A Novel Interaction Between Aquaporin 1 and Caspase-3 in Pulmonary Arterial Smooth Muscle Cells
Am J Physiol Lung Cell Mol Physiol. 2024 Feb 20. doi: 10.1152/ajplung.00017.2024. Online ahead of print.ABSTRACTPulmonary hypertension (PH) is a condition in which remodeling of the pulmonary vasculature leads to hypertrophy of the muscular vascular wall and extension of muscle into non-muscular arteries. These pathologic changes are predominantly due to abnormal proliferation and migration of pulmonary arterial smooth muscle cells (PASMCs), enhanced cellular functions that have been linked to increases in the cell membrane protein aquaporin-1 (AQP1). However, the mechanisms underlying increased AQP1 abundance have not bee...
Source: Am J Physiol Lung Ce... - February 20, 2024 Category: Respiratory Medicine Authors: Shannon Niedermeyer Xin Yun Marielena Trujillo Haiyang Jiang Manuella R Andrade Todd M Kolb Karthik Suresh Mahendra Damarla Larissa A Shimoda Source Type: research
Preservation of Recipient Plasma Sphingosine-1-Phosphate Levels Reduces Transfusion-related Acute Lung Injury
Am J Physiol Lung Cell Mol Physiol. 2024 Feb 20. doi: 10.1152/ajplung.00388.2023. Online ahead of print.ABSTRACTCold stored platelets (CS) are once again being reintroduced for clinical use. Transfused CS offer benefits over room temperature stored platelets (RTS) such as increased hemostatic effects and prolongation of shelf-life. Despite these advantages little is known about their association with transfusion-related acute lung injury (TRALI). TRALI is associated with prolonged storage RTS and has a mortality of > 15%. Determining the safety of CS is important considering their proposed use in TRALI-vulnerable popula...
Source: Am J Physiol Lung Ce... - February 20, 2024 Category: Respiratory Medicine Authors: Vanessa Hsing Han Qi Zhao Martin Post Dana Devine Mark J McVey Source Type: research
Multivalent, calcium-independent binding of surfactant protein A and D to sulfated glycosaminoglycans of the alveolar epithelial glycocalyx
Am J Physiol Lung Cell Mol Physiol. 2024 Feb 20. doi: 10.1152/ajplung.00283.2023. Online ahead of print.ABSTRACTLung surfactant collectins, surfactant protein A (SP-A) and D (SP-D), are oligomeric C-type lectins involved in lung immunity. Through their carbohydrate recognition domain, they recognize carbohydrates at pathogen surfaces and initiate lung innate immune response. Here, we propose that they may also be able to bind to other carbohydrates present in typical cell surfaces, such as the alveolar epithelial glycocalyx. To test this hypothesis, we analyzed and quantified the binding affinity of SP-A and SP-D to differ...
Source: Am J Physiol Lung Ce... - February 20, 2024 Category: Respiratory Medicine Authors: Rabia Avcibas Anna Vermul Vladimir Gluhovic Nico Boback Raquel Arroyo Paul S Kingma Miriam Isasi-Campillo Lucia Garcia-Ortega Matthias Griese Wolfgang M Kuebler Matthias Ochs Daniel Lauster Elena Lopez-Rodriguez Source Type: research
Developmental changes in lung function of mice are independent of sex as a biological variable
Am J Physiol Lung Cell Mol Physiol. 2024 Feb 20. doi: 10.1152/ajplung.00120.2023. Online ahead of print.ABSTRACTPulmonary function testing (PFT) in mice includes biomechanical assessment of lung function relevant to physiology in health and its alteration in disease, hence, it is frequently used in preclinical modeling of human lung pathologies. Despite numerous reports of PFT in mice of various ages, there is a lack of reference data for developing mice collected using consistent methods. Therefore, we profiled PFTs in male and female C57BL/6J mice from 2 to 23 weeks of age, providing reference values for age- and sex-dep...
