Ciceribacter sichuanensis sp. nov., a plant growth promoting rhizobacterium isolated from root nodules of soybean in Sichuan, China
Antonie Van Leeuwenhoek. 2024 Mar 1;117(1):46. doi: 10.1007/s10482-024-01941-5.ABSTRACTThe fast-growing rhizobia-like strains S101T and S153, isolated from root nodules of soybean (Glycine max) in Sichuan, People's Republic of China, underwent characterization using a polyphasic taxonomy approach. The strains exhibited growth at 20-40 °C (optimum, 28 °C), pH 4.0-10.0 (optimum, pH 7.0) and up to 2.0% (w/v) NaCl (optimum, 0.01%) on Yeast Mannitol Agar plates. The 16S rRNA gene of strain S101T showed 98.4% sequence similarity to the closest type strain, Ciceribacter daejeonense L61T. Major cellular fatty acids in strain S10...
Source: Antonie van Leeuwenhoek - March 1, 2024 Category: Microbiology Authors: Yanqin Zhang Yuanxue Chen Petri Penttinen Xing Wang Ying Quan Licheng Wen Miao Yang Xiaoping Zhang Qiang Chen Lingzi Zhang Junjie Zhang Xiaoxia Zhang Kaiwei Xu Source Type: research
Alcohol dehydrogenase system acts as the sole pathway for methanol oxidation in Desulfofundulus kuznetsovii strain TPOSR
In this study, we describe a newly isolated strain of D. kuznetsovii, strain TPOSR, and compare its metabolism to the type strain D. kuznetsovii 17T. Both strains grow on a large variety of alcohols, such as methanol, ethanol and propane-diols, coupled to the reduction of sulphate. Strain 17T metabolizes methanol via two routes, one involving a cobalt-dependent methyl transferase and the other using a cobalt-independent alcohol dehydrogenase. However, strain TPOSR, which shares 97% average nucleotide identity with D. kuznetsovii strain 17T, lacks several genes from the methyl transferase operon found in strain 17T. The gen...
Source: Antonie van Leeuwenhoek - March 1, 2024 Category: Microbiology Authors: Lukas Friedeheim Sjef Boeren Irene S ánchez-Andrea Alfons J M Stams Diana Z Sousa Source Type: research
Solibacillus ferritrahens sp. nov., a novel siderophore-producing bacterium isolated from Wumeng Mountain National Nature Reserve in Yunnan Province
Antonie Van Leeuwenhoek. 2024 Mar 1;117(1):48. doi: 10.1007/s10482-024-01942-4.ABSTRACTA gram-stain-positive, aerobic, rod-shaped bacterial strain capable of producing siderophores, named YIM B08730T, was isolated from a soil sample collected from Wumeng Mountain National Nature Reserve, Zhaotong City, Yunnan Province. Growth occurred at 10-45 °C (optimum, 35-40 ℃), pH 7.0-9.0 (optimum, 7.0) and in the presence of 0-5 % (w/v) NaCl (optimum, 0-1 %, w/v). A comparative analysis of the 16S rRNA gene sequence (1558 bp) of strain YIM B08730T showed the highest similarity to Solibacillus isronensis JCM 13838T (96.2 %), follow...
Source: Antonie van Leeuwenhoek - March 1, 2024 Category: Microbiology Authors: Xiao-Di Liu Jiang-Yuan Zhao Le-Le Li Jian-Yu Li Pei-Wen Yang Song-Guo Liang Lu-Yao Feng Zhu-Feng Shi Zhang-Gui Ding Ming-Gang Li Shu-Kun Tang Source Type: research
Ciceribacter sichuanensis sp. nov., a plant growth promoting rhizobacterium isolated from root nodules of soybean in Sichuan, China
Antonie Van Leeuwenhoek. 2024 Mar 1;117(1):46. doi: 10.1007/s10482-024-01941-5.ABSTRACTThe fast-growing rhizobia-like strains S101T and S153, isolated from root nodules of soybean (Glycine max) in Sichuan, People's Republic of China, underwent characterization using a polyphasic taxonomy approach. The strains exhibited growth at 20-40 °C (optimum, 28 °C), pH 4.0-10.0 (optimum, pH 7.0) and up to 2.0% (w/v) NaCl (optimum, 0.01%) on Yeast Mannitol Agar plates. The 16S rRNA gene of strain S101T showed 98.4% sequence similarity to the closest type strain, Ciceribacter daejeonense L61T. Major cellular fatty acids in strain S10...
