Indian Journal of Clinical Biochemistry This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

COVID-19 and Gut Microbiota: A Potential Connection
AbstractCurrently, world is facing a global outbreak causing a pandemic threat known as COVID-19. This infectious disease is triggered by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). Gut microbiota harbours multi species community with a strong impact on host immune homeostasis. However, our knowledge about this gut microbiota and its symbiotic relationship with immune activation in association with SARS-CoV-2 is limited. Unbalanced bacterial flora with too many opportunistic infections can shift immune system towards a cascade of inflammatory responses leading to multi organ damage. This review will highl...
Source: Indian Journal of Clinical Biochemistry - January 21, 2021 Category: Biochemistry Source Type: research

Incidental Detection of Hemoglobin Variants During Evaluation of HbA1c
AbstractHemoglobinA1c (HbA1c) is used to diagnose Diabetes mellitus and monitor glycemic control over the previous eight to twelve weeks in diabetic patients. Detection of HbA1c by cation exchange-high performance liquid chromatography (CE-HPLC) gives a chromatogram by which abnormal hemoglobin variants are also picked up. Some of these may interfere with HbA1c values affecting clinical management. Due to increased inter-state migration as well as medical tourism, there is a high possibility of finding various hemoglobin variants in any part of India. We did a prospective analysis over 1.5  years, of the hemoglobin varian...
Source: Indian Journal of Clinical Biochemistry - January 21, 2021 Category: Biochemistry Source Type: research

Direct Relationship Between Uric Acid and C-Reactive Protein and Its Implication in the Chronic Kidney Disease
In this study, serum uric acid, creatinine, urea and C-Reactive protein levels were significant raised in CKD group to that of controls. Notably serum C-Reactive protein levels were significantly higher in the CKD patients associated with non-diabetic factors in comparison to diabetic associated CKD patients. A receiver operating characteristic curve (ROC) exhibited a strong association between uric acid and C-Reactive protein levels. A strong direct relationship between serum uric acid and C-Reactive protein in CKD patients are plausibly culprits in the progression of diseases. (Source: Indian Journal of Clinical Biochemistry)
Source: Indian Journal of Clinical Biochemistry - January 20, 2021 Category: Biochemistry Source Type: research

Correlation of Serum Retinol and Atherogenic Indices in Type 2 Diabetes Mellitus: A Case –Control Study
AbstractDyslipidemia is an important risk factor for atherosclerosis and coronary heart disease, leading to mortality and morbidity in subjects with T2DM. This risk is higher in subjects with diabetes who are on retinoid therapy for some other indication, where hypercholesterolemia, hypertriglyceridemia, and low serum high-density lipoprotein cholesterol (HDL-C), and sudden cardiovascular deaths have been reported. Our study aimed to find the correlation of serum retinol and atherogenic index (AI) in subjects with T2DM and compare them with healthy controls. We found there was a significant difference in systolic and diast...
Source: Indian Journal of Clinical Biochemistry - January 20, 2021 Category: Biochemistry Source Type: research

A Rare Case of Dysmorphism with Duplication in Chromosome 22
(Source: Indian Journal of Clinical Biochemistry)
Source: Indian Journal of Clinical Biochemistry - January 20, 2021 Category: Biochemistry Source Type: research

Discordant Hemoglobin Values Determined by Blood Gas Analyzer and Hematology Analyzer Synchronously Is a Warning for Inappropriate Blood Sampling
AbstractA patient may have two contemporary hemoglobin test results; one received from a hematology analyzer and the other from a blood gas analyzer. Such results may pose a dilemma if validated without investigation. A 27-year-old female patient presented to the emergency department because of worsening dyspnea and hemoptysis. Her hemogram revealed Hb 4.5  g/dL; Hct 16.1% while blood gas analysis showed Hb 8.1 g/dL; Hct 25.3%. The results were evaluated to be suspicious. All results were rejected and new requests were made. Hb 9.8 g/dL; Hct 31.9% were seen in repeated hemogram while blood gas analysis showed Hb 10.2 g...
Source: Indian Journal of Clinical Biochemistry - January 16, 2021 Category: Biochemistry Source Type: research

Investigating Investigation (Ab)Use: Thyroid Function Test Audit in a Tertiary Care Teaching Institute in Eastern India
This study was conducted to study the investigation requesting pattern of TFTs. Our results showed that TFT panel was ordered in almost equal numbers (35.58%) as single test of TSH (41.27%). Subclinical thyroid disease was diagnosed in 22.1% of cases and the rest were excluded as having any thyroid dysfunction. Over 2/3rd of all requests were for women. An important conclusion from our study was that, the essentiality of lab tests is a decision entirely in the hands of the treating physician keeping in mind the cost and best outcome for patients. Hospitals can develop strategic protocols for ordering laboratory tests keepi...
Source: Indian Journal of Clinical Biochemistry - January 15, 2021 Category: Biochemistry Source Type: research

Placental Expression and Relative Role of Anti-inflammatory Annexin A1 and Animal Lectin Galectin-3 in the Pathogenesis of Preeclampsia
AbstractPreeclampsia (PE) remains the major cause for maternal and foetal mortality and morbidity all over the world. Preeclampsia is associated with maternal, placental aggravated inflammatory response and generalized endothelial damage. AnnexinA1 (AnxA1) is glucocorticoid regulated protein regulates a wide range of cellular and molecular steps of the inflammatory response and is implicated in resolution of inflammation. Galectin-3 (Gal-3), β-galcotoside-binding lectin participates in many functions, both intra- and extracellularly. Recently it has been shown that galectin-3 modulates the inflammation. Role of AnxA1 and ...
Source: Indian Journal of Clinical Biochemistry - January 12, 2021 Category: Biochemistry Source Type: research