Source: Am J Physiol Lung Ce... - February 20, 2024 Category: Respiratory Medicine Authors: Thomas B ärnthaler Abhay B Ramachandra Sad è Ebanks Nicole Guerrera Lokesh Sharma Charles S Dela Cruz Jay D Humphrey Edward P Manning Source Type: research
Anti-Inflammatory Roles of Type I Interferon Signaling in the Lung
In this study, we investigated the role of type I interferon signaling in the inflammatory response against diverse clinically relevant stimuli. Using mice deficient in type I interferon signaling (IFNAR1-/-), we demonstrate that the absence of interferon signaling resulted in a robust and persistent inflammatory response against Pseudomonas aeruginosa, lipopolysaccharide, and chemotherapeutic agent bleomycin. The elevated inflammatory response in IFNAR1-/- mice was manifested as elevated myeloid cells such as macrophages and neutrophils, in the broncho-alveolar lavage. The inflammatory cell response in the IFNAR1-/- mice ...
Source: Am J Physiol Lung Ce... - February 20, 2024 Category: Respiratory Medicine Authors: Jingjing Feng Yi Liu Jooyoung Kim Farida Ahangari Naftali Kaminski William G Bain Zhijun Jie Charles S Dela Cruz Lokesh Sharma Source Type: research
A Novel Interaction Between Aquaporin 1 and Caspase-3 in Pulmonary Arterial Smooth Muscle Cells
Am J Physiol Lung Cell Mol Physiol. 2024 Feb 20. doi: 10.1152/ajplung.00017.2024. Online ahead of print.ABSTRACTPulmonary hypertension (PH) is a condition in which remodeling of the pulmonary vasculature leads to hypertrophy of the muscular vascular wall and extension of muscle into non-muscular arteries. These pathologic changes are predominantly due to abnormal proliferation and migration of pulmonary arterial smooth muscle cells (PASMCs), enhanced cellular functions that have been linked to increases in the cell membrane protein aquaporin-1 (AQP1). However, the mechanisms underlying increased AQP1 abundance have not bee...
Source: Am J Physiol Lung Ce... - February 20, 2024 Category: Respiratory Medicine Authors: Shannon Niedermeyer Xin Yun Marielena Trujillo Haiyang Jiang Manuella R Andrade Todd M Kolb Karthik Suresh Mahendra Damarla Larissa A Shimoda Source Type: research
Preservation of Recipient Plasma Sphingosine-1-Phosphate Levels Reduces Transfusion-related Acute Lung Injury
Am J Physiol Lung Cell Mol Physiol. 2024 Feb 20. doi: 10.1152/ajplung.00388.2023. Online ahead of print.ABSTRACTCold stored platelets (CS) are once again being reintroduced for clinical use. Transfused CS offer benefits over room temperature stored platelets (RTS) such as increased hemostatic effects and prolongation of shelf-life. Despite these advantages little is known about their association with transfusion-related acute lung injury (TRALI). TRALI is associated with prolonged storage RTS and has a mortality of > 15%. Determining the safety of CS is important considering their proposed use in TRALI-vulnerable popula...
Source: Am J Physiol Lung Ce... - February 20, 2024 Category: Respiratory Medicine Authors: Vanessa Hsing Han Qi Zhao Martin Post Dana Devine Mark J McVey Source Type: research
Multivalent, calcium-independent binding of surfactant protein A and D to sulfated glycosaminoglycans of the alveolar epithelial glycocalyx
Am J Physiol Lung Cell Mol Physiol. 2024 Feb 20. doi: 10.1152/ajplung.00283.2023. Online ahead of print.ABSTRACTLung surfactant collectins, surfactant protein A (SP-A) and D (SP-D), are oligomeric C-type lectins involved in lung immunity. Through their carbohydrate recognition domain, they recognize carbohydrates at pathogen surfaces and initiate lung innate immune response. Here, we propose that they may also be able to bind to other carbohydrates present in typical cell surfaces, such as the alveolar epithelial glycocalyx. To test this hypothesis, we analyzed and quantified the binding affinity of SP-A and SP-D to differ...
