Evaluation of Anticancer Potential of Flavones from < em > Rhamnus alaternus < /em > against B16F10 Melanoma Cells

In this study, we want to investigate the anticancer activity of an enriched total oligomers flavonoids (TOF) extract of R. alaternus in melanoma cells. First, TOF was exhibited to be rich in flavones. We revealed that this extract reduced proliferation and increased of sub-G1 and S phase cells built-up in B16-F10 cells in a dose-related manner. Moreover, In Vivo, TOF reduced tumor volume and weight with percentages of inhibition of 92.4% and 92.9%, respectively. R. alaternus was also found to be effective in reducing the level of pro-inflammatory cytokine IL-6 during metastasis. Level of TH1 cytokine, such as IL-2, was significantly enhanced by TOF treatment. Indeed, the histological examination of the tumor revealed the absence of mitoses and the presence of numerous melanin pigmented macrophage cells in the R. alaternus extract-treated group that could be explained by the induction of macrophage activation and by the arrest of the cell cycle in the Sub-G1 and S phases.PMID:34783289 | DOI:10.1080/01635581.2021.2004171

Source: Nutrition and Cancer - November 16, 2021 Category: Cancer & Oncology Authors: Ines Bouhlel Chatti Yosr Krichen Mouna Maatouk Aida Lahmar Sonia Ghoul Mazgar Rim Kammoun Sihem Safta Skhiri Leila Chekir Ghedira Mounira Krifa Source Type: research