Differential Iron Status and Trafficking in Blood and Placenta of Anemic and Non-anemic Primigravida Supplemented with Daily and Weekly Iron Folic Acid Tablets
AbstractThe molecular mechanism of iron transfer across placenta in response to maternal anemic status/ iron supplementation is not clear. We hypothesized that maternal iron/ anemia status during early trimesters can be utilized as a biomarker tool to get estimates of placental iron status. Early interventions can be envisaged to maintain optimum placental/ foetal iron levels for healthy pregnancy outcomes. One hundred twenty primigravida were recruited and divided into non-anemic and anemic group on the basis of hemoglobin levels. The groups were randomly allocated to receive daily and weekly iron folic acid (IFA) tablets...
Source: Indian Journal of Clinical Biochemistry - October 5, 2018 Category: Biochemistry Source Type: research

Stem Cell Biology: A New Hope in Regenerations and Replenishments Therapy
(Source: Indian Journal of Clinical Biochemistry)
Source: Indian Journal of Clinical Biochemistry - October 1, 2018 Category: Biochemistry Source Type: research

Association of Vitamin D Receptor (FokI and BsmI) Gene Polymorphism with Bone Mineral Density and Their Effect on 25-Hydroxyvitamin D Level in North Indian Postmenopausal Women with Osteoporosis
AbstractOsteoporosis is a systemic disease with a strong genetic component. Vitamin D receptor (VDR) has been suggested as a candidate gene for osteoporosis. Therefore the present study was aimed to investigate the pattern of allelic variants of VDR gene polymorphism (FokI and BsmI), its influence on vitamin D levels and bone mineral density (BMD) in North Indian postmenopausal women with osteoporosis for possible genetic association. 254 postmenopausal osteoporotic women and 254 postmenopausal non osteoporotic women were included in the study. VDR FokI and BsmI gene polymorphism gene were assessed by the PCR-RFLP method. ...
Source: Indian Journal of Clinical Biochemistry - October 1, 2018 Category: Biochemistry Source Type: research

Neurochemical and Behavioral Dysfunctions in Pesticide Exposed Farm Workers: A Clinical Outcome
AbstractThe problem of pesticides is not new and its exposure to human due to indiscriminate use is largely associated with the health related problems including neurotoxicological alterations. High levels of pesticide residues and their metabolites in the dietary constituents, food materials, maternal blood, cord blood, placenta breast milk have been reported and linked to alterations in birth weight, crown heel length, head circumference, mid-arm circumference and ponderal index of the neonates. Epidemiological studies have suggested that exposure of pesticide to human could be a significant risk factor for neurological ...
Source: Indian Journal of Clinical Biochemistry - October 1, 2018 Category: Biochemistry Source Type: research

Monotonic Dose Effect of Bisphenol-A, an Estrogenic Endocrine Disruptor, on Estrogen Synthesis in Female Sprague-Dawley Rats
AbstractBisphenol-A (BPA) is a ubiquitous environmental chemical that produces adverse effect on reproduction system due to its potent estrogenic endocrine disruptive activity. The present study was aimed to investigate the monotonic dose effect of BPA on estrogen synthesis in female Sprague-Dawley rats. For this purpose, we administered three different doses of BPA (10, 50, 100  µg/kg bw/day) into rats and analyzed various biochemical, hormonal, molecular and histological parameters. 10 µg BPA treated rats showed significantly decreased levels of phase I detoxification agents (CYP450, Cyt-b5). Overex...
Source: Indian Journal of Clinical Biochemistry - October 1, 2018 Category: Biochemistry Source Type: research

Thiamine Deficiency Induced Dietary Disparity Promotes Oxidative Stress and Neurodegeneration
AbstractThiamine or vitamin B1 is a well known coenzyme and nutrient necessary for the assembly and right functioning of several enzymes involved in the energy metabolism. The present study evaluates oxidative stress and prevalence of neurodegenerative conditions in the brain following TD. The study was carried out on mice (Musmusculus) in three groups, namely control and thiamine-deficient group for 8 (TD 8) and 10 (TD 10) days. Lipid peroxidation was determined in terms of reduced glutathione (GSH) and thiobarbituric acid reactive substance (TBARS). The level of antioxidant enzymes such as catalase (CAT), glutathione red...
Source: Indian Journal of Clinical Biochemistry - October 1, 2018 Category: Biochemistry Source Type: research

