Emticicia fluvialis sp. nov., a potential hormone-degrading bacterium isolated from Nakdong River, Republic of Korea
Antonie Van Leeuwenhoek. 2023 Sep 29. doi: 10.1007/s10482-023-01889-y. Online ahead of print.ABSTRACTA novel species of the genus Emticicia, designated BHSR1T, was isolated from a water sample that was collected from the Nakdong River, Republic of Korea, and its taxonomic affiliation was studied using a polyphasic approach. This bacterium was Gram-stain-negative, non-motile, aerobic, curved, rod-shaped, and oxidase- and catalase-negative. The bacterium grew optimally at 37 °C, pH 7.5 and 0% (w/v) NaCl. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain BHSR1T should be affiliated with the genus E...
Source: Antonie van Leeuwenhoek - September 29, 2023 Category: Microbiology Authors: Hyun-Sun Baek Yong Guan Min-Ju Kim Yue Jiang Mi-Kyung Lee Ki-Hyun Kim Jaeyoon Lee Yuna Shin Yoon-Ho Kang Zhun Li Source Type: research

Fluviispira vulneris sp. nov., isolated from human wound secretions
In this study, we reported a new environmental pathogen. Isolate GX5518T was a novel Gram-negative, aerobic, non-motile, pleomorphic and red-pigmented bacterium, was isolated from human wound secretions (GuangXi, People's Republic of China). Growth occurred at pH 6.0-8.0 (optimum, pH 7.0) and 10-37 °C (optimum, 28-32 °C) with 0-1.5% (w/v) NaCl in R2A agar. Comparative analysis of the 16S rRNA gene sequences revealed that isolate GX5518T was closely related to Fluviispira sanaruensis JCM 31447T (99.73%) and Fluviispira multicolorata 33A1-SZDPT (98.49%). However, the estimated ANI values of the isolate GX5518T compared to ...
Source: Antonie van Leeuwenhoek - September 29, 2023 Category: Microbiology Authors: Peijuan Tang Na Peng Pengwen Ouyang Sheng Long Zhenhua Wei Xingchun Chen Pinghua Qu Liangyi Xie Source Type: research

Emticicia fluvialis sp. nov., a potential hormone-degrading bacterium isolated from Nakdong River, Republic of Korea
Antonie Van Leeuwenhoek. 2023 Sep 29. doi: 10.1007/s10482-023-01889-y. Online ahead of print.ABSTRACTA novel species of the genus Emticicia, designated BHSR1T, was isolated from a water sample that was collected from the Nakdong River, Republic of Korea, and its taxonomic affiliation was studied using a polyphasic approach. This bacterium was Gram-stain-negative, non-motile, aerobic, curved, rod-shaped, and oxidase- and catalase-negative. The bacterium grew optimally at 37 °C, pH 7.5 and 0% (w/v) NaCl. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain BHSR1T should be affiliated with the genus E...
Source: Antonie van Leeuwenhoek - September 29, 2023 Category: Microbiology Authors: Hyun-Sun Baek Yong Guan Min-Ju Kim Yue Jiang Mi-Kyung Lee Ki-Hyun Kim Jaeyoon Lee Yuna Shin Yoon-Ho Kang Zhun Li Source Type: research

Fluviispira vulneris sp. nov., isolated from human wound secretions
In this study, we reported a new environmental pathogen. Isolate GX5518T was a novel Gram-negative, aerobic, non-motile, pleomorphic and red-pigmented bacterium, was isolated from human wound secretions (GuangXi, People's Republic of China). Growth occurred at pH 6.0-8.0 (optimum, pH 7.0) and 10-37 °C (optimum, 28-32 °C) with 0-1.5% (w/v) NaCl in R2A agar. Comparative analysis of the 16S rRNA gene sequences revealed that isolate GX5518T was closely related to Fluviispira sanaruensis JCM 31447T (99.73%) and Fluviispira multicolorata 33A1-SZDPT (98.49%). However, the estimated ANI values of the isolate GX5518T compared to ...
Source: Antonie van Leeuwenhoek - September 29, 2023 Category: Microbiology Authors: Peijuan Tang Na Peng Pengwen Ouyang Sheng Long Zhenhua Wei Xingchun Chen Pinghua Qu Liangyi Xie Source Type: research

Emticicia fluvialis sp. nov., a potential hormone-degrading bacterium isolated from Nakdong River, Republic of Korea
Antonie Van Leeuwenhoek. 2023 Sep 29. doi: 10.1007/s10482-023-01889-y. Online ahead of print.ABSTRACTA novel species of the genus Emticicia, designated BHSR1T, was isolated from a water sample that was collected from the Nakdong River, Republic of Korea, and its taxonomic affiliation was studied using a polyphasic approach. This bacterium was Gram-stain-negative, non-motile, aerobic, curved, rod-shaped, and oxidase- and catalase-negative. The bacterium grew optimally at 37 °C, pH 7.5 and 0% (w/v) NaCl. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain BHSR1T should be affiliated with the genus E...
Source: Antonie van Leeuwenhoek - September 29, 2023 Category: Microbiology Authors: Hyun-Sun Baek Yong Guan Min-Ju Kim Yue Jiang Mi-Kyung Lee Ki-Hyun Kim Jaeyoon Lee Yuna Shin Yoon-Ho Kang Zhun Li Source Type: research

