Effect of plant systemic resistance elicited by biological and chemical inducers on the colonization of the lettuce and basil leaf apoplast by < em > Salmonella enterica < /em >

Appl Environ Microbiol. 2021 Oct 6:AEM0115121. doi: 10.1128/AEM.01151-21. Online ahead of print.ABSTRACTMitigation strategies to prevent microbial contamination of crops are lacking. We tested the hypothesis that induction of plant systemic resistance by biological (ISR) and chemical (SAR) elicitors reduces endophytic colonization of leaves by Salmonella enterica serovars Senftenberg and Typhimurium. S. Senftenberg had greater endophytic fitness than S. Typhimurium in basil and lettuce. The apoplastic population sizes of serovars Senftenberg and Typhimurium in basil and lettuce, respectively, were significantly reduced approximately 10- to 100-fold by root treatment with microbial inducers of systemic resistance compared with the H2O treatment. Rhodotorula glutinis effected the lowest population increase of S. Typhimurium in lettuce (120-fold) and S. Senftenberg in basil leaves (60-fold) compared with the H2O treatment over 10 days post-inoculation. Trichoderma harzianum and Pichia guilliermondii did not have any significant effect on S. Senftenberg in the basil apoplast. The chemical elicitors acidobenzolar-S-methyl and DL-β-amino-butyric acid inhibited S. Typhimurium multiplication in the lettuce apoplast 10- and 2-fold, respectively, compared with H2O-treated plants. All ISR and SAR inducers applied to lettuce roots in this study increased leaf expression of the defense gene PR1, as did Salmonella apoplastic colonization in H2O-treated lettuce plants. Remarkably, both aci...
Source: Applied and Environmental Microbiology - Category: Microbiology Authors: Source Type: research