Source: Am J Physiol Lung Ce... - February 20, 2024 Category: Respiratory Medicine Authors: Rabia Avcibas Anna Vermul Vladimir Gluhovic Nico Boback Raquel Arroyo Paul S Kingma Miriam Isasi-Campillo Lucia Garcia-Ortega Matthias Griese Wolfgang M Kuebler Matthias Ochs Daniel Lauster Elena Lopez-Rodriguez Source Type: research
Developmental changes in lung function of mice are independent of sex as a biological variable
Am J Physiol Lung Cell Mol Physiol. 2024 Feb 20. doi: 10.1152/ajplung.00120.2023. Online ahead of print.ABSTRACTPulmonary function testing (PFT) in mice includes biomechanical assessment of lung function relevant to physiology in health and its alteration in disease, hence, it is frequently used in preclinical modeling of human lung pathologies. Despite numerous reports of PFT in mice of various ages, there is a lack of reference data for developing mice collected using consistent methods. Therefore, we profiled PFTs in male and female C57BL/6J mice from 2 to 23 weeks of age, providing reference values for age- and sex-dep...
Source: Am J Physiol Lung Ce... - February 20, 2024 Category: Respiratory Medicine Authors: Thomas B ärnthaler Abhay B Ramachandra Sad è Ebanks Nicole Guerrera Lokesh Sharma Charles S Dela Cruz Jay D Humphrey Edward P Manning Source Type: research
Anti-Inflammatory Roles of Type I Interferon Signaling in the Lung
In this study, we investigated the role of type I interferon signaling in the inflammatory response against diverse clinically relevant stimuli. Using mice deficient in type I interferon signaling (IFNAR1-/-), we demonstrate that the absence of interferon signaling resulted in a robust and persistent inflammatory response against Pseudomonas aeruginosa, lipopolysaccharide, and chemotherapeutic agent bleomycin. The elevated inflammatory response in IFNAR1-/- mice was manifested as elevated myeloid cells such as macrophages and neutrophils, in the broncho-alveolar lavage. The inflammatory cell response in the IFNAR1-/- mice ...
Source: Am J Physiol Lung Ce... - February 20, 2024 Category: Respiratory Medicine Authors: Jingjing Feng Yi Liu Jooyoung Kim Farida Ahangari Naftali Kaminski William G Bain Zhijun Jie Charles S Dela Cruz Lokesh Sharma Source Type: research
A Novel Interaction Between Aquaporin 1 and Caspase-3 in Pulmonary Arterial Smooth Muscle Cells
Am J Physiol Lung Cell Mol Physiol. 2024 Feb 20. doi: 10.1152/ajplung.00017.2024. Online ahead of print.ABSTRACTPulmonary hypertension (PH) is a condition in which remodeling of the pulmonary vasculature leads to hypertrophy of the muscular vascular wall and extension of muscle into non-muscular arteries. These pathologic changes are predominantly due to abnormal proliferation and migration of pulmonary arterial smooth muscle cells (PASMCs), enhanced cellular functions that have been linked to increases in the cell membrane protein aquaporin-1 (AQP1). However, the mechanisms underlying increased AQP1 abundance have not bee...
Source: Am J Physiol Lung Ce... - February 20, 2024 Category: Respiratory Medicine Authors: Shannon Niedermeyer Xin Yun Marielena Trujillo Haiyang Jiang Manuella R Andrade Todd M Kolb Karthik Suresh Mahendra Damarla Larissa A Shimoda Source Type: research
Preservation of Recipient Plasma Sphingosine-1-Phosphate Levels Reduces Transfusion-related Acute Lung Injury
Am J Physiol Lung Cell Mol Physiol. 2024 Feb 20. doi: 10.1152/ajplung.00388.2023. Online ahead of print.ABSTRACTCold stored platelets (CS) are once again being reintroduced for clinical use. Transfused CS offer benefits over room temperature stored platelets (RTS) such as increased hemostatic effects and prolongation of shelf-life. Despite these advantages little is known about their association with transfusion-related acute lung injury (TRALI). TRALI is associated with prolonged storage RTS and has a mortality of > 15%. Determining the safety of CS is important considering their proposed use in TRALI-vulnerable popula...
