Detection and characterization of eravacycline heteroresistance in clinical bacterial isolates
This study aimed to investigate ERV HR prevalence among common clinical isolates and further characterize ERV HR in carbapenem-resistant Klebsiella pneumoniae (CRKP). A total of 280 clinical pathogens from two centers were selected for HR and analyzed using population analysis profiling (PAP) and modified E-tests. The PAP assay revealed an overall ERV HR prevalence of 0.7% (2/280), with intermediate heterogeneity observed in 24.3% (68/280) of strains. The proportion of heteroresistant strains was 18.3% according to modified E-test results. A time-killing assay demonstrated that CRKP CFU increased significantly after 10 h...
Source: Frontiers in Microbiology - March 27, 2024 Category: Microbiology Source Type: research
Association between gut microbiota and autoimmune cholestatic liver disease, a Mendelian randomization study
ConclusionThis study found a causal relationship between gut microbiota and ACLD. This may provide valuable insights into gut microbiota-mediated pathogenesis of ACLD. It is necessary to conduct a large-sample randomized controlled trial (RCT) at a later stage to validate the associated role of the relevant gut microbiota in the risk of ACLD development and to explore the associated mechanisms. (Source: Frontiers in Microbiology)
Source: Frontiers in Microbiology - March 27, 2024 Category: Microbiology Source Type: research
Lacticaseibacillusparacasei BNCC345679 revolutionizes DSS-induced colitis and modulates gut microbiota
The gut microbiota plays an important role in the disease progression of inflammatory bowel disease. Although probiotics are effective against IBD, not many studies have investigated their effects on gut microbiota composition and immunomodulation in mouse colitis models. Our study aimed at the therapeutic effects of Lacticaseibacillus paracasei BNCC345679 for the first time and explored its impact on gut microbiome dysbiosis, inflammatory cytokines, related miRNAs, VCAM-1, oxidative stress, intestinal integrity, and mucus barrier. We found that oral intervention of L. paracasei BNCC345679 affects recovering beneficial mic...
Source: Frontiers in Microbiology - March 27, 2024 Category: Microbiology Source Type: research
Electroacupuncture regulates gut microbiota to reduce depressive-like behavior in rats
ConclusionThe antidepressant effect of EA at ST36 and ST25 is related to regulating intestinal flora and the neurotransmitter system. Our study suggests that EA contributes to the improvement of depression, and gut microbiota may be one of the mechanisms of EA effect. (Source: Frontiers in Microbiology)
Source: Frontiers in Microbiology - March 27, 2024 Category: Microbiology Source Type: research
Ultrasonic-assisted extraction, anti-biofilm activity, and mechanism of action of Ku Shen (Sophorae Flavescentis Radix) extracts against Vibrio parahaemolyticus
The objective of this study is to optimize the ultrasonic-assisted extraction process of Ku Shen (Sophorae Flavescentis Radix) extracts (KSE) against Vibrio parahaemolyticus and explore their anti-biofilm activity and mechanism of action. The ultrasonic-assisted extraction process of KSE optimized by single factor experiment, Box–Behnken design and response surface methodology was as follows: 93% ethanol as solvent, liquid/material ratio of 30 mL/g, ultrasonic power of 500 W, extraction temperature of 80°C and time of 30 min. Under these conditions, the diameter of inhibition circle of KSE was 15.60 ± 0.17 ...
Source: Frontiers in Microbiology - March 26, 2024 Category: Microbiology Source Type: research
Baltic Sea coastal sediment-bound eukaryotes have increased year-round activities under predicted climate change related warming
In this study, sediments were sampled from a heated (average 5°C increase over the whole year for over 50 years) and a control (contemporary conditions) Baltic Sea bay during four different seasons across a year. RNA transcript counts were then used to investigate eukaryotic community changes under long-term warming. The composition of active species in the heated and control bay sediment eukaryotic communities differed, which was mainly attributed to salinity and temperature. The family level RNA transcript alpha diversity in the heated bay was higher during May but lower in November, compared with the control bay, sugge...
Source: Frontiers in Microbiology - March 26, 2024 Category: Microbiology Source Type: research
Intestinal melatonin levels and gut microbiota homeostasis are independent of the pineal gland in pigs
ConclusionWe demonstrated that synthesis of MEL in the GIT is independent of the pineal gland. PINX had no influence on intestinal MEL level and microbiota composition in pigs, while exogenous MEL alters the structure of the gut microbiota. (Source: Frontiers in Microbiology)
Source: Frontiers in Microbiology - March 26, 2024 Category: Microbiology Source Type: research
Common mycorrhizal network: the predominant socialist and capitalist responses of possible plant –plant and plant–microbe interactions for sustainable agriculture
Plants engage in a variety of interactions, including sharing nutrients through common mycorrhizal networks (CMNs), which are facilitated by arbuscular mycorrhizal fungi (AMF). These networks can promote the establishment, growth, and distribution of limited nutrients that are important for plant growth, which in turn benefits the entire network of plants. Interactions between plants and microbes in the rhizosphere are complex and can either be socialist or capitalist in nature, and the knowledge of these interactions is equally important for the progress of sustainable agricultural practice. In the socialist network, reso...
