Investigation of Enzyme Inhibitory, Antioxidant Activities and Chemical Properties of Pistacia vera Leaves by Using LC-QTOF-MS and RP-HPLC

Since the leaves of some Pistacia species are used in traditional folk medicine for diabetes, the in vitro antidiabetic effect (α-glucosidase andα-amylase) of Pistacia vera leaves, as well as the antihypercholesterolemic (cholesterol esterase), antiobesity (pancreatic lipase) and antioxidant activities (total antioxidant capacity, DPPH radical scavenging activity, metal chelating activity and ferric reducing antioxidant power) of P. vera leaves, were investigated. Aqueous alcoholic leaf extract inhibitedα-amylase,α-glucosidase, and pancreatic lipase with 7.74± 0.72, 11.08± 3.96, and 168.43± 26.10µg/mL IC50 values, respectively. It was determined that the crude extract had high DPPH radical scavenging activity, ferric reducing power and moderate metal chelating activity. The ethyl acetate sub-extract obtained by liquid-liquid fractionation of the crude extract showed potentα-amylase andα-glucosidase inhibitory activity. Ethyl acetate sub-extract (5.794± 0.027 g/100 g sub-extract) was standardized by RP-HPLC on the basis ofβ-pentagalloyl glucose, which showed inhibitory effect on both amylase and glucosidase enzyme. Fifteen compounds, seven of which are organic acid derivatives and eight of which are flavonoids, were identified by LC-QTOF-MS analysis in the crude extract of P. vera leaves. Seven of the fifteen phenolic compounds detected in the crude extrac...
Source: Iranian Journal of Pharmaceutical Research - Category: Drugs & Pharmacology Source Type: research