Increased Nuclear Factor- κB/RelA Expression Levels in Human Colorectal Carcinoma in North Indian Patients
AbstractColorectal cancer is a major cause of cancer-related death in many countries. Inflammatory pathway is considered to play a major role in colorectal carcinogenesis. Nuclear factor kappa B (NF- κB) pathway is a link between inflammation and cancer. NF-κB is a transcription factor which belongs to the Rel family. Activation of NF-κB has been shown to play a role in cell proliferation, apoptosis, cytokine production, and oncogenesis. The aim of the present study was to evaluate the expres sion levels of NF-κB/RelA in colorectal carcinoma using Real-time PCR. For this study, tumor tissue was take...
Source: Indian Journal of Clinical Biochemistry - October 1, 2018 Category: Biochemistry Source Type: research

Citral, a Monoterpene Inhibits Adipogenesis Through Modulation of Adipogenic Transcription Factors in 3T3-L1 Cells
In this study, we investigated the inhibitory effect of citral against adipogenic genes in 3T3-L1 cells. The mouse fibroblast 3T3-L1 pre-adipocytes were differentiated into adipocytes using adipogenic cocktail (5  g/ml insulin, 0.5 mM isobutylmethylxanthine and 10 M dexamethasone). Differentiation of adipocytes was evaluated by assessing triglyceride accumulation assay and cell viability by MTT assay. The PI3K/AKT signaling, adipogenic specific transcription factors (PPARγ, SREBP-1c, FAS and CPD) and inf lammatory genes (TNF-α, IL-6 and MCP-1) were analyzed by western blotting and reverse transcr...
Source: Indian Journal of Clinical Biochemistry - October 1, 2018 Category: Biochemistry Source Type: research

Serum Biomarker Based Algorithms in Diagnosis of Ovarian Cancer: A Review
AbstractEpithelial ovarian cancer accounts for more than 90% of ovarian tumours and continues as a leading cause of death from gynaecological malignancies. It is often difficult to differentiate a benign ovarian mass from malignant ones. Invasive histopathological biopsy is used as the gold standard diagnostic tool to diagnose cancer in patients with ovarian mass. A wide spectrum of Biomarkers were tried in various studies to develop a non invasive diagnostic tool, out of which HE4 and CA 125 remain the only clinically useful biomarker. Consequently various Biomarker based algorithms i.e. Risk of Malignancy Index, risk of ...
Source: Indian Journal of Clinical Biochemistry - October 1, 2018 Category: Biochemistry Source Type: research

Cytochrome P450 Genes (CYP2E1 and CYP1A1) Variants and Susceptibility to Chronic Hepatitis B Virus Infection
AbstractHepatitis B virus (HBV) infection is a worldwide health concern which is associated with significant morbidity and mortality. Both viral and host factors have a significant effect on infection, replication and pathogenesis of HBV. The aim of this study was to investigate the effect ofCYP2E1 andCYP1A1 genetic variants on susceptibility to HBV. 143 individuals including 54 chronic HBV patients and 89 healthy controls were enrolled in the genotyping procedure. rs2031920 and rs3813867 atCYP2E1 as well as rs4646421 and rs2198843 atCYP1A1 loci were studied in all subjects using PCR –RFLP (restriction fragment lengt...
Source: Indian Journal of Clinical Biochemistry - October 1, 2018 Category: Biochemistry Source Type: research

Hypocalcemia in an 11  Year Old Child: A Difficult Case to Treat
We report a unique case of an eleven year old male child who presented with chronic kidney disease associated with severe hypocalcemia, tonic-clonic seizures, hypovitaminosis D but normal electroencephalogram and electrocardiography. The child required prolonged intravenous calcium gluconate therapy to correct his ionised calcium levels. (Source: Indian Journal of Clinical Biochemistry)
Source: Indian Journal of Clinical Biochemistry - October 1, 2018 Category: Biochemistry Source Type: research

Whether Lack of Measurement of Erythrocyte Cholinesterase or Acetyl Cholinesterase Activity Providing us Misleading Information in Organophosphorus Exposure?
(Source: Indian Journal of Clinical Biochemistry)
Source: Indian Journal of Clinical Biochemistry - October 1, 2018 Category: Biochemistry Source Type: research

Can High Titres of Anti Tissue Transglutaminase Antibodies Reduce the Need for Intestinal Biopsy for Diagnosis of Celiac Disease?
This study confirms that a small intestinal biopsy is not always necessary for the diagnosis of CD in symptomatic patients with high tTGA levels (>70  IU/ml). (Source: Indian Journal of Clinical Biochemistry)
Source: Indian Journal of Clinical Biochemistry - October 1, 2018 Category: Biochemistry Source Type: research

Stem Cell Biology: A New Hope in Regenerations and Replenishments Therapy
(Source: Indian Journal of Clinical Biochemistry)
Source: Indian Journal of Clinical Biochemistry - October 1, 2018 Category: Biochemistry Source Type: research

