TP53 rs1625895 is Related to Breast Cancer Incidence and Early Death in Iranian Population
In this study, we have investigated the role of rs1625895 polymorphism in breast cancer incidence in Iranian women. DNA extraction of 86 breast cancer patients and 96 control individuals have been used for allele-specific primer-PCR and genotyping of allele A and allele G of theTP53 rs1625895. Genotypes frequencies have been shown that GG homozygosis as the most frequent genotype is a significant association with increased risk of breast cancer development in Iranian women (odds ratio  = 6,p  =  0.002). On the other hand and in comparison to allele G, allele A could cause early death of breast c...
Source: Indian Journal of Clinical Biochemistry - June 21, 2018 Category: Biochemistry Source Type: research

β-Naphthyl Acetate in Acetone Produces a Dark Background for Staining of Esterase Activity on Gel
(Source: Indian Journal of Clinical Biochemistry)
Source: Indian Journal of Clinical Biochemistry - June 21, 2018 Category: Biochemistry Source Type: research

First Report of Association Between Rare α-Thalassemia Mutation ( HBA1 : c.298A & gt;T) and Hb Fontainebleau ( HBA2 : c.64G & gt;C)
Abstractα-Thalassemia is one of the most common monogenic diseases worldwide. The combination of alpha-chain variants with thalassemia mutations may lead to clinical and hematological characteristics, which is of importance for genetic counseling. The present study describes for the first time a rare α1-g lobin nonsense mutation, codon 99 (HBA1: c.298A>T) associated with a α2-chain variant Hb Fontainebleau (HBA2: c.64G>C) in a family from northern Iran. The case is a 23-year-old man with hypochromic microcytic anemia that requested for prenatal diagnosis. The combination of α1-globin mutation ...
Source: Indian Journal of Clinical Biochemistry - June 18, 2018 Category: Biochemistry Source Type: research

Comparison of Serum Thyroglobulin Levels in Differentiated Thyroid Cancer Patients Using In-House Developed Radioimmunoassay and Immunoradiometric Procedures
AbstractThyroglobulin (Tg) is a proven tumor marker in the follow-up and post-operative management of patients with differentiated thyroid cancer (DTC). All assays for serum thyroglobulin (s-Tg) are based on immunoassays, however, the assay technique has a bearing on the variations seen in the estimations. We studied this using four in-house developed radioimmunoassays (RIA) and immunoradiometric assays (IRMA). Limit of detection, working range, recovery, dilution test, precision profiles and method comparison were evaluated. All four methods were used for the estimation of s-Tg in DTC patients and also compared for their ...
Source: Indian Journal of Clinical Biochemistry - June 18, 2018 Category: Biochemistry Source Type: research

Heterophile Antibody to Adrenocorticotropin Hormone Interfering with the Investigation of Cushing ’s Syndrome
AbstractThe practice of medicine depends on the accuracy of biochemical assays. The high prevalence of incidental masses on imaging necessitates a correct biochemical diagnosis before proceeding to radiological studies. Hormonal assays, tumour markers, and markers of cardiac injury are particularly susceptible to heterophile antibody interference which may lead to inaccurate and misleading results, inappropriate investigation and/or treatment, patient concern and potential harm. A case of heterophile antibody interference in the measurement of ACTH in a patient with Cushing ’s syndrome resulting in unnecessary invasi...
Source: Indian Journal of Clinical Biochemistry - June 16, 2018 Category: Biochemistry Source Type: research

Evaluation of the Expression Level and Hormone Receptor Association of miR-126 in Breast Cancer
AbstractBreast cancer is a major cause of cancer-related death in women worldwide. miRNAs are new players of breast tumorigenesis, used as diagnostic and prognostic biomarkers. Among various miRNAs, miR-126 has been proposed to have a tumor suppressive role in HER2 positive cancer. However, to have a better understanding of its role, further validation is required. The aim of this study was evaluating miR-126 expression level in breast cancer tissues and investigating its potential association with HER2, estrogen and progesterone receptors. miR-126 expression level was measured in 108 specimens including 78 malignant and 3...
Source: Indian Journal of Clinical Biochemistry - June 16, 2018 Category: Biochemistry Source Type: research

