Application of Tucatinib and Trastuzumab: Dual Anti HER2 Therapy Against HER2 Positive Breast Cancer
AbstractSynergism in action of tucatinib and trastumab is reported in breast cancer management. However, its molecular basis is yet to be determined. In this context we attempted to provide an explanation at the molecular level by performing in silico experimentation and coupling its result with already available published observations. Our study will provide basis for planning further experimental study for unravelling the truth. (Source: Indian Journal of Clinical Biochemistry)
Source: Indian Journal of Clinical Biochemistry - December 5, 2019 Category: Biochemistry Source Type: research

Investigating Prolonged Neonatal Jaundice: A Staged Approach is Superior and Conducive to Breastfeeding
(Source: Indian Journal of Clinical Biochemistry)
Source: Indian Journal of Clinical Biochemistry - December 4, 2019 Category: Biochemistry Source Type: research

EGFR Mutation Analysis in Non-small Cell Lung Carcinoma Patients: A Liquid Biopsy Approach
In conclusion, our data suggest that evaluation ofEGFR mutation from cfDNA samples is practicable as a non-invasive tool in patients with advanced-stage of lung cancer. (Source: Indian Journal of Clinical Biochemistry)
Source: Indian Journal of Clinical Biochemistry - December 4, 2019 Category: Biochemistry Source Type: research

Genetic Polymorphism of Angiotensin II Type 1 Receptors and Their Effect on the Clinical Outcome of Captopril Treatment in Arab Iraqi Patients with Acute Coronary Syndrome (Mid Euphrates)
This study aims to investigate the impact of genetic polymorphism of AT1R (rs5186 and rs275651) on the ACS outcome in Iraqi patients treated with captopril. A total of 250 Iraqi individuals with ACS were included in this case —control study and they were divided into two study groups; Study group 1 included 125 participants who were prescribed captopril, 25 mg twice daily and study group 2 included 125 participants who received no captopril as part of their ACS treatment (control study). The AT1R gene (rs5186) CC geno type was found to be associated with ST-elevation myocardial infarction (STEMI) (Odd’s ra...
Source: Indian Journal of Clinical Biochemistry - December 2, 2019 Category: Biochemistry Source Type: research

Distribution of Methionine Synthase Reductase (MTRR) Gene A66G Polymorphism in Indian Population
AbstractMethionine synthase reductase (MTRR) is an important enzyme of the folate/homocysteine pathway. It is responsible for regulation of methionine enzyme by reductive methylation. A common variant A66G is reported in the FMN-binding domain of theMTRR gene, which leads to substitution of isoleucine by methionine (I22M) in MTRR enzyme with reduced activity. Reduced catalytic activity of enzyme leads to high homocysteine concentration in blood and increases risk for numerous diseases. The frequency of A66G polymorphism varies in different ethnic groups. The present study has been designed to evaluate the frequency ofMTRR ...
Source: Indian Journal of Clinical Biochemistry - November 30, 2019 Category: Biochemistry Source Type: research

Endothelial Microparticle as an early Marker of Endothelial Dysfunction in Patients with Essential Hypertension: A Pilot Study
AbstractHypertension is a global health burden causing immense morbidity and mortality especially from the complications of end-organ damage. It is expected to affect 29% of the population by the year 2025. Hypertension is usually asymptomatic; it is diagnosed by a disease of exclusion. Numerous factors such as inflammation, oxidative stress, genetic predisposition etc. play roles in the pathogenesis of hypertension. Endothelial microparticles (EMPs) are released into the circulation with the onset of changes in endothelium, even before the release of other routine vascular endothelial markers. EMPs mediate inflammation, t...
Source: Indian Journal of Clinical Biochemistry - November 28, 2019 Category: Biochemistry Source Type: research

Metabolic Bone Profile of Healthy Adult North Indian Population from Chandigarh Urban Bone Epidemiological Study (CUBES)
AbstractWe aimed to estimate metabolic bone profile in a large cohort of healthy, adult Indian population to generate reference standards of serum calcium, phosphate and alkaline phosphatase (ALP), 25 (OH) Vitamin D and iPTH, and also to find out the prevalence of Vitamin D deficiency in healthy population. Apparently healthy people in the age group of 20 –80 years, residing in the union territory of Chandigarh were chosen. Fasting samples for serum calcium, phosphate, albumin, alkaline phosphatase (ALP), 25 (OH) D and iPTH were collected and were processed on the same day. We recruited 930 healthy subjects from...
Source: Indian Journal of Clinical Biochemistry - November 28, 2019 Category: Biochemistry Source Type: research

