Isolation of Toxoplasma gondii in cell culture: an alternative to bioassay
Int J Parasitol. 2023 Dec 12:S0020-7519(23)00228-X. doi: 10.1016/j.ijpara.2023.12.002. Online ahead of print.ABSTRACTToxoplasma gondii is an apicomplexan protozoan parasite that can infect mammals and birds. The infection can cause acute toxoplasmosis and death in susceptible hosts. Bioassay using cats and mice has been the standard for the isolation of T. gondi from infected hosts for the past several decades. However, bioassay is labor-intensive, expensive, and involves using laboratory animals. To search alternative approaches and to work towards replacement of animal experiments, we summarized the key literature and co...
Source: International Journal for Parasitology - December 14, 2023 Category: Parasitology Authors: Tania Dawant Wei Wang Maria Spriggs Geraldo Magela de Faria Junior Laura Horton Nicole M Szafranski Helga Waap Pikka Jokelainen Richard W Gerhold Chunlei Su Source Type: research

A Taq-man-based multiplex quantitative PCR for the simultaneous detection and quantification of Angiostrongylus vasorum, Crenosoma vulpis, and species of respiratory capillarids in canids
Int J Parasitol. 2023 Dec 12:S0020-7519(23)00227-8. doi: 10.1016/j.ijpara.2023.12.001. Online ahead of print.ABSTRACTIn recent years, Angiostrongylus vasorum, Crenosoma vulpis, Eucoleus aerophilus (syn. Capillaria aerophila) and Eucoleus boehmi (syn. Capillaria boehmi), commonly referred to as canine lungworms, have gained a growing interest worldwide as the result of their geographical expansion. Each of these nematode species differs considerably in its biology and pathogenicity. Despite their impact on dogs' health, these parasites are often underdiagnosed owing to diagnostic challenges. Here, we describe the developmen...
Source: International Journal for Parasitology - December 14, 2023 Category: Parasitology Authors: Luca Massetti Manuela Schnyder Anke Wiethoelter Emanuele Brianti Phillip McDonagh Rebecca Traub Vito Colella Source Type: research

Isolation of Toxoplasma gondii in cell culture: an alternative to bioassay
Int J Parasitol. 2023 Dec 12:S0020-7519(23)00228-X. doi: 10.1016/j.ijpara.2023.12.002. Online ahead of print.ABSTRACTToxoplasma gondii is an apicomplexan protozoan parasite that can infect mammals and birds. The infection can cause acute toxoplasmosis and death in susceptible hosts. Bioassay using cats and mice has been the standard for the isolation of T. gondi from infected hosts for the past several decades. However, bioassay is labor-intensive, expensive, and involves using laboratory animals. To search alternative approaches and to work towards replacement of animal experiments, we summarized the key literature and co...
Source: International Journal for Parasitology - December 14, 2023 Category: Parasitology Authors: Tania Dawant Wei Wang Maria Spriggs Geraldo Magela de Faria Junior Laura Horton Nicole M Szafranski Helga Waap Pikka Jokelainen Richard W Gerhold Chunlei Su Source Type: research

Genome-based tools for onchocerciasis elimination: utility of the mitochondrial genome for delineating Onchocerca volvulus transmission zones
Int J Parasitol. 2023 Nov 20:S0020-7519(23)00207-2. doi: 10.1016/j.ijpara.2023.11.002. Online ahead of print.ABSTRACTNational programs in Africa have expanded their objectives from control of onchocerciasis (river blindness) as a public health problem to elimination of parasite transmission, motivated by the reduction of Onchocerca volvulus infection prevalence in many African meso- and hyperendemic areas due to mass drug administration of ivermectin (MDAi). Given the large, contiguous hypo-, meso-, and hyperendemic areas, sustainable elimination of onchocerciasis in sub-Saharan Africa requires delineation of geographic bo...
Source: International Journal for Parasitology - November 22, 2023 Category: Parasitology Authors: Katie E Crawford Shannon M Hedtke Stephen R Doyle Annette C Kuesel Samuel Armoo Mike Y Osei-Atweneboana Warwick N Grant Source Type: research

Genome-based tools for onchocerciasis elimination: utility of the mitochondrial genome for delineating Onchocerca volvulus transmission zones
Int J Parasitol. 2023 Nov 20:S0020-7519(23)00207-2. doi: 10.1016/j.ijpara.2023.11.002. Online ahead of print.ABSTRACTNational programs in Africa have expanded their objectives from control of onchocerciasis (river blindness) as a public health problem to elimination of parasite transmission, motivated by the reduction of Onchocerca volvulus infection prevalence in many African meso- and hyperendemic areas due to mass drug administration of ivermectin (MDAi). Given the large, contiguous hypo-, meso-, and hyperendemic areas, sustainable elimination of onchocerciasis in sub-Saharan Africa requires delineation of geographic bo...
Source: International Journal for Parasitology - November 22, 2023 Category: Parasitology Authors: Katie E Crawford Shannon M Hedtke Stephen R Doyle Annette C Kuesel Samuel Armoo Mike Y Osei-Atweneboana Warwick N Grant Source Type: research