Fluviispira vulneris sp. nov., isolated from human wound secretions
In this study, we reported a new environmental pathogen. Isolate GX5518T was a novel Gram-negative, aerobic, non-motile, pleomorphic and red-pigmented bacterium, was isolated from human wound secretions (GuangXi, People's Republic of China). Growth occurred at pH 6.0-8.0 (optimum, pH 7.0) and 10-37 °C (optimum, 28-32 °C) with 0-1.5% (w/v) NaCl in R2A agar. Comparative analysis of the 16S rRNA gene sequences revealed that isolate GX5518T was closely related to Fluviispira sanaruensis JCM 31447T (99.73%) and Fluviispira multicolorata 33A1-SZDPT (98.49%). However, the estimated ANI values of the isolate GX5518T compared to ...
Source: Antonie van Leeuwenhoek - September 29, 2023 Category: Microbiology Authors: Peijuan Tang Na Peng Pengwen Ouyang Sheng Long Zhenhua Wei Xingchun Chen Pinghua Qu Liangyi Xie Source Type: research

Emticicia fluvialis sp. nov., a potential hormone-degrading bacterium isolated from Nakdong River, Republic of Korea
Antonie Van Leeuwenhoek. 2023 Sep 29. doi: 10.1007/s10482-023-01889-y. Online ahead of print.ABSTRACTA novel species of the genus Emticicia, designated BHSR1T, was isolated from a water sample that was collected from the Nakdong River, Republic of Korea, and its taxonomic affiliation was studied using a polyphasic approach. This bacterium was Gram-stain-negative, non-motile, aerobic, curved, rod-shaped, and oxidase- and catalase-negative. The bacterium grew optimally at 37 °C, pH 7.5 and 0% (w/v) NaCl. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain BHSR1T should be affiliated with the genus E...
Source: Antonie van Leeuwenhoek - September 29, 2023 Category: Microbiology Authors: Hyun-Sun Baek Yong Guan Min-Ju Kim Yue Jiang Mi-Kyung Lee Ki-Hyun Kim Jaeyoon Lee Yuna Shin Yoon-Ho Kang Zhun Li Source Type: research

Fluviispira vulneris sp. nov., isolated from human wound secretions
In this study, we reported a new environmental pathogen. Isolate GX5518T was a novel Gram-negative, aerobic, non-motile, pleomorphic and red-pigmented bacterium, was isolated from human wound secretions (GuangXi, People's Republic of China). Growth occurred at pH 6.0-8.0 (optimum, pH 7.0) and 10-37 °C (optimum, 28-32 °C) with 0-1.5% (w/v) NaCl in R2A agar. Comparative analysis of the 16S rRNA gene sequences revealed that isolate GX5518T was closely related to Fluviispira sanaruensis JCM 31447T (99.73%) and Fluviispira multicolorata 33A1-SZDPT (98.49%). However, the estimated ANI values of the isolate GX5518T compared to ...
Source: Antonie van Leeuwenhoek - September 29, 2023 Category: Microbiology Authors: Peijuan Tang Na Peng Pengwen Ouyang Sheng Long Zhenhua Wei Xingchun Chen Pinghua Qu Liangyi Xie Source Type: research

Emticicia fluvialis sp. nov., a potential hormone-degrading bacterium isolated from Nakdong River, Republic of Korea
Antonie Van Leeuwenhoek. 2023 Sep 29. doi: 10.1007/s10482-023-01889-y. Online ahead of print.ABSTRACTA novel species of the genus Emticicia, designated BHSR1T, was isolated from a water sample that was collected from the Nakdong River, Republic of Korea, and its taxonomic affiliation was studied using a polyphasic approach. This bacterium was Gram-stain-negative, non-motile, aerobic, curved, rod-shaped, and oxidase- and catalase-negative. The bacterium grew optimally at 37 °C, pH 7.5 and 0% (w/v) NaCl. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain BHSR1T should be affiliated with the genus E...
Source: Antonie van Leeuwenhoek - September 29, 2023 Category: Microbiology Authors: Hyun-Sun Baek Yong Guan Min-Ju Kim Yue Jiang Mi-Kyung Lee Ki-Hyun Kim Jaeyoon Lee Yuna Shin Yoon-Ho Kang Zhun Li Source Type: research

