A mixed amplicon metabarcoding and sequencing approach for surveillance of drug resistance to levamisole and benzimidazole in Haemonchus spp
We described a mixed deep amplicon sequencing approach to determine the frequency of the levamisole (LEV)-resistant single nucleotide polymorphism (SNP) within arc-8 exon 4 (S168T) in Haemonchus spp., coupled with benzimidazole (BZ)-resistant SNPs within β-tubulin isotype-1 and the internal transcribed spacer-2 (ITS-2) nemabiome. This constitutes the first known multi-drug and multi-species molecular diagnostic developed for helminths of veterinary importance. Of the ovine, bovine, caprine and camelid Australian field isolates we tested, S168T was detected in the majority of Haemonchus spp. populations from sheep and goat...
Source: International Journal for Parasitology - August 3, 2023 Category: Parasitology Authors: Emily Kate Francis Alistair Antonopoulos Mark Edward Westman Janina McKay-Demeler Roz Laing Jan Šlapeta Source Type: research

Validation of a serum ELISA test for cyathostomin infection in equines
In conclusion, the CT3 test provides an option for veterinarians to obtain evidence of low cyathostomin burdens that do not require anthelmintic treatment and to support diagnosis of infection.PMID:37536388 | DOI:10.1016/j.ijpara.2023.07.001 (Source: International Journal for Parasitology)
Source: International Journal for Parasitology - August 3, 2023 Category: Parasitology Authors: Kirsty L Lightbody Andrew Austin Peter A Lambert Georg von Samson-Himmelstjerna Laura J ürgenschellert J ürgen Krücken Martin K Nielsen Guillaume Sall é Fabrice Reigner Callum G Donnelly Carrie J Finno Nicola Walshe Grace Mulcahy Nicola Housby-Skeggs Source Type: research

A mixed amplicon metabarcoding and sequencing approach for surveillance of drug resistance to levamisole and benzimidazole in Haemonchus spp
We described a mixed deep amplicon sequencing approach to determine the frequency of the levamisole (LEV)-resistant single nucleotide polymorphism (SNP) within arc-8 exon 4 (S168T) in Haemonchus spp., coupled with benzimidazole (BZ)-resistant SNPs within β-tubulin isotype-1 and the internal transcribed spacer-2 (ITS-2) nemabiome. This constitutes the first known multi-drug and multi-species molecular diagnostic developed for helminths of veterinary importance. Of the ovine, bovine, caprine and camelid Australian field isolates we tested, S168T was detected in the majority of Haemonchus spp. populations from sheep and goat...
Source: International Journal for Parasitology - August 3, 2023 Category: Parasitology Authors: Emily Kate Francis Alistair Antonopoulos Mark Edward Westman Janina McKay-Demeler Roz Laing Jan Šlapeta Source Type: research

Validation of a serum ELISA test for cyathostomin infection in equines
In conclusion, the CT3 test provides an option for veterinarians to obtain evidence of low cyathostomin burdens that do not require anthelmintic treatment and to support diagnosis of infection.PMID:37536388 | DOI:10.1016/j.ijpara.2023.07.001 (Source: International Journal for Parasitology)
Source: International Journal for Parasitology - August 3, 2023 Category: Parasitology Authors: Kirsty L Lightbody Andrew Austin Peter A Lambert Georg von Samson-Himmelstjerna Laura J ürgenschellert J ürgen Krücken Martin K Nielsen Guillaume Sall é Fabrice Reigner Callum G Donnelly Carrie J Finno Nicola Walshe Grace Mulcahy Nicola Housby-Skeggs Source Type: research

A mixed amplicon metabarcoding and sequencing approach for surveillance of drug resistance to levamisole and benzimidazole in Haemonchus spp
We described a mixed deep amplicon sequencing approach to determine the frequency of the levamisole (LEV)-resistant single nucleotide polymorphism (SNP) within arc-8 exon 4 (S168T) in Haemonchus spp., coupled with benzimidazole (BZ)-resistant SNPs within β-tubulin isotype-1 and the internal transcribed spacer-2 (ITS-2) nemabiome. This constitutes the first known multi-drug and multi-species molecular diagnostic developed for helminths of veterinary importance. Of the ovine, bovine, caprine and camelid Australian field isolates we tested, S168T was detected in the majority of Haemonchus spp. populations from sheep and goat...
Source: International Journal for Parasitology - August 3, 2023 Category: Parasitology Authors: Emily Kate Francis Alistair Antonopoulos Mark Edward Westman Janina McKay-Demeler Roz Laing Jan Šlapeta Source Type: research

