Natronoglomus mannanivorans gen. nov., sp. nov., beta-1,4-mannan utilizing natronoarchaea from hypersaline soda lakes
Beta-mannans are insoluble plant polysaccharides with beta-1,4-linked mannose as the backbone. We used three forms of this polysaccharide, namely, pure mannan, glucomannan, and galactomannan, to enrich haloarchaea, which have the ability to utilize mannans for growth. Four mannan-utilizing strains obtained in pure cultures were closely related to each other on the level of the same species. Furthermore, another strain selected from the same habitats with a soluble beta-1,4-glucan (xyloglucan) was also able to grow with mannan. The phylogenomic analysis placed the isolates into a separate lineage of the new genus level with...
Source: Frontiers in Microbiology - March 12, 2024 Category: Microbiology Source Type: research
Protective potential of outer membrane vesicles derived from a virulent strain of Francisella tularensis
Francisella tularensis secretes tubular outer membrane vesicles (OMVs) that contain a number of immunoreactive proteins as well as virulence factors. We have reported previously that isolated Francisella OMVs enter macrophages, cumulate inside, and induce a strong pro-inflammatory response. In the current article, we present that OMVs treatment of macrophages also enhances phagocytosis of the bacteria and suppresses their intracellular replication. On the other hand, the subsequent infection with Francisella is able to revert to some extent the strong pro-inflammatory effect induced by OMVs in macrophages. Being derived fr...
Source: Frontiers in Microbiology - March 12, 2024 Category: Microbiology Source Type: research
Development and validation of a random forest algorithm for source attribution of animal and human Salmonella Typhimurium and monophasic variants of S. Typhimurium isolates in England and Wales utilising whole genome sequencing data
Source attribution has traditionally involved combining epidemiological data with different pathogen characterisation methods, including 7-gene multi locus sequence typing (MLST) or serotyping, however, these approaches have limited resolution. In contrast, whole genome sequencing data provide an overview of the whole genome that can be used by attribution algorithms. Here, we applied a random forest (RF) algorithm to predict the primary sources of human clinical Salmonella Typhimurium (S. Typhimurium) and monophasic variants (monophasic S. Typhimurium) isolates. To this end, we utilised single nucleotide polymorphism dive...
Source: Frontiers in Microbiology - March 12, 2024 Category: Microbiology Source Type: research
Biological characteristics of the bacteriophage LDT325 and its potential application against the plant pathogen Pseudomonas syringae
In this study, a P. syringae phage was isolated from soil samples. Based on morphological characteristics, bacteriophage vB_PsS_LDT325 belongs to the Siphoviridae family; it has an icosahedral head with a diameter of 53 ± 1 nm and nonretractable tails measuring 110 ± 1 nm. The latent period and burst size of the phage were 10 min and 17 plaque-forming units (PFU)/cell, respectively. Furthermore, an analysis of the biological traits showed that the optimal multiplicity of infection (MOI) of the phage was 0.01. When the temperature exceeded 60 ℃, the phage titer began to decrease. The phage exhibited tolerance to a wide ...
Source: Frontiers in Microbiology - March 12, 2024 Category: Microbiology Source Type: research
Corrigendum: Isolation, identification, recombination analysis and pathogenicity experiment of a PRRSV recombinant strain in Sichuan Province, China
(Source: Frontiers in Microbiology)
Source: Frontiers in Microbiology - March 11, 2024 Category: Microbiology Source Type: research
Editorial: Host factors involved in viral infection
(Source: Frontiers in Microbiology)
Source: Frontiers in Microbiology - March 11, 2024 Category: Microbiology Source Type: research
Editorial: Cellular nanomotion as a new signature of life
(Source: Frontiers in Microbiology)
Source: Frontiers in Microbiology - March 11, 2024 Category: Microbiology Source Type: research
Metagenomic sequencing identified microbial species in the rumen and cecum microbiome responsible for niacin treatment and related to intramuscular fat content in finishing cattle
ConclusionThe results suggested that dietary manipulation, such as the supplementation of niacin, should be regarded as the effective and convenient way to improve IMF of castrated finishing steers by regulating rumen microbiome. (Source: Frontiers in Microbiology)
Source: Frontiers in Microbiology - March 11, 2024 Category: Microbiology Source Type: research
Microbial diversity in camel milk from Xinjiang, China as revealed by metataxonomic analysis
In this study, we obtained 20 samples of camel milk during spring and summer in Urumqi and Hami, Xinjiang, China. Single-molecule real-time sequencing technology was used to analyze the bacterial community composition. The results revealed that there were significant seasonal differences in the bacterial composition and diversity of camel milk. Overall, Epilithonimonas was the most abundant bacterial genus in our samples. Through the annotated genes inferred by PICRUSt2 were mapped against KEGG database. Non-parametric analysis of the bacterial community prediction function revealed a strong bacterial interdependence with ...
