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Quantitative Assessment of the Post-Translational Modifications of Human Serum Albumin by Dimethyl Trisulfide
This study explored the post-translational modifications of human serum albumin using DMTS. Quantitative assessments were conducted on mixed disulfides of mercaptoalbumin with mercaptomethane (Alb-SS-CH3) and albumin hydropersulfide (Alb-SSH) as post-translationally modified species. The hydropersulfide group was alkylated with iodoacetamide, resulting in the formation of an albumin-mercaptoacetamide mixed disulfide. The mixed disulfides were subsequently reduced with tris(2-carboxyethyl)phosphine, and the liberated mercaptomethane and mercaptoacetamide were fluorescently labeled with 4-fluoro-7-sulfamoylbenzofurazan (ABD-...
Source: Biological and Pharmaceutical Bulletin - December 26, 2023 Category: Drugs & Pharmacology Authors: Ichiro Koshiishi Seiya Nagai Yasushi Yuzawa Yuta Takigawa Source Type: research

Quantitative Assessment of the Post-Translational Modifications of Human Serum Albumin by Dimethyl Trisulfide
This study explored the post-translational modifications of human serum albumin using DMTS. Quantitative assessments were conducted on mixed disulfides of mercaptoalbumin with mercaptomethane (Alb-SS-CH3) and albumin hydropersulfide (Alb-SSH) as post-translationally modified species. The hydropersulfide group was alkylated with iodoacetamide, resulting in the formation of an albumin-mercaptoacetamide mixed disulfide. The mixed disulfides were subsequently reduced with tris(2-carboxyethyl)phosphine, and the liberated mercaptomethane and mercaptoacetamide were fluorescently labeled with 4-fluoro-7-sulfamoylbenzofurazan (ABD-...
Source: Biological and Pharmaceutical Bulletin - December 26, 2023 Category: Drugs & Pharmacology Authors: Ichiro Koshiishi Seiya Nagai Yasushi Yuzawa Yuta Takigawa Source Type: research

Quantitative analysis of therapeutic antibody interactions with Fc γ receptors using high-speed atomic force microscopy
This study employed high-speed atomic force microscopy to quantitatively analyze the interactions between therapeutic antibodies and Fcγ receptors (FcγRs). Antibodies are essential components of the immune system and are integral to biopharmaceuticals. The focus of this study was on immunoglobulin G molecules, which are crucial for antigen binding via the Fab segments and cytotoxic functions through their Fc portions. We conducted real-time, label-free observations of the interactions of rituximab and mogamulizumab with the recombinant FcγRIIIa and FcγRIIa. The dwell times of FcγR binding were measured at the single-m...
Source: Biological and Pharmaceutical Bulletin - December 24, 2023 Category: Drugs & Pharmacology Authors: Saeko Yanaka Hiroki Watanabe Rina Yogo Mesayamas Kongsema Sachiko Kondo Hirokazu Yagi Takayuki Uchihashi Koichi Kato Source Type: research

Quantitative analysis of therapeutic antibody interactions with Fc γ receptors using high-speed atomic force microscopy
This study employed high-speed atomic force microscopy to quantitatively analyze the interactions between therapeutic antibodies and Fcγ receptors (FcγRs). Antibodies are essential components of the immune system and are integral to biopharmaceuticals. The focus of this study was on immunoglobulin G molecules, which are crucial for antigen binding via the Fab segments and cytotoxic functions through their Fc portions. We conducted real-time, label-free observations of the interactions of rituximab and mogamulizumab with the recombinant FcγRIIIa and FcγRIIa. The dwell times of FcγR binding were measured at the single-m...
Source: Biological and Pharmaceutical Bulletin - December 24, 2023 Category: Drugs & Pharmacology Authors: Saeko Yanaka Hiroki Watanabe Rina Yogo Mesayamas Kongsema Sachiko Kondo Hirokazu Yagi Takayuki Uchihashi Koichi Kato Source Type: research

Development of a New Jelly Coating Technology (Oral Jelly Coating) to Improve Prescribed Medication Adherence
Biol Pharm Bull. 2023 Dec 16. doi: 10.1248/bpb.b23-00625. Online ahead of print.ABSTRACTTablets are the most commonly prescribed dosage form for oral drug administration. Historically, improvement of medication adherence of tablets has been facilitated through, for example, the use of smaller tablets, distinctive shaped tablets and sugar-coated tablets. In addition, new formulation technologies such as orally disintegrating tablets (OD tablets), micro tablet-type granules, jellies, and film formulations are making it possible to create more easily ingested dosage forms. We have developed a new oral jelly coating formulatio...
Source: Biological and Pharmaceutical Bulletin - December 17, 2023 Category: Drugs & Pharmacology Authors: Junya Yamashita Shota Asai Hiroki Shingaki Masakane Hayakawa Source Type: research