Analytical Sciences : the international journal of the Japan Society for Analytical Chemistry This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Biotinylated cyclic naphthalene diimide as a searching tool for G4 sites on the genome
Anal Sci. 2024 Apr 12. doi: 10.1007/s44211-024-00551-5. Online ahead of print.ABSTRACTA biotinyl cyclic naphthalene diimide (biotinyl cNDI) (1), in which biotin is introduced on the cyclic linker chain of cNDI with high G-quadruplex (G4) specificity, was synthesized. 1 was used for binding analysis to G4 DNAs such as c-myc, c-kit, CEGF, or TA-core. The results showed that 1 bind to G4 DNAs with high affinity and, especially, two molecules of 1 bind to c-myc DNA from top and bottom of G4 site at K = 3.9 × 10-6 M-1 without changing the G4 structure. As a pulldown assay, 1 and streptavidin magnetic beads could be used to rec...
Source: Analytical Sciences : the international journal of the Japan Society for Analytical Chemistry - April 12, 2024 Category: Chemistry Authors: Satoshi Fujii Shinobu Sato Ryuki Hidaka Shigeori Takenaka Source Type: research

Fluorescent immunoassay for chloramphenicol based on the label-free polyadenine-mediated spherical nucleic acids triggered signal amplification
Anal Sci. 2024 Apr 12. doi: 10.1007/s44211-024-00565-z. Online ahead of print.ABSTRACTA fluorescent immunosorbent assay incorporating signal amplification away from the surface of spherical nucleic acid (SNA) was developed for the detection of chloramphenicol (CAP). Through the conjugation of antibodies and poly-adenine (polyA) DNA onto the surface of gold nanoparticles (AuNPs), the fabrication of the nano-immunoprobe was achieved in a more straightforward and cost-effective manner. Moreover, a strategy utilizing the hybridization chain reaction (HCR) in the amplification step was devised, with particular attention given t...
Source: Analytical Sciences : the international journal of the Japan Society for Analytical Chemistry - April 12, 2024 Category: Chemistry Authors: Weiwei Shen Qing Hong Xiang Huo Yijing Zhou Yahui Guo Zhenmin Liu Source Type: research

Smartphone-based imaging colorimetric assay for monitoring the quality of curcumin in turmeric powder
Anal Sci. 2024 Apr 12. doi: 10.1007/s44211-024-00562-2. Online ahead of print.ABSTRACTThis research developed a colorimetric assay for semi-quantitative curcumin detection. The screening test was performed using a ferric chloride to form a brownish color which was further used to evaluate the amount of curcumin in the turmeric powder samples. The quantitative assay was performed based on the color intensity of the curcumin target using a smartphone digital image colorimetry with a developed lightbox constructed with a white light-emitting diodes (LED) light source as the measurement device. Images in red, green, and blue (...
Source: Analytical Sciences : the international journal of the Japan Society for Analytical Chemistry - April 12, 2024 Category: Chemistry Authors: Jongjit Jantra Siriwan Teepoo Suchera Thananimit Source Type: research

Evaluation of the deteriorating effects of microbial primary metabolites on silk fibres
Anal Sci. 2024 Apr 12. doi: 10.1007/s44211-024-00568-w. Online ahead of print.ABSTRACTThe silk biodegradation process remains unclear and requires elucidation with advanced analytical tools. To address this challenge, the role of microbial primary metabolites in the deterioration of ancient silk was investigated using metabolomics and proteomics techniques in this work. The oxalic and palmitic acids were separately identified as the most abundant organic and fatty acid metabolites for silk-fabric deterioration via metabolomics. Proteomics showed that oxalic acid accelerated the degradation of silk proteins, revealing chang...
Source: Analytical Sciences : the international journal of the Japan Society for Analytical Chemistry - April 12, 2024 Category: Chemistry Authors: Chuanmiao Ding Lindan Pan Yichang Li Qianhao Jia Tao Wang Hao Du Bing Wang Source Type: research