Validation of a serum ELISA test for cyathostomin infection in equines
In conclusion, the CT3 test provides an option for veterinarians to obtain evidence of low cyathostomin burdens that do not require anthelmintic treatment and to support diagnosis of infection.PMID:37536388 | DOI:10.1016/j.ijpara.2023.07.001 (Source: International Journal for Parasitology)
Source: International Journal for Parasitology - August 3, 2023 Category: Parasitology Authors: Kirsty L Lightbody Andrew Austin Peter A Lambert Georg von Samson-Himmelstjerna Laura J ürgenschellert J ürgen Krücken Martin K Nielsen Guillaume Sall é Fabrice Reigner Callum G Donnelly Carrie J Finno Nicola Walshe Grace Mulcahy Nicola Housby-Skeggs Source Type: research

A mixed amplicon metabarcoding and sequencing approach for surveillance of drug resistance to levamisole and benzimidazole in Haemonchus spp
We described a mixed deep amplicon sequencing approach to determine the frequency of the levamisole (LEV)-resistant single nucleotide polymorphism (SNP) within arc-8 exon 4 (S168T) in Haemonchus spp., coupled with benzimidazole (BZ)-resistant SNPs within β-tubulin isotype-1 and the internal transcribed spacer-2 (ITS-2) nemabiome. This constitutes the first known multi-drug and multi-species molecular diagnostic developed for helminths of veterinary importance. Of the ovine, bovine, caprine and camelid Australian field isolates we tested, S168T was detected in the majority of Haemonchus spp. populations from sheep and goat...
Source: International Journal for Parasitology - August 3, 2023 Category: Parasitology Authors: Emily Kate Francis Alistair Antonopoulos Mark Edward Westman Janina McKay-Demeler Roz Laing Jan Šlapeta Source Type: research

Validation of a serum ELISA test for cyathostomin infection in equines
In conclusion, the CT3 test provides an option for veterinarians to obtain evidence of low cyathostomin burdens that do not require anthelmintic treatment and to support diagnosis of infection.PMID:37536388 | DOI:10.1016/j.ijpara.2023.07.001 (Source: International Journal for Parasitology)
Source: International Journal for Parasitology - August 3, 2023 Category: Parasitology Authors: Kirsty L Lightbody Andrew Austin Peter A Lambert Georg von Samson-Himmelstjerna Laura J ürgenschellert J ürgen Krücken Martin K Nielsen Guillaume Sall é Fabrice Reigner Callum G Donnelly Carrie J Finno Nicola Walshe Grace Mulcahy Nicola Housby-Skeggs Source Type: research

German Ixodes inopinatus samples may not actually represent this tick species
Int J Parasitol. 2023 Jul 27:S0020-7519(23)00157-1. doi: 10.1016/j.ijpara.2023.06.007. Online ahead of print.ABSTRACTTicks are important vectors of human and animal pathogens, but many questions remain unanswered regarding their taxonomy. Molecular sequencing methods have allowed research to start understanding the evolutionary history of even closely related tick species. Ixodes inopinatus is considered a sister species and highly similar to Ixodes ricinus, an important vector of many tick-borne pathogens in Europe, but identification between these species remains ambiguous with disagreement on the geographic extent of I....
Source: International Journal for Parasitology - July 29, 2023 Category: Parasitology Authors: Robert E Rollins Gabriele Margos Andreas Brachmann Stefan Krebs Alexia Mouchet Niels J Dingemanse AbdElkarim Laatamna Nassiba Reghaissia Volker Fingerle Dirk Metzler No émie S Becker Lidia Chitimia-Dobler Source Type: research

German Ixodes inopinatus samples may not actually represent this tick species
Int J Parasitol. 2023 Jul 27:S0020-7519(23)00157-1. doi: 10.1016/j.ijpara.2023.06.007. Online ahead of print.ABSTRACTTicks are important vectors of human and animal pathogens, but many questions remain unanswered regarding their taxonomy. Molecular sequencing methods have allowed research to start understanding the evolutionary history of even closely related tick species. Ixodes inopinatus is considered a sister species and highly similar to Ixodes ricinus, an important vector of many tick-borne pathogens in Europe, but identification between these species remains ambiguous with disagreement on the geographic extent of I....
Source: International Journal for Parasitology - July 29, 2023 Category: Parasitology Authors: Robert E Rollins Gabriele Margos Andreas Brachmann Stefan Krebs Alexia Mouchet Niels J Dingemanse AbdElkarim Laatamna Nassiba Reghaissia Volker Fingerle Dirk Metzler No émie S Becker Lidia Chitimia-Dobler Source Type: research

