Small airway fibroblasts from Chronic Obstructive Pulmonary Disease patients exhibit cellular senescence
CONCLUSIONS: These data suggest COPD SAF are senescent and may be associated with fibrotic properties and mitochondrial dysfunction. Further understanding cellular senescence in SAF may lead to potential therapies to limit SAD progression.PMID:38150543 | DOI:10.1152/ajplung.00419.2022 (Source: Am J Physiol Lung Ce...)
Source: Am J Physiol Lung Ce... - December 27, 2023 Category: Respiratory Medicine Authors: Catherine L Wrench Jonathan R Baker Sue Monkley Peter S Fenwick Lynne Murray Louise E Donnelly Peter J Barnes Source Type: research
Viral Airway Injury Promotes Cell Engraftment in an In Vitro Model of Cystic Fibrosis Cell Therapy
We examined competition dynamics by time-lapse confocal imaging and found that delivered cells accelerate airway regeneration by incorporating into the epithelium. Irradiating the repairing epithelium granted engrafting cells a competitive advantage by diminishing resident stem cell proliferation. Intentionally causing severe injury to the lungs of people with CF would be dangerous. However, naturally occurring events like viral infection can induce similar epithelial damage with patches of denuded epithelium. We found that viral preconditioning promoted effective engraftment of cells primed for viral resistance.PMID:38150...
Source: Am J Physiol Lung Ce... - December 27, 2023 Category: Respiratory Medicine Authors: Rhianna E Lee Teresa M Mascenik Sidra C Major Jacob R Galiger Emily Bulik-Sullivan Priscila F Siesser Catherine A Lewis James E Bear Jake A Le Suer Finn J Hawkins Raymond J Pickles Scott H Randell Source Type: research
Hemopexin Reverses Activation of Lung eIF2a and Decreases Mitochondrial Injury in Chlorine Exposed Mice
Am J Physiol Lung Cell Mol Physiol. 2023 Dec 27. doi: 10.1152/ajplung.00273.2023. Online ahead of print.ABSTRACTWe assessed the mechanisms by which non-encapsulated heme, released in the plasma of mice post exposure to chlorine (Cl2) gas, resulted in the initiation and propagation of acute lung injury. We exposed adult male and female C57BL/6 mice to Cl2 (500 ppm for 30 min), returned them to room air, and injected them intramuscularly with either human hemopexin (hHPX; 5 µg/ g BW in 50 µl saline) or vehicle at 1h post exposure. Upon return to room air, Cl2 exposed mice, injected with vehicle, developed respiratory acido...
Source: Am J Physiol Lung Ce... - December 27, 2023 Category: Respiratory Medicine Authors: Sadis Matalon Zhihong Yu Shubham Dubey Israr Ahmad Emily M Stephens Ammar Saadoon Alishlash Ashley Meyers Douglas Cossar Donald Stewart Edward P Acosta Kyoko Kojima Tamas Jilling James A Mobley Source Type: research
Does Vaping Increase the Likelihood of SARS-CoV-2 Infection? Paradoxically Yes and No
In conclusion, the study demonstrates that EC aerosols can impact susceptibility to SARS-CoV-2 infection depending on their specific ingredients. PG/VG alone or PG/VG plus nicotine enhanced infection through different mechanisms, while benzoic acid in JUUL{trade mark, serif} aerosols mitigated the increased infection caused by certain ingredients. These findings highlight the complex relationship between ECs and SARS-CoV-2 susceptibility, emphasizing the importance of considering the specific aerosol ingredients when evaluating the potential effects of ECs on infection risk.PMID:38147795 | DOI:10.1152/ajplung.00300.2022 (S...
Source: Am J Physiol Lung Ce... - December 26, 2023 Category: Respiratory Medicine Authors: Rattapol Phandthong Man Wong Ann Song Teresa Martinez Prue Talbot Source Type: research
Direct Thrombin Inhibitors Fail to Reverse the Negative Effects of Heparin on Lung Growth and Function after Murine Left Pneumonectomy
Am J Physiol Lung Cell Mol Physiol. 2023 Dec 19. doi: 10.1152/ajplung.00096.2023. Online ahead of print.ABSTRACTNeonates with congenital diaphragmatic hernia (CDH) frequently require cardiopulmonary bypass and systemic anticoagulation. We previously demonstrated that even subtherapeutic heparin impairs lung growth and function in a murine model of compensatory lung growth. The direct thrombin inhibitors (DTIs) bivalirudin and argatroban preserved growth in this model. While DTIs are increasingly used for systemic anticoagulation clinically, CDH patients may still receive heparin. In this experiment, lung endothelial cell p...
