The Effect of miR-98 and miR-214 on Apoptotic and Angiogenic Pathways in Hepatocellular Carcinoma HepG2 Cells
This study were conducted to explore the effect of these two miRNAs on some apoptotic and angiogenic genes namely, BCL-2, survivin, CCND1, CDC2, P53 and P21, VEGF, Hif-1 α, MMP-2, MMP-9, Ang-1, Ang-2, and FGF-1. miRNAs mimics and inhibitors transfection was used to investigate the role of both studied molecules in apoptosis and angiogenesis in HepG2 cells. QRT-PCR was used for Quantitative gene and miRNA expression analyses. The study revealed that miR-98 could ser ve as a pro-apoptotic factor through the upregulation of P53 gene expression levels. Besides, the anti-angiogenic effect of this miRNA was evident through...
Source: Indian Journal of Clinical Biochemistry - March 11, 2019 Category: Biochemistry Source Type: research

The Hemolyzed Sample: To Analyse Or Not To Analyse
AbstractPreanalytical errors constitute about 40 –65% of laboratory errors, of which 60% are due to hemolysis. This leads to imprecise reporting and misinterpretation of the actual concentration of analytes. Hence the aim of this study was to estimate the extent of different degrees of interference by visible hemolysis. 25 hemolysed sampl es along with their fresh unhemolysed sample were studied. Hemolyzed serum was mixed with unhemolyzed serum in predefined serial ratios from 100%, 70%, 50%, 30% and 10% to achieve different grades of hemolysis. Each dilution was analysed for BUN, creatinine, uric acid, phosphorus,...
Source: Indian Journal of Clinical Biochemistry - March 11, 2019 Category: Biochemistry Source Type: research

Assessment of Garcinia pedunculata Roxb. Role on Biological Markers in Carbon Tetra Chloride Induced Rat Model
AbstractGarcinia pedunculata Roxb. is an important medicinal plant of North Eastern (NE) region of India, having number of medicinal properties and used against various diseases in folk medicine. An empirical research was designed to carry out evaluation of hepatoprotective activity of the leaves ofG. pedunculata with special reference to its putative protective role. Methanolic extract from the dried leaf powder ofG. pedunculata Roxb. was prepared by hot continuous extraction method. The prepared extract was investigated at different dose levels for its hepatoprotective nature and further histopathological study was carri...
Source: Indian Journal of Clinical Biochemistry - March 6, 2019 Category: Biochemistry Source Type: research

In Vivo Validation of Folkloric Use of Costus pictus D. Don as Antidiabetic Plant in Assam, Using Streptozotocin Induced Wister Rat Model
AbstractCostus pictus D. Don belonging to the family Costaceae is one of the most commonly used plant among traditional healers in the upper Assam region in India, specifically used in the treatment of diabetes. Aerial parts of the plant are said to have potent anti diabetic property. The present study was aimed to evaluate the traditionally claimed antidiabetic activity of aerial parts ofC. pictus in animal models. Healthy male Wister rats (120  ± 30 gm) were used in the study and diabetes was induced by Streptozotocin (STZ) i.p. prepared by dissolving in citrate buffer (pH 4.5), along with nicotinami...
Source: Indian Journal of Clinical Biochemistry - March 6, 2019 Category: Biochemistry Source Type: research

A Study on Association Between Protein Carbonyl and Anti-cyclic Citrullinated Peptide Antibody in Rheumatoid Arthritis: Introducing a New Supplementary Biomarker
AbstractRedox state and immune mechanisms are two major factors implicated in rheumatoid arthritis (RA). Regarding some limitations of anti-cyclic citrullinated peptide (anti-CCP) antibody in RA diagnosis, recruiting another strong marker of oxidative stress could lead to more definitive diagnosis. To evaluate the potential of protein carbonyl content as a supplementary biomarker for RA. Eighty patients with RA attending the Research Center from 2015 to 2016 were recruited in this study. Smoker and alcoholic subjects, or those with any other systemic illness were excluded from the study. Demographic information and clinica...
Source: Indian Journal of Clinical Biochemistry - March 5, 2019 Category: Biochemistry Source Type: research