Anti-Atherosclerotic and Anti-Inflammatory Effects of Curcumin on Hypercholesterolemic Male Rabbits
AbstractCurcumin has a potent antioxidant and anti-inflammatory properties that may suppress inflammatory component of atherosclerosis. It has been demonstrated that curcumin derivatives can reduce the formation of arterial fatty streaks in cholesterol-fed rabbits. Therefore in this study we evaluated the protective effects of Curcumin on the progression of atherosclerosis. 20 mature rabbits were included for this study; they were randomly divided into four groups each of 5. Group 1: (normal control) were fed corn pellets diet and tab water, group 2: (high cholesterol diet control) were kept on cholesterol rich diet (2% ch...
Source: Indian Journal of Clinical Biochemistry - November 19, 2019 Category: Biochemistry Source Type: research

Proceedings: 15th Asia-Pacific Federation for Clinical Biochemistry and Laboratory Medicine (APFCB) Congress 2019 from 17-20 November, 2019, Jaipur, India
(Source: Indian Journal of Clinical Biochemistry)
Source: Indian Journal of Clinical Biochemistry - November 18, 2019 Category: Biochemistry Source Type: research

Why Leadership Skills are Essential for the Future of Laboratory Medicine?
(Source: Indian Journal of Clinical Biochemistry)
Source: Indian Journal of Clinical Biochemistry - October 24, 2019 Category: Biochemistry Source Type: research

A Study on Insulin Levels and the Expression of Glut 4  in Streptozotocin (STZ) Induced Diabetic Rats Treated with Mustard Oil Diet
AbstractThe study was undertaken to evaluate the therapeutic effect of mustard oil incorporated diet in streptozotocin (STZ)-induced type 1 diabetic rats. Dietary composition has shown to play a significant role in improving insulin sensitivity. Various authors have reported the hypoglycemic effect of mustard oil in experimentally induced diabetic rats. In the present study,  reverse transcriptase polymerase chain reaction (RT-PCR) was done to analyze theGlut 4 expression in STZ induced diabetic rats as it is a key player in glucose homeostasis. The effect of mustard oil on serum biochemical parameter and insulin leve...
Source: Indian Journal of Clinical Biochemistry - October 22, 2019 Category: Biochemistry Source Type: research

Expression of FOXP3 and GATA3 Transcription Factors Among Bronchial Asthmatics in Northern Population
AbstractAsthma is a common chronic airways inflammatory disorder in which the expression of genes for the transcription factors FoxP3 and GATA3 plays crucial roles in activation of specific T cells population and pathogenesis of asthma. Recent data have shown that Hb, Eosinophils, total leucocytes count (TLC), absolute eosinophil count (AEC), and IgE, may be involved in adversely influencing the status of several chronic diseases including asthma. In this communication, we have carried out a case control study in order to evaluate the expression of FoxP3, GATA-3 genes in 80 bronchial asthmatic patients using real time poly...
Source: Indian Journal of Clinical Biochemistry - October 21, 2019 Category: Biochemistry Source Type: research

Synergetic Interaction of HLA-DRB1*07 Allele and TNF-Alpha − 863 C/A Single Nucleotide Polymorphism in the Susceptibility to Systemic Lupus Erythematosus
In conclusion, TNF-α − 863A allele (rs1800630) polymorphism is associated with increased risk of nephritis in South Indian SLE patients. We also found an interaction between HLA-DRB1*07 allele with TNF-α − 863 C/A promoter polymorphism giving supportive evidence for the tig ht linkage disequilibrium between TNF-α promoter SNPs and MHC class II DRB1 alleles. (Source: Indian Journal of Clinical Biochemistry)
Source: Indian Journal of Clinical Biochemistry - October 18, 2019 Category: Biochemistry Source Type: research

New Horizons in Correction of Mutated ATP7B in Wilson Disease Using Pharmacological Agents: Precise Medicine
(Source: Indian Journal of Clinical Biochemistry)
Source: Indian Journal of Clinical Biochemistry - October 16, 2019 Category: Biochemistry Source Type: research

