Rapid, automated quantification of Haemonchus contortus ova in sheep faecal samples

In this study, 74 fecal samples were collected from sheep and analyzed for PNA-stained H. contortus, using both PS and manual fluorescence microscopy. The percentage of H. contortus was determined based on standard total strongylid counts with PS or brightfield microscopy. Additionally, 15 samples were processed for coproculture with larval identification, and analyzed with both manual and automated PNA methods. All methods were compared using the coefficient of determination (R2) and the Lin's concordance correlation coefficient (ρc). ParasightTM and manual PNA percent H. contortus results were highly correlated with R2=0.8436 and ρc=0.9100 for all 74 fecal samples. Coproculture versus PS percent H. contortus were also highly correlated with R2=0.8245 and ρc=0.8605. Overall, this system provides a rapid and convenient method for determining the percentage of H. contortus in sheep and goat fecal samples without requiring specialized training.PMID:37586585 | DOI:10.1016/j.ijpara.2023.07.003
Source: International Journal for Parasitology - Category: Parasitology Authors: Source Type: research