Hepcidin and Ferritin: Important Mediators in Inflammation Associated Anemia in Systemic Lupus Erythematosus Patients
AbstractSystemic Lupus Erythematosus is an autoimmune disease with female preponderance. Anemia is found in 50% of Systemic Lupus Erythematosus patients. This is a cross sectional case control study with 30 female Systemic Lupus Erythematosus patients having inflammation associated anemia (Hemoglobin  
Source: Indian Journal of Clinical Biochemistry - October 1, 2018 Category: Biochemistry Source Type: research

Hesperetin on Cell Surface Glycoconjugates Abnormalities and Immunohistochemical Staining with Cytokeratin in 7,12 Dimethylbenz(a)anthracene Induced Hamster Buccal Pouch Carcinogenesis
AbstractHesperetin, a naturally occurring citrus flavanone of the bioactive substance, possesses different pharmacological and biochemical activities including anti-cancer and anti-oxidants effect. The aim of the study to investigate that hesperetin on abnormalities of glycoconjugates (protein bound hexose, hexosamine, total sialic acid and fucose), histology (PAS staining) and immunoexpression of cytokeratin during 7,12-dimethylbenz(a)anthracene (DMBA) induced hamster buccal pouch (HBP) carcinogenesis. Oral tumors were developed in the buccal pouches of male golden Syrian hamsters by topical application of 0.5% DMBA thric...
Source: Indian Journal of Clinical Biochemistry - October 1, 2018 Category: Biochemistry Source Type: research

Ophthalmic Presentation of Disseminated Tuberculosis with Relapse-Immunological Profile
AbstractTB as the cause of uveitis varies from 0.5 to 10.5%; low sensitivity of confirmatory laboratory investigations and inconsistency of diagnostic criteria leads to paucity of data. Diagnosis requires a high level of suspicion and is often presumptive based on indirect evidences. Interferon gamma, Interleukin-2 and Neopterin are key biomarkers in immuno-regulation ofMycobacterium tuberculosis infection. The relative shift from Interleukin-2 towards Interferon gamma (Interferon gamma/Interleukin-2) is more discriminatory for active tuberculosis. Protein carbonyl and Malondialdehyde, as oxidative stress markers, characte...
Source: Indian Journal of Clinical Biochemistry - October 1, 2018 Category: Biochemistry Source Type: research

Diagnostic Utility of Cord Blood Thyroid Stimulating Hormone in Congenital Hypothyroidism in the Era of Expanded Newborn Screening
This study was to assess the diagnostic utility of cTSH  >  20 μIU/L for screening CH. Generation of new cTSH value was the main outcome measure, to increase specificity. Designed as a cross-sectional analytic study in the neonatal unit of teaching hospital, 1200 term neonates with birth weight ≥2500 g, with no perinatal complications were included. N ewborn cTSH assay was done by chemi-luminescence. All screen positive were followed up on day five, 14 and 28 of life, to rule in or out CH (true or false positive). Positive predictive value and specificity were calculated. Receiver operating cha...
Source: Indian Journal of Clinical Biochemistry - October 1, 2018 Category: Biochemistry Source Type: research

Correlation of Red Blood Cell Acetylcholinesterase Enzyme Activity with Various RBC Indices
AbstractCholinesterases belongs to class hydrolases. There are two types acetylcholinesterase and butyryl cholinesterase. Acetylcholinesterase present in nerve endings and also in the RBC membrane. It helps to maintain the shape and size of RBCs. Any change in shape and size of RBCs may affect the activity of Acetylcholinesterase. Thus this study aimed to estimate RBCs Acetylcholinesterase enzyme activity in various types of anemias and correlate the RBCs Acetylcholinesterase enzyme activity with various hematological indices such as Erythrocyte Sedimentation Rate (ESR), Mean Corpuscular Hemoglobin (MCH), Mean Corpuscular ...
Source: Indian Journal of Clinical Biochemistry - October 1, 2018 Category: Biochemistry Source Type: research

Investigation of Trace Elements in the Hair and Nail of Patients with Stomach Cancer
This study analyzed some trace elements level in hair and nail of patients with stomach cancer, and compared with their level in healthy controls. Trace elements (Cu, Fe, K, Li, Mg, Mn, Na, P, Se, Sr and Zn) are estimated in hair and nail of the 73 cancer patients and 83 controls by atomic absorption spectrophotometric method. The levels of Cu, K, Li, P and Se in hair and nail samples, were significantly higher in cases than controls. Levels of Mg and Sr were significantly lower in cases than controls. Fe level in hair samples was significantly higher in cases than controls. The mean concentrations of Fe, Se and P signific...
Source: Indian Journal of Clinical Biochemistry - October 1, 2018 Category: Biochemistry Source Type: research