Bridging biological samples to functional nucleic acid biosensor applications: current enzymatic-based strategies for single-stranded DNA generation
Anal Sci. 2024 Apr 12. doi: 10.1007/s44211-024-00566-y. Online ahead of print.ABSTRACTThe escalating threat of emerging diseases, often stemming from contaminants and lethal pathogens, has precipitated a heightened demand for sophisticated diagnostic tools. Within this landscape, the functional nucleic acid (FNA) biosensor, harnessing the power of single-stranded DNA (ssDNA), has emerged as a preeminent choice for target analyte detection. However, the dependence on ssDNA has raised difficulties in realizing it in biological samples. Therefore, the production of high-quality ssDNA from biological samples is critical. This ...
Source: Analytical Sciences : the international journal of the Japan Society for Analytical Chemistry - April 12, 2024 Category: Chemistry Authors: David Septian Sumanto Marpaung Ayu Oshin Yap Sinaga Damayanti Damayanti Taharuddin Taharuddin Source Type: research

Determination of low concentrations of glucose through colorimetric analysis using CoFe < sub > 2 < /sub > O < sub > 4 < /sub > magnetic catalyst and SAT-3
In this study, magnetic CoFe2O4 was prepared using a precipitation method as a natural enzyme mimetic. CoFe2O4 showed excellent peroxidase-like activity, as demonstrated by the Michaelis-Menten constant (Km) and the maximum velocity (Vmax). The linear ranges of the calibration curves for H2O2 and glucose were in the range of 0-500 µM, and the detection limits were 1.83 and 5.91 µM, respectively. This analytical method was applied for the determination of glucose in human serum, and the results were satisfactory and consistent with certified values. The performance of this sensor was comparable to or superior to those of ...
Source: Analytical Sciences : the international journal of the Japan Society for Analytical Chemistry - April 11, 2024 Category: Chemistry Authors: Kurumi Matsui Hideyuki Katsumata Mai Furukawa Ikki Tateishi Satoshi Kaneco Source Type: research

Colorimetric and fluorometric determination of uric acid by a suspension-based assay using enzyme-immobilized micro-sized particles
Anal Sci. 2024 Apr 10. doi: 10.1007/s44211-024-00556-0. Online ahead of print.ABSTRACTDaily monitoring of serum uric acid levels is very important to provide appropriate treatment according to the constitution and lifestyle of individual hyperuricemic patients. We have developed a suspension-based assay to measure uric acid by adding a sample solution to the suspension containing micro-sized particles immobilized on uricase and horseradish peroxidase (HRP). In the proposed method, the mediator reaction of uricase, HRP, and uric acid produces resorufin from Amplex red. This resorufin is adsorbed onto enzyme-immobilized micr...
Source: Analytical Sciences : the international journal of the Japan Society for Analytical Chemistry - April 10, 2024 Category: Chemistry Authors: Yukiko Moriiwa Keigo Hatakeyama Kazuhiro Morioka Yoshinori Inoue Hiroya Murakami Norio Teshima Akio Yanagida Atsushi Shoji Source Type: research

Development of a two-dimensional LC-MS/MS system for the determination of proline and 4-hydroxyproline enantiomers in biological and food samples
Anal Sci. 2024 Apr 10. doi: 10.1007/s44211-024-00530-w. Online ahead of print.ABSTRACTA two-dimensional LC-MS/MS system has been developed for the enantioselective determination of proline (Pro), cis-4-hydroxyproline (cis-4-Hyp) and trans-4-hydroxyproline (trans-4-Hyp) in a variety of biological samples. The amino acids were pre-column derivatized with 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F), and the NBD-derivatives were separated by a reversed-phase column (Singularity RP18) as their D plus L mixtures in the first dimension. The collected target fractions were then introduced into the second dimension where the enan...
Source: Analytical Sciences : the international journal of the Japan Society for Analytical Chemistry - April 10, 2024 Category: Chemistry Authors: Chiharu Ishii Yosuke Tojo Komei Iwasaki Akira Fujii Takeyuki Akita Masanobu Nagano Masashi Mita Tomomi Ide Kenji Hamase Source Type: research

