IMD2, which is located near the boundary of heterochromatin regions, is regulated by the use of multiple HAT-related factors

In this study, we evaluated the effects of the SAGA complex, a histone acetyltransferase (HAT) complex, and its relationship with other histone-modifying enzymes to clarify the mechanism underlying boundary regulation of the IMD2 gene on the right subtelomere of chromosome VIII. We found that Spt8, a component of the SAGA complex, is important for boundary formation in this region and that the inclusion of Spt8 in the SAGA complex is more important than its interaction with TATA-binding protein and TFIIS. In addition to SAGA, various HAT-related factors, such as NuA4 and Rtt109, also functioned in this region. In particular, the SAGA complex induced weak IMD2 expression throughout the cell, whereas NuA4 induced strong expression. These results indicate that multiple HATs contribute to the regulation of boundary formation and IMD2 expression on the right subtelomeres of chromosome VIII and that IMD2 expression is determined by the balance between these factors.PMID:38382924 | DOI:10.1266/ggs.23-00284

Source: Genes and Genetic Systems - February 21, 2024 Category: Genetics & Stem Cells Authors: Takahito Ayano Masaya Oki Source Type: research