Progeria: A Rare Genetic Syndrome
AbstractAn uncommon deadly genetic situation symbolized by the presence of rapid maturation in infants is called as the Hutchinson –Gilford Progeria Syndrome. The term basically is meant as ‘prematurely old’ taken from the Greek meanings. The selective cause behind this syndrome is usually a mutation in a gene called LMNA. The product of this LMNA gene which is a protein i.e. Lamin-A is considered to be responsible for an atomical framing which clasps the nuclei of the cell, well organized and together. But, the recent investigations prove a deformity in the protein i.e. Lamin-A that leads to the non-stab...
Source: Indian Journal of Clinical Biochemistry - September 25, 2019 Category: Biochemistry Source Type: research

A Study to Assess and Correlate Serum Lipid Profile with Reaction Time in Healthy Individuals
AbstractDyslipidaemia, metabolic syndrome, diabetes mellitus and arterial hypertension contribute to the risk of cognitive disorder and dementia. However very few and conflicting results are available with respect to the impact of lipoprotein ratios on cognitive functions in healthy individuals. Therefore, this study was aimed to investigate the association of lipid profile and its ratios with reaction time in young healthy individuals. 89 individuals (54 male and 35 female) with mean age of 31.20  ± 3.19 years were selected for this observational study conducted at AIIMS Rishikesh and written con...
Source: Indian Journal of Clinical Biochemistry - September 18, 2019 Category: Biochemistry Source Type: research

A Novel Mutation in ACAT1 Causing Beta-Ketothiolase Deficiency in a 4-Year-Old Sri Lankan Boy with Metabolic Ketoacidosis
AbstractBeta-ketothiolase (mitochondrial acetoacetyl-CoA thiolase, T2) deficiency is a rare genetic disorder of ketone utilization and isoleucine catabolism caused by mutations in theACAT1 gene. Here we report the first Sri Lankan case of T2 deficiency confirmed by genetic analysis. A 4-year-old boy presented with the first episode of severe metabolic ketoacidosis after a febrile illness. On admission, the child was drowsy and had circulatory collapse needing intubation. Initial investigations were not detective of a cause and symptomatic management did not improve the condition. During the acute episode, his urine organic...
Source: Indian Journal of Clinical Biochemistry - September 16, 2019 Category: Biochemistry Source Type: research

The Nephroprotective Effect of Lycopene on Renal Ischemic Reperfusion Injury: A Mouse Model
This study aims to investigate the possible nephroprotective effect of lycopene on renal ischemic–reperfusion injury in mice model. Forty Swiss Albino adult male mice were randomly allocated onto one of the four study groups: sham group: mice had median laparotomy under anesthesia with no procedures performed, renal tissues and blood samples were collected. ischemic–reperfusion group (I–R-control): mice underwent median laparotomy under anesthesia, followed by 30 min bilateral renal ischemia. Renal tissues and blood samples were co llected after 2 h from reperfusion. Vehicle-treated group: mice ...
Source: Indian Journal of Clinical Biochemistry - September 13, 2019 Category: Biochemistry Source Type: research

Association of Genetic Variants with Hyperhomocysteinemia in Indian Patients with Thrombosis
AbstractHyperhomocysteinemia known to be associated with increased thrombotic tendency has been considered as a risk factor for coronary artery disease, atherosclerosis, venous thrombosis, and stroke. There are three main genes MTHFR, cystathionine beta-synthase (CBS) and methionine synthase (MS) and it's genetic variant that are known to influence the homocysteine metabolism leading to hyperhomocysteinemia. There is scarcity of Indian data on hyperhomocysteinemia and genetics variants in patients with thrombosis. Hence the objective of present study was to determine MTHFR, CBS, and MS genetic variants in thrombosis patien...
Source: Indian Journal of Clinical Biochemistry - August 30, 2019 Category: Biochemistry Source Type: research

Carvacrol Downregulates Lysyl Oxidase Expression and Ameliorates Oxidative Stress in the Liver of Rats with Carbon Tetrachloride ‐Induced Liver Fibrosis
AbstractIn the current study, we aimed to investigate the effect of carvacrol on the suppression of liver fibrosis progression through targeting lysyl oxidase (LOX) expression. The rats received carbon tetrachloride (CCl4) intraperitoneally and carvacrol orally for 10  weeks. Liver damage was evaluated by measuring the serum level of alanine aminotransferase, aspartate aminotransferase and alkaline phosphatase and hepatic oxidative stress parameters including total antioxidant capacity, total thiol group and total oxidant status spectrophotometry and malondialde hyde fluorometrically. Extracellular deposition of colla...
Source: Indian Journal of Clinical Biochemistry - August 30, 2019 Category: Biochemistry Source Type: research

