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Rapid, automated quantification of Haemonchus contortus ova in sheep faecal samples
In this study, 74 fecal samples were collected from sheep and analyzed for PNA-stained H. contortus, using both PS and manual fluorescence microscopy. The percentage of H. contortus was determined based on standard total strongylid counts with PS or brightfield microscopy. Additionally, 15 samples were processed for coproculture with larval identification, and analyzed with both manual and automated PNA methods. All methods were compared using the coefficient of determination (R2) and the Lin's concordance correlation coefficient (ρc). ParasightTM and manual PNA percent H. contortus results were highly correlated with R2=...
Source: International Journal for Parasitology - August 16, 2023 Category: Parasitology Authors: Jennifer L Cain Leonor Sicalo Gianechini Abigail L Vetter Sarah M Davis Leah N Britton Jennifer L Myka Paul Slusarewicz Source Type: research

A mixed amplicon metabarcoding and sequencing approach for surveillance of drug resistance to levamisole and benzimidazole in Haemonchus spp
We describe a mixed deep amplicon sequencing approach to determine the frequency of the levamisole (LEV)-resistant single nucleotide polymorphism (SNP) within arc-8 exon 4 (S168T) in Haemonchus spp., coupled with benzimidazole (BZ)-resistant SNPs within β-tubulin isotype-1 and the internal transcribed spacer-2 (ITS-2) nemabiome. This constitutes the first known multi-drug and multi-species molecular diagnostic developed for helminths of veterinary importance. Of the ovine, bovine, caprine and camelid Australian field isolates we tested, S168T was detected in the majority of Haemonchus spp. populations from sheep and goats...
Source: International Journal for Parasitology - August 3, 2023 Category: Parasitology Authors: Emily Kate Francis Alistair Antonopoulos Mark Edward Westman Janina McKay-Demeler Roz Laing Jan Šlapeta Source Type: research

Validation of a serum ELISA test for cyathostomin infection in equines
In conclusion, the CT3 test provides an option for veterinarians to obtain evidence of low cyathostomin burdens that do not require anthelmintic treatment and to support diagnosis of infection.PMID:37536388 | DOI:10.1016/j.ijpara.2023.07.001 (Source: International Journal for Parasitology)
Source: International Journal for Parasitology - August 3, 2023 Category: Parasitology Authors: Kirsty L Lightbody Andrew Austin Peter A Lambert Georg von Samson-Himmelstjerna Laura J ürgenschellert J ürgen Krücken Martin K Nielsen Guillaume Sall é Fabrice Reigner Callum G Donnelly Carrie J Finno Nicola Walshe Grace Mulcahy Nicola Housby-Skeggs Source Type: research

A mixed amplicon metabarcoding and sequencing approach for surveillance of drug resistance to levamisole and benzimidazole in Haemonchus spp
We described a mixed deep amplicon sequencing approach to determine the frequency of the levamisole (LEV)-resistant single nucleotide polymorphism (SNP) within arc-8 exon 4 (S168T) in Haemonchus spp., coupled with benzimidazole (BZ)-resistant SNPs within β-tubulin isotype-1 and the internal transcribed spacer-2 (ITS-2) nemabiome. This constitutes the first known multi-drug and multi-species molecular diagnostic developed for helminths of veterinary importance. Of the ovine, bovine, caprine and camelid Australian field isolates we tested, S168T was detected in the majority of Haemonchus spp. populations from sheep and goat...
Source: International Journal for Parasitology - August 3, 2023 Category: Parasitology Authors: Emily Kate Francis Alistair Antonopoulos Mark Edward Westman Janina McKay-Demeler Roz Laing Jan Šlapeta Source Type: research