A pool of peptides extracted from wheat bud chromatin inhibits tumor cell growth by causing defective DNA synthesis
Conclusion:
The data reported here show that the antiproliferative effect exhibited by these chromatin peptides results from their ability to induce genomic stress during DNA synthesis. This effect seems to be S-phase specific since surviving cells are able to progress through their normal cell cycle when the peptide fraction is removed from the culture medium. It is likely that the subsequent apoptosis is a consequence of the failed attempt of the tumour cells to repair the DNA damage induced by the peptides. (Source: Cell Division)
Source: Cell Division - August 6, 2013 Category: Cytology Authors: Loretta MancinelliTeresa SeccaPaula De AngelisFrancesco ManciniMatteo MarchesiniCristiano MarinelliLanfranco BarberiniFrancesco Grignani Source Type: research
Silencing CDK4 radiosensitizes breast cancer cells by promoting apoptosis
Conclusion:
Based on our data we conclude that knockdown of CDK4 activity sensitizes breast cancer cells to radiation by activating apoptosis pathways. (Source: Cell Division)
Source: Cell Division - July 25, 2013 Category: Cytology Authors: Katie HagenXiangbin ZengMi-Young LeeShannon Tucker KahnMary Harrison PitnerSandra ZakyYuan LiuRuth O¿ReganXingming DengHarold Saavedra Source Type: research
Mutually dependent degradation of Ama1p and Cdc20p terminates APC/C ubiquitin ligase activity at the completion of meiotic development in yeast
Conclusions:
Unlike the cyclical nature of mitotic cell division, meiosis is a linear pathway leading to the production of quiescent spores. This raises the question of how the APC/C is reset prior to spore germination. This and a previous study revealed that Cdc20p and Ama1p direct each others degradation via APC/C-dependent degradation. These findings suggest a model that the APC/C is inactivated by mutual degradation of the activators. In addition, these results support a model in which Ama1p and Cdc20p relocate to the substrate address within the APC/C cavity prior to degradation. (Source: Cell Division)
Source: Cell Division - July 1, 2013 Category: Cytology Authors: Grace TanRebecca LewandowskiMichael MalloryRandy StrichKatrina Cooper Source Type: research
To divide or not to divide: revisiting liver regeneration
The liver has a remarkable capacity to regenerate. Even with surgical removal (partial hepatectomy) of 70% of liver mass, the remnant tissue grows to recover the original mass and functions. Liver regeneration after partial hepatectomy has been studied extensively since the 19th century, establishing the long-standing model that hepatocytes, which account for most of the liver weight, proliferate to recover the original mass of the liver. The basis of this model is the fact that almost all hepatocytes undergo S phase, as shown by the incorporation of radioactive nucleotides during liver regeneration. However, DNA replicati...
Source: Cell Division - June 20, 2013 Category: Cytology Authors: Yuichiro MiyaokaAtsushi Miyajima Source Type: research
Cell cycle-dependent localization of CHK2 at centrosomes during mitosis
Conclusion:
Our findings demonstrate that a subpopulation of CHK2 localizes at the centrosomes in mitotic cells but not in interphase. These results are consistent with previous reports supporting a role for CHK2 in the bipolar spindle formation and the timely progression of mitosis. (Source: Cell Division)
Source: Cell Division - May 16, 2013 Category: Cytology Authors: Guillaume ChouinardIsabelle ClémentJulie LafontaineFrancis RodierEstelle Schmitt Source Type: research
A 2D/3D image analysis system to track fluorescently labeled structures in rod-shaped cells: application to measure spindle pole asymmetry during mitosis
Conclusions:
"RodCell" is freely available to the community as a package of several ImageJ plugins to simultaneously analyze the behavior of a large number of rod-shaped cells in an extensive manner. The integration of different image-processing techniques in a single package, as well as the development of novel algorithms does not only allow to speed up the analysis with respect to the usage of existing tools, but also accounts for higher accuracy. Its utility was demonstrated on both 2D and 3D static and dynamic images to study the septation initiation network of the yeast Schizosaccharomyces pombe. More generally, it ca...