Evaluation of Relationship Between Arylesterase-Based Activity and Genetic Variants of Paraoxonase1 in T2DM Patients within Golestan Province
AbstractArylesterase activity of Paraoxonase-1 (PON1) enzyme may be play important role in initiation and progression of several diseases. Activity or serum level of Arylesterase can be affected by many genetic alterations such as SNPs. The reduction in the activity and serum level of Arylesterase could be involved in Type2 diabetes mellitus (T2DM). The aim of this investigation is to examine the association between Arylesterase activity and promoter polymorphism ( − 108C >  T) of PON1gene in patients with T2DM in Golestan Province, northern area of Iran. Achievement of this purpose was due to...
Source: Indian Journal of Clinical Biochemistry - March 5, 2019 Category: Biochemistry Source Type: research

Angiogenic Potential, Circulating Angiogenic Factors and Insulin Resistance in Subjects with Obesity
AbstractAltered vascular function and pathological angiogenesis are important factors common to the development of obesity and obesity-associated diseases. Most human studies relating obesity and angiogenesis have compared levels of angiogenic factors in obesity without looking at the serum angiogenic capacity which reflects the balance between the effects of angiogenic and angiostatic factors. Therefore, in this cross-sectional study, the serum angiogenic potential and levels of angiogenic factors in serum of obese (BMI  >  25 kg/m2) and lean subjects (BMI  
Source: Indian Journal of Clinical Biochemistry - February 22, 2019 Category: Biochemistry Source Type: research

Systematic Differences Between Total and Free Prostate-Specific Antigen Immunoassays: Comparison Using Passing and Bablok Regression
In this study we analyzedintermethod differences in total PSA (tPSA) and free PSA(fPSA) measurement using ARCHITECTi2000SR (Abbott Diagnostics) and COBAS E601 (Roche Diagnostics). A number of 160 blood samples were tested for tPSA and 50 samples for fPSA (selecting only sampleswith tPSA: 4.1 –10.0 μg/L). Passing–Bablok regression analysis was used to compare the two analytical methods fortPSA, fPSA and percentage of fPSA (%fPSA). A strong correlation was noticed between ARCHITECTi2000SR and COBAS E601 for tPSA, fPSA and %fPSA (r between 0.94 and 0.99). Concentrations of tPSA and fPSA measured by COBAS E...
Source: Indian Journal of Clinical Biochemistry - February 13, 2019 Category: Biochemistry Source Type: research

Induction of T7 Promoter at Higher Temperatures May Be Counterproductive
AbstractBacterial expression of recombinant proteins is the most popular and convenient method for obtaining large quantities of pure protein. The induction of T7 promoter with isopropyl- β-d-thiogalactopyranoside (IPTG) is widely used for expression of large quantities of proteins inEscherichia coli. It has been reported that basic T7 promoter is leaky and expresses cloned genes without induction. The effect of T7 promoter induction on expression of proteins at different temperature using flow cytometry has not yet been investigated. Green fluorescent protein (GFP) as a non-peptide tag can be used for protein solubil...
Source: Indian Journal of Clinical Biochemistry - February 8, 2019 Category: Biochemistry Source Type: research

p38 MAPK Inhibitor (SB203580) and Metformin Reduces Aortic Protein Carbonyl and Inflammation in Non-obese Type 2 Diabetic Rats
AbstractMicrovascular and macrovascular diseases are the main causes of morbidity in type 2 diabetes patients through chronic hyperglycaemic condition via oxidative stress and inflammation. Reactive oxygen species (ROS) activate p38 MAPK phosphorylation and inflammation which enhances protein modification by carbonylation. The use of metformin and a p38 MAPK inhibitor is hypothesised to reduce ROS production and inflammation but effects of metformin and p38 MAPK inhibitor (SB203580) on ROS production and inflammation in vascular type 2 diabetes mellitus non-obese (T2DM) have not been investigated. The Goto-Kakizaki rat T2D...
Source: Indian Journal of Clinical Biochemistry - January 31, 2019 Category: Biochemistry Source Type: research

Microalbuminuria in Chronic Obstructive Pulmonary Disease with and Without Co-morbidities
(Source: Indian Journal of Clinical Biochemistry)
Source: Indian Journal of Clinical Biochemistry - January 28, 2019 Category: Biochemistry Source Type: research

Single Cell Omics Approach: A Paradigm Shift in Diagnosis and Therapy of Cancer
(Source: Indian Journal of Clinical Biochemistry)
Source: Indian Journal of Clinical Biochemistry - January 10, 2019 Category: Biochemistry Source Type: research