A Study on Impact of BPA in the Adipose Tissue Dysfunction (Adiposopathy) in Asian Indian Type 2 Diabetes Mellitus Subjects
AbstractA surge to increase the production via usage of chemicals at both industrial and agricultural arena has forced humans to be routinely and imprudently exposed to a wide variety of endocrine disrupting chemicals. The overall aim of the study was to evaluate possible relation that might exist between bisphenol-A (BPA) and the adipose tissue hormones, and further impact on adiposopathy. In the present study, the role of BPA, an “endocrine disruptor” with respect to adiposopathy was evaluated in type 2 diabetes mellitus patients. For the study, 150 healthy control subjects and 150 newly diagnosed diabetes pa...
Source: Indian Journal of Clinical Biochemistry - August 3, 2019 Category: Biochemistry Source Type: research

MicroRNAs and Diseases: Promising Biomarkers for Diagnosis and Therapeutics
(Source: Indian Journal of Clinical Biochemistry)
Source: Indian Journal of Clinical Biochemistry - July 19, 2019 Category: Biochemistry Source Type: research

Cinnamon Attenuated Long-Term IGT-Induced Retinal Abnormalities via Regulation of Glucose Homeostasis in Neonatal Streptozotocin Induced Rat Model
In conclusion, Cinnamon attenuated IGT induced retinal abnormalities probably through its hypoglycemic property. (Source: Indian Journal of Clinical Biochemistry)
Source: Indian Journal of Clinical Biochemistry - July 9, 2019 Category: Biochemistry Source Type: research

Amino Acid Profile and Lactate Pyruvate Ratio: Potential Adjunct Markers for Differentiating Inborn Errors of Metabolism
The objective of this study is not to compete with the existing gold standard metho dology rather makes the best of basic diagnostic modalities available. Five cases out of 100 referred cases of IEM suspicion showed the altered pattern of Aminogram correlating with high L/P ratio and Hyperammonemia, which were positive cases, as confirmed by the clinician who referred. Hence study parameters could be used as preliminary protocol to investigate and screen the IEM. (Source: Indian Journal of Clinical Biochemistry)
Source: Indian Journal of Clinical Biochemistry - July 5, 2019 Category: Biochemistry Source Type: research

Cadmium in Human Diseases: It ’s More than Just a Mere Metal
AbstractCadmium (Cd), poisoning has been reported from all around the World, causing many deaths annually. Cd is a toxic heavy metal, and is widely present in environment. It has been reported that chronic  Cd exposure is associated with kidney disease, osteoporosis, cardiovascular diseases and cancer. Smoking causes exposure to significantly higher Cd levels in humans. Tobacco smoke transports Cd into the lungs. Blood then transport it to the rest of the body where it increases effects by potentiati ng Cd that is already present from Cd-rich food. Other high exposures of Cd can occur with people, who live near hazard...
Source: Indian Journal of Clinical Biochemistry - June 27, 2019 Category: Biochemistry Source Type: research

Screening and Assessment of Bone Health in Indian Women Using an Indigenous ELISA of Human Osteocalcin a Bone Turnover Marker
AbstractOsteoporosis a major public health problem of the elderly, is associated with substantial morbidity and socio economic burden. The aim of the study was to screen women with low bone mass using the indigenously developed Osteocalcin (OC) ELISA kit and compare it with commercial ELISA kit and evaluate. The diagnostic potential of the assay was assessed in 359 samples from neighboring tertiary care hospitals over a period of 2  years. OC levels were estimated by the developed indigenous assay in samples, correlated with the Bone Mineral Density (BMD) measurements and compared by a commercial ELISA kit. On the bas...
Source: Indian Journal of Clinical Biochemistry - June 25, 2019 Category: Biochemistry Source Type: research

Modes of Calcium Regulation in Ischemic Neuron
AbstractCalcium (Ca2+) dysregulation is a major catalytic event. Ca2+ dysregulation leads to neuronal cell death and brain damage result in cerebral ischemia. Neurons are unable in maintaining calcium homeostasis. Ca2+ homeostasis imbalance results in increased calcium influx and impaired calcium extrusion across the plasma membrane. Ca2+ dysregulation is mediated by different cellular and biochemical mechanism, which leads to neuronal loss resulting stroke/cerebral ischemia. A better understanding of the Ca2+ dysregulation might help in the development of new treatments in order to reduce ischemic brain injury. An optimal...
Source: Indian Journal of Clinical Biochemistry - June 20, 2019 Category: Biochemistry Source Type: research

