RNA synthesis in liposomes with negatively charged lipids after fusion via freezing-thawing

In this study, we investigated the effect of POPG on the supply of components required for T7 RNA polymerase reactions via F/T by flow cytometry analysis. We found that the addition of POPG to liposome preparations reduced the efficiency of RNA synthesis. In addition, DNA was concentrated during F/T and RNA synthesis occurred after F/T in liposomes composed of POPC and POPG. Our results provide new insights for more efficient supply of substrates and enzymes by the F/T method, thereby increasing the utility of the F/T method for the construction of recursive bioreactors.PMID:38382922 | DOI:10.1266/ggs.23-00297

Source: Genes and Genetic Systems - February 21, 2024 Category: Genetics & Stem Cells Authors: Gakushi Tsuji Ayu Shimomura Shota Fukuoka Masaya Oki Source Type: research