Development of an isogenic human cell trio that models polyglutamine disease
This study thus demonstrates the potential of UKiS, which is a beneficial platform for the efficient development of cell models not only for polyQ diseases but also for any other genetic diseases, which may accelerate our deeper understanding of disease mechanisms and cell-based screening for therapeutic drugs.PMID:37821389 | DOI:10.1266/ggs.22-00030 (Source: Genes and Genetic Systems)
Source: Genes and Genetic Systems - October 11, 2023 Category: Genetics & Stem Cells Authors: Tomoyuki Ohno Takeshi Nakane Taichi Akase Hikaru Kurasawa Yasunori Aizawa Source Type: research
Development of an isogenic human cell trio that models polyglutamine disease
This study thus demonstrates the potential of UKiS, which is a beneficial platform for the efficient development of cell models not only for polyQ diseases but also for any other genetic diseases, which may accelerate our deeper understanding of disease mechanisms and cell-based screening for therapeutic drugs.PMID:37821389 | DOI:10.1266/ggs.22-00030 (Source: Genes and Genetic Systems)
Source: Genes and Genetic Systems - October 11, 2023 Category: Genetics & Stem Cells Authors: Tomoyuki Ohno Takeshi Nakane Taichi Akase Hikaru Kurasawa Yasunori Aizawa Source Type: research
Development of an isogenic human cell trio that models polyglutamine disease
This study thus demonstrates the potential of UKiS, which is a beneficial platform for the efficient development of cell models not only for polyQ diseases but also for any other genetic diseases, which may accelerate our deeper understanding of disease mechanisms and cell-based screening for therapeutic drugs.PMID:37821389 | DOI:10.1266/ggs.22-00030 (Source: Genes and Genetic Systems)
Source: Genes and Genetic Systems - October 11, 2023 Category: Genetics & Stem Cells Authors: Tomoyuki Ohno Takeshi Nakane Taichi Akase Hikaru Kurasawa Yasunori Aizawa Source Type: research
Fine mapping of Green a, Ga, on chromosome 27 in Bombyx mori
Genes Genet Syst. 2023 Oct 6. doi: 10.1266/ggs.23-00060. Online ahead of print.ABSTRACTSome strains of silkworms produce green cocoons of varying intensities. This results from quantitative and qualitative differences in flavonoid pigments, which are influenced by the environment and genetic background. We discovered that the appearance of a faint green cocoon is regulated by a gene (G27) located on chromosome 27. Through mating experiments, we found that G27 is identical to an essential flavonoid cocoon gene, Ga. This locus has not been previously described. Furthermore, we narrowed down the Ga region to 438 kbp using mol...
Source: Genes and Genetic Systems - October 9, 2023 Category: Genetics & Stem Cells Authors: Keisuke Mase Chikara Hirayama Junko Narukawa Seigo Kuwazaki Kimiko Yamamoto Source Type: research
Comprehensive analysis of m6A modifications in oral squamous cell carcinoma by MeRIP sequencing
In this study, we examined the relationship between m6A and OSCC. Four pairs of OSCC and adjacent normal tissues were compared by Methylated RNA immunoprecipitation sequencing (MeRIP-seq). Gene Ontology, Kyoto Encyclopedia of Genes and Genomes (KEGG), and Ingenuity Pathway Analysis (IPA) analyses were used to further analyze the MeRIP-seq data. A total of 2,348 different m6A peaks were identified in the OSCC group, including 85 m6A upregulated peaks and 2,263 m6A downregulated peaks. Differentially methylated m6A binding sites were enriched in the coding sequence in proximity to the stop codon of both groups. KEGG analysis...
Source: Genes and Genetic Systems - October 9, 2023 Category: Genetics & Stem Cells Authors: Yang Liu Huiqing Long Xiaogang Zhong Li Yan Lu Yang Yingying Zhang Fangzhi Lou Shihong Luo Xin Jin Source Type: research
Fine mapping of Green a, Ga, on chromosome 27 in Bombyx mori
Genes Genet Syst. 2023 Oct 6. doi: 10.1266/ggs.23-00060. Online ahead of print.ABSTRACTSome strains of silkworms produce green cocoons of varying intensities. This results from quantitative and qualitative differences in flavonoid pigments, which are influenced by the environment and genetic background. We discovered that the appearance of a faint green cocoon is regulated by a gene (G27) located on chromosome 27. Through mating experiments, we found that G27 is identical to an essential flavonoid cocoon gene, Ga. This locus has not been previously described. Furthermore, we narrowed down the Ga region to 438 kbp using mol...
