Construction of cellulose-degrading microbial consortium and evaluation of their ability to degrade spent mushroom substrate
In this study, four strains of cellulose-degrading bacteria were screened, and F16, F, and F7 were identified as B. amyloliquefaciens, PX1 identified as B. velezensis. At the same time, two groups of cellulose efficient degrading microbial consortiums (PX1 + F7 and F16 + F) were successfully constructed. When SMS was used as the sole carbon source, their carboxymethyl cellulase (CMCase) activities were 225.16 and 156.63 U/mL, respectively, and the filter paper enzyme (FPase) activities were 1.91 and 1.64 U/mL, respectively. PX1 + F7 had the highest degradation rate of hemicellulose and lignin, reaching 52.96% and 52.13%, r...
Source: Frontiers in Microbiology - March 14, 2024 Category: Microbiology Source Type: research
Soil nitrogen availability and microbial carbon use efficiency are dependent more on chemical fertilization than winter drought in a maize –soybean rotation system
Soil nitrogen (N) availability is one of the limiting factors of crop productivity, and it is strongly influenced by global change and agricultural management practices. However, very few studies have assessed how the winter drought affected soil N availability during the subsequent growing season under chemical fertilization. We conducted a field investigation involving snow removal to simulate winter drought conditions in a Mollisol cropland in Northeast China as part of a 6-year fertilization experiment, and we examined soil physicochemical properties, microbial characteristics, and N availability. Our results demonstra...
Source: Frontiers in Microbiology - March 14, 2024 Category: Microbiology Source Type: research
SARS-CoV-2 mutant spectra as variant of concern nurseries: endless variation?
DiscussionWe evaluate possible interpretations of these findings, and reviewed precedents for rapid selection of genomes with multiple mutations in RNA viruses. These considerations suggest that intra-host evolution may be sufficient to generate minority sequences which are closely related to sequences typical of other clades. The results provide a model for the origin of variants of concern during epidemic spread─in particular Omicron lineages─that does not require prolonged infection, involvement of immunocompromised individuals, or participation of intermediate, non-human hosts. (Source: Frontiers in Microbiology)
Source: Frontiers in Microbiology - March 14, 2024 Category: Microbiology Source Type: research
Microbiome markers in HPV-positive and HPV-negative women of reproductive age with ASCUS and SIL determined by V4 region of 16S rRNA gene sequencing
DiscussionHPV16 is the most prevalent HPV type. We have not found any differences between diversity in studied groups, but several genus [like Prevotella (p-value = 0.026), Gardnerella (p-value = 0.003), Fannyhessea (p-value = 0.024)] more often occurred in HSIL group compared by NILM or LSIL regardless of HPV. We have found statistically significant difference in occurrence or proportion of bacterial genus in studied groups. We also identified that increasing of the ratio of Lactobacillus iners or age of patient lead to higher chance to HSIL, while increasing of the ratio of Lactobacillus crispatus lead to hig...
Source: Frontiers in Microbiology - March 14, 2024 Category: Microbiology Source Type: research
Comprehensive genome sequence analysis of Ralstonia solanacearum gd-2, a phylotype I sequevar 15 strain collected from a tobacco bacterial phytopathogen
In this study, we identified and sequenced the genome of R. solanacearum strain gd-2 isolated from tobacco.ResultsStrain gd-2 was identified as R. solanacearum species complex (RSSC) phylotype I sequevar 15 and exhibited strong pathogenicity to tobacco. The genome size of gd-2 was 5.93 Mb, including the chromosomes (3.83 Mb) and the megaplasmid (2.10 Mb). Gene prediction results showed that 3,434 and 1,640 genes were identified in the chromosomes and plasmids, respectively. Comparative genomic analysis showed that gd-2 exhibited high conservation with ten highly similar strain genomes and the differences between gd-2 and o...
Source: Frontiers in Microbiology - March 14, 2024 Category: Microbiology Source Type: research
Research reviews and prospects of gut microbiota in liver cirrhosis: a bibliometric analysis (2001 –2023)
This study is aiming to conduct a complete analysis of the knowledge structure and centers pertaining to gut microbiota in liver cirrhosis using bibliometric methods. Publications on gut microbiota and liver cirrhosis from 2001 to 2023 are sourced from the Web of Science Core Collection. For the bibliometric analysis, we employ VOSviewer, CiteSpace, and the R package “bibliometrix”.ResultsOur study encompasses a comprehensive collection of 3109 articles originating from 96 countries, with notable contributions from leading nations such as the United States and China. The quantity of publications concerning the gut micr...