Role of HAMP Genetic Variants on Pathophysiology of Iron Deficiency Anemia
AbstractHepcidin is a 25-amino acid peptide hormone produced by hepatocytes and plays a key role in body iron metabolism. Hepcidin deficiency is the cause of iron overload in hereditary hemochromatosis, iron-loading anemia, and its excess is associated with anemia of inflammation, chronic disease and iron deficiency anemia (IDA). The aims of this study was to evaluateHAMP gene mutation, namely IVS2  + 1(–G) (c.148–150 + 1del) and Gly71 Asp (c.212G >  A (rs104894696) association with iron status in IDA conditions. Our study participants were 500 IDA patients and 550 age and sex-m...
Source: Indian Journal of Clinical Biochemistry - October 1, 2018 Category: Biochemistry Source Type: research

3,3 ′-Diindolylmethane Encapsulated Chitosan Nanoparticles Accelerates Inflammatory Markers, ER/PR, Glycoprotein and Mast Cells Population During Chemical Carcinogen Induced Mammary Cancer in Rats
AbstractThe present study aimed to investigate the effect of 3,3 ′-diindolylmethane (DIM) on inflammatory markers, estrogen receptors (ER), progesterone receptors (PR), level of glycoprotein and the mast cell population in 7,12-dimethylbenz (a) anthracene (DMBA) 25 mg/kg b.wt. induced rat mammary carcinogenesis. After 8 weeks of tumor formation, rats had acce ss to an oral administrated with DIM 10 mg/kg b.wt. and DIM@CS-NP 0.5 mg/kg body weight respectively for 8 weeks. The oral administration of DIM@CS-NP 0.5 mg/kg b.wt. suppressed the Cox-2, NF-κB and TNF-α protein express...
Source: Indian Journal of Clinical Biochemistry - October 1, 2018 Category: Biochemistry Source Type: research

Neurochemical and Behavioral Dysfunctions in Pesticide Exposed Farm Workers: A Clinical Outcome
AbstractThe problem of pesticides is not new and its exposure to human due to indiscriminate use is largely associated with the health related problems including neurotoxicological alterations. High levels of pesticide residues and their metabolites in the dietary constituents, food materials, maternal blood, cord blood, placenta breast milk have been reported and linked to alterations in birth weight, crown heel length, head circumference, mid-arm circumference and ponderal index of the neonates. Epidemiological studies have suggested that exposure of pesticide to human could be a significant risk factor for neurological ...
Source: Indian Journal of Clinical Biochemistry - September 22, 2018 Category: Biochemistry Source Type: research

Adaptive Neuro-Fuzzy Inference System-Based Exploration of the Interrelationships of 25-Hydroxyvitamin D, Calcium, Phosphorus with Parathyroid Hormone Production
AbstractThe rationale of the current study was to assess the prevalence of 25-hydroxyvitamin D (25-OHD) deficiency and hyperparathyroidism in South Indian population and to explore interrelationships of 25-OHD, Ca, P towards parathyroid hormone (PTH) production using adaptive neuro-fuzzy inference system (ANFIS). A total of 407 subjects (228 men 179 women) with the mean age 56.8  ± 14.1 were tested for these parameters. In view of the skewed distribution of biochemical variables, data segregation was performed in tertiles and this data was trained to generate fuzzy interference system based on subclust...
Source: Indian Journal of Clinical Biochemistry - September 20, 2018 Category: Biochemistry Source Type: research

Glutathione S-Transferase P1 313 (A   & gt;  G) Ile105Val Polymorphism Contributes to Cancer Susceptibility in Indian Population: A Meta-analysis of 39 Case–Control Studies
AbstractGSTP1 involved in the metabolism of carcinogens and toxins, reduces damage of DNA and act as a suppressor of carcinogenesis. Many studies have reported that 313 A  >  G polymorphism is associated with different cancer in Indian population, but the results remain conflicting rather than conclusive. Therefore, we have performed meta-analysis to clarify the more precise association ofGSPT1 313 A  >  G polymorphism with cancer risk in Indian population. We retrieved all relevant published literature from PubMed (Medline) and Google scholar web database and included those study only bas...
Source: Indian Journal of Clinical Biochemistry - September 17, 2018 Category: Biochemistry Source Type: research