Chrysin Pretreatment Improves Angiotensin System, cGMP Concentration in L-NAME Induced Hypertensive Rats
The objective of the present study was to investigate the effects of chrysin with flavnoids, on L-NAME-induced hypertensive rats. Methods: An experimental hypertensive animal (180 –220 g) model was induced by L-NAME intake on rats. In treatment chrysin was orally administered 25 mg/kg body weight (b.w.). Blood pressure was measured by tail cuff plethysmography system. Cardiac and vascular function was evaluated by Langendorff isolated heart system with Angiotensin II (Ang -II), Hexo oxygenase (HO-1), cyclic guanosine monophosphate (cGMP) concentration in tissues respectively. Rats with hypertension showed a...
Source: Indian Journal of Clinical Biochemistry - June 14, 2018 Category: Biochemistry Source Type: research

Acyclic Isoprenoid Attenuates Lipid Anomalies and Inflammatory Changes in Hypercholesterolemic Rats
Abstract The present study was aimed to explore the antihyperlipidemic and anti-inflammatory effect of acyclic isoprenoid on high fat diet fed rats. Hypercholesterolemia was induced by the diet comprising of the normal rat chow 84.3%, lard 5%, yolk powder 10%, cholesterol 0.2% and 0.5% bile salt were fed to the rats for the period of 8  weeks. The results showed that abnormally elevated levels of plasma lipid profiles. Three different doses of acyclic isoprenoid (20, 40 and 80 mg/kg b.w/day) were administered orally to hypercholesterolemia suffering rats for the period of 30 days. Among these three doses o...
Source: Indian Journal of Clinical Biochemistry - June 13, 2018 Category: Biochemistry Source Type: research

Cerium Oxide Nanoparticle Effects on Paraoxonase-1 Activity and Oxidative Toxic Stress Induced by Malathion: A Potential Antioxidant Compound, Yes or No?
AbstractCerium oxide nanoparticles (CeNPs) are one of the most widely used and important nanoparticles in addition to possessing strong antioxidative properties and inhibiting free radicals. Paraoxonase-1 (PON1) is one of the enzymes that protect the body against damage caused by oxidative stress. The purpose of this study was to investigate the effect of CeNPs on the activity of PON1 as well as biomarkers of oxidative stress in the toxicity of malathion. 48 Albino Wistar male rats with weight range of 180 –250 g were randomly divided into 8 groups, Group 1: healthy control, injection of normal saline, Group 2: ...
Source: Indian Journal of Clinical Biochemistry - June 6, 2018 Category: Biochemistry Source Type: research

Effect of Severity of Opiate Use on Cardiometabolic Profile of Chronic Opiate Dependents of Western Rajasthan
AbstractLack of cardiometabolic profile data based on severity of opiate dependence for opiate abusers. The study aimed to evaluate the effect severity of opiate abuse on the cardiometabolic profile of male opiate abusers without co-morbidities. The study included 30 healthy controls (HCs), 90 prospective chronic opiate (opium and heroin) abusers, with and without co-dependence of smoking and tobacco-chewing. The subjects were categorized based on severity of opiate dependence questionnaire (SODQ) and metabolic syndrome (MS) based on NCEP ATP-III criteria and fasting blood samples analyzed for sugar, insulin, insulin resis...
Source: Indian Journal of Clinical Biochemistry - June 6, 2018 Category: Biochemistry Source Type: research

Modulatory Effects of Berberine Chloride on Lipid Profile, Oxidant Status and Insulin Signaling Molecules in Streptozotocin Induced Diabetic Rats
AbstractThe goal of the present study was to evaluate the effect of Berberine chloride (BC) on lipid profile, oxidant status and insulin signaling molecules in Streptozotocin (STZ) induced diabetic rat model. Diabetes was induced in rats by a single dose of intraperitoneal administration of STZ (40  mg/kg b.w). Diabetic rats were treated with BC (50 mg/kg b.w) and glibenclamide (6 mg/kg b.w) for 45 days. BC treated diabetic rats showed significant (p  
Source: Indian Journal of Clinical Biochemistry - May 21, 2018 Category: Biochemistry Source Type: research

Alkaline Phosphatase Activity of Normal and Malignant Human Lymphocytes
AbstractBone marrow aspirates form patients with multiple myeloma were obtained and lymphocytes were isolated using density gradient centrifugation. The APase activity of the samples ranged from 12 to 88  nmol/0.2 × 106 cells as compared to the very low activity present in normal human peripheral blood lymphocytes PBL. Neither the unstimulated nor the mitogen stimulated lymphocytes from peripheral blood of normal humans showed appreciable APase activity. The lymphocytes from bone marrow of multiple myeloma patients as well as myeloma cell lines —RPMI 8226 and U266 B1 express APase activity co...
Source: Indian Journal of Clinical Biochemistry - May 9, 2018 Category: Biochemistry Source Type: research

