Curtobacterium salicis sp. nov., isolated from willow tree stems in Washington state
Antonie Van Leeuwenhoek. 2024 Mar 29;117(1):62. doi: 10.1007/s10482-024-01956-y.ABSTRACTCurtobacterium sp. strain WW7 is a Gram-positive, non-motile, orange rod-shaped bacterium isolated from branches of wild willow (Salix sitchensis) trees. The WW7T strain has optimum growth in the temperature range between 25 and 30 °C, a pH range of 6-7.7, and tolerates up to 5.5% (w/v) of NaCl. The genome sequencing of strain WW7T revealed a genome size of approximately 3.8 Mbp and a G + C content of 71.3 mol%. The phylogenomic analyses support the WW7T affiliation to a novel Curtobacterium lineage, with Curtobacterium herbarum being ...
Source: Antonie van Leeuwenhoek - March 29, 2024 Category: Microbiology Authors: John Freeman Andrea Firrincieli Douglas Baker Sharon Doty Source Type: research
Curtobacterium salicis sp. nov., isolated from willow tree stems in Washington state
Antonie Van Leeuwenhoek. 2024 Mar 29;117(1):62. doi: 10.1007/s10482-024-01956-y.ABSTRACTCurtobacterium sp. strain WW7 is a Gram-positive, non-motile, orange rod-shaped bacterium isolated from branches of wild willow (Salix sitchensis) trees. The WW7T strain has optimum growth in the temperature range between 25 and 30 °C, a pH range of 6-7.7, and tolerates up to 5.5% (w/v) of NaCl. The genome sequencing of strain WW7T revealed a genome size of approximately 3.8 Mbp and a G + C content of 71.3 mol%. The phylogenomic analyses support the WW7T affiliation to a novel Curtobacterium lineage, with Curtobacterium herbarum being ...
Source: Antonie van Leeuwenhoek - March 29, 2024 Category: Microbiology Authors: John Freeman Andrea Firrincieli Douglas Baker Sharon Doty Source Type: research
Curtobacterium salicis sp. nov., isolated from willow tree stems in Washington state
Antonie Van Leeuwenhoek. 2024 Mar 29;117(1):62. doi: 10.1007/s10482-024-01956-y.ABSTRACTCurtobacterium sp. strain WW7 is a Gram-positive, non-motile, orange rod-shaped bacterium isolated from branches of wild willow (Salix sitchensis) trees. The WW7T strain has optimum growth in the temperature range between 25 and 30 °C, a pH range of 6-7.7, and tolerates up to 5.5% (w/v) of NaCl. The genome sequencing of strain WW7T revealed a genome size of approximately 3.8 Mbp and a G + C content of 71.3 mol%. The phylogenomic analyses support the WW7T affiliation to a novel Curtobacterium lineage, with Curtobacterium herbarum being ...
Source: Antonie van Leeuwenhoek - March 29, 2024 Category: Microbiology Authors: John Freeman Andrea Firrincieli Douglas Baker Sharon Doty Source Type: research
Lyophilization for bacteria preservation: a promising approach for Yersinia pestis strains from an unique collection in Brazil (Fiocruz-CYP)
Antonie Van Leeuwenhoek. 2024 Mar 23;117(1):61. doi: 10.1007/s10482-024-01949-x.ABSTRACTYersinia pestis, the causative agent of plague, is a highly virulent bacterium that poses a significant threat to human health. Preserving this bacterium in a viable state is crucial for research and diagnostic purposes. This paper presents and evaluates a simple lyophilization protocol for the long-term storage of Y. pestis strains from Fiocruz-CYP, aiming to explore its impact on viability and long-term stability, while replacing the currently used methodologies. The lyophilization tests were conducted using the non-virulent Y. pestis...
Source: Antonie van Leeuwenhoek - March 23, 2024 Category: Microbiology Authors: Igor Vasconcelos Rocha Matheus Filgueira Bezerra Marise Sobreira Nilma Cintra Leal Alzira Maria Paiva de Almeida Source Type: research
Lyophilization for bacteria preservation: a promising approach for Yersinia pestis strains from an unique collection in Brazil (Fiocruz-CYP)
Antonie Van Leeuwenhoek. 2024 Mar 23;117(1):61. doi: 10.1007/s10482-024-01949-x.ABSTRACTYersinia pestis, the causative agent of plague, is a highly virulent bacterium that poses a significant threat to human health. Preserving this bacterium in a viable state is crucial for research and diagnostic purposes. This paper presents and evaluates a simple lyophilization protocol for the long-term storage of Y. pestis strains from Fiocruz-CYP, aiming to explore its impact on viability and long-term stability, while replacing the currently used methodologies. The lyophilization tests were conducted using the non-virulent Y. pestis...