Empagliflozin Attenuates Renal and Urinary Markers of Tubular Epithelial Cell Injury in Streptozotocin-induced Diabetic Rats
AbstractEmpagliflozin, a SGLT-2 inhibitor, improves diabetic nephropathy through its pleiotropic anti-inflammatory effects. The present study aims to evaluate empagliflozin effects on renal and urinary levels of tubular epithelial cell injury markers in streptozotocin-induced diabetic rats. Empagliflozin at 10  mg/kg (p.o.) was administered for 4 weeks, beginning 8 weeks after induction of diabetes. Renal function as well as markers of renal tubular epithelial cell injury were assessed in kidney tissue homogenates and urine. Empagliflozin was able to ameliorate diabetes induced elevations in serum cyst atin ...
Source: Indian Journal of Clinical Biochemistry - September 17, 2018 Category: Biochemistry Source Type: research

Morphometric Evaluation of the Seminiferous Tubules and the Antioxidant Protective Effects of Gallic Acid and Quercetin in the Testis and Liver of Butyl Phthalate Treated Rats
AbstractThe antioxidant protective effects of gallic acid (GAL) and quercetin (QUE) against oxidative stress induced by di-butyl phthalate (DnBP) in the liver and testis of rats were evaluated in this study. Adult albino Wistar rats (180 –225 g) were treated with QUE or GAL (50 mg/kg) alone or in combination with DnBP (1 mL/kg) for 15 days. After treatment, tissue samples were taken for determination of glutathione and malondialdehyde levels, and superoxide dismutase and catalase activities. Serial sections of the testis and l iver were stained with haematoxylin and eosin for microscopy and semini...
Source: Indian Journal of Clinical Biochemistry - September 15, 2018 Category: Biochemistry Source Type: research

Protective Role of Black Tea Flavonoids Against Ethanol-Induced Gastropathy via Matrix Metalloproteinase Pathway
AbstractTea polyphenols are known to prevent various ailments like cancer, atherosclerosis, hypertension and diabetes. Our study aimed at to decipher the gastroprotective effect of aqueous black tea extract (BTE) against ethanol-induced gastric damage and the role of BTE in modulating MMP-9 activity and expression, both in vivo and in vitro. The protective role of BTE was assessed in Sprague –Dawley rats after inducing damage with 70% ethanol. Human gastric adenocarcinoma cells (AGS) were treated with ethanol in ex vivo experiment. MMP-9 activity and expression were investigated through gelatin zymography and western...
Source: Indian Journal of Clinical Biochemistry - August 23, 2018 Category: Biochemistry Source Type: research

The Comparison of Iodine-Type and MnO 2 -Type Oxidation for Measuring the Levels of Urine Neopterin and Biopterin in Patients with Hyperphenylalaninemia: A Descriptive-Analytic Study in Iran
AbstractFresh urine sample is required for measurement of pterins in patients with hyperphenylalaninemia (HPA) for detection of tetrahydrobiopterin (BH4) coenzyme deficiency. The present study aimed to measure the levels of neopterin and biopterin in the urine of patients with HPA using manganese dioxide (MnO2) and iodine oxidation methods. The levels of neopterin and biopterin in the urine of 124 patients were measured using two methods of oxidation (MnO2 and iodine) and the results were statistically analyzed using correlation test, Roc-Curve and ANOVA in SPSS-20 software. The level of significant was determined to beP&t...
Source: Indian Journal of Clinical Biochemistry - August 23, 2018 Category: Biochemistry Source Type: research

Serum Biomarker Based Algorithms in Diagnosis of Ovarian Cancer: A Review
AbstractEpithelial ovarian cancer accounts for more than 90% of ovarian tumours and continues as a leading cause of death from gynaecological malignancies. It is often difficult to differentiate a benign ovarian mass from malignant ones. Invasive histopathological biopsy is used as the gold standard diagnostic tool to diagnose cancer in patients with ovarian mass. A wide spectrum of Biomarkers were tried in various studies to develop a non invasive diagnostic tool, out of which HE4 and CA 125 remain the only clinically useful biomarker. Consequently various Biomarker based algorithms i.e. Risk of Malignancy Index, risk of ...
Source: Indian Journal of Clinical Biochemistry - August 6, 2018 Category: Biochemistry Source Type: research

Association Between Serum Testosterone and Serum PSA Among Men With and Without Partial Androgen Deficiency
AbstractSerum prostatic specific antigen (PSA) and serum testosterone levels share an undefined relationship with each other, with many conflicting studies showing both positive and negative correlation between them. Our aim was to assess association between serum PSA and serum testosterone in healthy men with normal testosterone levels and men with partial androgen deficiency (PADAM). A cross sectional study was conducted at a teaching hospital setting where serum testosterone and aging male symptom scale  (AMS) scores along with PSA were studied in 255 men (>  50 years) with and without PADAM. Mean to...
Source: Indian Journal of Clinical Biochemistry - July 31, 2018 Category: Biochemistry Source Type: research

