SHR0302 Improves Treg/Th17 Imbalance in Patients with Systemic Lupus Erythematosus
AbstractIL-6-mediated JAK1/STAT3 signaling pathway is involved in the development of Th17 cells, which play an essential role in the pathogenesis of various autoimmune diseases such as systemic lupus erythematosus (SLE). To evaluation of the regulatory and anti-inflammatory effects of the JAK1/STAT3 inhibition in SLE, we evaluated the effects of SHR0302 on regulatory T cell (Treg)/Th17 balance. Thirty-two patients with SLE and twenty-nine healthy subjects were enrolled in this study. The mRNA expression levels of anti- and pro-inflammatory cytokines, such as FOXP3, ROR- γt, IL-10, IL-17A, IL-21, and IRF-4, were determined...
Source: Indian Journal of Clinical Biochemistry - March 15, 2024 Category: Biochemistry Source Type: research

Co-existence of Congenital Hypothyroidism (CH) and TBG-Excess in a Boy Causing Simultaneous Elevation in Thyroid Stimulating Hormone (TSH) and Thyroxine (T4) Levels: First Report from India and Review of the Literature
This study highlights the importance of detailed biochemical and genetic testing for the differential diagnosis of thyroid disorders in patients exhibiting discrepant TFT results wh ich would avoid misdiagnosis and inappropriate treatment. (Source: Indian Journal of Clinical Biochemistry)
Source: Indian Journal of Clinical Biochemistry - March 15, 2024 Category: Biochemistry Source Type: research

Cancer Vaccines: A Novel Revolutionized Approach to Cancer Therapy
AbstractOver the past few decades, there has been significant advancement in the field of tumor immunotherapy. For many years vaccination against infectious diseases have been available. On the other hand very few cancer vaccines have been approved for human use. Ideal Cancer vaccines are biological response modifier work by stimulating both humoral and cellular immunity while overcoming the immunological suppression found in tumor. Two types of cancer vaccine: Prophylactic and therapeutic cancer vaccines are recommended for clinical use of individuals. HPV and HBV vaccines are the two widely used preventive vaccine used f...
Source: Indian Journal of Clinical Biochemistry - March 15, 2024 Category: Biochemistry Source Type: research

Utility of Urinary Biomarkers for Diagnosis of Acute Kidney Injury (AKI) in COVID-19
AbstractAcute kidney injury (AKI) is a complication of COVID-19 and urinary biomarkers of renal “stress” and “injury” can be utilised for an early diagnosis. In this prospective observational study, critically-ill adult patients with COVID-19 disease from November 2020 to May 2021 were recruited and urine samples collected at the time of admission to ICU, and subsequently at 12, 24 and 48 h and analysed for biomarkers of “renal injury” namely KIM-1, NGAL, IL-18, and cell cycle arrest biomarker IGFBP-7 and TIMP-2 which have been reported to increase following renal stress. Spot urine samples were collected and t...
Source: Indian Journal of Clinical Biochemistry - March 13, 2024 Category: Biochemistry Source Type: research

Exploring the Interplay Between Micronutrients and Cytokine Storm in Children with Multisystem Inflammatory Syndrome: ‘A Potential Mechanical Insight’
In conclusion, research on the connection between MIS-C and micronutrient deficiencies is still in its early stages. Although there is some evidence linking the two, additional research is required to determine a cause and effect. (Source: Indian Journal of Clinical Biochemistry)
Source: Indian Journal of Clinical Biochemistry - March 12, 2024 Category: Biochemistry Source Type: research

Point-of-Care Capillary Compared to Venous Bilirubin Measurement in Neonates
AbstractWe aimed to evaluate the agreement between the point-of-care (POC) capillary bilirubin measurement with POC venous samples and the reference laboratory measurement. We conducted a prospective comparative study, including neonates  ≥ 34 weeks of gestational age, and ≥ 72 h of age. The agreement between POC (Calmark Neo-Bilirubin, Sommargatan, Karlstad, Sweden) capillary, POC venous, and laboratory venous bilirubin was examined with the Bland–Altman plot and the Passing-Bablok regression analyses. The mean bili rubin was 13.54 (2.79) mg/dL in the POC capillary samples, 13.45 (2.69) mg/dL in the POC ve...
Source: Indian Journal of Clinical Biochemistry - March 10, 2024 Category: Biochemistry Source Type: research

Assessing Colorectal Cancer Susceptibility in Kashmir, India: Insights from Xenobiotic Metabolism Gene Variants and Family Cancer History —A Comprehensive Case–Control Study
AbstractA family history of cancer(FHC) is a known risk factor for CRC with both genetic and other factors. A case –control study was conducted to assess the association between FHC and CRC risk in Kashmir, India, with a detailed analysis of epidemiological data and information on multiple gene polymorphisms. We collected detailed information on FHC and a number of socio-demographic and lifestyle factors, and also obtained blood samples for genetic analysis from 246 histopathologically confirmed CRC cases and 246 individually matched controls. Conditional logistic regression models were used to calculate odds ratios (ORs...
Source: Indian Journal of Clinical Biochemistry - March 9, 2024 Category: Biochemistry Source Type: research

Lack of Glutamate Synthase in Streptococcus zooepidemicus makes a Natural Driving Force for Increasing Hyaluronic Acid Production
This study was undertaken to prove the dependence ofS. zooepidemicus life on Glutamate produced by Glutamine fructose 6-phosphate transaminase (GFAT) related to the HA pathway. The presence of Glutamate synthase (GOGAT) inS. zooepidemicus was investigated byin-silico, genome annotation, and enzymatic assay methods, respectively. Recombinant expression of GOGAT was investigated. The deletion of GFAT was done by the homologous recombination method. Recombinant expression of GOGAT and deletion of GFAT were performed simultaneously. In these last three steps, growth rate and HA production were determined. Not only the absence ...
Source: Indian Journal of Clinical Biochemistry - March 6, 2024 Category: Biochemistry Source Type: research