Single Cell Omics of Breast Cancer: An Update on Characterization and Diagnosis
AbstractBreast cancer is recognized for its different clinical behaviors and patient outcomes, regardless of common histopathological features at diagnosis. The heterogeneity and dynamics of breast cancer undergoing clonal evolution produces cells with distinct degrees of drug resistance and metastatic potential. Presently, single cell analysis have made outstanding advancements, overshadowing the hurdles of heterogeneity linked with vast populations. The speedy progression in sequencing analysis now allow unbiased, high-output and high-resolution elucidation of the heterogeneity from individual cell within a population. C...
Source: Indian Journal of Clinical Biochemistry - January 8, 2019 Category: Biochemistry Source Type: research

Effects of Cornus mas Fruit Hydro-Methanolic Extract on Liver Antioxidants and Histopathologic Changes Induced by Cisplatin in Rats
AbstractCornelian cherry (Cornus mas) is a valuable source of phenolic antioxidants. The present study was aimed to investigate whetherCornus mas fruit hydro-methanolic extract (CMFE) can modulate the cisplatin-induced changes in liver antioxidant enzymes and histological structure. Forty Wistar rats were divided into a control group, cisplatin (Cis) group, CMFE group, CMFE 300  + Cis group, and the CMFE 700 + Cis group. After the intervention, blood and tissue samples were taken for biochemical and histopathological analysis. Cis caused reduction in the activity of liver antioxidant enzymes inc...
Source: Indian Journal of Clinical Biochemistry - January 5, 2019 Category: Biochemistry Source Type: research

Evaluation of Serum miRNA-24, miRNA-29a and miRNA-502-3p Expression in PCOS Subjects: Correlation with Biochemical Parameters Related to PCOS and Insulin Resistance
This study is focussed on the differential expression of miRNAs in serum of PCOS subjects compared to control and their correlation with metabolic and endocrine parameters. Anthropometry, hormone concentrations and biochemical characteristics were measured in healthy (n  = 20) and PCOS (n = 20) subjects. MiR-24, miR-29a and miR-502-3p were determined in serum by quantitative RT-PCR. The levels of miR-24 was significantly decreased in PCOS subjects (P  =  0.00) compared to control. No significant difference was observed in the levels of miR-29a and miR-502-3p in PCOS and control sub...
Source: Indian Journal of Clinical Biochemistry - January 3, 2019 Category: Biochemistry Source Type: research

Allium porrum and Bauhinia Variegata Mitigate Acute Liver Failure and Nephrotoxicity Induced by Thioacetamide in Male Rats
In conclusion, treatment withAllium porrum andBauhinia variegata extracts and their combination reduced deleterious effects of TAA on liver through antioxidant and anti-inflammatory properties. (Source: Indian Journal of Clinical Biochemistry)
Source: Indian Journal of Clinical Biochemistry - January 3, 2019 Category: Biochemistry Source Type: research

Intramuscular Followed by Oral Vitamin A Supplementation in Neonates with Birth Weight from 750 to 1250  g: A Randomized Controlled Trial
AbstractMultiple intramuscular (IM) injections of vitamin A have been shown to decrease bronchopulmonary dysplasia in very low birth weight (VLBW) neonates. However, this regime is neither practical nor popular. Oral vitamin A has failed to achieve adequate plasma levels. We aimed to investigate if a schedule of initial IM followed by oral supplementation can reduce vitamin A deficiency. This was a blinded, randomized controlled trial, conducted in a level III neonatal unit. Neonates with birth weight from 750 to 1250  g, were enrolled at the age of 24–96 h of life. They were randomly allocated to vitamin A...
Source: Indian Journal of Clinical Biochemistry - January 3, 2019 Category: Biochemistry Source Type: research

Polymorphisms of BIRC5 Gene is Associated with Chronic HBV Infection in Iranian Population
AbstractSurvivin can affect the progression of infection and is considered as a marker of various malignancies. The aim of the study was to investigate the possible association of gene polymorphisms of survivin (-1547A/G, -644C/T, -625 C/G, -241C/T, -31G/C, -141G/C) and chronic hepatitis B infection in Iranian patients. The genotypes of survivin SNPs were investigated by polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) method using 100 chronic HBV infected patients (HBV), 40 spontaneously recovered HBV subjects and 100 healthy controls (C). Serum level of survivin was determined using ELISA met...
Source: Indian Journal of Clinical Biochemistry - January 2, 2019 Category: Biochemistry Source Type: research