Source: Frontiers in Microbiology - March 14, 2024 Category: Microbiology Source Type: research
Corrigendum: Active substances of myxobacteria against plant diseases and their action mechanisms
(Source: Frontiers in Microbiology)
Source: Frontiers in Microbiology - March 13, 2024 Category: Microbiology Source Type: research
A multiplex TaqMan real-time PCR assays for the rapid detection of mobile colistin resistance (mcr-1 to mcr-10) genes
ConclusionThe assay developed in this study has the advantages of high specificity, sensitivity, and reproducibility. It can be used to specifically detect drug-resistant clinical isolates carrying the mcr genes (mcr-1 to mcr-10), thus providing a better basis for clinical drug treatment and drug resistance research. (Source: Frontiers in Microbiology)
Source: Frontiers in Microbiology - March 13, 2024 Category: Microbiology Source Type: research
Beneficial effects of American ginseng (Panax quinquefolius L.) extract residue as a feed additive on production, health status, and gastrointestinal bacteria in sika deer (Cervus nippon)
This study comprehensively evaluated the effects of American ginseng residue as a potential feed additive on the production performance and gastrointestinal bacterial community in antler-bearing sika deer. The results indicated that ginseng residue was a suitable feed additive for improving production performance and health in sika deer. (Source: Frontiers in Microbiology)
Source: Frontiers in Microbiology - March 13, 2024 Category: Microbiology Source Type: research
Interactions between halotolerant nitrogen-fixing bacteria and arbuscular mycorrhizal fungi under saline stress
ConclusionThe impact of HNFB on the AMF community in apple rhizospheres is influenced by soil nitrogen levels. The study reveals how varying nitrogen levels mediate the relationship between exogenous HNFB, soil properties, and rhizosphere microbes. (Source: Frontiers in Microbiology)
Source: Frontiers in Microbiology - March 13, 2024 Category: Microbiology Source Type: research
Application of photoelectrochemical oxidation of wastewater used in the cooling tower water and its influence on microbial corrosion
DiscussionA metagenomics study revealed that bacteria present in the cooling tower water consists of 12% of Nitrospira genus and 22% of Fusobacterium genus. Conclusively, PEO serves as an effective method for treating wastewater, inhibiting microbial growth, degrading pollutants, and protecting metal from biocorrosion. (Source: Frontiers in Microbiology)
Source: Frontiers in Microbiology - March 13, 2024 Category: Microbiology Source Type: research
Corrigendum: Transcriptome reveals the role of the htpG gene in mediating antibiotic resistance through cell envelope modulation in Vibrio mimicus SCCF01
(Source: Frontiers in Microbiology)
Source: Frontiers in Microbiology - March 13, 2024 Category: Microbiology Source Type: research
Editorial: Next generation solutions for efficient management of phytopathogens
(Source: Frontiers in Microbiology)
Source: Frontiers in Microbiology - March 13, 2024 Category: Microbiology Source Type: research
Editorial: Women in microbial symbioses: 2022/2023
(Source: Frontiers in Microbiology)
Source: Frontiers in Microbiology - March 13, 2024 Category: Microbiology Source Type: research
The alternative coproporphyrinogen III oxidase (CgoN) catalyzes the oxygen-independent conversion of coproporphyrinogen III into coproporphyrin III
In this study, we report the bioinformatic-based identification of a gene called ytpQ, encoding a putative oxygen-independent counterpart, which we propose to term CgoN, from Priestia (Bacillus) megaterium. The recombinantly produced, purified, and monomeric YtpQ (CgoN) protein is shown to catalyze the oxygen-independent conversion of coproporphyrinogen III into coproporphyrin III. Minimal non-enzymatic conversion of coproporphyrinogen III was observed under the anaerobic test conditions employed in this study. FAD was identified as a cofactor, and menadione served as an artificial acceptor for the six abstracted electrons...
Source: Frontiers in Microbiology - March 13, 2024 Category: Microbiology Source Type: research