Genotyping and Frequency of PCSK9 Variations Among Hypercholesterolemic and Diabetic Subjects
Abstract Non-synonymous single-nucleotide polymorphism (SNPs) in the gene for proprotein convertase subtilisin/kexin type 9 (PCSK9) can influence cholesterol and glucose metabolism, leading to increased risk of cardiovascular disease and diabetes. To determine the frequency of four common PCSK9 SNPs, L10Ins, A56V, I474V, and E670G, in a population sample (n  = 98) of the Hail region of Kingdom of Saudi Arabia. Blood was collected from participants; serum cholesterol, blood glucose and glycated hemoglobin were determined; genomic DNA was extracted and PCR amplicons from SNP-containingPCSK9 exons were subject...
Source: Indian Journal of Clinical Biochemistry - July 30, 2018 Category: Biochemistry Source Type: research

A Prognostic and Predictive Study of BCR-ABL Expression Based on Characterization of Fusion Transcripts
This study comprises BCR-ABL expression in correlation with age, gender and trans cript type and disease monitoring in follow-up samples. Eighty-seven chronic phase CML patients were enrolled in the study. Out of these 24 patients were followed further. Quantitative Real time PCR was performed to assess the BCR-ABL expression followed by gel electrophoresis of PCR products. The r esults were expressed as CN/µg RNA. The results obtained were correlated with SPSS 16.0 software. We found three different types of the expression that includes b2a2, b3a2 and co-expression (b2a2 + b3a2). Higher incidence of b2...
Source: Indian Journal of Clinical Biochemistry - July 28, 2018 Category: Biochemistry Source Type: research

Pre-analytical Errors in Glucose Estimation Results in Query on Diabetic Management
Abstract Status of diabetes of an individual is majorly evaluated by the frequent monitoring of glucose estimation. Use of serum samples and inappropriate plasma for estimating glucose is an existing practise in Indian standard of laboratories. There is a strong evidence for  occurrence of in vitro glycolysis on the above mentioned specimens. The aim is to study the pre-analytical variations on the glucose estimation of using sodium fluoride–disodium EDTA (NaF–Na2EDTA) plasma (glycolysis inhibiting anticoagulant) and determine the fact behind the activity of glycol ysis inhibition on the same. Healthy volu...
Source: Indian Journal of Clinical Biochemistry - July 21, 2018 Category: Biochemistry Source Type: research

In Vivo Anti-diarrheal Activity of Methanolic Extract of Streblus asper Leaves Stimulating the Na + /K + -ATPase in Swiss Albino Rats
AbstractStreblus asper Lour., traditionally used for anti-diarrheal effects as like dysentery and diarrhea. The present study aims to prove the anti-diarrheal activities of methanolic extract of leaves ofS. asper in animal models. The anti-diarrheal activity was evaluated using castor oil-induced diarrhea and magnesium sulphate-induced diarrhea models whereas anti-motility activities were investigated using gastrointestinal transit test examined in animal models. In castor oil-induced diarrhea model, methanolic extract ofS. asper (MESA) at the doses of 100, 200, and 400  mg/kg produced statistically significant (P 
Source: Indian Journal of Clinical Biochemistry - July 21, 2018 Category: Biochemistry Source Type: research

Cystine Urolithiasis in Early Childhood
We present an infant with recurrent gross hematuria due to cystine urolithiasis for its rarity. (Source: Indian Journal of Clinical Biochemistry)
Source: Indian Journal of Clinical Biochemistry - July 20, 2018 Category: Biochemistry Source Type: research

Effects of Cysteine-Stabilised Peptide Fraction of Aqueous Extract of Morinda lucida Leaf on Selected Cardiovascular Disease Indices in Mice
This study evaluated the effects of cysteine-stabilised peptide fraction (CSPF) of aqueous extract ofMorinda lucida leaf on selected cardiovascular disease indices in mice. Sixty adult Swiss Albino mice were randomly divided into 6 groups (n  = 10). Group A served as control and received 5% DMSO. Half of the mice in groups B, C, D, E and F received 31.25, 62.5, 125, 250, and 500 mg/kg body weight of CSPF respectively for 7 days while the other half received the various doses for 28 days. After the experimental period, selected ca rdiovascular disease indices were determined in the mice. The res...
Source: Indian Journal of Clinical Biochemistry - July 17, 2018 Category: Biochemistry Source Type: research

