Comparative genomics of an extensively drug resistant strain Klebsiella pneumoniae IITR008 with international high-risk clonal lineage ST147 isolated from river water
Antonie Van Leeuwenhoek. 2024 Mar 15;117(1):57. doi: 10.1007/s10482-024-01955-z.ABSTRACTCarbapenem resistant Klebsiella pneumoniae causing severe infection resulting in morbidity and mortality have become a global health concern. K. pneumoniae with sequence type ST147 is an international high-risk clonal lineage, genomic studies have been done on K. pneumoniae ST147 isolated from clinical origin but genomic data for environmental K. pneumoniae ST147 is very scarce. Herein, K. pneumoniae IITR008, an extensively drug resistant and potentially hypervirulent bacterium, was isolated from Triveni Sangam, the confluence of three ...
Source: Antonie van Leeuwenhoek - March 16, 2024 Category: Microbiology Authors: Deeksha Singh Shilpee Pal Srikrishna Subramanian Natesan Manickam Source Type: research
Comparative genomics of an extensively drug resistant strain Klebsiella pneumoniae IITR008 with international high-risk clonal lineage ST147 isolated from river water
Antonie Van Leeuwenhoek. 2024 Mar 15;117(1):57. doi: 10.1007/s10482-024-01955-z.ABSTRACTCarbapenem resistant Klebsiella pneumoniae causing severe infection resulting in morbidity and mortality have become a global health concern. K. pneumoniae with sequence type ST147 is an international high-risk clonal lineage, genomic studies have been done on K. pneumoniae ST147 isolated from clinical origin but genomic data for environmental K. pneumoniae ST147 is very scarce. Herein, K. pneumoniae IITR008, an extensively drug resistant and potentially hypervirulent bacterium, was isolated from Triveni Sangam, the confluence of three ...
Source: Antonie van Leeuwenhoek - March 16, 2024 Category: Microbiology Authors: Deeksha Singh Shilpee Pal Srikrishna Subramanian Natesan Manickam Source Type: research
Antimicrobial peptides designed by computational analysis of proteomes
This study's most active antimicrobial peptides damage the bacterial cell membrane, including grooves, dents, membrane wrinkling, cell destruction, and leakage of cytoplasmic material. The results confirm that the proposed approach, which uses bioinformatic tools and rational modifications, is highly efficient and allows the discovery, with high accuracy, of potent AMPs encrypted in proteins.PMID:38488950 | DOI:10.1007/s10482-024-01946-0 (Source: Antonie van Leeuwenhoek)
Source: Antonie van Leeuwenhoek - March 15, 2024 Category: Microbiology Authors: Dahiana Monsalve Andrea Mesa Laura M Mira Carlos Mera Sergio Orduz John W Branch-Bedoya Source Type: research
Maribacter halichondriae sp. nov., isolated from the marine sponge Halichondria panicea, displays features of a sponge-associated life style
Antonie Van Leeuwenhoek. 2024 Mar 15;117(1):56. doi: 10.1007/s10482-024-01950-4.ABSTRACTA new member of the family Flavobacteriaceae (termed Hal144T) was isolated from the marine breadcrumb sponge Halichondria panicea. Sponge material was collected in 2018 at Schilksee which is located in the Kiel Fjord (Baltic Sea, Germany). Phylogenetic analysis of the full-length Hal144T 16S rRNA gene sequence revealed similarities from 94.3 to 96.6% to the nearest type strains of the genus Maribacter. The phylogenetic tree of the 16S rRNA gene sequences depicted a cluster of strain Hal144T with its closest relatives Maribacter aestuari...
Source: Antonie van Leeuwenhoek - March 15, 2024 Category: Microbiology Authors: Leon X Steiner Jutta Wiese Tanja Rahn Erik Borchert Beate M Slaby Ute Hentschel Source Type: research
Expression of Staphylococcus aureus translation elongation factor P is regulated by a stress-inducible promotor
Antonie Van Leeuwenhoek. 2024 Mar 15;117(1):54. doi: 10.1007/s10482-024-01954-0.ABSTRACTTranslation elongation factor P, expressed by the efp gene, is a conserved protein closely related to bacterial virulence and environmental stress regulation responses, however, little is known about the efp gene expression regulations. Here, the strain of Staphylococcus aureus subsp. aureus NCTC 8325 was taken as the research object and cultured under different conditions, including different culture temperatures, pH, and antibiotics, to study the expression of the efp gene in S. aureus by qRT-PCR, the results showed that the expressio...