Source: Am J Physiol Lung Ce... - February 20, 2024 Category: Respiratory Medicine Authors: Vanessa Hsing Han Qi Zhao Martin Post Dana Devine Mark J McVey Source Type: research
Multivalent, calcium-independent binding of surfactant protein A and D to sulfated glycosaminoglycans of the alveolar epithelial glycocalyx
Am J Physiol Lung Cell Mol Physiol. 2024 Feb 20. doi: 10.1152/ajplung.00283.2023. Online ahead of print.ABSTRACTLung surfactant collectins, surfactant protein A (SP-A) and D (SP-D), are oligomeric C-type lectins involved in lung immunity. Through their carbohydrate recognition domain, they recognize carbohydrates at pathogen surfaces and initiate lung innate immune response. Here, we propose that they may also be able to bind to other carbohydrates present in typical cell surfaces, such as the alveolar epithelial glycocalyx. To test this hypothesis, we analyzed and quantified the binding affinity of SP-A and SP-D to differ...
Source: Am J Physiol Lung Ce... - February 20, 2024 Category: Respiratory Medicine Authors: Rabia Avcibas Anna Vermul Vladimir Gluhovic Nico Boback Raquel Arroyo Paul S Kingma Miriam Isasi-Campillo Lucia Garcia-Ortega Matthias Griese Wolfgang M Kuebler Matthias Ochs Daniel Lauster Elena Lopez-Rodriguez Source Type: research
Developmental changes in lung function of mice are independent of sex as a biological variable
Am J Physiol Lung Cell Mol Physiol. 2024 Feb 20. doi: 10.1152/ajplung.00120.2023. Online ahead of print.ABSTRACTPulmonary function testing (PFT) in mice includes biomechanical assessment of lung function relevant to physiology in health and its alteration in disease, hence, it is frequently used in preclinical modeling of human lung pathologies. Despite numerous reports of PFT in mice of various ages, there is a lack of reference data for developing mice collected using consistent methods. Therefore, we profiled PFTs in male and female C57BL/6J mice from 2 to 23 weeks of age, providing reference values for age- and sex-dep...
Source: Am J Physiol Lung Ce... - February 20, 2024 Category: Respiratory Medicine Authors: Thomas B ärnthaler Abhay B Ramachandra Sad è Ebanks Nicole Guerrera Lokesh Sharma Charles S Dela Cruz Jay D Humphrey Edward P Manning Source Type: research
Anti-Inflammatory Roles of Type I Interferon Signaling in the Lung
In this study, we investigated the role of type I interferon signaling in the inflammatory response against diverse clinically relevant stimuli. Using mice deficient in type I interferon signaling (IFNAR1-/-), we demonstrate that the absence of interferon signaling resulted in a robust and persistent inflammatory response against Pseudomonas aeruginosa, lipopolysaccharide, and chemotherapeutic agent bleomycin. The elevated inflammatory response in IFNAR1-/- mice was manifested as elevated myeloid cells such as macrophages and neutrophils, in the broncho-alveolar lavage. The inflammatory cell response in the IFNAR1-/- mice ...
Source: Am J Physiol Lung Ce... - February 20, 2024 Category: Respiratory Medicine Authors: Jingjing Feng Yi Liu Jooyoung Kim Farida Ahangari Naftali Kaminski William G Bain Zhijun Jie Charles S Dela Cruz Lokesh Sharma Source Type: research