Role of Regulatory Proteins Involved in Iron Homeostasis in Pulmonary Tuberculosis Patients and Their Household Contacts
AbstractEarly identification and treatment of active tuberculosis disease among high risk household contacts could limit new transmission and better clinical outcome, thus decreasing TB burden. Host iron homeostasis is an important yet underevaluated factor in pathophysiology of tuberculosis (TB). One such protein is hepcidin which internalizes ferroportin (membrane iron transporter), thus inhibiting iron export from macrophages which is utilised by bacteria leading to disease severity. Iron homeostasis markers were evaluated in 50 pulmonary tuberculosis patients (PTB) and their household contacts to assess their utility a...
Source: Indian Journal of Clinical Biochemistry - January 6, 2021 Category: Biochemistry Source Type: research

Analytical Method Validation for Estimation of Neurotransmitters (Biogenic Monoamines) from Cerebrospinal Fluid Using High Performance Liquid Chromatography
AbstractBiogenic amine neurotransmitters such as serotonin and dopamine are essential for signaling in both central and peripheral nervous system.  Their metabolism is a multistep pathway and any defect in this results in alteration in metabolites of serotonin 5-Hydroxyindole acetic acid (5HIAA) and dopamine homovanillic acid (HVA) and 3-O-Methyl Dopa (3-OMD). Estimation of these metabolites in cerebrospinal fluid (CSF) assists in diagnosis of neurotransmitter defects. Their estimation is technically demanding and is currently available only in referral centers. We aimed to optimize a method for analysis of 5HIAA, HVA an...
Source: Indian Journal of Clinical Biochemistry - January 6, 2021 Category: Biochemistry Source Type: research

Development of Flow Injection Analysis Method for the Second-Tier Estimation of Succinylacetone in Dried Blood Spot of Newborn Screening
AbstractTyrosinemia type 1 (TYR1) is a devastating aminoacidopathy, leading to mortality without medical intervention. Although, detection and quantification of tyrosine in dried blood spot (DBS) is possible, but being a non-specific marker for TYR1 and its frequent association with transient neonatal tyrosinemia limits its applicability. Despite, Succinylacetone (SUAC) being a pathognomonic marker for TYR1, but not often detectable by routine newborn screening (NBS). We envisaged to determine SUAC in DBS by an in-house flow injection analysis method on a liquid chromatography/tandem mass spectrometry (LC –MS/MS). Succin...
Source: Indian Journal of Clinical Biochemistry - January 6, 2021 Category: Biochemistry Source Type: research

One Year of COVID-19: The “New Normal”
(Source: Indian Journal of Clinical Biochemistry)
Source: Indian Journal of Clinical Biochemistry - January 5, 2021 Category: Biochemistry Source Type: research

Ameliorative Impact of Aloe vera on Cartap Mediated Toxicity in the Brain of Wistar Rats
AbstractExposure to pesticides can pose a greater threat to multiple organs of nontarget animals. Cartap is a thiocarbamate pesticide, broadly used in agricultural fields. The assessment of neurotoxicity of cartap has not been properly studied in the mammalian systems. The present investigation unveils the toxic effects of cartap in the brain of Wistar rats its amelioration by using aqueous extract ofAloe vera leaves. We have used 4 groups of animals comprising six in each: Group 1- control, Group 2- control withAloe vera, Group 3- cartap, Group 4- cartap withAloe vera treated. After 15  days of treatment, biochemical inv...
Source: Indian Journal of Clinical Biochemistry - January 3, 2021 Category: Biochemistry Source Type: research

POEMS Syndrome: Clinical and Laboratory Approach Towards Diagnosis
AbstractPolyneuropathy, organomegaly, endocrinopathy, M protein, skin changes (POEMS) syndrome is a rare multisystem paraneoplastic disorder. Here we describe a case of a 50  year old post-menopausal female who presented with chief complaints of difficulty in walking, getting up from squatting position and tingling sensation of bilateral lower limbs since 1 month. Additional unusual features in the patient included hepatosplenomegaly, endocrinal involvement in the for m of hypothyroidism and elevated estradiol levels for her age. There were skin changes in the form of hyperpigmentation. M protein was not noted on serum e...
Source: Indian Journal of Clinical Biochemistry - January 3, 2021 Category: Biochemistry Source Type: research

Dopamine Gene Polymorphism, Biochemical and Oxidative Stress Parameters in Geriatric Population with and Without Depression: A Pilot Study
AbstractDopamine transporter takes released dopamine back into presynaptic terminals and has been implicated in several aging disorders including depression. The present study was designed to demonstrate dopamine gene polymorphism, its circulatory levels, biochemical and oxidative stress parameters in geriatric population with and without depression. Thirty geriatric patients with depression and thirty age and sex matched normal controls were genotyped for Dopamine Active Transporter (DAT TaqA1 andDAT VNTR) gene polymorphisms using the Polymerase Chain Reaction-Restriction Fragment Length Polymorphism method. The frequency...
Source: Indian Journal of Clinical Biochemistry - January 3, 2021 Category: Biochemistry Source Type: research