Determining the Prevalence of Norovirus Infection in Diarrheal Patients and Evaluation of Different Diagnostic Methods

In this study it was aimed to determine the prevalence of norovirus and other gastroenteritis causative viruses in diarrheal patients and determine the sensitivity and specificity rates of EIA and ICG methods in sporadic cases by the chosen gold standard molecular reference method real-time reverse transcriptase polymerase chain reaction (rRT-PCR). In this study, 184 stool samples that met the study criteria and sent to İstanbul University İstanbul Faculty of Medicine Medical Microbiology Laboratory for routine bacteriological culture between January-July 2018 were included. All samples were evaluated with diagnostic kits BD MAX Enteric Viral Panel (Becton Dickinson, Canada) for rRT-PCR, RIDASCREEN Norovirus 3rd Generation (C1401) (R-Biopharm, Germany) for EIA, RIDAQUICK Norovirus Test (N1402) (R-Biopharm, Germany) for ICG, according to the manufacturer instructions. In terms of the presence of norovirus in 184 stool samples, 7 (3.8%) positive results were obtained by EIA method, 8 (4.3%) by ICG method, and 14 (7.6%) by rRT-PCR method. By accepting the rRT-PCR as a reference method, the sensitivity and specificity of the EIA method were determined as 50% and 100% and of the ICG method as 57% and 100%, respectively. The numbers and percentages of positivity for rotavirus, sapovirus, astrovirus and adenovirus, including coinfections were 30 (16.3%), 5 (2.7%), 2 (1%), 1 (0.5%), respectively. In this study, it was determined that norovirus, alone or together with other viral ag...
Source: Mikrobiyoloji Bulteni - Category: Microbiology Authors: Source Type: research