High Sensitivity C Reactive Protein: An Adjunct Diagnosis in Ruling Out Pediatric Tuberculosis
This study aims to determine the levels of high sensitivity CRP (hs CRP) among the pediatric tuberculosis cases. A case control study was conducted on 60 clinically diagnosed (clinical findings and radiography and/or contact history and/or Mantoux test) or microbiologically confirmed (smear and/or culture and/or Cartridge based Nucleic Acid Amplification test positive) pediatric tuberculosis cases  ≤ 12 years. hs CRP levels were estimated in the cases and healthy controls using ELISA. Median levels of serum hs CRP were significantly higher in pediatric tuberculosis cases (25 mg/l) as compared to ...
Source: Indian Journal of Clinical Biochemistry - January 1, 2019 Category: Biochemistry Source Type: research

Gene Editing in Clinical Practice: Where are We?
AbstractMultitude of gene-altering capabilities in combination with ease of design and low cost have all led to the adoption of the sophisticated and yet simple gene editing system that are clustered regularly interspaced short palindromic repeats (CRISPR)-associated protein 9 (CRISPR). The CRISPR/Cas9 system holds promise for the correction of deleterious mutations by taking advantage of the homology directed repair pathway and by supplying a correction template to the affected patient ’s cells. CRISPR is a tool that allows researchers to edit genes very precisely, easily and quickly. It does this by harnessing a me...
Source: Indian Journal of Clinical Biochemistry - January 1, 2019 Category: Biochemistry Source Type: research

Impact of Bisphenol A on Gonadotropic Hormone Levels in Children with Autism Spectrum Disorders
This study was done to evaluate association between the serum levels of hormones; follicle-stimulating (FSH), inhibin B (INHB), and estradiol (E2) and BPA in 49 ASD children compare d with 40 healthy control children. Serum levels of FSH, INHB, and E2 were lower in ASD group than that of control. Correlations between BPA and FSH, INHB, and E2 within autistic children were not significant. The observed results revealed that BPA may cause endocrine dysfunction in ASD children. (Source: Indian Journal of Clinical Biochemistry)
Source: Indian Journal of Clinical Biochemistry - December 10, 2018 Category: Biochemistry Source Type: research

Effects of Different Concentrations of Reversine on Plasticity of Mesenchymal Stem Cells
The objective of present study was to investigate the effect of different concentrations of reversine on the plasticity of ovine fetal bone-marrow mesenchymal stem cells (BM-MSCs). BM-MSCs were extracted from ovine fetal and cultured. Passaged-3 cells were evaluated for their differentiation potential into osteocytes and adipocytes cells. In the present study, BM-MSCs were culture plated in the presence of 0, 300, 600, 900 and 1200  nM of reversine. The number of viable cells was determined by MTT test after addition of different concentrations of reversine. Furthermore, expression of the nanog gene was evaluated. The...
Source: Indian Journal of Clinical Biochemistry - December 10, 2018 Category: Biochemistry Source Type: research

Association of Pseudohypoparathyroidism and Autoimmune Polyglandular Syndrome: Causal or Coincidental?
AbstractPseudohypoparathyroidism (PHP) is a state of parathyroid hormone resistance and is characterised by low serum calcium, and elevated serum phosphate and parathyroid hormone level. Association of PHP with autoimmune disorders is rare and seldom reported in the literature. Here we describe a case of PHP who subsequently developed multiple autoimmune disorders (type 3 polyglandular autoimmune syndrome), which has not been reported so far. (Source: Indian Journal of Clinical Biochemistry)
Source: Indian Journal of Clinical Biochemistry - December 10, 2018 Category: Biochemistry Source Type: research

Abstracts of 45th National Conference of Association of Clinical Biochemists of India (ACBICON 2018)
(Source: Indian Journal of Clinical Biochemistry)
Source: Indian Journal of Clinical Biochemistry - December 7, 2018 Category: Biochemistry Source Type: research

Evaluation of COMT Gene rs4680 Polymorphism as a Risk Factor for Endometrial Cancer
In conclusion, results of present meta-analysis showed no association between COMT Val158Met polymorphism and susceptibility to endometrial cancer. (Source: Indian Journal of Clinical Biochemistry)
Source: Indian Journal of Clinical Biochemistry - December 4, 2018 Category: Biochemistry Source Type: research

Blood Lead Levels in Occupationally Exposed Workers Involved in Battery Factories of Delhi-NCR Region: Effect on Vitamin D and Calcium Metabolism
AbstractWorkers involved in battery manufacturing or recycling factories are occupationally exposed to high concentrations of lead. In humans, lead can cause a wide range of biological effects depending upon the level and duration of exposure. The purpose of this study was to find out the blood lead levels (BLL) in occupationally exposed workers involved in battery industry in Delhi NCR region and to study whether lead affected the vitamin  D (vit D) and calcium metabolism. For this study 100 occupationally lead-exposed battery workers (LEBW) and 100 non-lead exposed controls (NLEC) were recruited. BLL were ...
Source: Indian Journal of Clinical Biochemistry - November 16, 2018 Category: Biochemistry Source Type: research

