Budding Yeast Cap Analysis of Gene Expression Dataset of Two Cell Types
Genes Genet Syst. 2024 Mar 7. doi: 10.1266/ggs.24-00020. Online ahead of print.ABSTRACTThe budding yeast Saccharomyces cerevisiae is an excellent model organism for studying chromatin regulation with high-resolution genome-wide analyses. Since newly generated genome-wide data are often compared with publicly available datasets, expanding our dataset repertoire will be beneficial for the field. Information on transcription start sites (TSSs) determined at base pair resolution is essential for elucidating mechanisms of transcription and related chromatin regulation, yet no datasets that cover two different cell types are ava...
Source: Genes and Genetic Systems - March 6, 2024 Category: Genetics & Stem Cells Authors: Kei Kawakami Shin-Ichi Maeda Yoshiko Tanimoto Mitsuhiro Shimizu Hiroaki Kato Source Type: research
Budding Yeast Cap Analysis of Gene Expression Dataset of Two Cell Types
Genes Genet Syst. 2024 Mar 7. doi: 10.1266/ggs.24-00020. Online ahead of print.ABSTRACTThe budding yeast Saccharomyces cerevisiae is an excellent model organism for studying chromatin regulation with high-resolution genome-wide analyses. Since newly generated genome-wide data are often compared with publicly available datasets, expanding our dataset repertoire will be beneficial for the field. Information on transcription start sites (TSSs) determined at base pair resolution is essential for elucidating mechanisms of transcription and related chromatin regulation, yet no datasets that cover two different cell types are ava...
Source: Genes and Genetic Systems - March 6, 2024 Category: Genetics & Stem Cells Authors: Kei Kawakami Shin-Ichi Maeda Yoshiko Tanimoto Mitsuhiro Shimizu Hiroaki Kato Source Type: research
Budding Yeast Cap Analysis of Gene Expression Dataset of Two Cell Types
Genes Genet Syst. 2024 Mar 7. doi: 10.1266/ggs.24-00020. Online ahead of print.ABSTRACTThe budding yeast Saccharomyces cerevisiae is an excellent model organism for studying chromatin regulation with high-resolution genome-wide analyses. Since newly generated genome-wide data are often compared with publicly available datasets, expanding our dataset repertoire will be beneficial for the field. Information on transcription start sites (TSSs) determined at base pair resolution is essential for elucidating mechanisms of transcription and related chromatin regulation, yet no datasets that cover two different cell types are ava...
Source: Genes and Genetic Systems - March 6, 2024 Category: Genetics & Stem Cells Authors: Kei Kawakami Shin-Ichi Maeda Yoshiko Tanimoto Mitsuhiro Shimizu Hiroaki Kato Source Type: research
Budding Yeast Cap Analysis of Gene Expression Dataset of Two Cell Types
Genes Genet Syst. 2024 Mar 7. doi: 10.1266/ggs.24-00020. Online ahead of print.ABSTRACTThe budding yeast Saccharomyces cerevisiae is an excellent model organism for studying chromatin regulation with high-resolution genome-wide analyses. Since newly generated genome-wide data are often compared with publicly available datasets, expanding our dataset repertoire will be beneficial for the field. Information on transcription start sites (TSSs) determined at base pair resolution is essential for elucidating mechanisms of transcription and related chromatin regulation, yet no datasets that cover two different cell types are ava...
Source: Genes and Genetic Systems - March 6, 2024 Category: Genetics & Stem Cells Authors: Kei Kawakami Shin-Ichi Maeda Yoshiko Tanimoto Mitsuhiro Shimizu Hiroaki Kato Source Type: research
Budding Yeast Cap Analysis of Gene Expression Dataset of Two Cell Types
Genes Genet Syst. 2024 Mar 7. doi: 10.1266/ggs.24-00020. Online ahead of print.ABSTRACTThe budding yeast Saccharomyces cerevisiae is an excellent model organism for studying chromatin regulation with high-resolution genome-wide analyses. Since newly generated genome-wide data are often compared with publicly available datasets, expanding our dataset repertoire will be beneficial for the field. Information on transcription start sites (TSSs) determined at base pair resolution is essential for elucidating mechanisms of transcription and related chromatin regulation, yet no datasets that cover two different cell types are ava...
