Pseudomonas environmental strain produces a DegQ-derived and PDZ domain containing peptide with protease activity
This report provides the isolation and identification of a highly proteolytic fragment derived from DegQ produced by a Pseudomonas fluorescens environmental strain isolated from a frog carcass. Zymograms demonstrate that a 10 kDa protein mainly generates the total proteolytic activity of this strain, which is enhanced by the detergent SDS. Mass spectroscopy analysis revealed that the protein derived a couple of peptides, the ones showing the highest coverage belonging to DegQ. Interestingly, this small protein fragment contains a PDZ domain but no obvious residues indicating that it is a protease. Protein model analysis sh...
Source: Antonie van Leeuwenhoek - February 24, 2024 Category: Microbiology Authors: Francisco Vargas-Gasca Bernardo Franco Naur ú Idalia Vargas-Maya Marcos Vicente-G ómez Vianey Olmedo-Monfil Source Type: research
Function of Burkholderia pseudomallei RpoS and RpoN2 in bacterial invasion, intracellular survival, and multinucleated giant cell formation in mouse macrophage cell line
In this study, by using the mouse macrophage cell line RAW264.7 as a model of infection, the involvement of B. pseudomallei RpoS and RpoN2 in the invasion, intracellular survival leading to the reduction in multinucleated giant cell (MNGC) formation of RAW264.7 cell line were illustrated. We have demonstrated that the MNGC formation of RAW264.7 cell was dependent on a certain number of intracellular bacteria (at least 5 × 104). In addition, the same MNGC formation (15%) observed in RAW264.7 cells infected with either B. pseudomallei wild type with multiplicity of infection (MOI) 2 or RpoN2 mutant (∆rpoN2) with MOI 10 or...
Source: Antonie van Leeuwenhoek - February 23, 2024 Category: Microbiology Authors: Duong Thi Hong Diep Long Binh Vong Sumalee Tungpradabkul Source Type: research
Study of an Enterococcus faecium strain isolated from an artisanal Mexican cheese, whole-genome sequencing, comparative genomics, and bacteriocin expression
In this study, we performed whole-genome sequencing and comparative genomics of an Enterococcus faecium strain isolated from an artisanal Mexican Cotija cheese, namely QD-2. We found clear genomic differences between commensal and pathogenic strains, particularly in their carbohydrate metabolic pathways, resistance to vancomycin and other antibiotics, bacteriocin production, and bacteriophage and CRISPR content. Furthermore, a bacteriocin transcription analysis performed by RT-qPCR revealed that, at the end of the log phase, besides enterocins A and X, two putative bacteriocins not reported previously are also transcribed ...
Source: Antonie van Leeuwenhoek - February 23, 2024 Category: Microbiology Authors: Daniel Acero-Pimentel Diana I Romero-S ánchez Sac Nict é Fuentes-Curiel Maricarmen Quirasco Source Type: research
Function of Burkholderia pseudomallei RpoS and RpoN2 in bacterial invasion, intracellular survival, and multinucleated giant cell formation in mouse macrophage cell line
In this study, by using the mouse macrophage cell line RAW264.7 as a model of infection, the involvement of B. pseudomallei RpoS and RpoN2 in the invasion, intracellular survival leading to the reduction in multinucleated giant cell (MNGC) formation of RAW264.7 cell line were illustrated. We have demonstrated that the MNGC formation of RAW264.7 cell was dependent on a certain number of intracellular bacteria (at least 5 × 104). In addition, the same MNGC formation (15%) observed in RAW264.7 cells infected with either B. pseudomallei wild type with multiplicity of infection (MOI) 2 or RpoN2 mutant (∆rpoN2) with MOI 10 or...
Source: Antonie van Leeuwenhoek - February 23, 2024 Category: Microbiology Authors: Duong Thi Hong Diep Long Binh Vong Sumalee Tungpradabkul Source Type: research
Study of an Enterococcus faecium strain isolated from an artisanal Mexican cheese, whole-genome sequencing, comparative genomics, and bacteriocin expression
In this study, we performed whole-genome sequencing and comparative genomics of an Enterococcus faecium strain isolated from an artisanal Mexican Cotija cheese, namely QD-2. We found clear genomic differences between commensal and pathogenic strains, particularly in their carbohydrate metabolic pathways, resistance to vancomycin and other antibiotics, bacteriocin production, and bacteriophage and CRISPR content. Furthermore, a bacteriocin transcription analysis performed by RT-qPCR revealed that, at the end of the log phase, besides enterocins A and X, two putative bacteriocins not reported previously are also transcribed ...