Source: Antonie van Leeuwenhoek - March 23, 2024 Category: Microbiology Authors: Igor Vasconcelos Rocha Matheus Filgueira Bezerra Marise Sobreira Nilma Cintra Leal Alzira Maria Paiva de Almeida Source Type: research
Lyophilization for bacteria preservation: a promising approach for Yersinia pestis strains from an unique collection in Brazil (Fiocruz-CYP)
Antonie Van Leeuwenhoek. 2024 Mar 23;117(1):61. doi: 10.1007/s10482-024-01949-x.ABSTRACTYersinia pestis, the causative agent of plague, is a highly virulent bacterium that poses a significant threat to human health. Preserving this bacterium in a viable state is crucial for research and diagnostic purposes. This paper presents and evaluates a simple lyophilization protocol for the long-term storage of Y. pestis strains from Fiocruz-CYP, aiming to explore its impact on viability and long-term stability, while replacing the currently used methodologies. The lyophilization tests were conducted using the non-virulent Y. pestis...
Source: Antonie van Leeuwenhoek - March 23, 2024 Category: Microbiology Authors: Igor Vasconcelos Rocha Matheus Filgueira Bezerra Marise Sobreira Nilma Cintra Leal Alzira Maria Paiva de Almeida Source Type: research
Lyophilization for bacteria preservation: a promising approach for Yersinia pestis strains from an unique collection in Brazil (Fiocruz-CYP)
Antonie Van Leeuwenhoek. 2024 Mar 23;117(1):61. doi: 10.1007/s10482-024-01949-x.ABSTRACTYersinia pestis, the causative agent of plague, is a highly virulent bacterium that poses a significant threat to human health. Preserving this bacterium in a viable state is crucial for research and diagnostic purposes. This paper presents and evaluates a simple lyophilization protocol for the long-term storage of Y. pestis strains from Fiocruz-CYP, aiming to explore its impact on viability and long-term stability, while replacing the currently used methodologies. The lyophilization tests were conducted using the non-virulent Y. pestis...
Source: Antonie van Leeuwenhoek - March 23, 2024 Category: Microbiology Authors: Igor Vasconcelos Rocha Matheus Filgueira Bezerra Marise Sobreira Nilma Cintra Leal Alzira Maria Paiva de Almeida Source Type: research
Lyophilization for bacteria preservation: a promising approach for Yersinia pestis strains from an unique collection in Brazil (Fiocruz-CYP)
Antonie Van Leeuwenhoek. 2024 Mar 23;117(1):61. doi: 10.1007/s10482-024-01949-x.ABSTRACTYersinia pestis, the causative agent of plague, is a highly virulent bacterium that poses a significant threat to human health. Preserving this bacterium in a viable state is crucial for research and diagnostic purposes. This paper presents and evaluates a simple lyophilization protocol for the long-term storage of Y. pestis strains from Fiocruz-CYP, aiming to explore its impact on viability and long-term stability, while replacing the currently used methodologies. The lyophilization tests were conducted using the non-virulent Y. pestis...
Source: Antonie van Leeuwenhoek - March 23, 2024 Category: Microbiology Authors: Igor Vasconcelos Rocha Matheus Filgueira Bezerra Marise Sobreira Nilma Cintra Leal Alzira Maria Paiva de Almeida Source Type: research
Targeting methanotrophs and isolation of a novel psychrophilic Methylobacter species from a terrestrial Arctic alkaline methane seep in Lagoon Pingo, Central Spitsbergen (78 ° N)
Antonie Van Leeuwenhoek. 2024 Mar 22;117(1):60. doi: 10.1007/s10482-024-01953-1.ABSTRACTThe microbial diversity associated with terrestrial groundwater seepage through permafrost soils is tightly coupled to the geochemistry of these fluids. Terrestrial alkaline methane seeps from Lagoon Pingo, Central Spitsbergen (78°N) in Norway, with methane-saturated and oxygen-limited groundwater discharge providing a potential habitat for methanotrophy. Here, we report on the microbial community's comparative analyses and distribution patterns at two sites close to Lagoon Pingo's methane emission source. To target methane-oxidizing b...
Source: Antonie van Leeuwenhoek - March 22, 2024 Category: Microbiology Authors: Shalaka K Patil Tajul Islam Alexander Tveit Andrew Hodson Lise Øvreås Source Type: research
Targeting methanotrophs and isolation of a novel psychrophilic Methylobacter species from a terrestrial Arctic alkaline methane seep in Lagoon Pingo, Central Spitsbergen (78 ° N)
Antonie Van Leeuwenhoek. 2024 Mar 22;117(1):60. doi: 10.1007/s10482-024-01953-1.ABSTRACTThe microbial diversity associated with terrestrial groundwater seepage through permafrost soils is tightly coupled to the geochemistry of these fluids. Terrestrial alkaline methane seeps from Lagoon Pingo, Central Spitsbergen (78°N) in Norway, with methane-saturated and oxygen-limited groundwater discharge providing a potential habitat for methanotrophy. Here, we report on the microbial community's comparative analyses and distribution patterns at two sites close to Lagoon Pingo's methane emission source. To target methane-oxidizing b...