Assessing Diagnostic Accuracy of Serum Holotranscobalamin (Active-B12) in Comparison with Other Markers of Vitamin B12 Deficiency
AbstractAbout 15 –40% India is Vitamin B12 deficient (commonly diagnosed by total Vitamin B12) but, as only holoTC (active form) is taken up by body cells, thus measuring holoTC is more reflective of Vitamin B12 status than the former. We aimed to assess diagnostic accuracy of serum holoTC in comparison wit h total Vitamin B12 and total Homocysteine (HCY) as indicator of serum Vitamin B12 status. 217 human subjects (99 males and 118 females) ranging from 17 to 83 years were divided into Vitamin B12 deficient (n = 70), borderline (n = 100) and sufficient groups (n = 47)...
Source: Indian Journal of Clinical Biochemistry - June 12, 2019 Category: Biochemistry Source Type: research

Assessment of DNA Methylation in p15, p16 and E-Cadherin  Genes as a Screening Tool for Early Carcinoma Cervix
AbstractCancer cervix is diagnosed late in women due to anatomical inaccessibility of the area. Hence, a robust screening strategy will help detect carcinoma cervix early which will significantly decrease the mortality and morbidity due to this disease. We evaluated DNA methylation of three tumour suppressor genes p15, p16 and E-Cadherin on cervical smears to assess DNA methylation as a screening tool for detection of early cervical cancer in comparison to PAP smears. DNA was extracted from cervical smears of 20 cases and 30 controls. The DNA was bisulphite modified. Methylation specific PCR (MSP) was performed to assess t...
Source: Indian Journal of Clinical Biochemistry - June 12, 2019 Category: Biochemistry Source Type: research

Burden of Inherited Hematological Disorders in Population of Vindhyan Region, Madhya Pradesh
(Source: Indian Journal of Clinical Biochemistry)
Source: Indian Journal of Clinical Biochemistry - June 11, 2019 Category: Biochemistry Source Type: research

Evaluation of Silencing Effect of miR-133a-5p Mimic on TIM-3 Expression in AML (HL-60) Cell Line
In this study, the silencing effect of miR-133a-5p on TIM-3 expression in AML cell lineage (HL-60) was investigated. It ’s been hypothesized that miR-133a-5p may suppress the TIM-3 expression in AML cell line. Initially, miRNA-TIM-3 prediction, enrichment, and network analysis were done. Then, miR-133a-5p mimic was transfected into HL-60 cells. The TIM-3 protein and gene expression were measured by flow cytometry a nalysis and real-time PCR, respectively. MTT assay was also carried out. Based on the Bioinformatics predictions, miR-133a-5p was able to silence TIM-3 expression. Also, significant pathways pertained to m...
Source: Indian Journal of Clinical Biochemistry - June 4, 2019 Category: Biochemistry Source Type: research

Antihyperglycemic Potential of Back Tea Extract Attenuates Tricarboxylic Acid  Cycle Enzymes by Modulating Carbohydrate Metabolic Enzymes in Streptozotocin-Induced Diabetic Rats
AbstractThe present study was aimed to investigate the effect of black tea extract on blood glucose, plasma insulin, Hemoglobin, carbohydrate metabolic enzymes and tricarboxylic enzymes in streptozotocin (STZ) induced diabetic rats. Diabetes was induced in male albino Wistar rats by intraperitoneal administration of STZ (40  mg/kg b wt). Black tea extract was administered to diabetic rats at a dose of 25, 50 and 100 mg/kg body weight for 30 days. The effects of black tea extract on glucose, insulin and HbA1c levels were analyzed to confirm the effective dose. Administration of black tea extract to ...
Source: Indian Journal of Clinical Biochemistry - May 16, 2019 Category: Biochemistry Source Type: research

Association of Common Single Nucleotide Polymorphisms of Candidate Genes with Gallstone Disease: A Meta-Analysis
AbstractNumerous studies have investigated the relationship between various candidate gene polymorphisms and gallbladder stone disease (GSD) across ethnic populations; however, the results are often inconsistent. This meta-analysis aims to comprehensively evaluate the influence of common ABCG8 T400K, ABCG8 D19H, ABCG8 C54Y, ApoB100 EcoRI, ApoB100 XbaI, ApoE HhaI, CETP TaqI, CYP7A1 Bsa, LRPAP1 I/D and TNF- α A308G polymorphisms on the risk of gallbladder stone disease. 33 Full-text articles with 9250 cases and 12,029 healthy controls (total 21,279 subjects) were analyzed using the RevMan software (V5.1) and the Compre...
Source: Indian Journal of Clinical Biochemistry - May 15, 2019 Category: Biochemistry Source Type: research