Non-HDL as a Valid Surrogate Marker of Small Dense LDL in a Young Indian Population
This study aimed at identifying non-HDL as a superior surrogate marker of sdLDL cholesterol in a young Indian population. 161 healthy subjects  
Source: Indian Journal of Clinical Biochemistry - May 8, 2018 Category: Biochemistry Source Type: research

Prevalence of Nutritional Anemia and Hyperhomocysteinemia in Urban Elderly
AbstractThe data on the prevalence of nutritional anemia among the urban elderly population in India was limited. Hence, the present study was carried out with an aim to assess the prevalence of nutritional anemia and its association with vitamin B12, folate, ferritin and homocystine among the urban elderly population. A community- based cross-sectional study was carried out among 282 urban elderly ( ≥ 60 years) subjects (186 males and 96 females) in Hyderabad. Fasting blood samples were collected and hemoglobin (Hb) was estimated by cyanmethemoglobin method. Plasma Folic acid and vitamin B12 levels were est...
Source: Indian Journal of Clinical Biochemistry - May 4, 2018 Category: Biochemistry Source Type: research

Haemoglobin A1c or Glycated Albumin for Diagnosis and Monitoring Diabetes: An African Perspective
AbstractDiabetes mellitus (DM) has reached epidemic proportions across the globe with the largest increases seen in sub-Saharan Africa. Those that are diagnosed are largely poorly controlled. This review summarizes the limitations of the use of glycated haemoglobin (HBA1c) in Africa and current knowledge on the utility of glycated albumin and fructosamine in African patients. The diagnosis and monitoring of DM in African patients may be compromised by associated conditions like sickle cell anaemia, chronic kidney disease and HIV infection. Glycated albumin reflects short term glycaemia and is not affected by many condition...
Source: Indian Journal of Clinical Biochemistry - May 3, 2018 Category: Biochemistry Source Type: research

Evaluation of Circulating Plasma VEGF-A, ET-1 and Magnesium Levels as the Predictive Markers for Proliferative Diabetic Retinopathy
In this study, we included thirty subjects with T2DM in each of the three groups  namely, T2DM cases without retinopathy, Non Proliferative DR (NPDR) and Proliferative DR (PDR) retinopathy. The glycemic status, circulating plasma VEGF-A, ET-1 levels, serum magnesium and lipids were estimated and compared among the groups. An ROC was drawn to evaluate VEGF-A, ET-1 and serum magn esium levels as the predictive markers for PDR. On comparison VEGF-A, ET-1 and serum magnesium levels showed a significant difference among the three groups. PDR cases had higher circulating levels of VEGF-A, ET-1 and low serum magnesium levels...
Source: Indian Journal of Clinical Biochemistry - May 3, 2018 Category: Biochemistry Source Type: research

Tau Protein Levels in ADHD
(Source: Indian Journal of Clinical Biochemistry)
Source: Indian Journal of Clinical Biochemistry - April 19, 2018 Category: Biochemistry Source Type: research

Role of Maternal Serum Human Placental Lactogen in First Trimester Screening
The objectives of this study was to measure and correlate maternal serum hPL with free β hCG, maternal age, maternal age related risk ratio and calculated risk ratio of first trimester screen ing. After obtaining permission from the Institutional Ethics Committee, hPL and free β hCG were measured from the serum of 84 pregnant women aged 20–40 years in 11–13th weeks + 6 days of gestation who underwent dual test during their antenatal check-up. Independent t test, Pearson’s co rrelation, Spearman’s correlation, Mann–Whitney U test, ANOVA were used wherever appro...
Source: Indian Journal of Clinical Biochemistry - April 19, 2018 Category: Biochemistry Source Type: research