Exploring the Diagnostic Utility of Serum Cofilin-1 and 2 Levels in Patients with Acute Coronary Syndrome: A Case –Control Pilot Study
In conclusion, serum CFL-1 and 2 levels may not have greater significance in ACS diagnosis than traditional cardiac biomarkers. However, further cohort studies with a larger sample size must confirm the study’s findings. (Source: Indian Journal of Clinical Biochemistry)
Source: Indian Journal of Clinical Biochemistry - March 5, 2024 Category: Biochemistry Source Type: research

Predictive Value of Lymphocyte Based Indices to Determine Major Adverse Cardiovascular Events in Acute Coronary Syndrome Undergoing Percutaneous Coronary Intervention
AbstractAcute coronary syndrome (ACS) is leading cause of mortality worldwide. Percutaneous coronary intervention (PCI) is the main stay of treatment in patients presenting with ACS. However, studies have shown that PCI itself may induce and promote inflammatory process. Lymphocyte based hematological indices: neutrophil lymphocyte ratio (NLR), platelet lymphocyte ratio (PLR), monocyte lymphocyte ratio (MLR) and systemic inflammatory index (SII) have been shown as effective indicator of underlying inflammatory process. We conducted a prospective observational follow up study to observe the changes in NLR, PLR, MLR and SII ...
Source: Indian Journal of Clinical Biochemistry - March 2, 2024 Category: Biochemistry Source Type: research

Evaluation of Proflo-U ® Platform: A Novel Fluorescence-Based Point of Care Device for Quantitative Estimation of Urine Albumin
This study compares Proflo-U ® a novel PoC device that estimates urine albumin, with standard immunoturbidity based assays. Proflo-U® is a novel PoC device which works with an android based mobile application by Bluetooth connectivity. This unique fluorescence-based urine albumin assay has been compared with the well-establis hed gold standard Immunoturbidity based method on Beckman Coulter AU platform and also using Biosystem kit. The Proflo-U® generated data from standard recombinant human serum albumin (rHSA) and human urine spiked with rHSA has shown high correlationR2 ~ 0.99 andp >  0.05. This novel plat...
Source: Indian Journal of Clinical Biochemistry - February 25, 2024 Category: Biochemistry Source Type: research

Genetic Variants of AGO1*rs595961 and AGO2*rs4961280 with Susceptibility to Bladder Carcinoma
This study aimed to investigate the relationship betweenAGO1 and AGO2 gene variants with the elevated risk of bladder carcinoma (BLCA) using genetic, biochemical, and bioinformatic approaches. This retrospective study comprised of 252 participants [122 BLCA patients and 130 cancer-free controls], matched for age and gender. TheAGO1*rs595961 and AGO2*rs4961280 genetic variants were characterized using TaqMan genotyping assay. A bioinformatics framework, stratification analysis, and multivariate regression were employed. The genetic distribution ofAGO1*rs595961 was associated with an increased risk of bladder carcinoma under...
Source: Indian Journal of Clinical Biochemistry - February 24, 2024 Category: Biochemistry Source Type: research

Exploring the Role of Serum Lysyl Oxidase (LOX) Levels and LOX Gene Polymorphism (G473A) in Diabetic Nephropathy
AbstractDiabetic Nephropathy (DN) is a leading microvascular complication of diabetes with poor prognosis. Extra cellular matrix accumulation (ECM) leading to renal fibrosis is the key manifestation of diabetic kidney. Lysyl oxidase (LOX) plays an important role in cross linking of collagen. Altered LOX expression and LOX depended cross links with Diabetes have been documented both in rat and human model. Evidence of LOX gene polymorphism (G473A) has been shown limited to Diabetic foot ulcer. Studies on association of single nucleotide polymorphism (SNP) (G473A) of LOX gene with Diabetic nephropathy is scarce. A pilot stud...
Source: Indian Journal of Clinical Biochemistry - February 20, 2024 Category: Biochemistry Source Type: research

The First-Ever Investigation of SNP rs119461977 in SECISBP2/SBP2 Gene and its Implications for Hypothyroidism: A Novel Case –Control Research
This study indicates the novel association of rs119461977 of the SBP2 gene with hypothyroidism. This association underlines the importance of selenoproteins synthesis in the pathogenesis of hypothyroidism. (Source: Indian Journal of Clinical Biochemistry)
Source: Indian Journal of Clinical Biochemistry - February 20, 2024 Category: Biochemistry Source Type: research

Spirulina Platensis Alleviates γ- Radiation-Induced Brain Damage by Attenuating Oxidative Stress and Increasing Brain-Derived Neurotrophic Factor (BDNF) Levels in Male Albino Rats
AbstractIonizing radiation  can irreversibly damage the brain cells’ function and structure.Spirulina platensis (Sp) is widely employed as a dietary supplement because it has anti-inflammatory, antioxidant, and immunomodulating properties. Therefore, this work aims to investigate the protective properties of Sp towards γ-irradiation (IRR)-induced brain damage. The whole-body male albino rats were exposed to IRR with a 7 Gy single dose in the absence or presence of Sp (400 mg/kg body weight). The rats were divided into four groups: Group I (control), Group II (Sp), Group III (IRR), and Group IV (Sp + IRR +  Sp...
Source: Indian Journal of Clinical Biochemistry - February 17, 2024 Category: Biochemistry Source Type: research