Blood Viscosity, Glycemic Markers and Blood Pressure: A Study in Middle-Aged Normotensive and Hypertensive Type 2 Diabetics
AbstractAltered blood viscosity (BV) may affect blood pressure (BP) and develops further complications in diabetes. A case-control study was performed to examine the relationship of erythrocyte sedimentation rate (ESR), hematocrit, fibrinogen, and BV with glycemic markers and BP in middle-aged normotensive and hypertensive type 2 diabetic patients and healthy controls. A total of 145 participants between age group 30 –50 years divided into three groups; controls (n = 60), type 2 diabetes mellitus (T2DM, n = 55), and T2DM with hypertension (T2DM + HTN, n = 3...
Source: Indian Journal of Clinical Biochemistry - November 1, 2018 Category: Biochemistry Source Type: research

Primary Antiphospholipid Syndrome Associated with Autoantibodies Against Golgi Bodies: A Case Report of a 32-Year-Old Female
We present the case report of a 32-year female whose complaints was abdominal pain for one  week and no history of previous foetal loss, who responded well to warfarin and has not developed systemic lupus erythematous even after follow up. Characteristic of this patient is the appearance of auto antibodies against Golgi bodies. Although antinuclear antibodies are seen in patients of rheu matic disease like systemic lupus erythematous, its presence in individuals with unusual presentation of antiphospholipid syndrome may facilitate in diagnosis. (Source: Indian Journal of Clinical Biochemistry)
Source: Indian Journal of Clinical Biochemistry - October 20, 2018 Category: Biochemistry Source Type: research

Differential Iron Status and Trafficking in Blood and Placenta of Anemic and Non-anemic Primigravida Supplemented with Daily and Weekly Iron Folic Acid Tablets
AbstractThe molecular mechanism of iron transfer across placenta in response to maternal anemic status/ iron supplementation is not clear. We hypothesized that maternal iron/ anemia status during early trimesters can be utilized as a biomarker tool to get estimates of placental iron status. Early interventions can be envisaged to maintain optimum placental/ foetal iron levels for healthy pregnancy outcomes. One hundred twenty primigravida were recruited and divided into non-anemic and anemic group on the basis of hemoglobin levels. The groups were randomly allocated to receive daily and weekly iron folic acid (IFA) tablets...
Source: Indian Journal of Clinical Biochemistry - October 5, 2018 Category: Biochemistry Source Type: research

Stem Cell Biology: A New Hope in Regenerations and Replenishments Therapy
(Source: Indian Journal of Clinical Biochemistry)
Source: Indian Journal of Clinical Biochemistry - October 1, 2018 Category: Biochemistry Source Type: research

Association of Vitamin D Receptor (FokI and BsmI) Gene Polymorphism with Bone Mineral Density and Their Effect on 25-Hydroxyvitamin D Level in North Indian Postmenopausal Women with Osteoporosis
AbstractOsteoporosis is a systemic disease with a strong genetic component. Vitamin D receptor (VDR) has been suggested as a candidate gene for osteoporosis. Therefore the present study was aimed to investigate the pattern of allelic variants of VDR gene polymorphism (FokI and BsmI), its influence on vitamin D levels and bone mineral density (BMD) in North Indian postmenopausal women with osteoporosis for possible genetic association. 254 postmenopausal osteoporotic women and 254 postmenopausal non osteoporotic women were included in the study. VDR FokI and BsmI gene polymorphism gene were assessed by the PCR-RFLP method. ...
Source: Indian Journal of Clinical Biochemistry - October 1, 2018 Category: Biochemistry Source Type: research

Neurochemical and Behavioral Dysfunctions in Pesticide Exposed Farm Workers: A Clinical Outcome
AbstractThe problem of pesticides is not new and its exposure to human due to indiscriminate use is largely associated with the health related problems including neurotoxicological alterations. High levels of pesticide residues and their metabolites in the dietary constituents, food materials, maternal blood, cord blood, placenta breast milk have been reported and linked to alterations in birth weight, crown heel length, head circumference, mid-arm circumference and ponderal index of the neonates. Epidemiological studies have suggested that exposure of pesticide to human could be a significant risk factor for neurological ...
Source: Indian Journal of Clinical Biochemistry - October 1, 2018 Category: Biochemistry Source Type: research

