Erratum: Establishment and application of a rapid assay for GII.4/GII.17 NoV detection based on the combination of CRISPR/Cas13a and isothermal amplification
(Source: Frontiers in Microbiology)
Source: Frontiers in Microbiology - April 2, 2024 Category: Microbiology Source Type: research
Synbiotic regulates gut microbiota in patients with lupus nephritis: an analysis using metagenomic and metabolome sequencing
ConclusionSynbiotics regulate the metabolic functions of intestinal microorganisms in patients with LN and play a role in various biological functions. Synbiotic supplements may be safe and promising candidates for patients with LN. (Source: Frontiers in Microbiology)
Source: Frontiers in Microbiology - April 2, 2024 Category: Microbiology Source Type: research
Association between gut microbiota and gastric cancers: a two-sample Mendelian randomization study
ConclusionOur studies have shown that there is a causal relationship between intestinal flora and gastric cancer at the genetic level. Clostridium sensustricto1, Actinomycetales, Rikenellaceae, Selenomonadales, Negativicutes, and Actinomycetaceae as having a protective role against GC, while Roseburia, Family XI, and Eubacterium brachy group were associated with an increased risk. (Source: Frontiers in Microbiology)
Source: Frontiers in Microbiology - April 2, 2024 Category: Microbiology Source Type: research
Novel microbiota Mesosutterella faecium sp. nov. has a protective effect against inflammatory bowel disease
A novel Gram-negative, obligate anaerobe, non-motile, flagella-lacking, catalase- and oxidase-negative, coccobacilli-shaped bacterial strain designated AGMB02718T was isolated from swine feces. The 16S rRNA gene analysis indicated that strain AGMB02718T belonged to the genus Mesosutterella with the highest similarity to M. multiformis 4NBBH2T (= DSM 106860T) (sequence similarity of 96.2%), forming a distinct phylogenetic lineage. Its growth occurred at 25–45°C (optimal 37°C) and in 0.5–1% NaCl (optimal 0.5%). Strain AGMB02718T was asaccharolytic and contained menaquinone 6 (MK-6) and methylmenaquinone 6 (MMK-6) as th...
Source: Frontiers in Microbiology - April 2, 2024 Category: Microbiology Source Type: research
Resurgence of respiratory syncytial virus with dominance of RSV-B during the 2022 –2023 season
ConclusionThis study revealed a strong resurgence of RSV in the autumn of 2022 after the lifting of anti-COVID-19 measures, the leading role of RSV as a causative agent of serious respiratory illnesses in early childhood, and relatively low genetic diversity in circulating RSV strains. (Source: Frontiers in Microbiology)
Source: Frontiers in Microbiology - April 2, 2024 Category: Microbiology Source Type: research
Genome-based approach to evaluate the metabolic potentials and exopolysaccharides production of Bacillus paralicheniformis CamBx3 isolated from a Chilean hot spring
In the present study, a thermophilic strain designated CamBx3 was isolated from the Campanario hot spring, Chile. Based on 16S rRNA gene sequence, phylogenomic, and average nucleotide identity analysis the strain CamBx3 was identified as Bacillus paralicheniformis. Genome analysis of B. paralicheniformis CamBx3 revealed the presence of genes related to heat tolerance, exopolysaccharides (EPS), dissimilatory nitrate reduction, and assimilatory sulfate reduction. The pangenome analysis of strain CamBx3 with eight Bacillus spp. resulted in 26,562 gene clusters, 7,002 shell genes, and 19,484 cloud genes. The EPS produced by B....
Source: Frontiers in Microbiology - April 2, 2024 Category: Microbiology Source Type: research
Roles of gut microbiota in androgenetic alopecia: insights from Mendelian randomization analysis
ConclusionThe MR study has established a link between specific gut microbiota and AGA, offering evidence for the identification of more precisely targeted probiotics. This discovery has the potential to aid in the prevention, control, and reversal of AGA progression. (Source: Frontiers in Microbiology)
Source: Frontiers in Microbiology - April 2, 2024 Category: Microbiology Source Type: research
Scenario-based assessment of fecal pathogen sources affecting bathing water quality: novel treatment options to reduce norovirus and Campylobacter infection risks
In this study, quantitative microbial risk assessment (QMRA) tools were used to evaluate the hygienic impact of sewage effluents and runoff water from municipalities and animal farms on surface and bathing waters. The human-specific microbial source tracking (MST) marker HF183 was used to evaluate the dilution of fecal pathogens originating from the sewage effluent discharge to the downstream watershed. As novel risk management options, the efficiency of UV-LED disinfection and wetland treatment as well as biochar filtration was tested on-site for the contamination sources. According to the dilution pattern of the MST mark...
