In vitro anthelmintic activity of Dennettia tripetala G. Baker (Annonaceae) Fruits against Haemonchus contortus

This study was aimed to investigate the in vitro anthelmintic activity ofDennettia tripetala G. Baker (Annonaceae) fruits againstHaemonchus contortus. Using in vitro techniques, the anthelmintic activity of extracts and fractions ofD. tripetala G. Baker (Annonaceae) was evaluated for ovicidal (Egg hatch inhibition test) and larvicidal (larval mortality test) activity. Besides, the maximum tolerated dose was determined in adult albino rats administered, 300, 400, and 500  mg/kg body weight of the CME fraction, and observed over a period of 48 h for signs of toxicity and mortality. Phytochemical screening uncovered the occurrence of flavonoids, steroids/triterpenes, cardiac glycosides, saponins, tannins, carbohydrates, and alkaloids in the crude methanol extract (C ME), the ethyl acetate fraction (EAF), and butanol fraction. The maximum tolerated dose of the CME ofD. tripetala did not produce observable signs of toxicity or death in all the rats given up to 500  mg/kg. The CME and EAF ofD. tripetala fruits produced a significant (\(p< 0.05\)) reduction in the hatchability ofH. contortus eggs in a concentration-dependent manner, while the CME at concentrations between 12.5 and 100  mg/ml completely inhibited the hatching ofH. contortus eggs. Similarly, EAF at doses of 25, 50, and 100  mg/ml completely inhibited the hatching ofH. contortus eggs. The CME and EAF ofD. tripetala fruits produced significant (\(p< 0.05\)) larvicidal activity against L3 ofH. contortus in a c...
Source: Journal of Parasitic Diseases - Category: Parasitology Source Type: research