Monotonic Dose Effect of Bisphenol-A, an Estrogenic Endocrine Disruptor, on Estrogen Synthesis in Female Sprague-Dawley Rats
AbstractBisphenol-A (BPA) is a ubiquitous environmental chemical that produces adverse effect on reproduction system due to its potent estrogenic endocrine disruptive activity. The present study was aimed to investigate the monotonic dose effect of BPA on estrogen synthesis in female Sprague-Dawley rats. For this purpose, we administered three different doses of BPA (10, 50, 100  µg/kg bw/day) into rats and analyzed various biochemical, hormonal, molecular and histological parameters. 10 µg BPA treated rats showed significantly decreased levels of phase I detoxification agents (CYP450, Cyt-b5). Overex...
Source: Indian Journal of Clinical Biochemistry - October 1, 2018 Category: Biochemistry Source Type: research

Thiamine Deficiency Induced Dietary Disparity Promotes Oxidative Stress and Neurodegeneration
AbstractThiamine or vitamin B1 is a well known coenzyme and nutrient necessary for the assembly and right functioning of several enzymes involved in the energy metabolism. The present study evaluates oxidative stress and prevalence of neurodegenerative conditions in the brain following TD. The study was carried out on mice (Musmusculus) in three groups, namely control and thiamine-deficient group for 8 (TD 8) and 10 (TD 10) days. Lipid peroxidation was determined in terms of reduced glutathione (GSH) and thiobarbituric acid reactive substance (TBARS). The level of antioxidant enzymes such as catalase (CAT), glutathione red...
Source: Indian Journal of Clinical Biochemistry - October 1, 2018 Category: Biochemistry Source Type: research

Increased Nuclear Factor- κB/RelA Expression Levels in Human Colorectal Carcinoma in North Indian Patients
AbstractColorectal cancer is a major cause of cancer-related death in many countries. Inflammatory pathway is considered to play a major role in colorectal carcinogenesis. Nuclear factor kappa B (NF- κB) pathway is a link between inflammation and cancer. NF-κB is a transcription factor which belongs to the Rel family. Activation of NF-κB has been shown to play a role in cell proliferation, apoptosis, cytokine production, and oncogenesis. The aim of the present study was to evaluate the expres sion levels of NF-κB/RelA in colorectal carcinoma using Real-time PCR. For this study, tumor tissue was take...
Source: Indian Journal of Clinical Biochemistry - October 1, 2018 Category: Biochemistry Source Type: research

Citral, a Monoterpene Inhibits Adipogenesis Through Modulation of Adipogenic Transcription Factors in 3T3-L1 Cells
In this study, we investigated the inhibitory effect of citral against adipogenic genes in 3T3-L1 cells. The mouse fibroblast 3T3-L1 pre-adipocytes were differentiated into adipocytes using adipogenic cocktail (5  g/ml insulin, 0.5 mM isobutylmethylxanthine and 10 M dexamethasone). Differentiation of adipocytes was evaluated by assessing triglyceride accumulation assay and cell viability by MTT assay. The PI3K/AKT signaling, adipogenic specific transcription factors (PPARγ, SREBP-1c, FAS and CPD) and inf lammatory genes (TNF-α, IL-6 and MCP-1) were analyzed by western blotting and reverse transcr...
Source: Indian Journal of Clinical Biochemistry - October 1, 2018 Category: Biochemistry Source Type: research

Serum Biomarker Based Algorithms in Diagnosis of Ovarian Cancer: A Review
AbstractEpithelial ovarian cancer accounts for more than 90% of ovarian tumours and continues as a leading cause of death from gynaecological malignancies. It is often difficult to differentiate a benign ovarian mass from malignant ones. Invasive histopathological biopsy is used as the gold standard diagnostic tool to diagnose cancer in patients with ovarian mass. A wide spectrum of Biomarkers were tried in various studies to develop a non invasive diagnostic tool, out of which HE4 and CA 125 remain the only clinically useful biomarker. Consequently various Biomarker based algorithms i.e. Risk of Malignancy Index, risk of ...
Source: Indian Journal of Clinical Biochemistry - October 1, 2018 Category: Biochemistry Source Type: research