Fluviispira vulneris sp. nov., isolated from human wound secretions
In this study, we reported a new environmental pathogen. Isolate GX5518T was a novel Gram-negative, aerobic, non-motile, pleomorphic and red-pigmented bacterium, was isolated from human wound secretions (GuangXi, People's Republic of China). Growth occurred at pH 6.0-8.0 (optimum, pH 7.0) and 10-37 °C (optimum, 28-32 °C) with 0-1.5% (w/v) NaCl in R2A agar. Comparative analysis of the 16S rRNA gene sequences revealed that isolate GX5518T was closely related to Fluviispira sanaruensis JCM 31447T (99.73%) and Fluviispira multicolorata 33A1-SZDPT (98.49%). However, the estimated ANI values of the isolate GX5518T compared to ...
Source: Antonie van Leeuwenhoek - September 29, 2023 Category: Microbiology Authors: Peijuan Tang Na Peng Pengwen Ouyang Sheng Long Zhenhua Wei Xingchun Chen Pinghua Qu Liangyi Xie Source Type: research

Novel Metschnikowia yeasts from the gut of green lacewing in Japan
Antonie Van Leeuwenhoek. 2023 Sep 27. doi: 10.1007/s10482-023-01887-0. Online ahead of print.ABSTRACTFamily Chrysopidae is known to harbor specific gut yeasts. However, no studies have been conducted outside of a limited number of these green lacewing species, and the diversity of yeasts in the family as a whole is not known. Therefore, we collected 58 Chrysopidae adults (9 species, 6 genera, 2 subfamilies) in Japan and isolated yeasts from all individuals. The results showed for the first time that not only subfamily Chrysopinae but also subfamily Apochrysinae have gut yeasts. We obtained 58 yeast isolates (one from each ...
Source: Antonie van Leeuwenhoek - September 27, 2023 Category: Microbiology Authors: Yuma Yoshihashi Yousuke Degawa Source Type: research

Novel Metschnikowia yeasts from the gut of green lacewing in Japan
Antonie Van Leeuwenhoek. 2023 Sep 27. doi: 10.1007/s10482-023-01887-0. Online ahead of print.ABSTRACTFamily Chrysopidae is known to harbor specific gut yeasts. However, no studies have been conducted outside of a limited number of these green lacewing species, and the diversity of yeasts in the family as a whole is not known. Therefore, we collected 58 Chrysopidae adults (9 species, 6 genera, 2 subfamilies) in Japan and isolated yeasts from all individuals. The results showed for the first time that not only subfamily Chrysopinae but also subfamily Apochrysinae have gut yeasts. We obtained 58 yeast isolates (one from each ...
Source: Antonie van Leeuwenhoek - September 27, 2023 Category: Microbiology Authors: Yuma Yoshihashi Yousuke Degawa Source Type: research

Novel Metschnikowia yeasts from the gut of green lacewing in Japan
Antonie Van Leeuwenhoek. 2023 Sep 27. doi: 10.1007/s10482-023-01887-0. Online ahead of print.ABSTRACTFamily Chrysopidae is known to harbor specific gut yeasts. However, no studies have been conducted outside of a limited number of these green lacewing species, and the diversity of yeasts in the family as a whole is not known. Therefore, we collected 58 Chrysopidae adults (9 species, 6 genera, 2 subfamilies) in Japan and isolated yeasts from all individuals. The results showed for the first time that not only subfamily Chrysopinae but also subfamily Apochrysinae have gut yeasts. We obtained 58 yeast isolates (one from each ...
Source: Antonie van Leeuwenhoek - September 27, 2023 Category: Microbiology Authors: Yuma Yoshihashi Yousuke Degawa Source Type: research

Expression of toxic genes in Methylorubrum extorquens with a tightly repressed, cumate-inducible promoter
Antonie Van Leeuwenhoek. 2023 Sep 26. doi: 10.1007/s10482-023-01880-7. Online ahead of print.ABSTRACTMethylorubrum extorquens is an important model methylotroph and has enormous potential for the development of C1-based microbial cell factories. During strain construction, regulated promoters with a low background expression level are important genetic tools for expression of potentially toxic genes. Here we present an accordingly optimised promoter, which can be used for that purpose. During construction and testing of terpene production strains harbouring a recombinant mevalonate pathway, strong growth defects were obser...
Source: Antonie van Leeuwenhoek - September 26, 2023 Category: Microbiology Authors: Laura P öschel Elisabeth Gehr Paulina Jordan Frank Sonntag Markus Buchhaupt Source Type: research

Streptomyces cynarae sp. nov., a novel actinomycete isolated from the leaves of Cynara scolymus L
Antonie Van Leeuwenhoek. 2023 Sep 25. doi: 10.1007/s10482-023-01885-2. Online ahead of print.ABSTRACTStrain HUAS 13-4T, a novel endophytic actinobacterium, was isolated from the leaves of Cynara scolymus L. collected from Changde City in China and characterized using a polyphasic approach. Based on 16S rRNA gene sequence analysis, strain HUAS 13-4T shared the highest sequence similarities to Streptomyces leeuwenhoekii C34T (98.90%), Streptomyces harenosi PRKS01-65T (98.83%) and Streptomyces glomeratus LMG 19903T (98.76%). Phylogenetic analysis of 16S rRNA gene sequence indicated that strain HUAS 13-4T was clustered togethe...
Source: Antonie van Leeuwenhoek - September 25, 2023 Category: Microbiology Authors: Aihua Deng Yijia Luo Can Wu Dan Chen Ping Mo Baiyuan Li Source Type: research