Psidium guajava Leaf Extracts and Their Quercetin Protect HepG2 Cell Lines Against CCL 4 Induced Cytotoxicity
AbstractThe present study was carried out to evaluate the in vitro cytoprotective effects ofPsidium guajava and their isolated quercetin fraction to reduce the CCl4 (carbon tetrachloride) induced toxicity in HepG2 cell lines (Hepatocellular carcinoma G2). Silymarin was used as a standard drug to compare the protective effects of plant extracts in infected cell lines. MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl-tetrazolium bromide) assay, cell viability assay, leakage parameters [Aspartate aminotransferase (AST), Alanine aminotransferase (ALT) and Lactate dehydrogenase (LDH)], lipid peroxidation and reduced glutathione (...
Source: Indian Journal of Clinical Biochemistry - April 11, 2018 Category: Biochemistry Source Type: research

miR-581-Related Single Nucleotide Polymorphism, rs2641726, Located in MUC4 Gene, is Associated with Gastric Cancer Incidence
This study demonstrated that rs2641726 C allele was significantly associated with the incidence of gastric cancer, odds ratio = 3.382, 95% confidence interval: 1.840–6.217 (P 
Source: Indian Journal of Clinical Biochemistry - April 10, 2018 Category: Biochemistry Source Type: research

Correlation Between Intron 4 Polymorphism of the Endothelial Nitric Oxide Synthase Gene and Cardiovascular Risk with the Numbers of Circulating Endothelial Progenitor Cells in Healthy Subjects
AbstractIn the present study, we investigated the relationship between an important 27  bp repeat polymorphism in intron 4 of eNOS and numbers of circulating EPCs in presence of cardiovascular disease (CVD) risk factors in a group of healthy human volunteers. The study comprised of 45 healthy subjects (30–50 years). These subjects had various degrees of CVD risk but no history of CVD. The repeat polymorphism of eNOS was detected by polymerase chain reaction and EPC levels were analyzed by flow cytometry. We observed a good association between the intronic 4 mutant a/b genotype and the combined Framingham ri...
Source: Indian Journal of Clinical Biochemistry - April 1, 2018 Category: Biochemistry Source Type: research

Role of Brain Biomarker in Predicting Clinical Outcome in Hypertensive Cerebrovascular Ischemic Stroke
This study was undertaken to evaluate the role of serum neuron specific enolase (NSE) in prediction of disability and neurological worsening in hypertensive ischemic cerebrovascular stroke. 80 hypertensive ischemic stroke patients diagnosed by a neurologist as per WHO definition along with radiological findings suggestive of cerebrovascular stroke and differentiating from hemorrhagic stroke and 60 controls having essential hypertension coming to hospital because of regular checkup or headache but with no neurological disease were included in the study. Neurological disability was assessed by NIHSS at the time of admission ...
Source: Indian Journal of Clinical Biochemistry - April 1, 2018 Category: Biochemistry Source Type: research

Urinary Urea, Uric Acid and Hippuric Acid as Potential Biomarkers in Multiple Sclerosis Patients
The objective was to investigate the level of some urinary metabolites (urea, uric acid and hippuric acid) in patients with MS and correlate their levels to the severity of the disease, MS subtypes and MS treatment. The urine samples were collected from 73 MS patients-48 with RRMS and 25 with SPMS- and age matched 75 healthy controls. The values of urinary urea, uric acid and hippuric acid in MS patients were significantly decreased, and these metabolites in SPMS pattern showed significantly decrease than RRMS pattern. Also showed significant inverse correlation with expanded disability status scale and number of relapses....
Source: Indian Journal of Clinical Biochemistry - April 1, 2018 Category: Biochemistry Source Type: research

Effects of Alternanthera sessilis on Liver Function in Carbon Tetra Chloride Induced Hepatotoxicity in Wister Rat Model
AbstractAlternanthera sessilis commonly known as ‘sessile joy weed’ or ‘dwarf copperleaf’ is found throughout the hotter parts of India up to an altitude of 1200 m2. In Assam, the plant has been traditionally used in the treatment of jaundice along with other ailments. The study focuses primarily on the evaluation of hepatoprotective activity of the plant with special references to its putative protective role in carbon tetrachloride induced liver injury on Wister albino rat. The in vivo hepatoprotective activity of the methanolic extract at the dose of 250  mg/kg body weight was highly ef...
Source: Indian Journal of Clinical Biochemistry - April 1, 2018 Category: Biochemistry Source Type: research

Linear Analysis of Autonomic Activity and Its Correlation with Creatine Kinase-MB in Overt Thyroid Dysfunctions
This study was designed to investigate the correlation of CK and its isoform CK-MB with thyroid profile and linear parameters of heart rate variability (HRV). The study was conducted on 35 hypothyroid and hyperthyroid patients each, and 25 age-matched healthy controls. Autonomic activity was assessed by simple short term 5-min HRV. Biochemical evaluation of serum thyroid profile, CK-NAC and CK-MB were estimated in all the subjects. Our results demonstrated low HRV in hypo- as well as hyperthyroid patients. We observed significantly higher serum CK levels in hypothyroid patients when compared to hyperthyroids and controls. ...
Source: Indian Journal of Clinical Biochemistry - April 1, 2018 Category: Biochemistry Source Type: research