Therapeutic Potential of Dihydropyridine Calcium Channel Blockers on Oxidative Injury Caused by Organophosphates in Cortex and Cerebellum: An In Vivo Study
This study was designed to investigate the effects of amlodipine (AM), a dihydropyridine calcium channel blocker, on the oxidative damage induced by diazinon (DZN) in the rat cortex and cerebellum. Forty-two rats were randomly divided into six groups. The rats were treated intraperitoneally with normal saline (group 1), AM (9  mg/kg; group 2), DZN (32 mg/kg; group 3) and different doses of AM (3, 6, and 9 mg/kg; groups 4, 5, and 6, respectively) with DZN. After 14 days, the cerebellum and cortex tissues were removed for biochemical and histological experiments. DZN significantly decreased acetylcholines...
Source: Indian Journal of Clinical Biochemistry - May 14, 2019 Category: Biochemistry Source Type: research

A Revisit to a Qualitative Method for the Detection of Organophosphorus Pesticides
(Source: Indian Journal of Clinical Biochemistry)
Source: Indian Journal of Clinical Biochemistry - May 14, 2019 Category: Biochemistry Source Type: research

Novel Direction in Mechanisms Underlying Lead Toxicity: Evidence and Prospective
(Source: Indian Journal of Clinical Biochemistry)
Source: Indian Journal of Clinical Biochemistry - April 10, 2019 Category: Biochemistry Source Type: research

Clinical Importance of Estrogen Receptor 1 ( ESR1 ) Gene Polymorphisms and Their Expression Patterns in Coronary Artery Disease Patients: A Study from India
In conclusion, this study demonstrates a significantly heightened risk of CAD associated with the inheritance of mutant genotypes of ESR1 -397T>C and -351A>G gene polymorphisms, in the north Indian population. This is the first report of a lowered ER α mRNA expression in conjunction with the presence of mutant ‘C’ allele of ESR1 -397T>C polymorphism with consequent increased CAD susceptibility. (Source: Indian Journal of Clinical Biochemistry)
Source: Indian Journal of Clinical Biochemistry - April 5, 2019 Category: Biochemistry Source Type: research

Pathogenic and Therapeutic Role of H3K4 Family of Methylases and Demethylases in Cancers
AbstractHistone modifications occupy an essential position in the epigenetic landscape of the cell, and their alterations have been linked to cancers. Histone 3 lysine 4 (H3K4) methylation has emerged as a critical epigenetic cue for the regulation of gene transcription through dynamic modulation by several H3K4 methyltransferases (writers) and demethylases (erasers). Any disturbance in the delicate balance of writers and erasers can result in the mis-regulation of H3K4 methylation, which has been demonstrated in several human cancers. Therefore, H3K4 methylation has been recognized as a putative therapeutic or prognostic ...
Source: Indian Journal of Clinical Biochemistry - April 3, 2019 Category: Biochemistry Source Type: research

Anti-diabetic Effect of Emblica - officinalis (Amla) Against Arsenic Induced Metabolic Disorder in Mice
AbstractChronic exposure to arsenic through drinking water and occupational exposure has been found to be associated with the diabetic symptoms. Earlier, we reported that arsenic induced enhanced oxidative stress, inflammation, dislipidemia and hepatotoxicity in mice have been protected by treatment withEmblica officinalis (amla). The present study has therefore been focused to investigate the efficacy of amla in mitigation of arsenic induced hyperglycemia in mice. Arsenic exposure (3  mg/kg b.w./day for 30 days) in mice altered glucose homeostasis and significantly decreases hepatic glucose regulatory enzyme, gl...
Source: Indian Journal of Clinical Biochemistry - March 25, 2019 Category: Biochemistry Source Type: research

