nie Y, Wang G, Zhang H, Liu E, Chen X, Ru K Tags: Int J Lab Hematol Source Type: research