New Biomarkers as Prognostic Factors for Cardiovascular Complications in Type 2 Diabetic Patients
This study was initiated to explore some novel biomarkers like pro-inflammatory markers (chemerin and visfatin) and anti-inflammatory marker (omentin-1) as prognostic factors for cardiovascular complications in type 2 diabetic patients. Forty diabetic patients without cardiovascular disease, 40 diabetic patients with cardiovascular disease and twenty healthy control counterparts were included in this study. Serum chemerin, omentin-1 and visfatin levels were quantified. Receiver operating characteristic curve analysis was done to identify the cut off value for each marker. The mean serum level of chemerin was 57.65  ...
Source: Indian Journal of Clinical Biochemistry - July 17, 2018 Category: Biochemistry Source Type: research

Assessment of FTO Gene Polymorphism and its Association with Type 2 Diabetes Mellitus in North Indian Populations
This study was done, due to a continuous increase in the risk of obesity and type 2 diabetes in north Indian population, because of lifestyle and genetic variations. Clinically diagnosed subjects of type 2 diabetes mellitus (as per ADA criteria) were taken as cases and age and sex matched subjects without any associated illness were taken as controls. Obesity was estimated by calculating waist circumference and BMI in the study cases and controls. For genetic variation, DNA was isolated with Quaigen kit method and isolated DNA was amplified with PCR. Amplified DNA was resolved in 1% agarose gel electrophoresis. The Hardy &...
Source: Indian Journal of Clinical Biochemistry - July 16, 2018 Category: Biochemistry Source Type: research

Association of Vitamin D and Parathyroid Hormone Levels in Overweight and Obese Adolescents
AbstractVitamin D deficiency in known to be high in obese and overweight adolescents. Few studies in other countries have shown lower parathyroid hormone (PTH) levels in obese adolescents despite high vitamin D deficiency. The aim of the study is to assess vitamin D and PTH levels and their relationship in overweight and obese adolescents in Telangana. This cross-sectional study was carried out in 108 overweight and obese boys and girls aged 12 –18 years. A pretested Questionnaire was administered to collect information on demographics, sun exposure, medical and nutritional history. Anthropometry and blood sampl...
Source: Indian Journal of Clinical Biochemistry - July 11, 2018 Category: Biochemistry Source Type: research

Phosphorus Compounds: Their Discovery in Biological World
(Source: Indian Journal of Clinical Biochemistry)
Source: Indian Journal of Clinical Biochemistry - June 28, 2018 Category: Biochemistry Source Type: research

CCR5, MCP-1 and VDR Gene Polymorphisms Are Associated with the Susceptibility to HBV Infection
The objective of the study, was to evaluate the association between theCCR5D32,CCR5-2459A/G,MCP-1-2518A/G,VDR-APa1A/C,VDR-Taq1T/C SNPs and HBV susceptibility, in samples of Iranian populations. TheCCR5D32,CCR5-2459A/G,MCP1-2518A/G,VDR-APa1A/C,VDR-Taq1T/C polymorphisms were analyzed by polymerase chain reaction and PCR-RFLP using 100 chronic HBV infected (HBV) patients, 40 spontaneously recovered HBV (SR) subjects and 100 healthy controls (C). Also, serum levels of protein were monitored. The study showed that the existence ofCCR5-2459A,MCP1-2518G andVDR-CC alleles significantly increased risk of chronic HBV infection. In a...
Source: Indian Journal of Clinical Biochemistry - June 27, 2018 Category: Biochemistry Source Type: research

Study of Insulin-Loaded Chitosan Nanoparticle Effects on TGF- β1 and Fibronectin Expression in Kidney Tissue of Type 1 Diabetic Rats
AbstractIn diabetes, the increasing blood glucose levels through oxidative stress, with increase in inflammatory cytokines and growth factors, such as TGF- β1, can cause long-term complications, including nephropathy. Subcutaneous injection of insulin is a common method used to treat Type 1 diabetes, which can lead to problems such as hypoglycemia and edema. In the present study, we examined the effect of insulin in its two injectable and oral forms o n the expression of TGF-β1 and fibronectin in kidney tissue of STZ diabetic rats. A total of 25 male Wistar rats were randomly divided into 5 groups: C: normal cont...
Source: Indian Journal of Clinical Biochemistry - June 26, 2018 Category: Biochemistry Source Type: research

Evaluating NISCH and CDH1 Promoter Hypermethylation in Nonsmokers, Cancer Free Smokers and Lung Cancer Patients: A Case Control Study
AbstractLung cancer has very high mortality due to late stage diagnosis not amenable to curative resection. Cancer specific methylation patterns of tumor suppressor genes may precede precursor lesions of lung cancer. Our aim was to evaluate the promoter hypermethylation of tumor suppressor gene NISCH and CDH1 in cfDNA from plasma of lung cancer patients and its possible correlation with smoking status and various clinicopathological parameters. Forty histopathologically confirmed lung cancer cases, thirty smoker and thirty nonsmoker controls were enrolled. Plasma cfDNA was extracted and subjected to bisulfite treatment fol...
Source: Indian Journal of Clinical Biochemistry - June 23, 2018 Category: Biochemistry Source Type: research