Source: Genes and Genetic Systems - March 6, 2024 Category: Genetics & Stem Cells Authors: Kei Kawakami Shin-Ichi Maeda Yoshiko Tanimoto Mitsuhiro Shimizu Hiroaki Kato Source Type: research
Budding Yeast Cap Analysis of Gene Expression Dataset of Two Cell Types
Genes Genet Syst. 2024 Mar 7. doi: 10.1266/ggs.24-00020. Online ahead of print.ABSTRACTThe budding yeast Saccharomyces cerevisiae is an excellent model organism for studying chromatin regulation with high-resolution genome-wide analyses. Since newly generated genome-wide data are often compared with publicly available datasets, expanding our dataset repertoire will be beneficial for the field. Information on transcription start sites (TSSs) determined at base pair resolution is essential for elucidating mechanisms of transcription and related chromatin regulation, yet no datasets that cover two different cell types are ava...
Source: Genes and Genetic Systems - March 6, 2024 Category: Genetics & Stem Cells Authors: Kei Kawakami Shin-Ichi Maeda Yoshiko Tanimoto Mitsuhiro Shimizu Hiroaki Kato Source Type: research
METTL21C mediates the occurrence of autophagy and formation of slow-twitch muscle fibers after exercise
This study investigated the role of methyltransferase-like 21C (METTL21C) in skeletal muscle of mice after exercise and the potential mechanism. First, muscle samples were collected at 2, 4, and 6 weeks after exercise, liver glycogen, muscle glycogen, blood lactic acid (BLA) and triglyceride (TG) were assessed. Moreover, the expression levels of autophagy markers and METTL21C in skeletal muscle were analyzed. The results showed that the expressions of METTL21C and MYH7 in the gastrocnemius muscle of mice in the exercise group were significantly higher than that in the control group after exercise, which suggested that long...
Source: Genes and Genetic Systems - February 28, 2024 Category: Genetics & Stem Cells Authors: Jing Qu Shuai Dang Yuan-Yuan Sun Tao Zhang Hai Jiang Hong-Zhao Lu Source Type: research
METTL21C mediates the occurrence of autophagy and formation of slow-twitch muscle fibers after exercise
This study investigated the role of methyltransferase-like 21C (METTL21C) in skeletal muscle of mice after exercise and the potential mechanism. First, muscle samples were collected at 2, 4, and 6 weeks after exercise, liver glycogen, muscle glycogen, blood lactic acid (BLA) and triglyceride (TG) were assessed. Moreover, the expression levels of autophagy markers and METTL21C in skeletal muscle were analyzed. The results showed that the expressions of METTL21C and MYH7 in the gastrocnemius muscle of mice in the exercise group were significantly higher than that in the control group after exercise, which suggested that long...
Source: Genes and Genetic Systems - February 28, 2024 Category: Genetics & Stem Cells Authors: Jing Qu Shuai Dang Yuan-Yuan Sun Tao Zhang Hai Jiang Hong-Zhao Lu Source Type: research
METTL21C mediates the occurrence of autophagy and formation of slow-twitch muscle fibers after exercise
This study investigated the role of methyltransferase-like 21C (METTL21C) in skeletal muscle of mice after exercise and the potential mechanism. First, muscle samples were collected at 2, 4, and 6 weeks after exercise, liver glycogen, muscle glycogen, blood lactic acid (BLA) and triglyceride (TG) were assessed. Moreover, the expression levels of autophagy markers and METTL21C in skeletal muscle were analyzed. The results showed that the expressions of METTL21C and MYH7 in the gastrocnemius muscle of mice in the exercise group were significantly higher than that in the control group after exercise, which suggested that long...
Source: Genes and Genetic Systems - February 28, 2024 Category: Genetics & Stem Cells Authors: Jing Qu Shuai Dang Yuan-Yuan Sun Tao Zhang Hai Jiang Hong-Zhao Lu Source Type: research
METTL21C mediates the occurrence of autophagy and formation of slow-twitch muscle fibers after exercise
This study investigated the role of methyltransferase-like 21C (METTL21C) in skeletal muscle of mice after exercise and the potential mechanism. First, muscle samples were collected at 2, 4, and 6 weeks after exercise, liver glycogen, muscle glycogen, blood lactic acid (BLA) and triglyceride (TG) were assessed. Moreover, the expression levels of autophagy markers and METTL21C in skeletal muscle were analyzed. The results showed that the expressions of METTL21C and MYH7 in the gastrocnemius muscle of mice in the exercise group were significantly higher than that in the control group after exercise, which suggested that long...
