Characterisation of mesenchymal stem cells conditioned media obtained at different conditioning times: their effect on glial cells in < em > in vitro < /em > scratch model
In this study, the bone marrow mesenchymal stem cells conditioned media (BMMSC-CM) obtained by conditioning for 24(CM24), 48(CM48) and 72(CM72) hours was characterised. In vitro, the impact of BMMSC-CM on the astrocyte migratory response and oligodendrocyte density was evaluated using the scratch model. The proteomic profiles of individual secretomes were analysed by mass spectrometry and the concentrations of four selected neurotrophins (BDNF, NGF, GDNF and VEGF) were determined by ELISA. Our results revealed an increased number of proteins at CM72, many of which are involved in neuroregenerative processes. ELISA document...
Source: Growth Factors - February 24, 2023 Category: Research Authors: Eva Sz ékiová Zuzana Michalov á Juraj Bla ško Rastislav Mucha Lucia Slovinsk á Martin Kello Ivo Vanick ý Source Type: research