Diabetic Nephropathy Assessment: Microtubule-Associated Protein 1 Light-Chain 3B a New Promising Biomarker
AbstractAutophagy is a highly conserved pathway. Impairment of autophagy is implicated in the pathogenesis of diabetic nephropathy. The current study applied a bioinformatics analysis to retrieve promising autophagy biomarker relevant diabetic nephropathy. Urinary expression of Microtubule-associated protein 1 light-chain 3B (LC3B) RNA was assessed. Urine samples of 86 type II diabetic kidney disease Egyptian patients (albuminuria group) were provided to quantify urinary expression of LC3B. A group of 30 healthy volunteers were also enrolled in addition to non-albuminuria group including 44 patients. Our study revealed a c...
Source: Indian Journal of Clinical Biochemistry - June 22, 2018 Category: Biochemistry Source Type: research

TP53 rs1625895 is Related to Breast Cancer Incidence and Early Death in Iranian Population
In this study, we have investigated the role of rs1625895 polymorphism in breast cancer incidence in Iranian women. DNA extraction of 86 breast cancer patients and 96 control individuals have been used for allele-specific primer-PCR and genotyping of allele A and allele G of theTP53 rs1625895. Genotypes frequencies have been shown that GG homozygosis as the most frequent genotype is a significant association with increased risk of breast cancer development in Iranian women (odds ratio  = 6,p  =  0.002). On the other hand and in comparison to allele G, allele A could cause early death of breast c...
Source: Indian Journal of Clinical Biochemistry - June 21, 2018 Category: Biochemistry Source Type: research

β-Naphthyl Acetate in Acetone Produces a Dark Background for Staining of Esterase Activity on Gel
(Source: Indian Journal of Clinical Biochemistry)
Source: Indian Journal of Clinical Biochemistry - June 21, 2018 Category: Biochemistry Source Type: research

First Report of Association Between Rare α-Thalassemia Mutation ( HBA1 : c.298A & gt;T) and Hb Fontainebleau ( HBA2 : c.64G & gt;C)
Abstractα-Thalassemia is one of the most common monogenic diseases worldwide. The combination of alpha-chain variants with thalassemia mutations may lead to clinical and hematological characteristics, which is of importance for genetic counseling. The present study describes for the first time a rare α1-g lobin nonsense mutation, codon 99 (HBA1: c.298A>T) associated with a α2-chain variant Hb Fontainebleau (HBA2: c.64G>C) in a family from northern Iran. The case is a 23-year-old man with hypochromic microcytic anemia that requested for prenatal diagnosis. The combination of α1-globin mutation ...
Source: Indian Journal of Clinical Biochemistry - June 18, 2018 Category: Biochemistry Source Type: research

Comparison of Serum Thyroglobulin Levels in Differentiated Thyroid Cancer Patients Using In-House Developed Radioimmunoassay and Immunoradiometric Procedures
AbstractThyroglobulin (Tg) is a proven tumor marker in the follow-up and post-operative management of patients with differentiated thyroid cancer (DTC). All assays for serum thyroglobulin (s-Tg) are based on immunoassays, however, the assay technique has a bearing on the variations seen in the estimations. We studied this using four in-house developed radioimmunoassays (RIA) and immunoradiometric assays (IRMA). Limit of detection, working range, recovery, dilution test, precision profiles and method comparison were evaluated. All four methods were used for the estimation of s-Tg in DTC patients and also compared for their ...
Source: Indian Journal of Clinical Biochemistry - June 18, 2018 Category: Biochemistry Source Type: research

Heterophile Antibody to Adrenocorticotropin Hormone Interfering with the Investigation of Cushing ’s Syndrome
AbstractThe practice of medicine depends on the accuracy of biochemical assays. The high prevalence of incidental masses on imaging necessitates a correct biochemical diagnosis before proceeding to radiological studies. Hormonal assays, tumour markers, and markers of cardiac injury are particularly susceptible to heterophile antibody interference which may lead to inaccurate and misleading results, inappropriate investigation and/or treatment, patient concern and potential harm. A case of heterophile antibody interference in the measurement of ACTH in a patient with Cushing ’s syndrome resulting in unnecessary invasi...
Source: Indian Journal of Clinical Biochemistry - June 16, 2018 Category: Biochemistry Source Type: research