Protective Effect of Allyl Isothiocyanate on Glycoprotein Components in 7,12-dimethylbenz(a)anthracene Induced Mammary Carcinoma in Rats
AbstractThe present study aimed to investigate the protective effect of allyl isothiocyanate (AITC) on glycoprotein components in 7,12-dimethylbenz(a)anthracene (DMBA) induced mammary carcinogenesis in female Sprague –Dawley rats. Mammary tumor was induced by a single dose of DMBA (25 mg/rat) injected subcutaneously near mammary gland. The levels of glycoprotein components such as hexose, hexosamine and sialic acid were analyzed colorimetrically in plasma, mammary and liver tissues. We observed an increased l evels of glycoprotein components in plasma, mammary and liver tissues in cancer bearing rats. It was fur...
Source: Indian Journal of Clinical Biochemistry - April 1, 2018 Category: Biochemistry Source Type: research

Genetic Polymorphism of CYP2C9 Among Sistani Ethnic Group in Gorgan
This study included 140 Sistani, referred to the health center of Gorgan. CYP2C9 genotyping was carried out by polymerase chain reaction –restriction fragment length polymorphism technique. The allele frequency of CYP2C9*1, CYP2C9*2 and CYP2C9*3 was 76.1, 16.1 and 7.8%, respectively. The frequency of CYP2C9*1/*1, CYP2C9*1/*2, CYP2C9*1/*3, CYP2C9*2/*2, CYP2C9*2/*3 and CYP2C9*3/*3 genotypes was 53.9, 22.1, 11.4, 2.9, 4.3% and nil, re spectively. In this study the genotypic variations of the CYP2C9 allele among the Sistani ethnic group was investigated and great differences were observed in comparison to other populatio...
Source: Indian Journal of Clinical Biochemistry - April 1, 2018 Category: Biochemistry Source Type: research

Plasma Neutrophil Elastase, α 1 -Antitrypsin, α 2 -Macroglobulin and Neutrophil Elastase–α 1 -Antitrypsin Complex Levels in patients with Dengue Fever
In conclusion, significantly elevated NE and reduced inhibitors level in dengue fever indicate these parameters could be of significance in DF particularly for the assessment of progression of inflammatory processes. (Source: Indian Journal of Clinical Biochemistry)
Source: Indian Journal of Clinical Biochemistry - April 1, 2018 Category: Biochemistry Source Type: research

Measured Versus Calculated Small Dense LDL-Cholesterol and Cardiometabolic Traits in a South African Population
AbstractSmall-dense low density lipoprotein (sdLDL) is increasingly viewed as a marker for evaluating atherogenic risk, however its clinical uptake is hampered by the cumbersomeness of available methods. Consequently, a number of alternative methods for the estimation of sdLDL have been developed and none have been tested in a population from Africa. We evaluated an equation to estimate sdLDL-C from classic lipid parameters in South Africans. This is a cross-sectional study involving 1550 participants in which direct measurement of sdLDL in 237 participants was performed using a homogeneous enzymatic assay. Their mean age ...
Source: Indian Journal of Clinical Biochemistry - March 22, 2018 Category: Biochemistry Source Type: research

Irisin and Visfatin Predicts Severity of Diabetic Nephropathy
AbstractAlthough the roles of irisin and visfatin have been well established in diabetes mellitus, there are limited studies about their association in diabetic nephropathy. The present study was designed to assess the levels of irisin and visfatin and their association with the severity of diabetic nephropathy. 43 diabetic nephropathy cases and 43 diabetic subjects without nephropathy were enrolled in the study. Serum levels of irisin and visfatin were compared in both the groups. Irisin and visfatin were significantly increased in diabetic nephropathy cases when compared with diabetes subjects without nephropathy. eGFR w...
Source: Indian Journal of Clinical Biochemistry - March 21, 2018 Category: Biochemistry Source Type: research