Source: Antonie van Leeuwenhoek - March 1, 2024 Category: Microbiology Authors: Yanqin Zhang Yuanxue Chen Petri Penttinen Xing Wang Ying Quan Licheng Wen Miao Yang Xiaoping Zhang Qiang Chen Lingzi Zhang Junjie Zhang Xiaoxia Zhang Kaiwei Xu Source Type: research
Alcohol dehydrogenase system acts as the sole pathway for methanol oxidation in Desulfofundulus kuznetsovii strain TPOSR
In this study, we describe a newly isolated strain of D. kuznetsovii, strain TPOSR, and compare its metabolism to the type strain D. kuznetsovii 17T. Both strains grow on a large variety of alcohols, such as methanol, ethanol and propane-diols, coupled to the reduction of sulphate. Strain 17T metabolizes methanol via two routes, one involving a cobalt-dependent methyl transferase and the other using a cobalt-independent alcohol dehydrogenase. However, strain TPOSR, which shares 97% average nucleotide identity with D. kuznetsovii strain 17T, lacks several genes from the methyl transferase operon found in strain 17T. The gen...
Source: Antonie van Leeuwenhoek - March 1, 2024 Category: Microbiology Authors: Lukas Friedeheim Sjef Boeren Irene S ánchez-Andrea Alfons J M Stams Diana Z Sousa Source Type: research
Fluctibacter corallii gen. nov., sp. nov., isolated from the coral Montipora capitata on a reef in K āne'ohe Bay, O'ahu, Hawai'i, reclassification of Aestuariibacter halophilus as Fluctibacter halophilus comb. nov., and Paraglaciecola oceanifecundans as a later heterotypic synonym of Paraglaciecola agarilytica
Antonie Van Leeuwenhoek. 2024 Mar 1;117(1):45. doi: 10.1007/s10482-024-01934-4.ABSTRACTStrain AA17T was isolated from an apparently healthy fragment of Montipora capitata coral from the reef surrounding Moku o Lo'e in Kāne'ohe Bay, O'ahu, Hawai'i, USA, and was taxonomically evaluated using a polyphasic approach. Comparison of a partial 16S rRNA gene sequence found that strain AA17T shared the greatest similarity with Aestuariibacter halophilus JC2043T (96.6%), and phylogenies based on 16S rRNA gene sequences grouped strain AA17T with members of the Aliiglaciecola, Aestuariibacter, Lacimicrobium, Marisediminitalea, Plancto...
Source: Antonie van Leeuwenhoek - February 29, 2024 Category: Microbiology Authors: Sarah A Emsley Rachel M Loughran Maximillian D Shlafstein Kaysa M Pfannmuller Yesmarie T De La Flor Charles G Lein Nicholas C Dove Marc J Koyack David K Oline Thomas E Hanson Patrick Videau Jimmy H Saw Blake Ushijima Source Type: research
Functional characterization of a catalase gene PtCat associated with sclerotia formation in Pleurotus tuber-regium
Antonie Van Leeuwenhoek. 2024 Feb 27;117(1):42. doi: 10.1007/s10482-024-01943-3.ABSTRACTPleurotus tuber-regium (Fr.) Sing. can evade oxygen by forming sclerotia under oxidative stress, consequently averting the development of hyperoxidative state, during which the expression level of catalase gene (PtCat) is significantly up-regulated. To investigate the relationship between the catalase gene and sclerotia formation, over-expression and interference strains of the PtCat gene were obtained by Agrobacterium tumefaciens-mediated transformation for phenotypic analysis. In the absence of hydrogen peroxide (H2O2) stress, a minor...