Evaluation of Anti-hypertrophic Potential of Enicostemma littorale Blume on Isoproterenol Induced Cardiac Hypertrophy
AbstractThe main objective of this study is to evaluate the anti-hypertrophic potential of the aqueous extract ofEnicostemma littorale (E. littorale) against isoproterenol induced cardiac hypertrophic rat models (male albino Wistar rats) through biochemical investigations. Aqueous extract ofE. littorale known for various beneficial properties was administered (100  mg/kg, 12 days, oral) to isoproterenol (ISO) induced cardiac hypertrophic rats (low ISO—60 mg/kg, 12 days and high ISO—100 mg/kg, 12 days, subcutaneous) and were compared with group that was treated with the reference dr...
Source: Indian Journal of Clinical Biochemistry - March 22, 2019 Category: Biochemistry Source Type: research

The Upregulation of hsa-mir-181b-1 and Downregulation of Its Target CYLD in the Late-Stage of Tumor Progression of Breast Cancer
This study aimed to investigate the expression levels of hsa-mir-181b-1 andCYLD in a cohort of breast tumor tissues and normal adjacent tissues to assess their association with breast cancer stages. A total number of 60 breast samples including cancerous and normal adjacent tissue specimens were collected. After pathological study, the expression of hsa-mir-181b-1 andCYLD were measured by qRT-PCR method. The hsa-mir-181b-1 expression level was significantly increased in breast tumor tissues compared to the controls. This increase was associated with the disease progression. Conversely,CYLD expression level was decreased in...
Source: Indian Journal of Clinical Biochemistry - March 21, 2019 Category: Biochemistry Source Type: research

Amelioration of Combination of Paclitaxel and Di Allyl Sulfide on the Alterations of Bcl2, P53 and Apoptosis Changes Against 7,12 Di Methyl Benz (A) Anthracene Induced Skin Cancer in Experimental Animals
AbstractThe purpose of this study was to investigate the Bcl2, P53 and apoptosis changes against skin cancer in experimental animals. Skin cancer is the most common form of human cancer. It is estimated that over 1 million new cases occur annually. The annual rates of all forms of skin cancer are increasing each year, representing a growing public concern. It has also been estimated that nearly half of all Americans who live to age 65 are likely to develop skin cancer at least once. Skin cancer was induced in rats by Di Methyl Benz (a) Anthracene at the dosage of DMBA (5  µg) per animal, three times a week for 2...
Source: Indian Journal of Clinical Biochemistry - March 15, 2019 Category: Biochemistry Source Type: research

The Effect of miR-98 and miR-214 on Apoptotic and Angiogenic Pathways in Hepatocellular Carcinoma HepG2 Cells
This study were conducted to explore the effect of these two miRNAs on some apoptotic and angiogenic genes namely, BCL-2, survivin, CCND1, CDC2, P53 and P21, VEGF, Hif-1 α, MMP-2, MMP-9, Ang-1, Ang-2, and FGF-1. miRNAs mimics and inhibitors transfection was used to investigate the role of both studied molecules in apoptosis and angiogenesis in HepG2 cells. QRT-PCR was used for Quantitative gene and miRNA expression analyses. The study revealed that miR-98 could ser ve as a pro-apoptotic factor through the upregulation of P53 gene expression levels. Besides, the anti-angiogenic effect of this miRNA was evident through...
Source: Indian Journal of Clinical Biochemistry - March 11, 2019 Category: Biochemistry Source Type: research

The Hemolyzed Sample: To Analyse Or Not To Analyse
AbstractPreanalytical errors constitute about 40 –65% of laboratory errors, of which 60% are due to hemolysis. This leads to imprecise reporting and misinterpretation of the actual concentration of analytes. Hence the aim of this study was to estimate the extent of different degrees of interference by visible hemolysis. 25 hemolysed sampl es along with their fresh unhemolysed sample were studied. Hemolyzed serum was mixed with unhemolyzed serum in predefined serial ratios from 100%, 70%, 50%, 30% and 10% to achieve different grades of hemolysis. Each dilution was analysed for BUN, creatinine, uric acid, phosphorus,...
Source: Indian Journal of Clinical Biochemistry - March 11, 2019 Category: Biochemistry Source Type: research

Assessment of Garcinia pedunculata Roxb. Role on Biological Markers in Carbon Tetra Chloride Induced Rat Model
AbstractGarcinia pedunculata Roxb. is an important medicinal plant of North Eastern (NE) region of India, having number of medicinal properties and used against various diseases in folk medicine. An empirical research was designed to carry out evaluation of hepatoprotective activity of the leaves ofG. pedunculata with special reference to its putative protective role. Methanolic extract from the dried leaf powder ofG. pedunculata Roxb. was prepared by hot continuous extraction method. The prepared extract was investigated at different dose levels for its hepatoprotective nature and further histopathological study was carri...
Source: Indian Journal of Clinical Biochemistry - March 6, 2019 Category: Biochemistry Source Type: research