Cytochrome P450 Genes (CYP2E1 and CYP1A1) Variants and Susceptibility to Chronic Hepatitis B Virus Infection
AbstractHepatitis B virus (HBV) infection is a worldwide health concern which is associated with significant morbidity and mortality. Both viral and host factors have a significant effect on infection, replication and pathogenesis of HBV. The aim of this study was to investigate the effect ofCYP2E1 andCYP1A1 genetic variants on susceptibility to HBV. 143 individuals including 54 chronic HBV patients and 89 healthy controls were enrolled in the genotyping procedure. rs2031920 and rs3813867 atCYP2E1 as well as rs4646421 and rs2198843 atCYP1A1 loci were studied in all subjects using PCR –RFLP (restriction fragment lengt...
Source: Indian Journal of Clinical Biochemistry - October 1, 2018 Category: Biochemistry Source Type: research

Hypocalcemia in an 11  Year Old Child: A Difficult Case to Treat
We report a unique case of an eleven year old male child who presented with chronic kidney disease associated with severe hypocalcemia, tonic-clonic seizures, hypovitaminosis D but normal electroencephalogram and electrocardiography. The child required prolonged intravenous calcium gluconate therapy to correct his ionised calcium levels. (Source: Indian Journal of Clinical Biochemistry)
Source: Indian Journal of Clinical Biochemistry - October 1, 2018 Category: Biochemistry Source Type: research

Whether Lack of Measurement of Erythrocyte Cholinesterase or Acetyl Cholinesterase Activity Providing us Misleading Information in Organophosphorus Exposure?
(Source: Indian Journal of Clinical Biochemistry)
Source: Indian Journal of Clinical Biochemistry - October 1, 2018 Category: Biochemistry Source Type: research

Can High Titres of Anti Tissue Transglutaminase Antibodies Reduce the Need for Intestinal Biopsy for Diagnosis of Celiac Disease?
This study confirms that a small intestinal biopsy is not always necessary for the diagnosis of CD in symptomatic patients with high tTGA levels (>70  IU/ml). (Source: Indian Journal of Clinical Biochemistry)
Source: Indian Journal of Clinical Biochemistry - October 1, 2018 Category: Biochemistry Source Type: research

Stem Cell Biology: A New Hope in Regenerations and Replenishments Therapy
(Source: Indian Journal of Clinical Biochemistry)
Source: Indian Journal of Clinical Biochemistry - October 1, 2018 Category: Biochemistry Source Type: research

Hepcidin and Ferritin: Important Mediators in Inflammation Associated Anemia in Systemic Lupus Erythematosus Patients
AbstractSystemic Lupus Erythematosus is an autoimmune disease with female preponderance. Anemia is found in 50% of Systemic Lupus Erythematosus patients. This is a cross sectional case control study with 30 female Systemic Lupus Erythematosus patients having inflammation associated anemia (Hemoglobin  
Source: Indian Journal of Clinical Biochemistry - October 1, 2018 Category: Biochemistry Source Type: research

Hesperetin on Cell Surface Glycoconjugates Abnormalities and Immunohistochemical Staining with Cytokeratin in 7,12 Dimethylbenz(a)anthracene Induced Hamster Buccal Pouch Carcinogenesis
AbstractHesperetin, a naturally occurring citrus flavanone of the bioactive substance, possesses different pharmacological and biochemical activities including anti-cancer and anti-oxidants effect. The aim of the study to investigate that hesperetin on abnormalities of glycoconjugates (protein bound hexose, hexosamine, total sialic acid and fucose), histology (PAS staining) and immunoexpression of cytokeratin during 7,12-dimethylbenz(a)anthracene (DMBA) induced hamster buccal pouch (HBP) carcinogenesis. Oral tumors were developed in the buccal pouches of male golden Syrian hamsters by topical application of 0.5% DMBA thric...
Source: Indian Journal of Clinical Biochemistry - October 1, 2018 Category: Biochemistry Source Type: research

Ophthalmic Presentation of Disseminated Tuberculosis with Relapse-Immunological Profile
AbstractTB as the cause of uveitis varies from 0.5 to 10.5%; low sensitivity of confirmatory laboratory investigations and inconsistency of diagnostic criteria leads to paucity of data. Diagnosis requires a high level of suspicion and is often presumptive based on indirect evidences. Interferon gamma, Interleukin-2 and Neopterin are key biomarkers in immuno-regulation ofMycobacterium tuberculosis infection. The relative shift from Interleukin-2 towards Interferon gamma (Interferon gamma/Interleukin-2) is more discriminatory for active tuberculosis. Protein carbonyl and Malondialdehyde, as oxidative stress markers, characte...
Source: Indian Journal of Clinical Biochemistry - October 1, 2018 Category: Biochemistry Source Type: research