Effect of Atorvastatin on Serum Para-Oxonase-1 and C-Reactive Protein in Wistar Rats
AbstractStatins have been widely used in the treatment of hypercholesterolemia and atherosclerotic disease. Atherosclerosis is an ongoing inflammatory response which is involved in mediating all stages of this multifactorial disease. The present study focuses on the long term effect of atorvastatin on the anti-atherogenic and anti-inflammatory properties with reference to para-oxonase and C-reactive protein levels in rats. Thirty six Wistar albino rats obtained from the central animal house were divided into 6 groups with 6 rats in each group. Group I and IV served as the control for male and female rats respectively. Grou...
Source: Indian Journal of Clinical Biochemistry - March 17, 2018 Category: Biochemistry Source Type: research

Plasma Hydrogen Peroxide: A Myth or Reality?
(Source: Indian Journal of Clinical Biochemistry)
Source: Indian Journal of Clinical Biochemistry - March 12, 2018 Category: Biochemistry Source Type: research

Tissue-Resident Memory Cells: New Marked Shield to Fight Cancers
(Source: Indian Journal of Clinical Biochemistry)
Source: Indian Journal of Clinical Biochemistry - March 7, 2018 Category: Biochemistry Source Type: research

Hypocalcemia in an 11  Year Old Child: A Difficult Case to Treat
We report a unique case of an eleven year old male child who presented with chronic kidney disease associated with severe hypocalcemia, tonic-clonic seizures, hypovitaminosis D but normal electroencephalogram and electrocardiography. The child required prolonged intravenous calcium gluconate therapy to correct his ionised calcium levels. (Source: Indian Journal of Clinical Biochemistry)
Source: Indian Journal of Clinical Biochemistry - March 3, 2018 Category: Biochemistry Source Type: research

Caffeic Acid Modulates miR-636 Expression in Diabetic Nephropathy Rats
AbstractWe investigated the action of caffeic acid in regulating miR-636 expression level in kidney of streptozotocin-induced diabetic rats. Streptozotocin-induced diabetic rats were orally treated with caffeic acid at 40  mg/kg/day for 8 weeks. At the end of the treatment, body and kidney weight and blood glucose levels were determined, blood, urine, and kidneys were collected for biochemical and histological examination. Expression levels of miR-636 were determined in liver by qRT-PCR. Induction of diabetic nephr opathy by streptozotocin was evidenced by displayed elevated levels of serum creatinine, blood urea...
Source: Indian Journal of Clinical Biochemistry - February 26, 2018 Category: Biochemistry Source Type: research

Dual Effect of IL-6 -174 G/C Polymorphism and Promoter Methylation in the Risk of Coronary Artery Disease Among South Indians
In conclusion, the promoter polymorphism -174 G/C is associated with CAD risk and further carriers of ‘C’ allele at -174 locus showed significant hypo methylation which could contribute to increased risk of CAD. The present study highlights the association of allele and genotypes with differential DNA methylation of CpG islands in the IL-6 promoter region which may affect IL-6 gene regulation. (Source: Indian Journal of Clinical Biochemistry)
Source: Indian Journal of Clinical Biochemistry - February 21, 2018 Category: Biochemistry Source Type: research

Ophthalmic Presentation of Disseminated Tuberculosis with Relapse-Immunological Profile
AbstractTB as the cause of uveitis varies from 0.5 to 10.5%; low sensitivity of confirmatory laboratory investigations and inconsistency of diagnostic criteria leads to paucity of data. Diagnosis requires a high level of suspicion and is often presumptive based on indirect evidences. Interferon gamma, Interleukin-2 and Neopterin are key biomarkers in immuno-regulation ofMycobacterium tuberculosis infection. The relative shift from Interleukin-2 towards Interferon gamma (Interferon gamma/Interleukin-2) is more discriminatory for active tuberculosis. Protein carbonyl and Malondialdehyde, as oxidative stress markers, characte...
Source: Indian Journal of Clinical Biochemistry - February 21, 2018 Category: Biochemistry Source Type: research

Hepatic and Renal Biochemical Markers as Predictors of Mortality Among Critically Ill Systemic Inflammatory Response Syndrome Patients
AbstractSystemic inflammatory response syndrome (SIRS) is a frequently encountered complication seen in intensive care unit patients and remains a common cause of mortality. Assessing prognosis of those becomes a priority and indeed we have various efficient scoring systems for the same. However they use enormous data and involve complex calculations for scoring. We intended to find a simple, inexpensive, accurate diagnostic tool of certain markers to predict mortality outcome among critically ill SIRS patients and to evaluate their efficiency in comparison to Acute Physiology and Chronic Health Evaluation II (APACHE II) s...
Source: Indian Journal of Clinical Biochemistry - February 21, 2018 Category: Biochemistry Source Type: research