Source: Cell Division - April 27, 2013 Category: Cytology Authors: Daniel SchmitterPaulina WachowiczDaniel SageAnastasia ChasapiIoannis XenariosViesturs SimanisMichael Unser Source Type: research
Proteolysis of Xenopus Cip-type CDK inhibitor, p16Xic2, is regulated by PCNA binding and CDK2 phosphorylation
Conclusions:
During interphase, Xic2 is targeted for DNA- and PCNA-dependent proteolysis that is negatively regulated by CDK2 phosphorylation. During a response to DNA damage, Xic2 may be alternatively regulated by phosphorylation by a caffeine-sensitive kinase. Our studies suggest that the three types of Xenopus CDK inhibitors, Xic1, Xic2, and Xic3 appear to be uniquely regulated which may reflect their specialized roles during cell division or early development in the frog. (Source: Cell Division)
Source: Cell Division - April 22, 2013 Category: Cytology Authors: Xi-Ning ZhuDong KimHorng-Ru LinVarija BudhavarapuHerbert RosenbaumPaul MuellerP Yew Source Type: research
Rad53 homologue forkhead-associated kinase A (FhkA) and Ca2+-binding protein 4a (CBP4a) are nucleolar proteins that differentially redistribute during mitosis in Dictyostelium
Background:
During mitosis most nucleolar proteins redistribute to other locales providing an opportunity to study the relationship between nucleolar protein localization and function. Dictyostelium is a model organism for the study of several fundamental biological processes and human diseases but only two nucleolar proteins have been studied during mitosis: NumA1 and Snf12. Both of them are linked to the cell cycle. To acquire a better understanding of nucleolar protein localization and dynamics in Dictyostelium we studied the nucleolar localization of two additional proteins during mitosis: Snf12-linked forkhead-associa...
Source: Cell Division - April 12, 2013 Category: Cytology Authors: Andrew CatalanoDanton O¿Day Source Type: research
The novel BTB-kelch protein, KBTBD8, is located in the Golgi apparatus and translocates to the spindle apparatus during mitosis
In conclusion, KBTBD8 is a new member of the BTB-kelch superfamily that is located in the Golgi apparatus and translocates to the spindle apparatus during mitosis. (Source: Cell Division)
Source: Cell Division - April 11, 2013 Category: Cytology Authors: Sandra LührigSusanne KolbNadine MelliesJessica Nolte Source Type: research
Ndc80 Loop as a protein-protein interaction motif
Our understanding of the structure and function of kinetochores has advanced dramatically over the past 10 years, yet how the plus end of spindle microtubules interacts with the kinetochore and establishes amphitelic attachment for proper sister chromatid segregation remains unresolved. However, several recent reports from different organisms have shed new light on this issue. A key player in microtubule-kinetochore interaction is the conserved Ndc80 outer kinetochore complex. In both yeast and human cells in particular, a ubiquitous internal ‘loop’ found in the Ndc80 molecule interrupting its C-terminal coiled-coil d...
Source: Cell Division - March 15, 2013 Category: Cytology Authors: Ngang Heok TangTakashi Toda Source Type: research
Ndc80 Loop as a protein-protein interaction motif
Our understanding of the structure and function of kinetochores has advanced dramatically over the past 10 years, yet how the plus end of spindle microtubules interacts with the kinetochore and establishes amp... (Source: Cell Division)
Source: Cell Division - March 15, 2013 Category: Cytology Authors: Ngang Heok Tang and Takashi Toda Source Type: research
The role of ß-adrenergic receptor signaling in the proliferation of hemangioma-derived endothelial cells
Conclusions:
We have demonstrated that the activation of the beta-ARs in the ERK pathway may be important mechanisms in promoting HemEC growth. Furthermore, stimulation of the beta-AR may transactivate VEGFR-2 signaling and further increase HemEC proliferation. (Source: Cell Division)
Source: Cell Division - January 3, 2013 Category: Cytology Authors: Yi JiSiyuan ChenKai LiXianmin XiaoShan ZhengTing Xu Source Type: research
The role of beta-adrenergic receptor signaling in the proliferation of hemangioma-derived endothelial cells
Conclusions:
We have demonstrated that the activation of the beta-ARs in the ERK pathway may be important mechanisms in promoting HemEC growth. Furthermore, stimulation of the beta-AR may transactivate VEGFR-2 signaling and further increase HemEC proliferation. (Source: Cell Division)
Source: Cell Division - January 3, 2013 Category: Cytology Authors: Yi JiSiyuan ChenKai LiXianmin XiaoShan ZhengTing Xu Source Type: research
Retraction Note: Role of senescence and mitotic catastrophe in cancer therapy
(Source: Cell Division)
Source: Cell Division - May 15, 2012 Category: Cytology Authors: Richa Singh, Jasmine George and Yogeshwer Shukla Source Type: research
Obituary: Arun Fotedar
(Source: Cell Division)
Source: Cell Division - October 2, 2007 Category: Cytology Authors: Rati Fotedar and Robert L Margolis Source Type: research
