Indian Journal of Clinical Biochemistry This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

“Silent Hypoxemia” Leads to Vicious Cycle of Infection, Coagulopathy and Cytokine Storm in COVID-19: Can Prophylactic Oxygen Therapy Prevent It?
AbstractHumankind is facing its worst pandemic of the twenty-first century, due to infection of a novel coronavirus named as SARS-CoV2, started from Wuhan in China. Till now, 15 million people are infected, causing more than 600,000 deaths. The disease, commonly known as, COVID-19, was initially thought to be associated with ARDS only, but later on revealed to have many unexplained and atypical clinical features like coagulopathy and cytokinemia, leading to multi-organ involvements. The patients also suffer from ‘Silent Hypoxemia’, where there is no immediate respiratory signs and symptoms even though alarmingly low Sp...
Source: Indian Journal of Clinical Biochemistry - September 21, 2021 Category: Biochemistry Source Type: research

Correction to: “Silent Hypoxemia” Leads to Vicious Cycle of Infection, Coagulopathy and Cytokine Storm in COVID-19: Can Prophylactic Oxygen Therapy Prevent It?
The original article was published incorrectly. (Source: Indian Journal of Clinical Biochemistry)
Source: Indian Journal of Clinical Biochemistry - September 21, 2021 Category: Biochemistry Source Type: research

Relation Between Methylenetetrahydrofolate Reductase Polymorphisms (C677T and A1298C) and Migraine Susceptibility
AbstractMigraine is a neurological disorder which impairs the patient ’s quality of life. Several association studies investigating the association between MTHFR gene C677T and A1298C polymorphisms and susceptibility to migraine were published. But the results were conflicting, so authors performed a meta-analysis of published case control studies to find out the ex act association between MTHFR polymorphism and migraine susceptibility. Four databases were searched for suitable studies up to December, 2018. Odds ratios (OR) with 95% confidence intervals (CI) was calculated adopting additive, homozygote, co-dominant, domi...
Source: Indian Journal of Clinical Biochemistry - September 20, 2021 Category: Biochemistry Source Type: research

Impact of COVID-19 on Clinical Biochemistry: Indian Scenario
(Source: Indian Journal of Clinical Biochemistry)
Source: Indian Journal of Clinical Biochemistry - September 6, 2021 Category: Biochemistry Source Type: research

Assessment of Role and Efficacy of Curcumin and Quercetin in Preventing Lead-Induced Oxidative Stress in Rats
AbstractLead (Pb) is found in almost all phases in environment and biological systems. Pb stimulated oxidative stress is a state that involves the generation of free radicals beyond the permissible limits, which can deplete the antioxidant reserves and can result in oxidative stress, thus hampering the ability of the biological system to reverse the result. Exposure of rats to Pb (25  mg/kg body weight) for 8 weeks caused an increase in Pb levels in blood and brain. Activity of delta-aminolevulinic acid dehydratase (δ-ALAD) and antioxidant enzymes such as Superoxide dismutase (SOD) and Catalase (CAT) decreased in the bl...
Source: Indian Journal of Clinical Biochemistry - September 1, 2021 Category: Biochemistry Source Type: research

Long Noncoding RNAs CAT2064 and CAT2042 may Function as Diagnostic Biomarkers for Prostate Cancer by Affecting Target MicrorRNAs
AbstractProstate cancer (PCa) is the second most common cancer in men throughout the world, and the main cause of cancer death. Long noncoding RNAs (lncRNAs) act as crucial regulators in many human cancers. In this research, we measured the expression level of novel lncRNAs and their associated micro-RNAs (miRNAs) in PCa. In the present research, three lncRNAs were selected using the Mitranscriptome projec (CAT2064, CAT2042, and CAT2164.2). Samples of prostate tissue (20 PCa, and 20 BPH) and blood (14 PCa, and 14 BPH) were collected and the Real-time Quantitative Polymerase Chain Reaction (RT-qPCR) was used to measure the ...
Source: Indian Journal of Clinical Biochemistry - August 24, 2021 Category: Biochemistry Source Type: research

Sequential Profiling of Anti-SARS CoV-2 IgG Antibody in Post COVID-19 Patients
This study determines the expression level and sustainability of anti-receptor binding domain (RBD) SARS CoV-2 IgG in post COVID-19 patients. Anti-RBD SARS CoV-2 IgG antibodies in patient serum were analysed by standardised indirect ELISA using SARS CoV-2 spike receptor binding domain protein and HRP conjugated anti-human IgG antibody (anti-h IgG). The study was conducted using 35 adult patient samples with confirmed SARS CoV-2 infection. Additionally, correlation between antibody response after each stage and disease symptoms in post COVID-19 patients were studied. Maximum antibody titre was seen at Day 40 and decreased r...
Source: Indian Journal of Clinical Biochemistry - August 18, 2021 Category: Biochemistry Source Type: research

Risk Factors for Lead Toxicity and its Effect on Neurobehavior in Indian Children
AbstractLead (Pb) is profoundly used heavy metal despite its known toxic effects. Children in particular are more susceptible to Pb toxicity. Thus, the present study was carried out to estimate the prevalence of lead toxicity in Indian children, to observe serum levels of biochemical parameters and to evaluate psychopathological implications of Pb toxicity using population specific scale —Childhood Psychopathological Measurement Schedule (CPMS) in children. Children between 9 and 15 years of age were included in the study (N = 70). Demographic details and information regarding the source of lead exposure were collec...
Source: Indian Journal of Clinical Biochemistry - August 3, 2021 Category: Biochemistry Source Type: research

