Viral Airway Injury Promotes Cell Engraftment in an In Vitro Model of Cystic Fibrosis Cell Therapy
We examined competition dynamics by time-lapse confocal imaging and found that delivered cells accelerate airway regeneration by incorporating into the epithelium. Irradiating the repairing epithelium granted engrafting cells a competitive advantage by diminishing resident stem cell proliferation. Intentionally causing severe injury to the lungs of people with CF would be dangerous. However, naturally occurring events like viral infection can induce similar epithelial damage with patches of denuded epithelium. We found that viral preconditioning promoted effective engraftment of cells primed for viral resistance.PMID:38150...
Source: Am J Physiol Lung Ce... - December 27, 2023 Category: Respiratory Medicine Authors: Rhianna E Lee Teresa M Mascenik Sidra C Major Jacob R Galiger Emily Bulik-Sullivan Priscila F Siesser Catherine A Lewis James E Bear Jake A Le Suer Finn J Hawkins Raymond J Pickles Scott H Randell Source Type: research
Hemopexin Reverses Activation of Lung eIF2a and Decreases Mitochondrial Injury in Chlorine Exposed Mice
Am J Physiol Lung Cell Mol Physiol. 2023 Dec 27. doi: 10.1152/ajplung.00273.2023. Online ahead of print.ABSTRACTWe assessed the mechanisms by which non-encapsulated heme, released in the plasma of mice post exposure to chlorine (Cl2) gas, resulted in the initiation and propagation of acute lung injury. We exposed adult male and female C57BL/6 mice to Cl2 (500 ppm for 30 min), returned them to room air, and injected them intramuscularly with either human hemopexin (hHPX; 5 µg/ g BW in 50 µl saline) or vehicle at 1h post exposure. Upon return to room air, Cl2 exposed mice, injected with vehicle, developed respiratory acido...
Source: Am J Physiol Lung Ce... - December 27, 2023 Category: Respiratory Medicine Authors: Sadis Matalon Zhihong Yu Shubham Dubey Israr Ahmad Emily M Stephens Ammar Saadoon Alishlash Ashley Meyers Douglas Cossar Donald Stewart Edward P Acosta Kyoko Kojima Tamas Jilling James A Mobley Source Type: research
Small airway fibroblasts from Chronic Obstructive Pulmonary Disease patients exhibit cellular senescence
CONCLUSIONS: These data suggest COPD SAF are senescent and may be associated with fibrotic properties and mitochondrial dysfunction. Further understanding cellular senescence in SAF may lead to potential therapies to limit SAD progression.PMID:38150543 | DOI:10.1152/ajplung.00419.2022 (Source: Am J Physiol Lung Ce...)
Source: Am J Physiol Lung Ce... - December 27, 2023 Category: Respiratory Medicine Authors: Catherine L Wrench Jonathan R Baker Sue Monkley Peter S Fenwick Lynne Murray Louise E Donnelly Peter J Barnes Source Type: research
Viral Airway Injury Promotes Cell Engraftment in an In Vitro Model of Cystic Fibrosis Cell Therapy
We examined competition dynamics by time-lapse confocal imaging and found that delivered cells accelerate airway regeneration by incorporating into the epithelium. Irradiating the repairing epithelium granted engrafting cells a competitive advantage by diminishing resident stem cell proliferation. Intentionally causing severe injury to the lungs of people with CF would be dangerous. However, naturally occurring events like viral infection can induce similar epithelial damage with patches of denuded epithelium. We found that viral preconditioning promoted effective engraftment of cells primed for viral resistance.PMID:38150...
Source: Am J Physiol Lung Ce... - December 27, 2023 Category: Respiratory Medicine Authors: Rhianna E Lee Teresa M Mascenik Sidra C Major Jacob R Galiger Emily Bulik-Sullivan Priscila F Siesser Catherine A Lewis James E Bear Jake A Le Suer Finn J Hawkins Raymond J Pickles Scott H Randell Source Type: research
Hemopexin Reverses Activation of Lung eIF2a and Decreases Mitochondrial Injury in Chlorine Exposed Mice
Am J Physiol Lung Cell Mol Physiol. 2023 Dec 27. doi: 10.1152/ajplung.00273.2023. Online ahead of print.ABSTRACTWe assessed the mechanisms by which non-encapsulated heme, released in the plasma of mice post exposure to chlorine (Cl2) gas, resulted in the initiation and propagation of acute lung injury. We exposed adult male and female C57BL/6 mice to Cl2 (500 ppm for 30 min), returned them to room air, and injected them intramuscularly with either human hemopexin (hHPX; 5 µg/ g BW in 50 µl saline) or vehicle at 1h post exposure. Upon return to room air, Cl2 exposed mice, injected with vehicle, developed respiratory acido...