Source: Genes and Genetic Systems - October 9, 2023 Category: Genetics & Stem Cells Authors: Keisuke Mase Chikara Hirayama Junko Narukawa Seigo Kuwazaki Kimiko Yamamoto Source Type: research
Comprehensive analysis of m6A modifications in oral squamous cell carcinoma by MeRIP sequencing
In this study, we examined the relationship between m6A and OSCC. Four pairs of OSCC and adjacent normal tissues were compared by Methylated RNA immunoprecipitation sequencing (MeRIP-seq). Gene Ontology, Kyoto Encyclopedia of Genes and Genomes (KEGG), and Ingenuity Pathway Analysis (IPA) analyses were used to further analyze the MeRIP-seq data. A total of 2,348 different m6A peaks were identified in the OSCC group, including 85 m6A upregulated peaks and 2,263 m6A downregulated peaks. Differentially methylated m6A binding sites were enriched in the coding sequence in proximity to the stop codon of both groups. KEGG analysis...
Source: Genes and Genetic Systems - October 9, 2023 Category: Genetics & Stem Cells Authors: Yang Liu Huiqing Long Xiaogang Zhong Li Yan Lu Yang Yingying Zhang Fangzhi Lou Shihong Luo Xin Jin Source Type: research
Translation of paired box 6 (PAX6) mRNA is IRES-mediated and inhibited by cymarin in breast cancer cells
Genes Genet Syst. 2023 Oct 4. doi: 10.1266/ggs.23-00039. Online ahead of print.ABSTRACTPaired box 6 (PAX6) is a member of the PAX family and plays an essential role in cancer cell cycle progression, colony formation, proliferation and invasion. Its expression is upregulated in many cancers including breast cancer, but the process of PAX6 mRNA translation has rarely been studied. We found that PAX6 translation level increased in MCF-7 breast cancer cells treated with the chemotherapeutic drug adriamycin (ADM), which might be attributable to internal ribosome entry site (IRES)-mediated translation. By modifying a bicistronic...
Source: Genes and Genetic Systems - October 4, 2023 Category: Genetics & Stem Cells Authors: Qi Li Xiao Liu Yun Xu Zihong Lu Sunmin Jiang Ying Yao Source Type: research
Translation of paired box 6 (PAX6) mRNA is IRES-mediated and inhibited by cymarin in breast cancer cells
Genes Genet Syst. 2023 Oct 4. doi: 10.1266/ggs.23-00039. Online ahead of print.ABSTRACTPaired box 6 (PAX6) is a member of the PAX family and plays an essential role in cancer cell cycle progression, colony formation, proliferation and invasion. Its expression is upregulated in many cancers including breast cancer, but the process of PAX6 mRNA translation has rarely been studied. We found that PAX6 translation level increased in MCF-7 breast cancer cells treated with the chemotherapeutic drug adriamycin (ADM), which might be attributable to internal ribosome entry site (IRES)-mediated translation. By modifying a bicistronic...
Source: Genes and Genetic Systems - October 4, 2023 Category: Genetics & Stem Cells Authors: Qi Li Xiao Liu Yun Xu Zihong Lu Sunmin Jiang Ying Yao Source Type: research
Translation of paired box 6 (PAX6) mRNA is IRES-mediated and inhibited by cymarin in breast cancer cells
Genes Genet Syst. 2023 Oct 4. doi: 10.1266/ggs.23-00039. Online ahead of print.ABSTRACTPaired box 6 (PAX6) is a member of the PAX family and plays an essential role in cancer cell cycle progression, colony formation, proliferation and invasion. Its expression is upregulated in many cancers including breast cancer, but the process of PAX6 mRNA translation has rarely been studied. We found that PAX6 translation level increased in MCF-7 breast cancer cells treated with the chemotherapeutic drug adriamycin (ADM), which might be attributable to internal ribosome entry site (IRES)-mediated translation. By modifying a bicistronic...