Genome-based tools for onchocerciasis elimination: utility of the mitochondrial genome for delineating Onchocerca volvulus transmission zones
Int J Parasitol. 2023 Nov 20:S0020-7519(23)00207-2. doi: 10.1016/j.ijpara.2023.11.002. Online ahead of print.ABSTRACTNational programs in Africa have expanded their objectives from control of onchocerciasis (river blindness) as a public health problem to elimination of parasite transmission, motivated by the reduction of Onchocerca volvulus infection prevalence in many African meso- and hyperendemic areas due to mass drug administration of ivermectin (MDAi). Given the large, contiguous hypo-, meso-, and hyperendemic areas, sustainable elimination of onchocerciasis in sub-Saharan Africa requires delineation of geographic bo...
Source: International Journal for Parasitology - November 22, 2023 Category: Parasitology Authors: Katie E Crawford Shannon M Hedtke Stephen R Doyle Annette C Kuesel Samuel Armoo Mike Y Osei-Atweneboana Warwick N Grant Source Type: research

Genome-based tools for onchocerciasis elimination: utility of the mitochondrial genome for delineating Onchocerca volvulus transmission zones
Int J Parasitol. 2023 Nov 20:S0020-7519(23)00207-2. doi: 10.1016/j.ijpara.2023.11.002. Online ahead of print.ABSTRACTNational programs in Africa have expanded their objectives from control of onchocerciasis (river blindness) as a public health problem to elimination of parasite transmission, motivated by the reduction of Onchocerca volvulus infection prevalence in many African meso- and hyperendemic areas due to mass drug administration of ivermectin (MDAi). Given the large, contiguous hypo-, meso-, and hyperendemic areas, sustainable elimination of onchocerciasis in sub-Saharan Africa requires delineation of geographic bo...
Source: International Journal for Parasitology - November 22, 2023 Category: Parasitology Authors: Katie E Crawford Shannon M Hedtke Stephen R Doyle Annette C Kuesel Samuel Armoo Mike Y Osei-Atweneboana Warwick N Grant Source Type: research

Genome-based tools for onchocerciasis elimination: utility of the mitochondrial genome for delineating Onchocerca volvulus transmission zones
Int J Parasitol. 2023 Nov 20:S0020-7519(23)00207-2. doi: 10.1016/j.ijpara.2023.11.002. Online ahead of print.ABSTRACTNational programs in Africa have expanded their objectives from control of onchocerciasis (river blindness) as a public health problem to elimination of parasite transmission, motivated by the reduction of Onchocerca volvulus infection prevalence in many African meso- and hyperendemic areas due to mass drug administration of ivermectin (MDAi). Given the large, contiguous hypo-, meso-, and hyperendemic areas, sustainable elimination of onchocerciasis in sub-Saharan Africa requires delineation of geographic bo...
Source: International Journal for Parasitology - November 22, 2023 Category: Parasitology Authors: Katie E Crawford Shannon M Hedtke Stephen R Doyle Annette C Kuesel Samuel Armoo Mike Y Osei-Atweneboana Warwick N Grant Source Type: research

Genome-based tools for onchocerciasis elimination: utility of the mitochondrial genome for delineating Onchocerca volvulus transmission zones
Int J Parasitol. 2023 Nov 20:S0020-7519(23)00207-2. doi: 10.1016/j.ijpara.2023.11.002. Online ahead of print.ABSTRACTNational programs in Africa have expanded their objectives from control of onchocerciasis (river blindness) as a public health problem to elimination of parasite transmission, motivated by the reduction of Onchocerca volvulus infection prevalence in many African meso- and hyperendemic areas due to mass drug administration of ivermectin (MDAi). Given the large, contiguous hypo-, meso-, and hyperendemic areas, sustainable elimination of onchocerciasis in sub-Saharan Africa requires delineation of geographic bo...
Source: International Journal for Parasitology - November 22, 2023 Category: Parasitology Authors: Katie E Crawford Shannon M Hedtke Stephen R Doyle Annette C Kuesel Samuel Armoo Mike Y Osei-Atweneboana Warwick N Grant Source Type: research

Genome-based tools for onchocerciasis elimination: utility of the mitochondrial genome for delineating Onchocerca volvulus transmission zones
Int J Parasitol. 2023 Nov 20:S0020-7519(23)00207-2. doi: 10.1016/j.ijpara.2023.11.002. Online ahead of print.ABSTRACTNational programs in Africa have expanded their objectives from control of onchocerciasis (river blindness) as a public health problem to elimination of parasite transmission, motivated by the reduction of Onchocerca volvulus infection prevalence in many African meso- and hyperendemic areas due to mass drug administration of ivermectin (MDAi). Given the large, contiguous hypo-, meso-, and hyperendemic areas, sustainable elimination of onchocerciasis in sub-Saharan Africa requires delineation of geographic bo...
Source: International Journal for Parasitology - November 22, 2023 Category: Parasitology Authors: Katie E Crawford Shannon M Hedtke Stephen R Doyle Annette C Kuesel Samuel Armoo Mike Y Osei-Atweneboana Warwick N Grant Source Type: research