Source: Am J Physiol Lung Ce... - December 19, 2023 Category: Respiratory Medicine Authors: Savas T Tsikis Thomas I Hirsch Timothy Klouda Scott C Fligor Amy Pan Malachi M Joiner Sarah Z Wang Mikayla Quigley Angela Devietro Paul D Mitchell Hiroko Kishikawa Ke Yuan Mark Puder Source Type: research
Bacterial pneumonia-induced shedding of epithelial heparan sulfate inhibits the bactericidal activity of cathelicidin in a murine model
Am J Physiol Lung Cell Mol Physiol. 2023 Dec 19. doi: 10.1152/ajplung.00178.2023. Online ahead of print.ABSTRACTPrimary bacterial pneumonia is a common clinical syndrome leading to significant morbidity and mortality worldwide. In the current study, we investigate a novel, multidirectional relationship between the pulmonary epithelial glycocalyx and antimicrobial peptides in the setting of Methicillin Resistant Staphylococcus aureus (MRSA) pneumonia. Using an in vivo pneumonia model, we demonstrate that highly-sulfated heparan sulfate oligosaccharides (HS) are shed into the airspaces in response to MRSA pneumonia. In vitro...
Source: Am J Physiol Lung Ce... - December 19, 2023 Category: Respiratory Medicine Authors: Evan P Zehr Christopher L Erzen Kaori Oshima Christophe J Langou ët-Astrié Wells B LaRiviere Deling Shi Fuming Zhang Bruce D McCollister Samuel L Windham Alicia N Rizzo Julie A Bastarache Alexander R Horswill Eric P Schmidt Jakub M Kwiecinski James F Co Source Type: research
Direct Thrombin Inhibitors Fail to Reverse the Negative Effects of Heparin on Lung Growth and Function after Murine Left Pneumonectomy
Am J Physiol Lung Cell Mol Physiol. 2023 Dec 19. doi: 10.1152/ajplung.00096.2023. Online ahead of print.ABSTRACTNeonates with congenital diaphragmatic hernia (CDH) frequently require cardiopulmonary bypass and systemic anticoagulation. We previously demonstrated that even subtherapeutic heparin impairs lung growth and function in a murine model of compensatory lung growth. The direct thrombin inhibitors (DTIs) bivalirudin and argatroban preserved growth in this model. While DTIs are increasingly used for systemic anticoagulation clinically, CDH patients may still receive heparin. In this experiment, lung endothelial cell p...
Source: Am J Physiol Lung Ce... - December 19, 2023 Category: Respiratory Medicine Authors: Savas T Tsikis Thomas I Hirsch Timothy Klouda Scott C Fligor Amy Pan Malachi M Joiner Sarah Z Wang Mikayla Quigley Angela Devietro Paul D Mitchell Hiroko Kishikawa Ke Yuan Mark Puder Source Type: research
Bacterial pneumonia-induced shedding of epithelial heparan sulfate inhibits the bactericidal activity of cathelicidin in a murine model
Am J Physiol Lung Cell Mol Physiol. 2023 Dec 19. doi: 10.1152/ajplung.00178.2023. Online ahead of print.ABSTRACTPrimary bacterial pneumonia is a common clinical syndrome leading to significant morbidity and mortality worldwide. In the current study, we investigate a novel, multidirectional relationship between the pulmonary epithelial glycocalyx and antimicrobial peptides in the setting of Methicillin Resistant Staphylococcus aureus (MRSA) pneumonia. Using an in vivo pneumonia model, we demonstrate that highly-sulfated heparan sulfate oligosaccharides (HS) are shed into the airspaces in response to MRSA pneumonia. In vitro...
