Indian Journal of Clinical Biochemistry This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Evaluation of Proflo-U ® Platform: A Novel Fluorescence-Based Point of Care Device for Quantitative Estimation of Urine Albumin
This study compares Proflo-U ® a novel PoC device that estimates urine albumin, with standard immunoturbidity based assays. Proflo-U® is a novel PoC device which works with an android based mobile application by Bluetooth connectivity. This unique fluorescence-based urine albumin assay has been compared with the well-establis hed gold standard Immunoturbidity based method on Beckman Coulter AU platform and also using Biosystem kit. The Proflo-U® generated data from standard recombinant human serum albumin (rHSA) and human urine spiked with rHSA has shown high correlationR2 ~ 0.99 andp >  0.05. This novel plat...
Source: Indian Journal of Clinical Biochemistry - February 25, 2024 Category: Biochemistry Source Type: research

Genetic Variants of AGO1*rs595961 and AGO2*rs4961280 with Susceptibility to Bladder Carcinoma
This study aimed to investigate the relationship betweenAGO1 and AGO2 gene variants with the elevated risk of bladder carcinoma (BLCA) using genetic, biochemical, and bioinformatic approaches. This retrospective study comprised of 252 participants [122 BLCA patients and 130 cancer-free controls], matched for age and gender. TheAGO1*rs595961 and AGO2*rs4961280 genetic variants were characterized using TaqMan genotyping assay. A bioinformatics framework, stratification analysis, and multivariate regression were employed. The genetic distribution ofAGO1*rs595961 was associated with an increased risk of bladder carcinoma under...
Source: Indian Journal of Clinical Biochemistry - February 24, 2024 Category: Biochemistry Source Type: research

Exploring the Role of Serum Lysyl Oxidase (LOX) Levels and LOX Gene Polymorphism (G473A) in Diabetic Nephropathy
AbstractDiabetic Nephropathy (DN) is a leading microvascular complication of diabetes with poor prognosis. Extra cellular matrix accumulation (ECM) leading to renal fibrosis is the key manifestation of diabetic kidney. Lysyl oxidase (LOX) plays an important role in cross linking of collagen. Altered LOX expression and LOX depended cross links with Diabetes have been documented both in rat and human model. Evidence of LOX gene polymorphism (G473A) has been shown limited to Diabetic foot ulcer. Studies on association of single nucleotide polymorphism (SNP) (G473A) of LOX gene with Diabetic nephropathy is scarce. A pilot stud...
Source: Indian Journal of Clinical Biochemistry - February 20, 2024 Category: Biochemistry Source Type: research

The First-Ever Investigation of SNP rs119461977 in SECISBP2/SBP2 Gene and its Implications for Hypothyroidism: A Novel Case –Control Research
This study indicates the novel association of rs119461977 of the SBP2 gene with hypothyroidism. This association underlines the importance of selenoproteins synthesis in the pathogenesis of hypothyroidism. (Source: Indian Journal of Clinical Biochemistry)
Source: Indian Journal of Clinical Biochemistry - February 20, 2024 Category: Biochemistry Source Type: research

Spirulina Platensis Alleviates γ- Radiation-Induced Brain Damage by Attenuating Oxidative Stress and Increasing Brain-Derived Neurotrophic Factor (BDNF) Levels in Male Albino Rats
AbstractIonizing radiation  can irreversibly damage the brain cells’ function and structure.Spirulina platensis (Sp) is widely employed as a dietary supplement because it has anti-inflammatory, antioxidant, and immunomodulating properties. Therefore, this work aims to investigate the protective properties of Sp towards γ-irradiation (IRR)-induced brain damage. The whole-body male albino rats were exposed to IRR with a 7 Gy single dose in the absence or presence of Sp (400 mg/kg body weight). The rats were divided into four groups: Group I (control), Group II (Sp), Group III (IRR), and Group IV (Sp + IRR +  Sp...
Source: Indian Journal of Clinical Biochemistry - February 17, 2024 Category: Biochemistry Source Type: research

Role of Blood Cell Indexes in Progresses to ESRD
This study aimed to explore the predictive potential of specific blood cell indexes in forecasting further renal function decline and the transition from CKD stage 3 –4 to ESRD. We assessed the following blood cell indexes in 377 CKD stage 3–4 patients: absolute neutrophil count (ANC), neutrophil/lymphocyte ratio (NLR), platelet/lymphocyte ratio (PLR), derived NLR (dNLR), mean platelet volume (MPV), aggregate index of systemic inflammation (AISI), and system ic inflammation index (SII). ANC, MPV, NLR, PLR, dNLR, and SII were found to independently predict a rapid decline in eGFR. Notably, NLR and dNLR demonstrated the ...
Source: Indian Journal of Clinical Biochemistry - February 13, 2024 Category: Biochemistry Source Type: research

Metabolomic Biomarkers for Prognosis in Non-Muscle Invasive Bladder Cancer: A Comprehensive Systematic Review and Meta-Analysis
AbstractThe prognostic biomarkers, or metabolites, have gained relevance due to their significance in predicting clinical and therapeutic outcomes and guiding informed therapy options. This systematic review and meta-analysis aimed to evaluate the prognostic significance of metabolites in non-muscle-invasive bladder cancer (NMIBC) through an array of literature. The PubMed, Web of Science, Embase, and Cochrane Library databases were comprehensively searched for eligible studies published between January 2010 and August 2022, using related keywords and  MeSH terms. Two reviewers performed the extraction process, and a thi...
Source: Indian Journal of Clinical Biochemistry - February 9, 2024 Category: Biochemistry Source Type: research

