A novel tracking and analysis system for time-lapse cellular imaging of Schizosaccharomyces pombe
In this study, fundamental image processing techniques were amalgamated to develop software capable of inferring cell cycle alterations in fission yeasts exhibiting equipartition during division. These methods, exclusively relying on bright-field images as input, could track parent-progeny relationships after cellular division through the assessment of temporal morphological transformation of these cells. In the application of this technique, the software was employed for calculate the intracellular fluorescent dots during every stage of the cell cycle, leveraging the yeast strain GFP-fused Swi6, which is present in cells ...
Source: Genes and Genetic Systems - February 21, 2024 Category: Genetics & Stem Cells Authors: Kei Taniguchi Takuya Kajitani Takahito Ayano Toshiyuki Yoshida Masaya Oki Source Type: research
RNA synthesis in liposomes with negatively charged lipids after fusion via freezing-thawing
In this study, we investigated the effect of POPG on the supply of components required for T7 RNA polymerase reactions via F/T by flow cytometry analysis. We found that the addition of POPG to liposome preparations reduced the efficiency of RNA synthesis. In addition, DNA was concentrated during F/T and RNA synthesis occurred after F/T in liposomes composed of POPC and POPG. Our results provide new insights for more efficient supply of substrates and enzymes by the F/T method, thereby increasing the utility of the F/T method for the construction of recursive bioreactors.PMID:38382922 | DOI:10.1266/ggs.23-00297 (Source: Gen...
Source: Genes and Genetic Systems - February 21, 2024 Category: Genetics & Stem Cells Authors: Gakushi Tsuji Ayu Shimomura Shota Fukuoka Masaya Oki Source Type: research
Current research on mechanisms of limb bud development; future difficulties to face over the coming 10 years
Genes Genet Syst. 2024 Feb 21. doi: 10.1266/ggs.23-00287. Online ahead of print.ABSTRACTThe developmental mechanisms of limb buds have been studied in developmental biology as a great model of pattern formation. Chick embryos have contributed to the discovery of new principles in developmental biology, as it is easy to observe live embryos and manipulate embryonic tissues. Herein, we outline the current findings and future issues over the next 10 years regarding three themes, based on our research: 1) limb positioning, 2) proximal-distal limb elongation, and 3) digit identity determination. First, how hindlimb position is ...
Source: Genes and Genetic Systems - February 21, 2024 Category: Genetics & Stem Cells Authors: Takayuki Suzuki Source Type: research
IMD2, which is located near the boundary of heterochromatin regions, is regulated by the use of multiple HAT-related factors
In this study, we evaluated the effects of the SAGA complex, a histone acetyltransferase (HAT) complex, and its relationship with other histone-modifying enzymes to clarify the mechanism underlying boundary regulation of the IMD2 gene on the right subtelomere of chromosome VIII. We found that Spt8, a component of the SAGA complex, is important for boundary formation in this region and that the inclusion of Spt8 in the SAGA complex is more important than its interaction with TATA-binding protein and TFIIS. In addition to SAGA, various HAT-related factors, such as NuA4 and Rtt109, also functioned in this region. In particula...
Source: Genes and Genetic Systems - February 21, 2024 Category: Genetics & Stem Cells Authors: Takahito Ayano Masaya Oki Source Type: research
Pigmentation of soybean seed coats via mutation that abolishes production of multiple-phased siRNAs of the chalcone synthase gene
Genes Genet Syst. 2024 Feb 21. doi: 10.1266/ggs.23-00260. Online ahead of print.ABSTRACTLack of pigmentation in seed coats of soybean is caused by natural RNA silencing of the chalcone synthase (CHS) genes. This phenomenon is an evolutionary consequence of structural changes in DNA that resulted in the production of double-stranded RNAs (dsRNAs) that trigger RNA degradation. Here we determined that a mutant with pigmented seed coats derived from a cultivar that lacked the pigmentation had a deletion between DNA regions ICHS1 and a cytochrome P450 gene; the deletion included GmIRCHS, a candidate gene that triggers CHS RNA s...
Source: Genes and Genetic Systems - February 21, 2024 Category: Genetics & Stem Cells Authors: Mashiro Yuhazu Shun Mikuriya Ayumi Mori Maria Stefanie Dwiyanti Mineo Senda Akira Kanazawa Source Type: research
A novel tracking and analysis system for time-lapse cellular imaging of Schizosaccharomyces pombe
In this study, fundamental image processing techniques were amalgamated to develop software capable of inferring cell cycle alterations in fission yeasts exhibiting equipartition during division. These methods, exclusively relying on bright-field images as input, could track parent-progeny relationships after cellular division through the assessment of temporal morphological transformation of these cells. In the application of this technique, the software was employed for calculate the intracellular fluorescent dots during every stage of the cell cycle, leveraging the yeast strain GFP-fused Swi6, which is present in cells ...
Source: Genes and Genetic Systems - February 21, 2024 Category: Genetics & Stem Cells Authors: Kei Taniguchi Takuya Kajitani Takahito Ayano Toshiyuki Yoshida Masaya Oki Source Type: research
RNA synthesis in liposomes with negatively charged lipids after fusion via freezing-thawing
In this study, we investigated the effect of POPG on the supply of components required for T7 RNA polymerase reactions via F/T by flow cytometry analysis. We found that the addition of POPG to liposome preparations reduced the efficiency of RNA synthesis. In addition, DNA was concentrated during F/T and RNA synthesis occurred after F/T in liposomes composed of POPC and POPG. Our results provide new insights for more efficient supply of substrates and enzymes by the F/T method, thereby increasing the utility of the F/T method for the construction of recursive bioreactors.PMID:38382922 | DOI:10.1266/ggs.23-00297 (Source: Gen...