Source: Frontiers in Microbiology - April 2, 2024 Category: Microbiology Source Type: research
Induced Burkholderia prophages detected from the hemoculture: a biomarker for Burkholderia pseudomallei infection
Bacteriophages (phages), viruses that infect bacteria, are found in abundance not only in the environment but also in the human body. The use of phages for the diagnosis of melioidosis, a tropical infectious disease caused by Burkholderia pseudomallei, is emerging as a promising novel approach, but our understanding of conditions under which Burkholderia prophages can be induced remains limited. Here, we first demonstrated the isolation of Burkholderia phages from the hemocultures of melioidosis patients. The B. pseudomallei-positive hemoculture bottles were filtered to remove bacteria, and then phages were isolated and pu...
Source: Frontiers in Microbiology - April 2, 2024 Category: Microbiology Source Type: research
A red seaweed kappaphycus alvarezii-based biostimulant (AgroGain ®) improves the growth of Zea mays and impacts agricultural sustainability by beneficially priming rhizosphere soil microbial community
In this study, foliar sprays of biostimulant (AgroGain/LBS6) prepared from the cultivated, tropical red seaweed Kappaphycus alvarezii increased the phenotypic growth of Zea mays in terms of greater leaf area, total plant height, and shoot fresh and dry weights. In addition, LBS6 improved the accumulation of chlorophyll a and b, total carotenoids, total soluble sugars, amino acids, flavonoids, and phenolics in the treated plants. LBS6 applications also improved the total bacterial and fungal count in rhizospheric soil. The V3-V4 region of 16S rRNA gene from the soil metagenome was analyzed to study the abundance of bacteria...
Source: Frontiers in Microbiology - April 2, 2024 Category: Microbiology Source Type: research
Effects of dietary energy levels on microorganisms and short-chain fatty acids of rumen and tight junction proteins in Honghe Yellow cattle
This study was conducted to investigate the effects of dietary energy levels on microorganisms and short-chain fatty acids (SCFAs) of rumen and the expression of tight junction proteins in Honghe Yellow cattle. A total of fifteen male Honghe Yellow cattle were randomly divided into three treatments (five replicates per treatment), consisting of formulated energy concentrations of 5.90 MJ/kg (high-energy diet, group H), 5.60 MJ/kg (medium-energy diet, group M) and 5.30 MJ/kg (low-energy diet, group L). The results showed that compared with group H, the expression of Claudin-1 in rumen epithelium of groups M and L was ...
Source: Frontiers in Microbiology - April 2, 2024 Category: Microbiology Source Type: research
Relationships among bacterial cell size, diversity, and taxonomy in rumen
In this study, we used polyvinylidene difluoride filters with pore sizes of 300, 120, 80, 40, 20, 8, 6, 2.1, and 0.6 μm. Bacterial suspensions were successively passed through these filters for the analysis of microbial population distribution using 16S rRNA gene sequences.ResultsWe found that bacteria from the different pore sizes were clustered into four branches (> 120 μm, 40–120 μm, 6–20 μm, 20–40 μm, and < 0.6 μm), indicating that size fractionation had effects on enriching specific groups but could not effectively separate dominant groups by cell size alone. The species of unclassifi...
Source: Frontiers in Microbiology - April 2, 2024 Category: Microbiology Source Type: research
Foundation species stabilize an alternative eutrophic state in nutrient-disturbed ponds via selection on microbial community
In this study, we used whole-pond experimental manipulations to investigate the impacts of disturbance by nutrient additions in the presence and absence of two foundation species: Dreissena polymorpha (a freshwater mussel) and Myriophyllum spicatum (a macrophyte). We tracked how nutrient additions to ponds changed the prokaryotic and eukaryotic communities, using 16S, 18S, and COI amplicon sequencing. The nutrient disturbance and foundation species imposed strong selection on the prokaryotic communities, but not on the microbial eukaryotic communities. The prokaryotic communities changed increasingly over time as the nutri...
Source: Frontiers in Microbiology - April 2, 2024 Category: Microbiology Source Type: research
Epiphytic microbiota source stimulates the fermentation profile and bacterial community of alfalfa-corn mixed silage
The epiphytic microbiota source on plants plays a crucial role in the production of high-quality silage. To gain a better understanding of its contribution, the microbiota of alfalfa (M1C0), corn (M0C1) and the resulting mixture (M1C1) was applied in alfalfa-corn mixed silage production system. M1C0 decreased ammonia-N levels in terms of total nitrogen (57.59–118.23 g/kg TN) and pH (3.59–4.40) values (p < 0.01), which increased lactic acid (33.73–61.89 g/kg DM) content (p < 0.01). Consequently, this resulted in higher residual water-soluble carbohydrate (29.13–41.76 g/kg DM) and crude protein (1...
Source: Frontiers in Microbiology - April 2, 2024 Category: Microbiology Source Type: research
Editorial: Ecological interactions between mosquitoes and their microbiota: implications for pathogen transmission
(Source: Frontiers in Microbiology)
Source: Frontiers in Microbiology - March 28, 2024 Category: Microbiology Source Type: research