Source: Antonie van Leeuwenhoek - February 23, 2024 Category: Microbiology Authors: Daniel Acero-Pimentel Diana I Romero-S ánchez Sac Nict é Fuentes-Curiel Maricarmen Quirasco Source Type: research
Similarity of Chinese and Pakistani oral microbiome
Antonie Van Leeuwenhoek. 2024 Feb 19;117(1):38. doi: 10.1007/s10482-024-01933-5.ABSTRACTOral microbiota is vital for human health and can be affected by various factors (i.e. diets, ethnicity). However, few studies have compared oral microbiota of individuals from different nationalities in the same environment. Here, we explored the assembly and interaction of oral microbial communities of Chinese and Pakistanis in one university. Firmicutes and Proteobacteria were the predominant microorganisms in the oral cavity of Chinese and Pakistanis. Streptococcus and Neisseria were the dominant genera of China, while Streptococcus...
Source: Antonie van Leeuwenhoek - February 19, 2024 Category: Microbiology Authors: Xiaofang Sun Binghua Han Qian Han Qiaoling Yu Sijie Wang Jie Feng Tianshu Feng Xiaoshan Li Shiheng Zhang Huan Li Source Type: research
Similarity of Chinese and Pakistani oral microbiome
Antonie Van Leeuwenhoek. 2024 Feb 19;117(1):38. doi: 10.1007/s10482-024-01933-5.ABSTRACTOral microbiota is vital for human health and can be affected by various factors (i.e. diets, ethnicity). However, few studies have compared oral microbiota of individuals from different nationalities in the same environment. Here, we explored the assembly and interaction of oral microbial communities of Chinese and Pakistanis in one university. Firmicutes and Proteobacteria were the predominant microorganisms in the oral cavity of Chinese and Pakistanis. Streptococcus and Neisseria were the dominant genera of China, while Streptococcus...
Source: Antonie van Leeuwenhoek - February 19, 2024 Category: Microbiology Authors: Xiaofang Sun Binghua Han Qian Han Qiaoling Yu Sijie Wang Jie Feng Tianshu Feng Xiaoshan Li Shiheng Zhang Huan Li Source Type: research
Similarity of Chinese and Pakistani oral microbiome
Antonie Van Leeuwenhoek. 2024 Feb 19;117(1):38. doi: 10.1007/s10482-024-01933-5.ABSTRACTOral microbiota is vital for human health and can be affected by various factors (i.e. diets, ethnicity). However, few studies have compared oral microbiota of individuals from different nationalities in the same environment. Here, we explored the assembly and interaction of oral microbial communities of Chinese and Pakistanis in one university. Firmicutes and Proteobacteria were the predominant microorganisms in the oral cavity of Chinese and Pakistanis. Streptococcus and Neisseria were the dominant genera of China, while Streptococcus...
Source: Antonie van Leeuwenhoek - February 19, 2024 Category: Microbiology Authors: Xiaofang Sun Binghua Han Qian Han Qiaoling Yu Sijie Wang Jie Feng Tianshu Feng Xiaoshan Li Shiheng Zhang Huan Li Source Type: research
Cell-free supernatants of probiotic consortia impede hyphal formation and disperse biofilms of vulvovaginal candidiasis causing Candida in an ex-vivo model
Antonie Van Leeuwenhoek. 2024 Feb 17;117(1):37. doi: 10.1007/s10482-024-01929-1.ABSTRACTVulvovaginal candidiasis is the second most common vaginal infection caused by drug-resistant Candida species that affects about 70-75% of reproductive age group women across the globe. As current-day antifungal drugs are ineffective against the biofilms formed by the drug-resistant Candida strains, several natural compounds and antagonistic microbes are being explored as alternative antifungal agents. In the present study, we investigated the anti-biofilm activity of Cell-Free Supernatant (CFS) extracted from the commercially available...
Source: Antonie van Leeuwenhoek - February 17, 2024 Category: Microbiology Authors: Sudaarsan Aruna Senthil Kumar Dhesiga Krishnan Sowndarya Jothipandiyan Ramyadevi Durai B Narayanan Vedha Hari Paramasivam Nithyanand Source Type: research
Clostridium lamae sp. nov., a novel bacterium isolated from the fresh feces of alpaca
Antonie Van Leeuwenhoek. 2024 Feb 17;117(1):36. doi: 10.1007/s10482-024-01931-7.ABSTRACTA novel Gram-positive, anaerobic, nonspore-forming, rod-shaped bacterium, designated strain NGMCC 1.200840 T, was isolated from the alpacas fresh feces. The taxonomic position of the novel strain was determined using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences revealed strain NGMCC 1.200840 T was a member of the genus Clostridium and closely related to Clostridium tertium DSM 2485 T (98.16% sequence similarity). Between strains NGMCC 1.200840 T and C. tertium DSM 2485 T, the average nucleotide identity ...