German Ixodes inopinatus samples may not actually represent this tick species
Int J Parasitol. 2023 Jul 27:S0020-7519(23)00157-1. doi: 10.1016/j.ijpara.2023.06.007. Online ahead of print.ABSTRACTTicks are important vectors of human and animal pathogens, but many questions remain unanswered regarding their taxonomy. Molecular sequencing methods have allowed research to start understanding the evolutionary history of even closely related tick species. Ixodes inopinatus is considered a sister species and highly similar to Ixodes ricinus, an important vector of many tick-borne pathogens in Europe, but identification between these species remains ambiguous with disagreement on the geographic extent of I....
Source: International Journal for Parasitology - July 29, 2023 Category: Parasitology Authors: Robert E Rollins Gabriele Margos Andreas Brachmann Stefan Krebs Alexia Mouchet Niels J Dingemanse AbdElkarim Laatamna Nassiba Reghaissia Volker Fingerle Dirk Metzler No émie S Becker Lidia Chitimia-Dobler Source Type: research

German Ixodes inopinatus samples may not actually represent this tick species
Int J Parasitol. 2023 Jul 27:S0020-7519(23)00157-1. doi: 10.1016/j.ijpara.2023.06.007. Online ahead of print.ABSTRACTTicks are important vectors of human and animal pathogens, but many questions remain unanswered regarding their taxonomy. Molecular sequencing methods have allowed research to start understanding the evolutionary history of even closely related tick species. Ixodes inopinatus is considered a sister species and highly similar to Ixodes ricinus, an important vector of many tick-borne pathogens in Europe, but identification between these species remains ambiguous with disagreement on the geographic extent of I....
Source: International Journal for Parasitology - July 29, 2023 Category: Parasitology Authors: Robert E Rollins Gabriele Margos Andreas Brachmann Stefan Krebs Alexia Mouchet Niels J Dingemanse AbdElkarim Laatamna Nassiba Reghaissia Volker Fingerle Dirk Metzler No émie S Becker Lidia Chitimia-Dobler Source Type: research

German Ixodes inopinatus samples may not actually represent this tick species
Int J Parasitol. 2023 Jul 27:S0020-7519(23)00157-1. doi: 10.1016/j.ijpara.2023.06.007. Online ahead of print.ABSTRACTTicks are important vectors of human and animal pathogens, but many questions remain unanswered regarding their taxonomy. Molecular sequencing methods have allowed research to start understanding the evolutionary history of even closely related tick species. Ixodes inopinatus is considered a sister species and highly similar to Ixodes ricinus, an important vector of many tick-borne pathogens in Europe, but identification between these species remains ambiguous with disagreement on the geographic extent of I....
Source: International Journal for Parasitology - July 29, 2023 Category: Parasitology Authors: Robert E Rollins Gabriele Margos Andreas Brachmann Stefan Krebs Alexia Mouchet Niels J Dingemanse AbdElkarim Laatamna Nassiba Reghaissia Volker Fingerle Dirk Metzler No émie S Becker Lidia Chitimia-Dobler Source Type: research

Morphological constraint obscures richness: a mitochondrial exploration of cryptic richness in Transversotrema (Trematoda: Transversotrematidae)
Int J Parasitol. 2023 Jul 22:S0020-7519(23)00156-X. doi: 10.1016/j.ijpara.2023.06.006. Online ahead of print.ABSTRACTSpecies of Transversotrema Witenberg, 1944 (Transversotrematidae) occupy a unique ecological niche for the Trematoda, living externally under the scales of their teleost hosts. Previous studies of the genus have been impeded partly by limited variation in ribosomal DNA sequence data between closely related species and partly by a lack of morphometrically informative characters. Here, we assess richness of the tropical Indo-west Pacific species through parallel phylogenetic and morphometric analyses, generati...
Source: International Journal for Parasitology - July 24, 2023 Category: Parasitology Authors: Scott C Cutmore Richard D Corner Thomas H Cribb Source Type: research

Morphological constraint obscures richness: a mitochondrial exploration of cryptic richness in Transversotrema (Trematoda: Transversotrematidae)
Int J Parasitol. 2023 Jul 22:S0020-7519(23)00156-X. doi: 10.1016/j.ijpara.2023.06.006. Online ahead of print.ABSTRACTSpecies of Transversotrema Witenberg, 1944 (Transversotrematidae) occupy a unique ecological niche for the Trematoda, living externally under the scales of their teleost hosts. Previous studies of the genus have been impeded partly by limited variation in ribosomal DNA sequence data between closely related species and partly by a lack of morphometrically informative characters. Here, we assess richness of the tropical Indo-west Pacific species through parallel phylogenetic and morphometric analyses, generati...
Source: International Journal for Parasitology - July 24, 2023 Category: Parasitology Authors: Scott C Cutmore Richard D Corner Thomas H Cribb Source Type: research