The Effects of Firsthand and Secondhand Cigarette Smoking on Immune System Cells and Antibodies in Saudi Arabian Males
AbstractFirsthand and, to a lesser degree, secondhand tobacco smoking are considered the greatest causes of preventable illnesses and premature death worldwide. Firsthand and secondhand smoking have adverse consequences on the immune system, although these effects are not fully understood. A few serological studies have been done on firsthand and secondhand smokers in Saudi Arabia. The present study investigates the effects of firsthand and secondhand smoking on the immune system of randomly chosen male firsthand (50 subjects) and secondhand (50 subjects) cigarette smokers, residing in Jeddah, Saudi Arabia, with an age ran...
Source: Indian Journal of Clinical Biochemistry - February 19, 2018 Category: Biochemistry Source Type: research

Association of Inflammatory and Liver Markers with Cardiometabolic Risk Factors in Patients with Depression
This study aimed to assess the association of inflammatory and liver markers with cardiometabolic risk factors in patients with Depressive disorders. Prevalence of MS by using Modified NCEP ATP-III Criteria and liver enzymes and CRP were assessed in 382 patients with depressive disorders. MS prevalence was 27.7% and lower HDL level was the commonest metabolic abnormality. ALT, GGT, and CRP levels were positively correlated with weight and BMI. ALT, GGT, and CRP levels were significantly greater in patients with abnormal waist circumference, triglyceride levels and raised blood pressure, compared to patients with normal ind...
Source: Indian Journal of Clinical Biochemistry - February 15, 2018 Category: Biochemistry Source Type: research

Sp1 Binding Site Polymorphism at COL1A1 Gene and Its Relation to Bone Mineral Density for Osteoporosis Risk Factor Among the Sikkimese Men and Women of Northeast India
AbstractSingle nucleotide polymorphism in the first intron of Collagen type I alpha 1 (COL1A1) gene which is the binding site of specificity protein 1 (Sp1) transcription factor associated with low bone mineral density and osteoporosis. To evaluate such genetic factors among the Sikkimese population, a total of 150 cases (75 men and 75 women) with primary osteopenia and osteoporosis and 150 healthy controls (75 men and 75 women) of age range between 35 and 65  years were enrolled in this study. The COL1A1 genotypes [SS, Ss and ss] were assessed by restriction enzyme [MscI] digestion of DNA after amplification by polym...
Source: Indian Journal of Clinical Biochemistry - February 7, 2018 Category: Biochemistry Source Type: research

Protective Role of Terminalia bellirica (Gaertn.) Roxb Fruits Against CCl 4 Induced Oxidative Stress and Liver Injury in Rodent Model
AbstractThe medicinal plant,Terminalia bellirica (Gaertn.) Roxb. is widely used in the traditional Indian system of medicine like Ayurveda for centuries in the treatment of various ailments owing to it ’s rejuvenating as well as health promoting effects. The present study evaluates protective role of aqueous acetone extract ofT. bellirica fruits (AATB) against CCl4 induced liver toxicity in animal model. The liver damage was assessed by liver function markers including ALT, AST, ALP, GGT, LDH, total bilirubin, total protein, albumin, globulin and albumin-globulin ratio. The levels of MDA, ROS, and NO along with the t...
Source: Indian Journal of Clinical Biochemistry - February 7, 2018 Category: Biochemistry Source Type: research

Grape Seed Extract Alleviates Dexamethasone-Induced Hyperlipidemia, Lipid Peroxidation, and Hematological Alteration in Rats
In conclusion, grape seed extract exhibited a protective effect in rats against oxidative st ress, hyperlipidemia and hematological alterations induced by dexamethasone. (Source: Indian Journal of Clinical Biochemistry)
Source: Indian Journal of Clinical Biochemistry - February 7, 2018 Category: Biochemistry Source Type: research