Source: Genes and Genetic Systems - October 4, 2023 Category: Genetics & Stem Cells Authors: Qi Li Xiao Liu Yun Xu Zihong Lu Sunmin Jiang Ying Yao Source Type: research
Translation of paired box 6 (PAX6) mRNA is IRES-mediated and inhibited by cymarin in breast cancer cells
Genes Genet Syst. 2023 Oct 4. doi: 10.1266/ggs.23-00039. Online ahead of print.ABSTRACTPaired box 6 (PAX6) is a member of the PAX family and plays an essential role in cancer cell cycle progression, colony formation, proliferation and invasion. Its expression is upregulated in many cancers including breast cancer, but the process of PAX6 mRNA translation has rarely been studied. We found that PAX6 translation level increased in MCF-7 breast cancer cells treated with the chemotherapeutic drug adriamycin (ADM), which might be attributable to internal ribosome entry site (IRES)-mediated translation. By modifying a bicistronic...
Source: Genes and Genetic Systems - October 4, 2023 Category: Genetics & Stem Cells Authors: Qi Li Xiao Liu Yun Xu Zihong Lu Sunmin Jiang Ying Yao Source Type: research
Testing immediate dosage compensation in Drosophila miranda via irradiation with heavy-ion beams
In this study, by irradiating Drosophila miranda male flies, which carry young sex chromosomes (the so-called neo-sex chromosomes), with heavy-ion beams, we induced deletions in the neo-Y chromosome to mimic the condition of Y-chromosome degeneration, in which functional neo-Y-linked genes are nonfunctionalized; furthermore, we tested whether their neo-X-linked gametologs were immediately upregulated. Because the males that received 2-Gy iron-ion beam irradiation exhibited lower fertility, we sequenced the genomes and transcriptomes of six F1 males derived from these males. Our pipeline identified 82 neo-Y-linked genes in ...
Source: Genes and Genetic Systems - October 1, 2023 Category: Genetics & Stem Cells Authors: Masafumi Ogawa Kazuhide Tsuneizumi Tomoko Abe Masafumi Nozawa Source Type: research
Expression profiles in knock-down transgenic plants of high and low diversified duplicate genes in Arabidopsis thaliana
Genes Genet Syst. 2023 Sep 29. doi: 10.1266/ggs.23-00019. Online ahead of print.ABSTRACTDuplicated genes show various degrees of functional diversification in plants. We previously identified 1,052 pairs of high diversified duplicates (HDDs) and 600 pairs of low diversified duplicates (LDDs) in Arabidopsis thaliana. Single knock-down of HDDs induced abnormal phenotypic changes because the other gene copy could not compensate for the knock-down effect, while single knock-down of LDDs did not induce abnormal phenotypic changes because of functional compensation by the copy gene. Here, focusing on one pair each of HDDs and LD...
Source: Genes and Genetic Systems - October 1, 2023 Category: Genetics & Stem Cells Authors: Tomoyuki Takeda Akihiro Ezoe Kousuke Hanada Source Type: research
Mechanisms and impacts of genomic changes that are mediated by repetitive sequences in eukaryotes
Genes Genet Syst. 2023;98(3):101. doi: 10.1266/ggs.98.101.NO ABSTRACTPMID:37779087 | DOI:10.1266/ggs.98.101 (Source: Genes and Genetic Systems)
Source: Genes and Genetic Systems - October 1, 2023 Category: Genetics & Stem Cells Authors: Mariko Sasaki Source Type: research
Testing immediate dosage compensation in Drosophila miranda via irradiation with heavy-ion beams
In this study, by irradiating Drosophila miranda male flies, which carry young sex chromosomes (the so-called neo-sex chromosomes), with heavy-ion beams, we induced deletions in the neo-Y chromosome to mimic the condition of Y-chromosome degeneration, in which functional neo-Y-linked genes are nonfunctionalized; furthermore, we tested whether their neo-X-linked gametologs were immediately upregulated. Because the males that received 2-Gy iron-ion beam irradiation exhibited lower fertility, we sequenced the genomes and transcriptomes of six F1 males derived from these males. Our pipeline identified 82 neo-Y-linked genes in ...
Source: Genes and Genetic Systems - October 1, 2023 Category: Genetics & Stem Cells Authors: Masafumi Ogawa Kazuhide Tsuneizumi Tomoko Abe Masafumi Nozawa Source Type: research