Genome-based tools for onchocerciasis elimination: utility of the mitochondrial genome for delineating Onchocerca volvulus transmission zones
Int J Parasitol. 2023 Nov 20:S0020-7519(23)00207-2. doi: 10.1016/j.ijpara.2023.11.002. Online ahead of print.ABSTRACTNational programs in Africa have expanded their objectives from control of onchocerciasis (river blindness) as a public health problem to elimination of parasite transmission, motivated by the reduction of Onchocerca volvulus infection prevalence in many African meso- and hyperendemic areas due to mass drug administration of ivermectin (MDAi). Given the large, contiguous hypo-, meso-, and hyperendemic areas, sustainable elimination of onchocerciasis in sub-Saharan Africa requires delineation of geographic bo...
Source: International Journal for Parasitology - November 22, 2023 Category: Parasitology Authors: Katie E Crawford Shannon M Hedtke Stephen R Doyle Annette C Kuesel Samuel Armoo Mike Y Osei-Atweneboana Warwick N Grant Source Type: research

Genome-based tools for onchocerciasis elimination: utility of the mitochondrial genome for delineating Onchocerca volvulus transmission zones
Int J Parasitol. 2023 Nov 20:S0020-7519(23)00207-2. doi: 10.1016/j.ijpara.2023.11.002. Online ahead of print.ABSTRACTNational programs in Africa have expanded their objectives from control of onchocerciasis (river blindness) as a public health problem to elimination of parasite transmission, motivated by the reduction of Onchocerca volvulus infection prevalence in many African meso- and hyperendemic areas due to mass drug administration of ivermectin (MDAi). Given the large, contiguous hypo-, meso-, and hyperendemic areas, sustainable elimination of onchocerciasis in sub-Saharan Africa requires delineation of geographic bo...
Source: International Journal for Parasitology - November 22, 2023 Category: Parasitology Authors: Katie E Crawford Shannon M Hedtke Stephen R Doyle Annette C Kuesel Samuel Armoo Mike Y Osei-Atweneboana Warwick N Grant Source Type: research

Genome-based tools for onchocerciasis elimination: utility of the mitochondrial genome for delineating Onchocerca volvulus transmission zones
Int J Parasitol. 2023 Nov 20:S0020-7519(23)00207-2. doi: 10.1016/j.ijpara.2023.11.002. Online ahead of print.ABSTRACTNational programs in Africa have expanded their objectives from control of onchocerciasis (river blindness) as a public health problem to elimination of parasite transmission, motivated by the reduction of Onchocerca volvulus infection prevalence in many African meso- and hyperendemic areas due to mass drug administration of ivermectin (MDAi). Given the large, contiguous hypo-, meso-, and hyperendemic areas, sustainable elimination of onchocerciasis in sub-Saharan Africa requires delineation of geographic bo...
Source: International Journal for Parasitology - November 22, 2023 Category: Parasitology Authors: Katie E Crawford Shannon M Hedtke Stephen R Doyle Annette C Kuesel Samuel Armoo Mike Y Osei-Atweneboana Warwick N Grant Source Type: research

Genome-based tools for onchocerciasis elimination: utility of the mitochondrial genome for delineating Onchocerca volvulus transmission zones
Int J Parasitol. 2023 Nov 20:S0020-7519(23)00207-2. doi: 10.1016/j.ijpara.2023.11.002. Online ahead of print.ABSTRACTNational programs in Africa have expanded their objectives from control of onchocerciasis (river blindness) as a public health problem to elimination of parasite transmission, motivated by the reduction of Onchocerca volvulus infection prevalence in many African meso- and hyperendemic areas due to mass drug administration of ivermectin (MDAi). Given the large, contiguous hypo-, meso-, and hyperendemic areas, sustainable elimination of onchocerciasis in sub-Saharan Africa requires delineation of geographic bo...
Source: International Journal for Parasitology - November 22, 2023 Category: Parasitology Authors: Katie E Crawford Shannon M Hedtke Stephen R Doyle Annette C Kuesel Samuel Armoo Mike Y Osei-Atweneboana Warwick N Grant Source Type: research

Genome-based tools for onchocerciasis elimination: utility of the mitochondrial genome for delineating Onchocerca volvulus transmission zones
Int J Parasitol. 2023 Nov 20:S0020-7519(23)00207-2. doi: 10.1016/j.ijpara.2023.11.002. Online ahead of print.ABSTRACTNational programs in Africa have expanded their objectives from control of onchocerciasis (river blindness) as a public health problem to elimination of parasite transmission, motivated by the reduction of Onchocerca volvulus infection prevalence in many African meso- and hyperendemic areas due to mass drug administration of ivermectin (MDAi). Given the large, contiguous hypo-, meso-, and hyperendemic areas, sustainable elimination of onchocerciasis in sub-Saharan Africa requires delineation of geographic bo...
Source: International Journal for Parasitology - November 22, 2023 Category: Parasitology Authors: Katie E Crawford Shannon M Hedtke Stephen R Doyle Annette C Kuesel Samuel Armoo Mike Y Osei-Atweneboana Warwick N Grant Source Type: research