Source: Frontiers in Microbiology - March 26, 2024 Category: Microbiology Source Type: research
Identification of a novel xanthan-binding module of a multi-modular Cohnella sp. xanthanase
A new strain of xanthan-degrading bacteria identified as Cohnella sp. has been isolated from a xanthan thickener for food production. The strain was able to utilize xanthan as the only carbon source and to reduce the viscosity of xanthan-containing medium during cultivation. Comparative analysis of the secretomes of Cohnella sp. after growth on different media led to the identification of a xanthanase designated as CspXan9, which was isolated after recombinant production in Escherichia coli. CspXan9 could efficiently degrade the β-1,4-glucan backbone of xanthan after previous removal of pyruvylated mannose residues from t...
Source: Frontiers in Microbiology - March 26, 2024 Category: Microbiology Source Type: research
Effects of Bacillus coagulans TBC169 on gut microbiota and metabolites in gynecological laparoscopy patients
ConclusionThis study demonstrated that consuming Bacillus coagulans TBC169 after gynecological laparoscopic surgery might inhibit the proliferation of harmful Enterobacteriaceae; mainly influence 2 pathways including D-glutamine and D-glutamate metabolism, and arginine biosynthesis; and regulate metabolites related to immunity and intestinal motility; which can help regulate immune function, maintain intestinal balance, promote intestinal peristalsis, and thus accelerate the recovery of intestinal function. (Source: Frontiers in Microbiology)
Source: Frontiers in Microbiology - March 26, 2024 Category: Microbiology Source Type: research
Genomic and biological control of Sclerotinia sclerotiorum using an extracellular extract from Bacillus velezensis 20507
This study provides new resources for the biological control of S. Sclerotiorum in soybeans and a theoretical basis for further clarification of the mechanism of action of B. velezensis 20507. (Source: Frontiers in Microbiology)
Source: Frontiers in Microbiology - March 26, 2024 Category: Microbiology Source Type: research
SOS genes are rapidly induced while translesion synthesis polymerase activity is temporally regulated
The DNA damage inducible SOS response in bacteria serves to increase survival of the species at the cost of mutagenesis. The SOS response first initiates error-free repair followed by error-prone repair. Here, we have employed a multi-omics approach to elucidate the temporal coordination of the SOS response. Escherichia coli was grown in batch cultivation in bioreactors to ensure highly controlled conditions, and a low dose of the antibiotic ciprofloxacin was used to activate the SOS response while avoiding extensive cell death. Our results show that expression of genes involved in error-free and error-prone repair were bo...
Source: Frontiers in Microbiology - March 26, 2024 Category: Microbiology Source Type: research
Metagenomics reveals the response of desert steppe microbial communities and carbon-nitrogen cycling functional genes to nitrogen deposition
This study provides new insights into the effects of N deposition on soil microbial communities and functions in desert steppe and a better understanding of the ecological consequences of anthropogenic N addition. (Source: Frontiers in Microbiology)
Source: Frontiers in Microbiology - March 26, 2024 Category: Microbiology Source Type: research
Tick-borne Rickettsia, Anaplasma, Theileria, and enzootic nasal tumor virus in ruminant, PET, and poultry animals in Pakistan
ConclusionThis study reveals that there is a considerably wider range of TBPs held in Pakistan that take in various contagious zoonotic pathogens than was previously thought. This information advances TBP epidemiology and will contribute to upgrade future control measure. (Source: Frontiers in Microbiology)
Source: Frontiers in Microbiology - March 26, 2024 Category: Microbiology Source Type: research
Microbiota profiling reveals alteration of gut microbial neurotransmitters in a mouse model of autism-associated 16p11.2 microduplication
The gut-brain axis is evident in modulating neuropsychiatric diseases including autism spectrum disorder (ASD). Chromosomal 16p11.2 microduplication 16p11.2dp/+ is among the most prevalent genetic copy number variations (CNV) linked with ASD. However, the implications of gut microbiota status underlying the development of ASD-like impairments induced by 16p11.2dp/+ remains unclear. To address this, we initially investigated a mouse model of 16p11.2dp/+, which exhibits social novelty deficit and repetitive behavior characteristic of ASD. Subsequently, we conducted a comparative analysis of the gut microbial community and me...
Source: Frontiers in Microbiology - March 26, 2024 Category: Microbiology Source Type: research