Predicting the wicking rate of nitrocellulose membranes from recipe data: a case study using ANN at a membrane manufacturing in South Korea
Anal Sci. 2024 Apr 10. doi: 10.1007/s44211-024-00540-8. Online ahead of print.ABSTRACTLateral flow assays have been widely used for detecting coronavirus disease 2019 (COVID-19). A lateral flow assay consists of a Nitrocellulose (NC) membrane, which must have a specific lateral flow rate for the proteins to react. The wicking rate is conventionally used as a method to assess the lateral flow in membranes. We used multiple regression and artificial neural networks (ANN) to predict the wicking rate of NC membranes based on membrane recipe data. The developed ANN predicted the wicking rate with a mean square error of 0.059, w...
Source: Analytical Sciences : the international journal of the Japan Society for Analytical Chemistry - April 10, 2024 Category: Chemistry Authors: Janith Dissanayake Sung Bong Kang Jihoon Park Fang Yinbao Sungryul Park Min-Ho Lee Source Type: research

A compact microfluidic laser-induced fluorescence immunoassay system using avalanche photodiode for rapid detection of alpha-fetoprotein
Anal Sci. 2024 Apr 10. doi: 10.1007/s44211-024-00553-3. Online ahead of print.ABSTRACTAlpha-fetoprotein (AFP), commonly employed for early diagnosis of liver cancer, serves as a biomarker for cancer screening and diagnosis. Combining the high sensitivity and specificity of fluorescence immunoassay (FIA), developing a low-cost and efficient immunoassay system for AFP detection holds significant importance in disease diagnosis. In this work, we developed a miniaturized oblique laser-induced fluorescence (LIF) immunoassay system, coupled with a microfluidic PMMA/paper hybrid chip, for rapid detection of AFP. The system employ...
Source: Analytical Sciences : the international journal of the Japan Society for Analytical Chemistry - April 10, 2024 Category: Chemistry Authors: Chaowen Zheng Peng Dai Hui You Zhaokun Xian Wenyun Su Shixiong Wu Dong Xing Cuimin Sun Source Type: research

Elution behavior of drugs in high-speed counter-current chromatography using on-column complexation with metal ions
In this study, determination of (nitrogen containing) drugs by on-column complexation with metal ions in high-speed counter-current chromatography (HSCCC) was investigated. Bromazepam (BMP) was strongly retained in the organic upper stationary phase (UP) of the two-phase solvent system composed of tert-butyl methyl ether-acetonitrile-water (2:2:3, v/v/v) by eluting the aqueous lower mobile phase (LP) at a flow rate of 2 mL min-1. On the other hand, BMP (200 µg mL-1) was eluted faster without retention to the organic UP with the two-phase system containing 100 μg mL-1 of copper ions (CuCl2) because a very polar BMP-Cu2+ c...
Source: Analytical Sciences : the international journal of the Japan Society for Analytical Chemistry - April 9, 2024 Category: Chemistry Authors: Yukiko Moriiwa Atsushi Shoji Yoichi Shibusawa Akio Yanagida Source Type: research

Bioanalytical technologies using temperature-responsive polymers
Anal Sci. 2024 Apr 7. doi: 10.1007/s44211-024-00545-3. Online ahead of print.ABSTRACTIn recent decades, various bioanalytical technologies have been investigated for appropriate medical treatment and effective therapy. Temperature-responsive chromatography is a promising bioanalytical technology owing to its functional properties. Temperature-responsive chromatography uses a poly(N-isopropylacrylamide)(PNIPAAm) modified stationary phase as the column packing material. The hydrophobic interactions between PNIPAAm and the analyte could be modulated by changing the column temperature because of the temperature-responsive hydr...
Source: Analytical Sciences : the international journal of the Japan Society for Analytical Chemistry - April 7, 2024 Category: Chemistry Authors: Kenichi Nagase Source Type: research