Source: Antonie van Leeuwenhoek - March 22, 2024 Category: Microbiology Authors: Shalaka K Patil Tajul Islam Alexander Tveit Andrew Hodson Lise Øvreås Source Type: research
Phylogenomic analysis of the Ponticus clade: strains isolated from the spotted rose snapper (Lutjanus guttatus)
Antonie Van Leeuwenhoek. 2024 Mar 20;117(1):59. doi: 10.1007/s10482-024-01959-9.ABSTRACTThe family Vibrionaceae is classified into many clades based on their phylogenetic relationships. The Ponticus clade is one of its clades and consists of four species, Vibrio panuliri, V. ponticus, V. rhodolitus, and V. taketomensis. Two strains, CAIM 703 and CAIM 1902, were isolated from the diseased spotted rose snapper external lesion (Lutjanus guttatus), they were analyzed to determine their taxonomic position, a phylogenetic analysis was performed based on the 16S rRNA sequences proved that the two strains are members of the genus ...
Source: Antonie van Leeuwenhoek - March 20, 2024 Category: Microbiology Authors: Alejandra de Jes ús Chavarín-Meza Bruno G ómez-Gil Adri án González-Castillo Source Type: research
Phylogenomic analysis of the Ponticus clade: strains isolated from the spotted rose snapper (Lutjanus guttatus)
Antonie Van Leeuwenhoek. 2024 Mar 20;117(1):59. doi: 10.1007/s10482-024-01959-9.ABSTRACTThe family Vibrionaceae is classified into many clades based on their phylogenetic relationships. The Ponticus clade is one of its clades and consists of four species, Vibrio panuliri, V. ponticus, V. rhodolitus, and V. taketomensis. Two strains, CAIM 703 and CAIM 1902, were isolated from the diseased spotted rose snapper external lesion (Lutjanus guttatus), they were analyzed to determine their taxonomic position, a phylogenetic analysis was performed based on the 16S rRNA sequences proved that the two strains are members of the genus ...
Source: Antonie van Leeuwenhoek - March 20, 2024 Category: Microbiology Authors: Alejandra de Jes ús Chavarín-Meza Bruno G ómez-Gil Adri án González-Castillo Source Type: research
The role of the Flb protein family in the life cycle of Aspergillus niger
Antonie Van Leeuwenhoek. 2024 Mar 19;117(1):58. doi: 10.1007/s10482-024-01957-x.ABSTRACTGenes flbA-E are involved in sporulation and vegetative growth in Aspergillus nidulans. Inactivation of either of these genes results in a fluffy phenotype with delayed or even abolished sporulation. Previously, a non-sporulating phenotype was obtained by inactivating flbA in Aspergillus niger, which was accompanied by lysis, thinner cell walls, and an increased secretome complexity. Here, we further studied the role of the flb genes of A. niger. Strains ΔflbA, ΔflbB and ΔflbE showed increased biomass formation, while inactivation of...
Source: Antonie van Leeuwenhoek - March 19, 2024 Category: Microbiology Authors: Xiaoyi Chen Juan P Moran Torres Han A B W östen Source Type: research
The role of the Flb protein family in the life cycle of Aspergillus niger
Antonie Van Leeuwenhoek. 2024 Mar 19;117(1):58. doi: 10.1007/s10482-024-01957-x.ABSTRACTGenes flbA-E are involved in sporulation and vegetative growth in Aspergillus nidulans. Inactivation of either of these genes results in a fluffy phenotype with delayed or even abolished sporulation. Previously, a non-sporulating phenotype was obtained by inactivating flbA in Aspergillus niger, which was accompanied by lysis, thinner cell walls, and an increased secretome complexity. Here, we further studied the role of the flb genes of A. niger. Strains ΔflbA, ΔflbB and ΔflbE showed increased biomass formation, while inactivation of...
Source: Antonie van Leeuwenhoek - March 19, 2024 Category: Microbiology Authors: Xiaoyi Chen Juan P Moran Torres Han A B W östen Source Type: research
Comparative genomics of an extensively drug resistant strain Klebsiella pneumoniae IITR008 with international high-risk clonal lineage ST147 isolated from river water
Antonie Van Leeuwenhoek. 2024 Mar 15;117(1):57. doi: 10.1007/s10482-024-01955-z.ABSTRACTCarbapenem resistant Klebsiella pneumoniae causing severe infection resulting in morbidity and mortality have become a global health concern. K. pneumoniae with sequence type ST147 is an international high-risk clonal lineage, genomic studies have been done on K. pneumoniae ST147 isolated from clinical origin but genomic data for environmental K. pneumoniae ST147 is very scarce. Herein, K. pneumoniae IITR008, an extensively drug resistant and potentially hypervirulent bacterium, was isolated from Triveni Sangam, the confluence of three ...
Source: Antonie van Leeuwenhoek - March 16, 2024 Category: Microbiology Authors: Deeksha Singh Shilpee Pal Srikrishna Subramanian Natesan Manickam Source Type: research