Source: Antonie van Leeuwenhoek - February 27, 2024 Category: Microbiology Authors: Xuan Zhou Li Sheng Yingjuan Li Aimin Ma Source Type: research
The surface of leaves and fruits of Peruvian cacao is home for several Hannaella yeast species, including the new species Hannaella theobromatis sp. nov
Antonie Van Leeuwenhoek. 2024 Feb 27;117(1):43. doi: 10.1007/s10482-024-01936-2.ABSTRACTAs part of a long-term study aiming to isolate and identify yeast species that inhabit the surface of leaves and fruits of native fine-aroma cacao in the department of Amazonas, Peru, we obtained multiple isolates of Hannaella species. Yeasts of the genus Hannaella are common inhabitants of the phyllosphere of natural and crop plants. On the basis of morphological, and physiological characteristics, and sequence analysis of the D1/D2 domains of the large subunit rRNA gene (LSU) and the internal transcribed spacer region (ITS), we identi...
Source: Antonie van Leeuwenhoek - February 27, 2024 Category: Microbiology Authors: Kelvin J Llanos-G ómez M Catherine Aime Jorge R D íaz-Valderrama Source Type: research
Talaromyces sedimenticola sp. nov., isolated from the Mariana Trench
Antonie Van Leeuwenhoek. 2024 Feb 28;117(1):44. doi: 10.1007/s10482-024-01945-1.ABSTRACTTwo fungal strains (K-2T and S1) were isolated from the deepest ocean sediment of the Challenger Deep located in the Mariana Trench. The internal transcribed spacer (ITS) gene sequences of the isolates K-2T and S1 differed from those of closely related species, such as Talaromyces assiutensis and T. trachyspermus. Phylogenetic analyses based on single and concatenated alignments of the genes, namely ITS, β-tubulin (benA), calmodulin (cam), and the second-largest subunit fragment of the RNA polymerase II (rpb2) showed that the isolates ...
Source: Antonie van Leeuwenhoek - February 27, 2024 Category: Microbiology Authors: Hongbo Zhou Liting Xu Wenxian Liu Kaiwen Ta Xincun Wang Jianwei Guo Wenxi Luo Zhiyuan Peng Qiaoni Huang Yuguang Wang Source Type: research
Functional characterization of a catalase gene PtCat associated with sclerotia formation in Pleurotus tuber-regium
Antonie Van Leeuwenhoek. 2024 Feb 27;117(1):42. doi: 10.1007/s10482-024-01943-3.ABSTRACTPleurotus tuber-regium (Fr.) Sing. can evade oxygen by forming sclerotia under oxidative stress, consequently averting the development of hyperoxidative state, during which the expression level of catalase gene (PtCat) is significantly up-regulated. To investigate the relationship between the catalase gene and sclerotia formation, over-expression and interference strains of the PtCat gene were obtained by Agrobacterium tumefaciens-mediated transformation for phenotypic analysis. In the absence of hydrogen peroxide (H2O2) stress, a minor...
Source: Antonie van Leeuwenhoek - February 27, 2024 Category: Microbiology Authors: Xuan Zhou Li Sheng Yingjuan Li Aimin Ma Source Type: research
The surface of leaves and fruits of Peruvian cacao is home for several Hannaella yeast species, including the new species Hannaella theobromatis sp. nov
Antonie Van Leeuwenhoek. 2024 Feb 27;117(1):43. doi: 10.1007/s10482-024-01936-2.ABSTRACTAs part of a long-term study aiming to isolate and identify yeast species that inhabit the surface of leaves and fruits of native fine-aroma cacao in the department of Amazonas, Peru, we obtained multiple isolates of Hannaella species. Yeasts of the genus Hannaella are common inhabitants of the phyllosphere of natural and crop plants. On the basis of morphological, and physiological characteristics, and sequence analysis of the D1/D2 domains of the large subunit rRNA gene (LSU) and the internal transcribed spacer region (ITS), we identi...