In Vivo Validation of Folkloric Use of Costus pictus D. Don as Antidiabetic Plant in Assam, Using Streptozotocin Induced Wister Rat Model
AbstractCostus pictus D. Don belonging to the family Costaceae is one of the most commonly used plant among traditional healers in the upper Assam region in India, specifically used in the treatment of diabetes. Aerial parts of the plant are said to have potent anti diabetic property. The present study was aimed to evaluate the traditionally claimed antidiabetic activity of aerial parts ofC. pictus in animal models. Healthy male Wister rats (120  ± 30 gm) were used in the study and diabetes was induced by Streptozotocin (STZ) i.p. prepared by dissolving in citrate buffer (pH 4.5), along with nicotinami...
Source: Indian Journal of Clinical Biochemistry - March 6, 2019 Category: Biochemistry Source Type: research

A Study on Association Between Protein Carbonyl and Anti-cyclic Citrullinated Peptide Antibody in Rheumatoid Arthritis: Introducing a New Supplementary Biomarker
AbstractRedox state and immune mechanisms are two major factors implicated in rheumatoid arthritis (RA). Regarding some limitations of anti-cyclic citrullinated peptide (anti-CCP) antibody in RA diagnosis, recruiting another strong marker of oxidative stress could lead to more definitive diagnosis. To evaluate the potential of protein carbonyl content as a supplementary biomarker for RA. Eighty patients with RA attending the Research Center from 2015 to 2016 were recruited in this study. Smoker and alcoholic subjects, or those with any other systemic illness were excluded from the study. Demographic information and clinica...
Source: Indian Journal of Clinical Biochemistry - March 5, 2019 Category: Biochemistry Source Type: research

Evaluation of Relationship Between Arylesterase-Based Activity and Genetic Variants of Paraoxonase1 in T2DM Patients within Golestan Province
AbstractArylesterase activity of Paraoxonase-1 (PON1) enzyme may be play important role in initiation and progression of several diseases. Activity or serum level of Arylesterase can be affected by many genetic alterations such as SNPs. The reduction in the activity and serum level of Arylesterase could be involved in Type2 diabetes mellitus (T2DM). The aim of this investigation is to examine the association between Arylesterase activity and promoter polymorphism ( − 108C >  T) of PON1gene in patients with T2DM in Golestan Province, northern area of Iran. Achievement of this purpose was due to...
Source: Indian Journal of Clinical Biochemistry - March 5, 2019 Category: Biochemistry Source Type: research

Angiogenic Potential, Circulating Angiogenic Factors and Insulin Resistance in Subjects with Obesity
AbstractAltered vascular function and pathological angiogenesis are important factors common to the development of obesity and obesity-associated diseases. Most human studies relating obesity and angiogenesis have compared levels of angiogenic factors in obesity without looking at the serum angiogenic capacity which reflects the balance between the effects of angiogenic and angiostatic factors. Therefore, in this cross-sectional study, the serum angiogenic potential and levels of angiogenic factors in serum of obese (BMI  >  25 kg/m2) and lean subjects (BMI  
Source: Indian Journal of Clinical Biochemistry - February 22, 2019 Category: Biochemistry Source Type: research

Systematic Differences Between Total and Free Prostate-Specific Antigen Immunoassays: Comparison Using Passing and Bablok Regression
In this study we analyzedintermethod differences in total PSA (tPSA) and free PSA(fPSA) measurement using ARCHITECTi2000SR (Abbott Diagnostics) and COBAS E601 (Roche Diagnostics). A number of 160 blood samples were tested for tPSA and 50 samples for fPSA (selecting only sampleswith tPSA: 4.1 –10.0 μg/L). Passing–Bablok regression analysis was used to compare the two analytical methods fortPSA, fPSA and percentage of fPSA (%fPSA). A strong correlation was noticed between ARCHITECTi2000SR and COBAS E601 for tPSA, fPSA and %fPSA (r between 0.94 and 0.99). Concentrations of tPSA and fPSA measured by COBAS E...
Source: Indian Journal of Clinical Biochemistry - February 13, 2019 Category: Biochemistry Source Type: research