Role of Cell-free DNA as a Non-Invasive Biomarker in the Detection of Head and Neck Squamous Cell Carcinoma
AbstractHead and neck squamous cell carcinomas (HNSCC) are the sixth leading cancer by incidence worldwide. Small fragments of cell-free DNA (cfDNA) are present in the circulation with elevated levels in cancer patients. Their concentration correlates with tumor size, disease stage, and metastatic burden. Our present study aims to determine the utility of cfDNA as an early screening and diagnostic tool in patients with HNSCC. A cross-sectional study was conducted. The cohort included 35 biopsy-confirmed cases of HNSCC and 35 age and sex-matched healthy controls. cfDNA was extracted using 3  ml plasma with QIAamp Circulati...
Source: Indian Journal of Clinical Biochemistry - February 5, 2024 Category: Biochemistry Source Type: research

Unveiling the Zinc Paradox: Balancing Act in Reproductive Health and Prostate Pathology
AbstractMinerals play a crucial role in the biochemical and physiological processes of all living organisms, including humans. Metals like Zinc, Copper, Calcium, and Selenium are essential for optimal functioning of these processes. Zinc is crucial for maintaining the endocrine system, particularly prostate tissue, and has been linked to prostate cancer and benign prostatic hyperplasia. Zinc levels and ratios have been found to be decreased in prostate carcinoma cases compared to benign prostatic hyperplasia cases. This letter to the editor supports a recent finding in the Indian Journal of Clinical Biochemistry, which con...
Source: Indian Journal of Clinical Biochemistry - February 5, 2024 Category: Biochemistry Source Type: research

A New Machine Learning Approach for Actual Calcium Measurement
AbstractIonized calcium is a better measure but still many healthcare setups use total calcium to determine the calcium status due to wider availability on major clinical chemistry automated platforms. The performance of calcium correction formulas varies in different populations, limiting their wider use. Machine learning models can study multiple variables with their complex interactions, hence predict actual calcium more accurately. The present study evaluated the performance of Random forest and XGBoost machine learning models and compared it with the conventional corrected calcium calculation formulas, in eastern Indi...
Source: Indian Journal of Clinical Biochemistry - February 2, 2024 Category: Biochemistry Source Type: research

Circulating miRNA-373 and Vascular Endothelial Growth Factor as Potential Biomarkers for Early Detection of Breast Cancer
AbstractBreast cancer is the leading cause of cancer-related mortality among women worldwide. MicroRNAs (miRNAs), short non-coding RNAs, have been implicated in cancer-related processes such as tumor development, metastasis, angiogenesis, and drug resistance. Circulating miRNA-373 demonstrates higher relative exosomal serum levels in breast cancer patients compared to healthy women, making it a potential non-invasive biomarker. Separately, vascular endothelial growth factor (VEGF) is crucial for angiogenesis, and is elevated in breast cancer. In this case –control study, we aimed to investigate the diagnostic accuracy of...
Source: Indian Journal of Clinical Biochemistry - February 2, 2024 Category: Biochemistry Source Type: research

Genomic DNA Extraction from Different Human Tissue Sample: Urine, Oral Gargle, Blood, and Cervical for Real Time Amplification by qPCR
AbstractMolecular technologies have been a driver of rapid paradigm shift in the field of scientific research and clinical applications. Adequate and pure form of nucleic acid extracted from efficient biological sources together with an easy, simple, standardized protocol, ease of access and acceptability is also a prerequisite for genotyping analysis for different disorders. Urine and oral gargle samples are emerging as a potential source of genomic DNA (gDNA) and may offer a better alternative to existing sources such as blood. This manuscript compares for the first time, the quality and quantity of gDNA extracted manual...
Source: Indian Journal of Clinical Biochemistry - January 26, 2024 Category: Biochemistry Source Type: research

The Role of Digital Biomarkers in Monitoring the Health Parameters in Individuals with Obesity
(Source: Indian Journal of Clinical Biochemistry)
Source: Indian Journal of Clinical Biochemistry - January 24, 2024 Category: Biochemistry Source Type: research

Exon Sequence Analysis of the ATG5, ATG12, ATG9B Genes in Colorectal Cancer Patients During Radiotherapy
AbstractRadiotherapy (RT) which is a treatment regime for cancer patients may cause genetic instability and side effects. Etiological associations exist amongst autophagy-related gene (ATG) mutation and cancer. RT increases the rate of autophagy previously proven in vitro. The aforementioned background diverted us to conduct exon mutation analysis for ATG5, ATG12, and ATG9B genes of colorectal cancer patients who were receiving neoadjuvant RT. Peripheral blood DNA from different time points (before/middle/after RT) of the same patients was isolated and most tandem repeat-containing exons of ATG5, ATG12, and ATG9B were poly...
Source: Indian Journal of Clinical Biochemistry - January 18, 2024 Category: Biochemistry Source Type: research

Serum Biomarkers and Gene Polymorphisms in COPD and COPD with T2DM Patients
AbstractInflammation plays an important role in chronic obstructive pulmonary disease (COPD). Increasing evidence points to the role of inflammation in the pathogenesis of type 2 diabetes  mellitus (T2DM). We investigated the adiponectin: leptin and IL-8 genes in COPD-induced T2DM patients, along with the effect of polymorphism on the levels of these cytokines. We enrolled COPD, COPD plus T2DM, and healthy subjects from the institution according to standard criteria. Further, we dre w a blood sample and performed genotypic analysis and an enzyme-linked immunosorbent assay (ELISA). We enrolled a total of 500 subjects in th...
Source: Indian Journal of Clinical Biochemistry - January 17, 2024 Category: Biochemistry Source Type: research