Silymarin Attenuates ELMO-1 and KIM-1 Expression and Oxidative Stress in the Kidney of Rats with Type 2 Diabetes
This study was aimed to evaluate the effects of administration of silymarin on oxidative stress markers and ELMO1 and KIM1 expression in the kidney tissue of type 2 diabetic rats. In this experimental study, 36 male Wistar rats were divided into 6 groups: Control, silymarin-treated control (60 and 120  mg/kg/day), diabetic, and silymarin-treated diabetic groups (60 and 120 mg/kg/day). Tissue levels of oxidative stress and biochemical parameters were measured by spectrophotometric methods. Lipid peroxidation levels in the kidney tissue were measured by fluorometric method. Insulin was determined using immunoassay....
Source: Indian Journal of Clinical Biochemistry - February 6, 2018 Category: Biochemistry Source Type: research

Whether Lack of Measurement of Erythrocyte Cholinesterase or Acetyl Cholinesterase Activity Providing us Misleading Information in Organophosphorus Exposure?
(Source: Indian Journal of Clinical Biochemistry)
Source: Indian Journal of Clinical Biochemistry - February 6, 2018 Category: Biochemistry Source Type: research

Immunomodulatory Activity of Sulfonamide Chalcone Compounds in Mice Infected with Filarial Parasite, Brugia malayi
In conclusion, chalcones having direct antifilarial effect also upsurges anti-inflammatory host immune response. Therefore, the results might be envisaged as 4t to be a better a lternative to DEC in the chronic case of lymphatic filariasis. (Source: Indian Journal of Clinical Biochemistry)
Source: Indian Journal of Clinical Biochemistry - January 24, 2018 Category: Biochemistry Source Type: research

Anomalies in MiRNAs Machinery Gene, GEMIN-4 Variants Suggest Renal Cell Carcinoma Risk: A Small Experimental Study from North India
This study comprised 100 patients and 225 controls. Genotyping ofGEMIN4 gene variants was done using Taqman® assay. The association ofGEMIN4 variants and risk prediction of RCC was done by statistical analysis. Haplotype analysis was done to see the combined effect of variants on RCC. Patients carrying variant genotype, CC ofGEMIN4 T/C rs7813 showed significant association whereas in case ofGEMIN4 G/C rs910925 variant genotype, CC significant risk was found.GEMIN4 rs7813 T/C variant genotype, CC showed risk with smoking (p = 0.034). Our study gives a substantive support for the association between theGEMIN4 g...
Source: Indian Journal of Clinical Biochemistry - January 10, 2018 Category: Biochemistry Source Type: research

Influence of β-Globin Haplotypes on Oxidative Stress, Antioxidant Capacity and Inflammation in Sickle Cell Patients of Chhattisgarh
AbstractThe present study was conducted to investigate the frequency of β-globin gene haplotype and their influence on oxidative stress, antioxidant level and inflammation of sickle cell patient of Chhattisgarh. The serum levels of total antioxidant capacity, oxidative stress and inflammation were measured in hundred SCD patients. The mean serum CRP and MDA levels were significantly (p 
Source: Indian Journal of Clinical Biochemistry - January 6, 2018 Category: Biochemistry Source Type: research

Hyperferritinemia in Hemophagocytic Lymphohistiocytosis: A Single Institution Experience in Pediatric Patients
AbstractHemophagocytic lymphohistiocytosis (HLH) is an inflammatory condition that may run a rapid fatal course and calls for prompt diagnosis. Early intervention with steroids and other immunosuppressive drugs can contain the disease process and favours positive outcome. Ferritin ≥500 ng/ml is a HLH diagnostic criterion. We evaluated the diagnostic potential of admission ferritin, in children with HLH. Pediatric patients of a referral teaching hospital from Feb 2010–Oct 2013 having been investigated for ferritin on admission were included. HLH was confirmed when patient s had clinical features and met 5/8 di...
Source: Indian Journal of Clinical Biochemistry - January 1, 2018 Category: Biochemistry Source Type: research

Mutations in HNF1A Gene are not a Common Cause of Familial Young-Onset Diabetes in Iran
AbstractMutations in hepatocyte nuclear factor-1 alpha (HNF1A) as a homeodomain transcription factor which regulates variety of genes, are the most common cause of maturity-onset diabetes of the young (MODY). Detection of HNF1A mutations not only classifies the subtype, but also predicts the likely clinical course and may alters the method of treatment from insulin to the oral sulphonylureas, which is shown to improve glycemic control. The coding and promoter regions ofHNF1A gene were screened for mutations in 34 unrelated Iranian MODY patients. We identified one novel missense mutation (C49G) and two novel polymorphisms a...
Source: Indian Journal of Clinical Biochemistry - January 1, 2018 Category: Biochemistry Source Type: research