Source: Antonie van Leeuwenhoek - February 17, 2024 Category: Microbiology Authors: Xue Li Ming Li Weixiong Shi Xia Li Zhiguang Xiang Lei Su Source Type: research
Cell-free supernatants of probiotic consortia impede hyphal formation and disperse biofilms of vulvovaginal candidiasis causing Candida in an ex-vivo model
Antonie Van Leeuwenhoek. 2024 Feb 17;117(1):37. doi: 10.1007/s10482-024-01929-1.ABSTRACTVulvovaginal candidiasis is the second most common vaginal infection caused by drug-resistant Candida species that affects about 70-75% of reproductive age group women across the globe. As current-day antifungal drugs are ineffective against the biofilms formed by the drug-resistant Candida strains, several natural compounds and antagonistic microbes are being explored as alternative antifungal agents. In the present study, we investigated the anti-biofilm activity of Cell-Free Supernatant (CFS) extracted from the commercially available...
Source: Antonie van Leeuwenhoek - February 17, 2024 Category: Microbiology Authors: Sudaarsan Aruna Senthil Kumar Dhesiga Krishnan Sowndarya Jothipandiyan Ramyadevi Durai B Narayanan Vedha Hari Paramasivam Nithyanand Source Type: research
Clostridium lamae sp. nov., a novel bacterium isolated from the fresh feces of alpaca
Antonie Van Leeuwenhoek. 2024 Feb 17;117(1):36. doi: 10.1007/s10482-024-01931-7.ABSTRACTA novel Gram-positive, anaerobic, nonspore-forming, rod-shaped bacterium, designated strain NGMCC 1.200840 T, was isolated from the alpacas fresh feces. The taxonomic position of the novel strain was determined using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences revealed strain NGMCC 1.200840 T was a member of the genus Clostridium and closely related to Clostridium tertium DSM 2485 T (98.16% sequence similarity). Between strains NGMCC 1.200840 T and C. tertium DSM 2485 T, the average nucleotide identity ...
Source: Antonie van Leeuwenhoek - February 17, 2024 Category: Microbiology Authors: Xue Li Ming Li Weixiong Shi Xia Li Zhiguang Xiang Lei Su Source Type: research
Thiopseudomonas acetoxidans sp. nov., an aerobic acetic and butyric acids oxidizer isolated from anaerobic fermentation liquid of food waste
Antonie Van Leeuwenhoek. 2024 Feb 13;117(1):35. doi: 10.1007/s10482-024-01932-6.ABSTRACTA Gram-stain-negative, oxidase-negative, rod-shaped, motile, facultatively anaerobic bacterial strain, designated as CY1220T, was isolated from an anaerobic fermentation liquid of food waste treatment plant. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain CY1220T belongs to the genus Thiopseudomonas, with the highest sequence similarity to Thiopseudomonas alkaliphila B4199T (95.91%), followed by Thiopseudomonas denitrificans X2T (95.56%). The genomic DNA G + C content of strain CY1220T was 48.6 mol%. The...
Source: Antonie van Leeuwenhoek - February 13, 2024 Category: Microbiology Authors: Miaomiao An Ruina Liang Yanjuan Lu Xiaoxu Li Guozhu Zhao Source Type: research
Thiopseudomonas acetoxidans sp. nov., an aerobic acetic and butyric acids oxidizer isolated from anaerobic fermentation liquid of food waste
Antonie Van Leeuwenhoek. 2024 Feb 13;117(1):35. doi: 10.1007/s10482-024-01932-6.ABSTRACTA Gram-stain-negative, oxidase-negative, rod-shaped, motile, facultatively anaerobic bacterial strain, designated as CY1220T, was isolated from an anaerobic fermentation liquid of food waste treatment plant. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain CY1220T belongs to the genus Thiopseudomonas, with the highest sequence similarity to Thiopseudomonas alkaliphila B4199T (95.91%), followed by Thiopseudomonas denitrificans X2T (95.56%). The genomic DNA G + C content of strain CY1220T was 48.6 mol%. The...
Source: Antonie van Leeuwenhoek - February 13, 2024 Category: Microbiology Authors: Miaomiao An Ruina Liang Yanjuan Lu Xiaoxu Li Guozhu Zhao Source Type: research
Thiopseudomonas acetoxidans sp. nov., an aerobic acetic and butyric acids oxidizer isolated from anaerobic fermentation liquid of food waste
Antonie Van Leeuwenhoek. 2024 Feb 13;117(1):35. doi: 10.1007/s10482-024-01932-6.ABSTRACTA Gram-stain-negative, oxidase-negative, rod-shaped, motile, facultatively anaerobic bacterial strain, designated as CY1220T, was isolated from an anaerobic fermentation liquid of food waste treatment plant. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain CY1220T belongs to the genus Thiopseudomonas, with the highest sequence similarity to Thiopseudomonas alkaliphila B4199T (95.91%), followed by Thiopseudomonas denitrificans X2T (95.56%). The genomic DNA G + C content of strain CY1220T was 48.6 mol%. The...
Source: Antonie van Leeuwenhoek - February 13, 2024 Category: Microbiology Authors: Miaomiao An Ruina Liang Yanjuan Lu Xiaoxu Li Guozhu Zhao Source Type: research