Source: Genes and Genetic Systems - February 21, 2024 Category: Genetics & Stem Cells Authors: Gakushi Tsuji Ayu Shimomura Shota Fukuoka Masaya Oki Source Type: research
Current research on mechanisms of limb bud development; future difficulties to face over the coming 10 years
Genes Genet Syst. 2024 Feb 21. doi: 10.1266/ggs.23-00287. Online ahead of print.ABSTRACTThe developmental mechanisms of limb buds have been studied in developmental biology as a great model of pattern formation. Chick embryos have contributed to the discovery of new principles in developmental biology, as it is easy to observe live embryos and manipulate embryonic tissues. Herein, we outline the current findings and future issues over the next 10 years regarding three themes, based on our research: 1) limb positioning, 2) proximal-distal limb elongation, and 3) digit identity determination. First, how hindlimb position is ...
Source: Genes and Genetic Systems - February 21, 2024 Category: Genetics & Stem Cells Authors: Takayuki Suzuki Source Type: research
IMD2, which is located near the boundary of heterochromatin regions, is regulated by the use of multiple HAT-related factors
In this study, we evaluated the effects of the SAGA complex, a histone acetyltransferase (HAT) complex, and its relationship with other histone-modifying enzymes to clarify the mechanism underlying boundary regulation of the IMD2 gene on the right subtelomere of chromosome VIII. We found that Spt8, a component of the SAGA complex, is important for boundary formation in this region and that the inclusion of Spt8 in the SAGA complex is more important than its interaction with TATA-binding protein and TFIIS. In addition to SAGA, various HAT-related factors, such as NuA4 and Rtt109, also functioned in this region. In particula...
Source: Genes and Genetic Systems - February 21, 2024 Category: Genetics & Stem Cells Authors: Takahito Ayano Masaya Oki Source Type: research
Pigmentation of soybean seed coats via mutation that abolishes production of multiple-phased siRNAs of the chalcone synthase gene
Genes Genet Syst. 2024 Feb 21. doi: 10.1266/ggs.23-00260. Online ahead of print.ABSTRACTLack of pigmentation in seed coats of soybean is caused by natural RNA silencing of the chalcone synthase (CHS) genes. This phenomenon is an evolutionary consequence of structural changes in DNA that resulted in the production of double-stranded RNAs (dsRNAs) that trigger RNA degradation. Here we determined that a mutant with pigmented seed coats derived from a cultivar that lacked the pigmentation had a deletion between DNA regions ICHS1 and a cytochrome P450 gene; the deletion included GmIRCHS, a candidate gene that triggers CHS RNA s...
Source: Genes and Genetic Systems - February 21, 2024 Category: Genetics & Stem Cells Authors: Mashiro Yuhazu Shun Mikuriya Ayumi Mori Maria Stefanie Dwiyanti Mineo Senda Akira Kanazawa Source Type: research
A novel tracking and analysis system for time-lapse cellular imaging of Schizosaccharomyces pombe
In this study, fundamental image processing techniques were amalgamated to develop software capable of inferring cell cycle alterations in fission yeasts exhibiting equipartition during division. These methods, exclusively relying on bright-field images as input, could track parent-progeny relationships after cellular division through the assessment of temporal morphological transformation of these cells. In the application of this technique, the software was employed for calculate the intracellular fluorescent dots during every stage of the cell cycle, leveraging the yeast strain GFP-fused Swi6, which is present in cells ...
Source: Genes and Genetic Systems - February 21, 2024 Category: Genetics & Stem Cells Authors: Kei Taniguchi Takuya Kajitani Takahito Ayano Toshiyuki Yoshida Masaya Oki Source Type: research
Multifaceted Roles of Transposons in Mammalian Evolution and the Future of Transposon Research
Genes Genet Syst. 2023;98(6):287. doi: 10.1266/ggs.98.287.NO ABSTRACTPMID:38346754 | DOI:10.1266/ggs.98.287 (Source: Genes and Genetic Systems)
Source: Genes and Genetic Systems - February 12, 2024 Category: Genetics & Stem Cells Authors: Kei Fukuda Source Type: research
Author Index (vol. 98, 2023)
Genes Genet Syst. 2023;98(6):367-368. doi: 10.1266/ggs.98.367.NO ABSTRACTPMID:38346756 | DOI:10.1266/ggs.98.367 (Source: Genes and Genetic Systems)
Source: Genes and Genetic Systems - February 12, 2024 Category: Genetics & Stem Cells Source Type: research
Multifaceted Roles of Transposons in Mammalian Evolution and the Future of Transposon Research
Genes Genet Syst. 2023;98(6):287. doi: 10.1266/ggs.98.287.NO ABSTRACTPMID:38346754 | DOI:10.1266/ggs.98.287 (Source: Genes and Genetic Systems)
Source: Genes and Genetic Systems - February 12, 2024 Category: Genetics & Stem Cells Authors: Kei Fukuda Source Type: research
Author Index (vol. 98, 2023)
Genes Genet Syst. 2023;98(6):367-368. doi: 10.1266/ggs.98.367.NO ABSTRACTPMID:38346756 | DOI:10.1266/ggs.98.367 (Source: Genes and Genetic Systems)
Source: Genes and Genetic Systems - February 12, 2024 Category: Genetics & Stem Cells Source Type: research