Source: Genes and Genetic Systems - February 28, 2024 Category: Genetics & Stem Cells Authors: Jing Qu Shuai Dang Yuan-Yuan Sun Tao Zhang Hai Jiang Hong-Zhao Lu Source Type: research
METTL21C mediates the occurrence of autophagy and formation of slow-twitch muscle fibers after exercise
This study investigated the role of methyltransferase-like 21C (METTL21C) in skeletal muscle of mice after exercise and the potential mechanism. First, muscle samples were collected at 2, 4, and 6 weeks after exercise, liver glycogen, muscle glycogen, blood lactic acid (BLA) and triglyceride (TG) were assessed. Moreover, the expression levels of autophagy markers and METTL21C in skeletal muscle were analyzed. The results showed that the expressions of METTL21C and MYH7 in the gastrocnemius muscle of mice in the exercise group were significantly higher than that in the control group after exercise, which suggested that long...
Source: Genes and Genetic Systems - February 28, 2024 Category: Genetics & Stem Cells Authors: Jing Qu Shuai Dang Yuan-Yuan Sun Tao Zhang Hai Jiang Hong-Zhao Lu Source Type: research
RNA synthesis in liposomes with negatively charged lipids after fusion via freezing-thawing
In this study, we investigated the effect of POPG on the supply of components required for T7 RNA polymerase reactions via F/T by flow cytometry analysis. We found that the addition of POPG to liposome preparations reduced the efficiency of RNA synthesis. In addition, DNA was concentrated during F/T and RNA synthesis occurred after F/T in liposomes composed of POPC and POPG. Our results provide new insights for more efficient supply of substrates and enzymes by the F/T method, thereby increasing the utility of the F/T method for the construction of recursive bioreactors.PMID:38382922 | DOI:10.1266/ggs.23-00297 (Source: Gen...
Source: Genes and Genetic Systems - February 21, 2024 Category: Genetics & Stem Cells Authors: Gakushi Tsuji Ayu Shimomura Shota Fukuoka Masaya Oki Source Type: research
Current research on mechanisms of limb bud development; future difficulties to face over the coming 10 years
Genes Genet Syst. 2024 Feb 21. doi: 10.1266/ggs.23-00287. Online ahead of print.ABSTRACTThe developmental mechanisms of limb buds have been studied in developmental biology as a great model of pattern formation. Chick embryos have contributed to the discovery of new principles in developmental biology, as it is easy to observe live embryos and manipulate embryonic tissues. Herein, we outline the current findings and future issues over the next 10 years regarding three themes, based on our research: 1) limb positioning, 2) proximal-distal limb elongation, and 3) digit identity determination. First, how hindlimb position is ...
Source: Genes and Genetic Systems - February 21, 2024 Category: Genetics & Stem Cells Authors: Takayuki Suzuki Source Type: research
IMD2, which is located near the boundary of heterochromatin regions, is regulated by the use of multiple HAT-related factors
In this study, we evaluated the effects of the SAGA complex, a histone acetyltransferase (HAT) complex, and its relationship with other histone-modifying enzymes to clarify the mechanism underlying boundary regulation of the IMD2 gene on the right subtelomere of chromosome VIII. We found that Spt8, a component of the SAGA complex, is important for boundary formation in this region and that the inclusion of Spt8 in the SAGA complex is more important than its interaction with TATA-binding protein and TFIIS. In addition to SAGA, various HAT-related factors, such as NuA4 and Rtt109, also functioned in this region. In particula...
Source: Genes and Genetic Systems - February 21, 2024 Category: Genetics & Stem Cells Authors: Takahito Ayano Masaya Oki Source Type: research
Pigmentation of soybean seed coats via mutation that abolishes production of multiple-phased siRNAs of the chalcone synthase gene
Genes Genet Syst. 2024 Feb 21. doi: 10.1266/ggs.23-00260. Online ahead of print.ABSTRACTLack of pigmentation in seed coats of soybean is caused by natural RNA silencing of the chalcone synthase (CHS) genes. This phenomenon is an evolutionary consequence of structural changes in DNA that resulted in the production of double-stranded RNAs (dsRNAs) that trigger RNA degradation. Here we determined that a mutant with pigmented seed coats derived from a cultivar that lacked the pigmentation had a deletion between DNA regions ICHS1 and a cytochrome P450 gene; the deletion included GmIRCHS, a candidate gene that triggers CHS RNA s...
Source: Genes and Genetic Systems - February 21, 2024 Category: Genetics & Stem Cells Authors: Mashiro Yuhazu Shun Mikuriya Ayumi Mori Maria Stefanie Dwiyanti Mineo Senda Akira Kanazawa Source Type: research
