Protective effect of Tilia americana var. mexicana against kainic acid-induced damage in the brain, liver and kidney: behavioural and biochemical changes

Tilia americana var. mexicana possesses anticonvulsant, antioxidant, neuroprotective and hepa-toprotective activities. The spectrum of anticonvulsant activity in status epilepticus models has not been sufficiently explored. We evaluated the effects of ethyl acetate (EAc) and methanol (ME) extracts on kainic acid (KA)-induced seizures by measuring behaviour (severity and laten-cy) and lipoperoxidation levels in different brain areas (cerebellum, brain hemispheres, cortex, medulla), the kidney and the liver in rats. Wistar male rats were administered KA (10 mg/kg, i.p.) after 3 days of pretreatment with Tilia extract (100 mg/kg). EAc and ME Tilia extracts signifi-cantly decreased the severity of phase 1 and phase 2 seizures, respectively. The ME Tilia extract increased the latency to seizure (27±2 min) compared to the control (13±2 min). ME and EAc Tilia extracts significantly prevented the increase in lipid peroxidation caused by KA-induced seizures in the cerebellum, brain hemispheres, cortex, medulla, liver and kidney. The vehicle olive oil (OO) also showed anticonvulsant effects, decreasing the severity of seizures to phase 3 and decreasing the lipoperoxidation levels in the cerebellum, brain hemispheres, cortex, medulla, liver and kidney. The anticonvulsant activity of Tilia is mediated by antioxidant effects in central and systemic areas that involve synergistic interactions among the chemical constituents of these ex-tracts (glucosides of quercetin ...
Source: Iranian Journal of Pharmaceutical Research - Category: Drugs & Pharmacology Source Type: research