Source: Frontiers in Microbiology - March 11, 2024 Category: Microbiology Source Type: research
Epidemiological investigation and pathogenicity analysis of waterfowl astroviruses in some areas of China
In this study, we collected 1546 waterfowl samples from 13 provinces in China for epidemiological investigation. The results showed that 260 samples (16.8%) were positive. Four species of astrovirus were detected in 13 provinces except Fujian province. Among the four sites tested, the highest positive rates were found in farms and slaughterhouses. Cross-host and mixed infection were observed in four species of waterfowl astroviruses. The whole genome of 17 isolates was sequenced and compared with published sequences. Genetic evolution and homology analysis showed that the isolated strains had high similarity to their refer...
Source: Frontiers in Microbiology - March 11, 2024 Category: Microbiology Source Type: research
Biological control potential of worrisome wheat blast disease by the seed endophytic bacilli
This study aimed to identify potential bacterial isolates from rice and wheat seeds with inhibitory effects against MoT. In dual culture and seedling assays, three bacterial isolates (BTS-3, BTS-4, and BTLK6A) demonstrated effective suppression of MoT growth and reduced wheat blast severity when artificially inoculated at the seedling stage. Genome phylogeny identified these isolates as Bacillus subtilis (BTS-3) and B. velezensis (BTS-4 and BTLK6A). Whole-genome analysis revealed the presence of genes responsible for controlling MoT through antimicrobial defense, antioxidant defense, cell wall degradation, and induced syst...
Source: Frontiers in Microbiology - March 11, 2024 Category: Microbiology Source Type: research
Marine bacteriophages disturb the associated microbiota of Aurelia aurita with a recoverable effect on host morphology
The concept of the metaorganism describes a multicellular host and its diverse microbial community, which form one biological unit with a combined genetic repertoire that significantly influences health and survival of the host. The present study delved into the emerging field of bacteriophage research within metaorganisms, focusing on the moon jellyfish Aurelia aurita as a model organism. The previously isolated Pseudomonas phage BSwM KMM1 and Citrobacter phages BSwM KMM2 – KMM4 demonstrated potent infectivity on bacteria present in the A. aurita-associated microbiota. In a host-fitness experiment, Baltic Sea subpopulat...
Source: Frontiers in Microbiology - March 11, 2024 Category: Microbiology Source Type: research
Unraveling the shift in bacterial communities profile grown in sediments co-contaminated with chlorolignin waste of pulp-paper mill by metagenomics approach
This study unveils key insights into contaminant characteristics and shifts in bacterial communities within contaminated environments. It highlights the potential for developing efficient bioremediation techniques to restore ecological balance in pulp-paper mill waste-polluted areas, stressing the importance of identifying a significant percentage of unclassified genera and species to explore novel genes. (Source: Frontiers in Microbiology)
Source: Frontiers in Microbiology - March 11, 2024 Category: Microbiology Source Type: research
Fe(III) bioreduction kinetics in anaerobic batch and continuous stirred tank reactors with acidophilic bacteria relevant for bioleaching of limonitic laterites
This study focused on the Fe(III) bioreduction which is a relevant reaction of this process. In the first step, biomass growth was sustained by aerobic bio-oxidation of elemental sulfur. In the second step, the biomass anaerobically reduced Fe(III) to Fe(II). The last step, which is not in the scope of this study, was the reduction of limonites and the dissolution of metals. This study aimed at assessing the Fe(III) bioreduction rate at 35°C with a microbial consortium composed predominantly of Sulfobacillus (Sb.) species as the iron reducers and Acidithiobacillus (At.) caldus. It evaluated the influence of the biomass co...
Source: Frontiers in Microbiology - March 11, 2024 Category: Microbiology Source Type: research
Multi-omics approaches to understand pathogenicity during potato early blight disease caused by Alternaria solani
In this study, we focused on the composition and infection characteristics of early blight pathogens in Yunnan Province and screened candidate pathogenesis-related pathways and genes. We isolated 85 strains of Alternaria sp. fungi from typical early blight spots in three potato-growing regions in Yunnan Province from 2018 to 2022, and identified 35 strains of Alternaria solani and 50 strains of Alternaria alternata by morphological characterization and ITS sequence comparison, which were identified as the main and conditional pathogens causing early blight in potato, respectively. Scanning electron microscope analysis conf...
Source: Frontiers in Microbiology - March 11, 2024 Category: Microbiology Source Type: research