Source: Antonie van Leeuwenhoek - February 27, 2024 Category: Microbiology Authors: Kelvin J Llanos-G ómez M Catherine Aime Jorge R D íaz-Valderrama Source Type: research
Talaromyces sedimenticola sp. nov., isolated from the Mariana Trench
Antonie Van Leeuwenhoek. 2024 Feb 28;117(1):44. doi: 10.1007/s10482-024-01945-1.ABSTRACTTwo fungal strains (K-2T and S1) were isolated from the deepest ocean sediment of the Challenger Deep located in the Mariana Trench. The internal transcribed spacer (ITS) gene sequences of the isolates K-2T and S1 differed from those of closely related species, such as Talaromyces assiutensis and T. trachyspermus. Phylogenetic analyses based on single and concatenated alignments of the genes, namely ITS, β-tubulin (benA), calmodulin (cam), and the second-largest subunit fragment of the RNA polymerase II (rpb2) showed that the isolates ...
Source: Antonie van Leeuwenhoek - February 27, 2024 Category: Microbiology Authors: Hongbo Zhou Liting Xu Wenxian Liu Kaiwen Ta Xincun Wang Jianwei Guo Wenxi Luo Zhiyuan Peng Qiaoni Huang Yuguang Wang Source Type: research
Functional characterization of a catalase gene PtCat associated with sclerotia formation in Pleurotus tuber-regium
Antonie Van Leeuwenhoek. 2024 Feb 27;117(1):42. doi: 10.1007/s10482-024-01943-3.ABSTRACTPleurotus tuber-regium (Fr.) Sing. can evade oxygen by forming sclerotia under oxidative stress, consequently averting the development of hyperoxidative state, during which the expression level of catalase gene (PtCat) is significantly up-regulated. To investigate the relationship between the catalase gene and sclerotia formation, over-expression and interference strains of the PtCat gene were obtained by Agrobacterium tumefaciens-mediated transformation for phenotypic analysis. In the absence of hydrogen peroxide (H2O2) stress, a minor...
Source: Antonie van Leeuwenhoek - February 27, 2024 Category: Microbiology Authors: Xuan Zhou Li Sheng Yingjuan Li Aimin Ma Source Type: research
Pseudomonas environmental strain produces a DegQ-derived and PDZ domain containing peptide with protease activity
This report provides the isolation and identification of a highly proteolytic fragment derived from DegQ produced by a Pseudomonas fluorescens environmental strain isolated from a frog carcass. Zymograms demonstrate that a 10 kDa protein mainly generates the total proteolytic activity of this strain, which is enhanced by the detergent SDS. Mass spectroscopy analysis revealed that the protein derived a couple of peptides, the ones showing the highest coverage belonging to DegQ. Interestingly, this small protein fragment contains a PDZ domain but no obvious residues indicating that it is a protease. Protein model analysis sh...
Source: Antonie van Leeuwenhoek - February 24, 2024 Category: Microbiology Authors: Francisco Vargas-Gasca Bernardo Franco Naur ú Idalia Vargas-Maya Marcos Vicente-G ómez Vianey Olmedo-Monfil Source Type: research
Pseudomonas environmental strain produces a DegQ-derived and PDZ domain containing peptide with protease activity
This report provides the isolation and identification of a highly proteolytic fragment derived from DegQ produced by a Pseudomonas fluorescens environmental strain isolated from a frog carcass. Zymograms demonstrate that a 10 kDa protein mainly generates the total proteolytic activity of this strain, which is enhanced by the detergent SDS. Mass spectroscopy analysis revealed that the protein derived a couple of peptides, the ones showing the highest coverage belonging to DegQ. Interestingly, this small protein fragment contains a PDZ domain but no obvious residues indicating that it is a protease. Protein model analysis sh...
Source: Antonie van Leeuwenhoek - February 24, 2024 Category: Microbiology Authors: Francisco Vargas-Gasca Bernardo Franco Naur ú Idalia Vargas-Maya Marcos Vicente-G ómez Vianey Olmedo-Monfil Source Type: research