Diagnostic Utility of Cord Blood Thyroid Stimulating Hormone in Congenital Hypothyroidism in the Era of Expanded Newborn Screening
This study was to assess the diagnostic utility of cTSH  >  20 μIU/L for screening CH. Generation of new cTSH value was the main outcome measure, to increase specificity. Designed as a cross-sectional analytic study in the neonatal unit of teaching hospital, 1200 term neonates with birth weight ≥2500 g, with no perinatal complications were included. N ewborn cTSH assay was done by chemi-luminescence. All screen positive were followed up on day five, 14 and 28 of life, to rule in or out CH (true or false positive). Positive predictive value and specificity were calculated. Receiver operating cha...
Source: Indian Journal of Clinical Biochemistry - October 1, 2018 Category: Biochemistry Source Type: research

Correlation of Red Blood Cell Acetylcholinesterase Enzyme Activity with Various RBC Indices
AbstractCholinesterases belongs to class hydrolases. There are two types acetylcholinesterase and butyryl cholinesterase. Acetylcholinesterase present in nerve endings and also in the RBC membrane. It helps to maintain the shape and size of RBCs. Any change in shape and size of RBCs may affect the activity of Acetylcholinesterase. Thus this study aimed to estimate RBCs Acetylcholinesterase enzyme activity in various types of anemias and correlate the RBCs Acetylcholinesterase enzyme activity with various hematological indices such as Erythrocyte Sedimentation Rate (ESR), Mean Corpuscular Hemoglobin (MCH), Mean Corpuscular ...
Source: Indian Journal of Clinical Biochemistry - October 1, 2018 Category: Biochemistry Source Type: research

Investigation of Trace Elements in the Hair and Nail of Patients with Stomach Cancer
This study analyzed some trace elements level in hair and nail of patients with stomach cancer, and compared with their level in healthy controls. Trace elements (Cu, Fe, K, Li, Mg, Mn, Na, P, Se, Sr and Zn) are estimated in hair and nail of the 73 cancer patients and 83 controls by atomic absorption spectrophotometric method. The levels of Cu, K, Li, P and Se in hair and nail samples, were significantly higher in cases than controls. Levels of Mg and Sr were significantly lower in cases than controls. Fe level in hair samples was significantly higher in cases than controls. The mean concentrations of Fe, Se and P signific...
Source: Indian Journal of Clinical Biochemistry - October 1, 2018 Category: Biochemistry Source Type: research

Role of HAMP Genetic Variants on Pathophysiology of Iron Deficiency Anemia
AbstractHepcidin is a 25-amino acid peptide hormone produced by hepatocytes and plays a key role in body iron metabolism. Hepcidin deficiency is the cause of iron overload in hereditary hemochromatosis, iron-loading anemia, and its excess is associated with anemia of inflammation, chronic disease and iron deficiency anemia (IDA). The aims of this study was to evaluateHAMP gene mutation, namely IVS2  + 1(–G) (c.148–150 + 1del) and Gly71 Asp (c.212G >  A (rs104894696) association with iron status in IDA conditions. Our study participants were 500 IDA patients and 550 age and sex-m...
Source: Indian Journal of Clinical Biochemistry - October 1, 2018 Category: Biochemistry Source Type: research

3,3 ′-Diindolylmethane Encapsulated Chitosan Nanoparticles Accelerates Inflammatory Markers, ER/PR, Glycoprotein and Mast Cells Population During Chemical Carcinogen Induced Mammary Cancer in Rats
AbstractThe present study aimed to investigate the effect of 3,3 ′-diindolylmethane (DIM) on inflammatory markers, estrogen receptors (ER), progesterone receptors (PR), level of glycoprotein and the mast cell population in 7,12-dimethylbenz (a) anthracene (DMBA) 25 mg/kg b.wt. induced rat mammary carcinogenesis. After 8 weeks of tumor formation, rats had acce ss to an oral administrated with DIM 10 mg/kg b.wt. and DIM@CS-NP 0.5 mg/kg body weight respectively for 8 weeks. The oral administration of DIM@CS-NP 0.5 mg/kg b.wt. suppressed the Cox-2, NF-κB and TNF-α protein express...
Source: Indian Journal of Clinical Biochemistry - October 1, 2018 Category: Biochemistry Source Type: research