Source: Am J Physiol Lung Ce... - December 27, 2023 Category: Respiratory Medicine Authors: Sadis Matalon Zhihong Yu Shubham Dubey Israr Ahmad Emily M Stephens Ammar Saadoon Alishlash Ashley Meyers Douglas Cossar Donald Stewart Edward P Acosta Kyoko Kojima Tamas Jilling James A Mobley Source Type: research
Small airway fibroblasts from Chronic Obstructive Pulmonary Disease patients exhibit cellular senescence
CONCLUSIONS: These data suggest COPD SAF are senescent and may be associated with fibrotic properties and mitochondrial dysfunction. Further understanding cellular senescence in SAF may lead to potential therapies to limit SAD progression.PMID:38150543 | DOI:10.1152/ajplung.00419.2022 (Source: Am J Physiol Lung Ce...)
Source: Am J Physiol Lung Ce... - December 27, 2023 Category: Respiratory Medicine Authors: Catherine L Wrench Jonathan R Baker Sue Monkley Peter S Fenwick Lynne Murray Louise E Donnelly Peter J Barnes Source Type: research
Viral Airway Injury Promotes Cell Engraftment in an In Vitro Model of Cystic Fibrosis Cell Therapy
We examined competition dynamics by time-lapse confocal imaging and found that delivered cells accelerate airway regeneration by incorporating into the epithelium. Irradiating the repairing epithelium granted engrafting cells a competitive advantage by diminishing resident stem cell proliferation. Intentionally causing severe injury to the lungs of people with CF would be dangerous. However, naturally occurring events like viral infection can induce similar epithelial damage with patches of denuded epithelium. We found that viral preconditioning promoted effective engraftment of cells primed for viral resistance.PMID:38150...
Source: Am J Physiol Lung Ce... - December 27, 2023 Category: Respiratory Medicine Authors: Rhianna E Lee Teresa M Mascenik Sidra C Major Jacob R Galiger Emily Bulik-Sullivan Priscila F Siesser Catherine A Lewis James E Bear Jake A Le Suer Finn J Hawkins Raymond J Pickles Scott H Randell Source Type: research
Hemopexin Reverses Activation of Lung eIF2a and Decreases Mitochondrial Injury in Chlorine Exposed Mice
Am J Physiol Lung Cell Mol Physiol. 2023 Dec 27. doi: 10.1152/ajplung.00273.2023. Online ahead of print.ABSTRACTWe assessed the mechanisms by which non-encapsulated heme, released in the plasma of mice post exposure to chlorine (Cl2) gas, resulted in the initiation and propagation of acute lung injury. We exposed adult male and female C57BL/6 mice to Cl2 (500 ppm for 30 min), returned them to room air, and injected them intramuscularly with either human hemopexin (hHPX; 5 µg/ g BW in 50 µl saline) or vehicle at 1h post exposure. Upon return to room air, Cl2 exposed mice, injected with vehicle, developed respiratory acido...
Source: Am J Physiol Lung Ce... - December 27, 2023 Category: Respiratory Medicine Authors: Sadis Matalon Zhihong Yu Shubham Dubey Israr Ahmad Emily M Stephens Ammar Saadoon Alishlash Ashley Meyers Douglas Cossar Donald Stewart Edward P Acosta Kyoko Kojima Tamas Jilling James A Mobley Source Type: research
Small airway fibroblasts from Chronic Obstructive Pulmonary Disease patients exhibit cellular senescence
CONCLUSIONS: These data suggest COPD SAF are senescent and may be associated with fibrotic properties and mitochondrial dysfunction. Further understanding cellular senescence in SAF may lead to potential therapies to limit SAD progression.PMID:38150543 | DOI:10.1152/ajplung.00419.2022 (Source: Am J Physiol Lung Ce...)
Source: Am J Physiol Lung Ce... - December 27, 2023 Category: Respiratory Medicine Authors: Catherine L Wrench Jonathan R Baker Sue Monkley Peter S Fenwick Lynne Murray Louise E Donnelly Peter J Barnes Source Type: research
Viral Airway Injury Promotes Cell Engraftment in an In Vitro Model of Cystic Fibrosis Cell Therapy
We examined competition dynamics by time-lapse confocal imaging and found that delivered cells accelerate airway regeneration by incorporating into the epithelium. Irradiating the repairing epithelium granted engrafting cells a competitive advantage by diminishing resident stem cell proliferation. Intentionally causing severe injury to the lungs of people with CF would be dangerous. However, naturally occurring events like viral infection can induce similar epithelial damage with patches of denuded epithelium. We found that viral preconditioning promoted effective engraftment of cells primed for viral resistance.PMID:38150...