Source: Antonie van Leeuwenhoek - March 15, 2024 Category: Microbiology Authors: Xingxing Zheng Xiuhui Sun Weiwei Xiang Haiyan Ni Long Zou Zhong-Er Long Source Type: research
Antimicrobial peptides designed by computational analysis of proteomes
This study's most active antimicrobial peptides damage the bacterial cell membrane, including grooves, dents, membrane wrinkling, cell destruction, and leakage of cytoplasmic material. The results confirm that the proposed approach, which uses bioinformatic tools and rational modifications, is highly efficient and allows the discovery, with high accuracy, of potent AMPs encrypted in proteins.PMID:38488950 | DOI:10.1007/s10482-024-01946-0 (Source: Antonie van Leeuwenhoek)
Source: Antonie van Leeuwenhoek - March 15, 2024 Category: Microbiology Authors: Dahiana Monsalve Andrea Mesa Laura M Mira Carlos Mera Sergio Orduz John W Branch-Bedoya Source Type: research
Maribacter halichondriae sp. nov., isolated from the marine sponge Halichondria panicea, displays features of a sponge-associated life style
Antonie Van Leeuwenhoek. 2024 Mar 15;117(1):56. doi: 10.1007/s10482-024-01950-4.ABSTRACTA new member of the family Flavobacteriaceae (termed Hal144T) was isolated from the marine breadcrumb sponge Halichondria panicea. Sponge material was collected in 2018 at Schilksee which is located in the Kiel Fjord (Baltic Sea, Germany). Phylogenetic analysis of the full-length Hal144T 16S rRNA gene sequence revealed similarities from 94.3 to 96.6% to the nearest type strains of the genus Maribacter. The phylogenetic tree of the 16S rRNA gene sequences depicted a cluster of strain Hal144T with its closest relatives Maribacter aestuari...
Source: Antonie van Leeuwenhoek - March 15, 2024 Category: Microbiology Authors: Leon X Steiner Jutta Wiese Tanja Rahn Erik Borchert Beate M Slaby Ute Hentschel Source Type: research
Expression of Staphylococcus aureus translation elongation factor P is regulated by a stress-inducible promotor
Antonie Van Leeuwenhoek. 2024 Mar 15;117(1):54. doi: 10.1007/s10482-024-01954-0.ABSTRACTTranslation elongation factor P, expressed by the efp gene, is a conserved protein closely related to bacterial virulence and environmental stress regulation responses, however, little is known about the efp gene expression regulations. Here, the strain of Staphylococcus aureus subsp. aureus NCTC 8325 was taken as the research object and cultured under different conditions, including different culture temperatures, pH, and antibiotics, to study the expression of the efp gene in S. aureus by qRT-PCR, the results showed that the expressio...
Source: Antonie van Leeuwenhoek - March 15, 2024 Category: Microbiology Authors: Xingxing Zheng Xiuhui Sun Weiwei Xiang Haiyan Ni Long Zou Zhong-Er Long Source Type: research
200 years of taxonomic confusion: Sporendonema and allies
Antonie Van Leeuwenhoek. 2024 Mar 14;117(1):53. doi: 10.1007/s10482-024-01935-3.ABSTRACTThe genus Sporendonema (Gymnoascaceae, Onygenales) was introduced in 1827 with the type species S. casei for a red mould on cheese. Cheese is a consistent niche for this species. Sphaerosporium equinum is another species classified in Gymnoascaceae and has also been reported from cheese. Recently, other habitats have been reported for both Sporendonema casei and Sphaerosporium equinum. The present study aimed to investigate the taxonomy of Sporendonema and Sphaerosporium, as well as a close neighbour, Arachniotus. Two strains of Hormisc...
Source: Antonie van Leeuwenhoek - March 14, 2024 Category: Microbiology Authors: Hazal Kandemir Cony Decock Margarita Hern ández-Restrepo Roman Labuda Jos Houbraken Macit Ilkit G Sybren de Hoog Source Type: research
Veillonella faecalis sp. nov., a propionic acid-producing bacterium isolated from the faeces of an infant
Antonie Van Leeuwenhoek. 2024 Mar 12;117(1):50. doi: 10.1007/s10482-024-01951-3.ABSTRACTA strictly anaerobic, Gram-stain-negative, catalase-negative, cocci-shaped, and propionate-producing bacterial strain, named Ds1651T was isolated from the fecal sample collected from a South Korean infant. Through a comparison of 16S rRNA gene sequences, it was revealed that Ds1651T had the highest phylogenetic affinity with Veillonella nakazawae KCTC 25297 T (99.86%), followed by Veillonella infantium KCTC 25370 T (99.80%), and Veillonella dispar KCTC 25309 T (99.73%) in the family Veillonellaceae. Average nucleotide identity values be...
Source: Antonie van Leeuwenhoek - March 13, 2024 Category: Microbiology Authors: Haneol Yang Hana Jo Seung Hyun Kim Chan-Seok Yun Seung-Hwan Park Doo-Sang Park Source Type: research
Genome-based classification of genera Halosegnis and Salella, and description of four novel halophilic archaea isolated from a tidal flat
Antonie Van Leeuwenhoek. 2024 Mar 12;117(1):51. doi: 10.1007/s10482-024-01952-2.ABSTRACTThe current species of Halosegnis and Salella within the class Halobacteria are closely related based on phylogenetic, phylogenomic, and comparative genomic analyses. The Halosegnis species showed 99.8-100.0% 16S rRNA and 96.6-99.6% rpoB' gene similarities to the Salella species, respectively. Phylogenetic and phylogenomic analyses showed that Salella cibi CBA1133T, the sole species of Salella, formed a single tight cluster with Halosegnis longus F12-1T, then with Halosegnis rubeus F17-44T. The average nucleotide identity (ANI), digital...