Source: Am J Physiol Lung Ce... - December 19, 2023 Category: Respiratory Medicine Authors: Evan P Zehr Christopher L Erzen Kaori Oshima Christophe J Langou ët-Astrié Wells B LaRiviere Deling Shi Fuming Zhang Bruce D McCollister Samuel L Windham Alicia N Rizzo Julie A Bastarache Alexander R Horswill Eric P Schmidt Jakub M Kwiecinski James F Co Source Type: research
Direct Thrombin Inhibitors Fail to Reverse the Negative Effects of Heparin on Lung Growth and Function after Murine Left Pneumonectomy
Am J Physiol Lung Cell Mol Physiol. 2023 Dec 19. doi: 10.1152/ajplung.00096.2023. Online ahead of print.ABSTRACTNeonates with congenital diaphragmatic hernia (CDH) frequently require cardiopulmonary bypass and systemic anticoagulation. We previously demonstrated that even subtherapeutic heparin impairs lung growth and function in a murine model of compensatory lung growth. The direct thrombin inhibitors (DTIs) bivalirudin and argatroban preserved growth in this model. While DTIs are increasingly used for systemic anticoagulation clinically, CDH patients may still receive heparin. In this experiment, lung endothelial cell p...
Source: Am J Physiol Lung Ce... - December 19, 2023 Category: Respiratory Medicine Authors: Savas T Tsikis Thomas I Hirsch Timothy Klouda Scott C Fligor Amy Pan Malachi M Joiner Sarah Z Wang Mikayla Quigley Angela Devietro Paul D Mitchell Hiroko Kishikawa Ke Yuan Mark Puder Source Type: research
Bacterial pneumonia-induced shedding of epithelial heparan sulfate inhibits the bactericidal activity of cathelicidin in a murine model
Am J Physiol Lung Cell Mol Physiol. 2023 Dec 19. doi: 10.1152/ajplung.00178.2023. Online ahead of print.ABSTRACTPrimary bacterial pneumonia is a common clinical syndrome leading to significant morbidity and mortality worldwide. In the current study, we investigate a novel, multidirectional relationship between the pulmonary epithelial glycocalyx and antimicrobial peptides in the setting of Methicillin Resistant Staphylococcus aureus (MRSA) pneumonia. Using an in vivo pneumonia model, we demonstrate that highly-sulfated heparan sulfate oligosaccharides (HS) are shed into the airspaces in response to MRSA pneumonia. In vitro...
Source: Am J Physiol Lung Ce... - December 19, 2023 Category: Respiratory Medicine Authors: Evan P Zehr Christopher L Erzen Kaori Oshima Christophe J Langou ët-Astrié Wells B LaRiviere Deling Shi Fuming Zhang Bruce D McCollister Samuel L Windham Alicia N Rizzo Julie A Bastarache Alexander R Horswill Eric P Schmidt Jakub M Kwiecinski James F Co Source Type: research
Direct Thrombin Inhibitors Fail to Reverse the Negative Effects of Heparin on Lung Growth and Function after Murine Left Pneumonectomy
Am J Physiol Lung Cell Mol Physiol. 2023 Dec 19. doi: 10.1152/ajplung.00096.2023. Online ahead of print.ABSTRACTNeonates with congenital diaphragmatic hernia (CDH) frequently require cardiopulmonary bypass and systemic anticoagulation. We previously demonstrated that even subtherapeutic heparin impairs lung growth and function in a murine model of compensatory lung growth. The direct thrombin inhibitors (DTIs) bivalirudin and argatroban preserved growth in this model. While DTIs are increasingly used for systemic anticoagulation clinically, CDH patients may still receive heparin. In this experiment, lung endothelial cell p...
Source: Am J Physiol Lung Ce... - December 19, 2023 Category: Respiratory Medicine Authors: Savas T Tsikis Thomas I Hirsch Timothy Klouda Scott C Fligor Amy Pan Malachi M Joiner Sarah Z Wang Mikayla Quigley Angela Devietro Paul D Mitchell Hiroko Kishikawa Ke Yuan Mark Puder Source Type: research
Bacterial pneumonia-induced shedding of epithelial heparan sulfate inhibits the bactericidal activity of cathelicidin in a murine model
Am J Physiol Lung Cell Mol Physiol. 2023 Dec 19. doi: 10.1152/ajplung.00178.2023. Online ahead of print.ABSTRACTPrimary bacterial pneumonia is a common clinical syndrome leading to significant morbidity and mortality worldwide. In the current study, we investigate a novel, multidirectional relationship between the pulmonary epithelial glycocalyx and antimicrobial peptides in the setting of Methicillin Resistant Staphylococcus aureus (MRSA) pneumonia. Using an in vivo pneumonia model, we demonstrate that highly-sulfated heparan sulfate oligosaccharides (HS) are shed into the airspaces in response to MRSA pneumonia. In vitro...