Source: Am J Physiol Lung Ce... - December 27, 2023 Category: Respiratory Medicine Authors: Rhianna E Lee Teresa M Mascenik Sidra C Major Jacob R Galiger Emily Bulik-Sullivan Priscila F Siesser Catherine A Lewis James E Bear Jake A Le Suer Finn J Hawkins Raymond J Pickles Scott H Randell Source Type: research
Hemopexin Reverses Activation of Lung eIF2a and Decreases Mitochondrial Injury in Chlorine Exposed Mice
Am J Physiol Lung Cell Mol Physiol. 2023 Dec 27. doi: 10.1152/ajplung.00273.2023. Online ahead of print.ABSTRACTWe assessed the mechanisms by which non-encapsulated heme, released in the plasma of mice post exposure to chlorine (Cl2) gas, resulted in the initiation and propagation of acute lung injury. We exposed adult male and female C57BL/6 mice to Cl2 (500 ppm for 30 min), returned them to room air, and injected them intramuscularly with either human hemopexin (hHPX; 5 µg/ g BW in 50 µl saline) or vehicle at 1h post exposure. Upon return to room air, Cl2 exposed mice, injected with vehicle, developed respiratory acido...
Source: Am J Physiol Lung Ce... - December 27, 2023 Category: Respiratory Medicine Authors: Sadis Matalon Zhihong Yu Shubham Dubey Israr Ahmad Emily M Stephens Ammar Saadoon Alishlash Ashley Meyers Douglas Cossar Donald Stewart Edward P Acosta Kyoko Kojima Tamas Jilling James A Mobley Source Type: research
Small airway fibroblasts from Chronic Obstructive Pulmonary Disease patients exhibit cellular senescence
CONCLUSIONS: These data suggest COPD SAF are senescent and may be associated with fibrotic properties and mitochondrial dysfunction. Further understanding cellular senescence in SAF may lead to potential therapies to limit SAD progression.PMID:38150543 | DOI:10.1152/ajplung.00419.2022 (Source: Am J Physiol Lung Ce...)
Source: Am J Physiol Lung Ce... - December 27, 2023 Category: Respiratory Medicine Authors: Catherine L Wrench Jonathan R Baker Sue Monkley Peter S Fenwick Lynne Murray Louise E Donnelly Peter J Barnes Source Type: research
Viral Airway Injury Promotes Cell Engraftment in an In Vitro Model of Cystic Fibrosis Cell Therapy
We examined competition dynamics by time-lapse confocal imaging and found that delivered cells accelerate airway regeneration by incorporating into the epithelium. Irradiating the repairing epithelium granted engrafting cells a competitive advantage by diminishing resident stem cell proliferation. Intentionally causing severe injury to the lungs of people with CF would be dangerous. However, naturally occurring events like viral infection can induce similar epithelial damage with patches of denuded epithelium. We found that viral preconditioning promoted effective engraftment of cells primed for viral resistance.PMID:38150...
Source: Am J Physiol Lung Ce... - December 27, 2023 Category: Respiratory Medicine Authors: Rhianna E Lee Teresa M Mascenik Sidra C Major Jacob R Galiger Emily Bulik-Sullivan Priscila F Siesser Catherine A Lewis James E Bear Jake A Le Suer Finn J Hawkins Raymond J Pickles Scott H Randell Source Type: research
Hemopexin Reverses Activation of Lung eIF2a and Decreases Mitochondrial Injury in Chlorine Exposed Mice
Am J Physiol Lung Cell Mol Physiol. 2023 Dec 27. doi: 10.1152/ajplung.00273.2023. Online ahead of print.ABSTRACTWe assessed the mechanisms by which non-encapsulated heme, released in the plasma of mice post exposure to chlorine (Cl2) gas, resulted in the initiation and propagation of acute lung injury. We exposed adult male and female C57BL/6 mice to Cl2 (500 ppm for 30 min), returned them to room air, and injected them intramuscularly with either human hemopexin (hHPX; 5 µg/ g BW in 50 µl saline) or vehicle at 1h post exposure. Upon return to room air, Cl2 exposed mice, injected with vehicle, developed respiratory acido...
Source: Am J Physiol Lung Ce... - December 27, 2023 Category: Respiratory Medicine Authors: Sadis Matalon Zhihong Yu Shubham Dubey Israr Ahmad Emily M Stephens Ammar Saadoon Alishlash Ashley Meyers Douglas Cossar Donald Stewart Edward P Acosta Kyoko Kojima Tamas Jilling James A Mobley Source Type: research
Small airway fibroblasts from Chronic Obstructive Pulmonary Disease patients exhibit cellular senescence
CONCLUSIONS: These data suggest COPD SAF are senescent and may be associated with fibrotic properties and mitochondrial dysfunction. Further understanding cellular senescence in SAF may lead to potential therapies to limit SAD progression.PMID:38150543 | DOI:10.1152/ajplung.00419.2022 (Source: Am J Physiol Lung Ce...)
Source: Am J Physiol Lung Ce... - December 27, 2023 Category: Respiratory Medicine Authors: Catherine L Wrench Jonathan R Baker Sue Monkley Peter S Fenwick Lynne Murray Louise E Donnelly Peter J Barnes Source Type: research