A Molecular Insight of the Role of PIN-1 Promoter Polymorphism ( − 667C  & gt;  T; rs2233679) in Chronic Kidney Disease Patients with Secondary Hyperparathyroidism
AbstractMurine studies stipulate Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 (PIN-1) as a key mediator of PTH mRNA stability and is acknowledged by genome-wide-association-studies as secondary hyperparathyroidism defining trait in chronic kidney disease. Therefore, we hypothesize that molecular variants of the PIN-1 gene might affect the incidence and predisposition to secondary hyperparathyroidism in chronic renal insufficiency. A total of 281 adult participants, including 124 chronic kidney disease patients with secondary hyperparathyroidism and 157 healthy controls, were genotyped using polymerase chain react...
Source: Indian Journal of Clinical Biochemistry - August 3, 2021 Category: Biochemistry Source Type: research

Evaluation of Sigma Metrics and Westgard Rule Selection and Implementation of Internal Quality Control in Clinical Chemistry Reference Laboratory, Ethiopian Public Health Institute
AbstractClinical laboratories are mandated to deliver accurate, reliable, timely and correctly reported result which, used in decision making for disease screening, diagnosis and monitoring. With aid of six sigma principles and metrics it is possible to assess the quality laboratory process and the quality control that is needed to ensure that the desired quality is achieved. Thus, this study was undertaken to evaluate the performance of biochemical parameters by calculating the sigma metrics of individual parameters using internal quality control (IQC) and Proficiency Testing (PT) results. The sigma metrics of 21 clinical...
Source: Indian Journal of Clinical Biochemistry - August 2, 2021 Category: Biochemistry Source Type: research

Optimization of Salivary Electrochemical Analysis and its Collection Protocol in Type II Diabetes
AbstractIn the last few decades, saliva due to its non-invasive nature has been highlighted as an important diagnostic biofluid and significant alteration in its composition has been reported with the progression of diabetes. We have investigated the best protocol for saliva collection, handling and processing to leverage its full potential for measuring salivary electrochemical parameters (SEPs) that serve as proxy indicators for fasting blood glucose levels (BGLs) in the body. SEPs such as pH, oxidation –reduction potential (ORP), conductivity and concentrations of Na+, K+ and Ca++ ions were examined in a total of 60 a...
Source: Indian Journal of Clinical Biochemistry - July 30, 2021 Category: Biochemistry Source Type: research

Single-Point Insulin Sensitivity Estimator (SPISE) as a Feasible Marker of Insulin Resistance in Adult Metabolic Syndrome: Evaluated in a Hospital Based Cross-Sectional Pilot Study at Tertiary Care Centre of Uttarakhand
This study was planned to evaluate whether SPISE could be a useful potential low-cost indicator for predicting MetS with IR patients in Indian population. Eighty-three participants from outpatient care  of AIIMS Rishikesh were evaluated after informed consent. They were divided into Metabolic syndrome (n = 56) and Non Metabolic Syndrome(n = 27), using South Asian Modified National Cholesterol Education Program- ATP-III criteria for metabolic syndrome. SPISE index, HOMA-IR, Insulin Resista nce Index, Triglycerides to high-density lipoproteins cholesterol ratio (TG/HDL-C) were calculated for all the subjects. Receiv...
Source: Indian Journal of Clinical Biochemistry - July 15, 2021 Category: Biochemistry Source Type: research

Smouldering Myeloma: Chasing a Hyperviscous Sample
AbstractSerum hyperviscosity is a rare laboratory finding. Amongst several causes of serum hyperviscosity, malignant disorders are quite common. Monoclonal gammopathy is a family of disorders in which monoclonal gammopathy of unknown significance (MGUS) and smoldering myeloma are the asymptomatic variants whereas multiple myeloma is the malignant variant showing different signs and symptoms related to bone lesions, renal failure and anemia. Initially during sample preparation, pipetting of a serum sample was found to be cumbersome. This sample during routine analysis in the automated analyser flagged repeated alarms for cl...
Source: Indian Journal of Clinical Biochemistry - July 14, 2021 Category: Biochemistry Source Type: research

Correction to: Combined Analysis of Anti SARS-CoV-2 IgG and IgM Responses in COVID19 Patients in India
An amendment to this paper has been published and can be accessed via the original article. (Source: Indian Journal of Clinical Biochemistry)
Source: Indian Journal of Clinical Biochemistry - July 7, 2021 Category: Biochemistry Source Type: research

Separated serum was used for the analysisCombined Analysis of Anti SARS-CoV-2 IgG and IgM Responses in COVID19 Patients in India
AbstractCoronavirus disease 2019 (COVID-19) caused by Severe Acute Respiratory Syndrome Coronavirus  2 (SARS-CoV-2) is a global health problem, India being the second most affected country. The kinetics of antibody response to SARS-CoV-2 in Indian population is not studied yet. To understand serological response in relation to age, gender, time period and severity of disease, Roche Elecsys anti-S ARS-CoV-2 test was used which analysed both IgM and IgG. One hundred and three COVID-19 patients were enrolled. Seropositivity was seen in 64% of patients, with 33% at ≤ 7 days, 62% between 8 and 15 days and 81% at ≥�...
Source: Indian Journal of Clinical Biochemistry - June 18, 2021 Category: Biochemistry Source Type: research