Source: Am J Physiol Lung Ce... - December 19, 2023 Category: Respiratory Medicine Authors: Evan P Zehr Christopher L Erzen Kaori Oshima Christophe J Langou ët-Astrié Wells B LaRiviere Deling Shi Fuming Zhang Bruce D McCollister Samuel L Windham Alicia N Rizzo Julie A Bastarache Alexander R Horswill Eric P Schmidt Jakub M Kwiecinski James F Co Source Type: research
Direct Thrombin Inhibitors Fail to Reverse the Negative Effects of Heparin on Lung Growth and Function after Murine Left Pneumonectomy
Am J Physiol Lung Cell Mol Physiol. 2023 Dec 19. doi: 10.1152/ajplung.00096.2023. Online ahead of print.ABSTRACTNeonates with congenital diaphragmatic hernia (CDH) frequently require cardiopulmonary bypass and systemic anticoagulation. We previously demonstrated that even subtherapeutic heparin impairs lung growth and function in a murine model of compensatory lung growth. The direct thrombin inhibitors (DTIs) bivalirudin and argatroban preserved growth in this model. While DTIs are increasingly used for systemic anticoagulation clinically, CDH patients may still receive heparin. In this experiment, lung endothelial cell p...
Source: Am J Physiol Lung Ce... - December 19, 2023 Category: Respiratory Medicine Authors: Savas T Tsikis Thomas I Hirsch Timothy Klouda Scott C Fligor Amy Pan Malachi M Joiner Sarah Z Wang Mikayla Quigley Angela Devietro Paul D Mitchell Hiroko Kishikawa Ke Yuan Mark Puder Source Type: research
Bacterial pneumonia-induced shedding of epithelial heparan sulfate inhibits the bactericidal activity of cathelicidin in a murine model
Am J Physiol Lung Cell Mol Physiol. 2023 Dec 19. doi: 10.1152/ajplung.00178.2023. Online ahead of print.ABSTRACTPrimary bacterial pneumonia is a common clinical syndrome leading to significant morbidity and mortality worldwide. In the current study, we investigate a novel, multidirectional relationship between the pulmonary epithelial glycocalyx and antimicrobial peptides in the setting of Methicillin Resistant Staphylococcus aureus (MRSA) pneumonia. Using an in vivo pneumonia model, we demonstrate that highly-sulfated heparan sulfate oligosaccharides (HS) are shed into the airspaces in response to MRSA pneumonia. In vitro...
Source: Am J Physiol Lung Ce... - December 19, 2023 Category: Respiratory Medicine Authors: Evan P Zehr Christopher L Erzen Kaori Oshima Christophe J Langou ët-Astrié Wells B LaRiviere Deling Shi Fuming Zhang Bruce D McCollister Samuel L Windham Alicia N Rizzo Julie A Bastarache Alexander R Horswill Eric P Schmidt Jakub M Kwiecinski James F Co Source Type: research
Direct Thrombin Inhibitors Fail to Reverse the Negative Effects of Heparin on Lung Growth and Function after Murine Left Pneumonectomy
Am J Physiol Lung Cell Mol Physiol. 2023 Dec 19. doi: 10.1152/ajplung.00096.2023. Online ahead of print.ABSTRACTNeonates with congenital diaphragmatic hernia (CDH) frequently require cardiopulmonary bypass and systemic anticoagulation. We previously demonstrated that even subtherapeutic heparin impairs lung growth and function in a murine model of compensatory lung growth. The direct thrombin inhibitors (DTIs) bivalirudin and argatroban preserved growth in this model. While DTIs are increasingly used for systemic anticoagulation clinically, CDH patients may still receive heparin. In this experiment, lung endothelial cell p...
Source: Am J Physiol Lung Ce... - December 19, 2023 Category: Respiratory Medicine Authors: Savas T Tsikis Thomas I Hirsch Timothy Klouda Scott C Fligor Amy Pan Malachi M Joiner Sarah Z Wang Mikayla Quigley Angela Devietro Paul D Mitchell Hiroko Kishikawa Ke Yuan Mark Puder Source Type: research