Source: Antonie van Leeuwenhoek - March 13, 2024 Category: Microbiology Authors: Yao Hu Xue Ma Shun Tan Xin-Xin Li Mu Cheng Jing Hou Heng-Lin Cui Source Type: research
Description of Fuscovulum ytuae sp. nov, a facultative autotroph isolated from the intertidalite of Yangma island, China
In this study, we reported a Gram-stain-negative, ovoid to rod-shaped, atrichous, and facultative anaerobe bacteria strain named YMD61T, which was isolated from the intertidal sediment of Yangma island, China. Growth of strain YMD61T occurred at 10.0-45.0 °C (optimum, 30.0 °C), pH 7.0-10.0 (optimum, 8.0) and with 0-3.0% (w/v) NaCl (optimum, 2.0%). Phylogenetic tree analysis based on 16 S rRNA gene or genomic sequence indicated that strain YMD61T belonged to the genus Fuscovulum and was closely related to Fuscovulum blasticum ATCC 33,485T (96.6% sequence similarity). Genomic analysis indicated that strain YMD61T contains ...
Source: Antonie van Leeuwenhoek - March 13, 2024 Category: Microbiology Authors: Bin-Zhe Zhang Xiu-Dan Xu Dan-Dan Zhou Xue-Peng Li Xu-Dong Jiao Jian Zhang Source Type: research
Veillonella faecalis sp. nov., a propionic acid-producing bacterium isolated from the faeces of an infant
Antonie Van Leeuwenhoek. 2024 Mar 12;117(1):50. doi: 10.1007/s10482-024-01951-3.ABSTRACTA strictly anaerobic, Gram-stain-negative, catalase-negative, cocci-shaped, and propionate-producing bacterial strain, named Ds1651T was isolated from the fecal sample collected from a South Korean infant. Through a comparison of 16S rRNA gene sequences, it was revealed that Ds1651T had the highest phylogenetic affinity with Veillonella nakazawae KCTC 25297 T (99.86%), followed by Veillonella infantium KCTC 25370 T (99.80%), and Veillonella dispar KCTC 25309 T (99.73%) in the family Veillonellaceae. Average nucleotide identity values be...
Source: Antonie van Leeuwenhoek - March 13, 2024 Category: Microbiology Authors: Haneol Yang Hana Jo Seung Hyun Kim Chan-Seok Yun Seung-Hwan Park Doo-Sang Park Source Type: research
Genome-based classification of genera Halosegnis and Salella, and description of four novel halophilic archaea isolated from a tidal flat
Antonie Van Leeuwenhoek. 2024 Mar 12;117(1):51. doi: 10.1007/s10482-024-01952-2.ABSTRACTThe current species of Halosegnis and Salella within the class Halobacteria are closely related based on phylogenetic, phylogenomic, and comparative genomic analyses. The Halosegnis species showed 99.8-100.0% 16S rRNA and 96.6-99.6% rpoB' gene similarities to the Salella species, respectively. Phylogenetic and phylogenomic analyses showed that Salella cibi CBA1133T, the sole species of Salella, formed a single tight cluster with Halosegnis longus F12-1T, then with Halosegnis rubeus F17-44T. The average nucleotide identity (ANI), digital...
Source: Antonie van Leeuwenhoek - March 13, 2024 Category: Microbiology Authors: Yao Hu Xue Ma Shun Tan Xin-Xin Li Mu Cheng Jing Hou Heng-Lin Cui Source Type: research
Description of Fuscovulum ytuae sp. nov, a facultative autotroph isolated from the intertidalite of Yangma island, China
In this study, we reported a Gram-stain-negative, ovoid to rod-shaped, atrichous, and facultative anaerobe bacteria strain named YMD61T, which was isolated from the intertidal sediment of Yangma island, China. Growth of strain YMD61T occurred at 10.0-45.0 °C (optimum, 30.0 °C), pH 7.0-10.0 (optimum, 8.0) and with 0-3.0% (w/v) NaCl (optimum, 2.0%). Phylogenetic tree analysis based on 16 S rRNA gene or genomic sequence indicated that strain YMD61T belonged to the genus Fuscovulum and was closely related to Fuscovulum blasticum ATCC 33,485T (96.6% sequence similarity). Genomic analysis indicated that strain YMD61T contains ...
Source: Antonie van Leeuwenhoek - March 13, 2024 Category: Microbiology Authors: Bin-Zhe Zhang Xiu-Dan Xu Dan-Dan Zhou Xue-Peng Li Xu-Dong Jiao Jian Zhang Source Type: research
